Initial Strain Research Articles

Environment-assisted cracking of AZ31 magnesium alloy in a borate buffer solution containing ammonium thiocyanate under various potentials

The environment-assisted cracking behavior of AZ31 magnesium alloy was investigated using tensile tests at cathodic and corrosion potentials in a Na2B4O7∙10H2O solution containing NH4SCN and in air. Although the mechanical properties of the AZ31 with an initial strain rate of 10−4 s−1 were independent of the environments, those degraded in the solution with an initial strain rate of 10−6 s−1. The total elongation became smaller at higher potentials. The average hydrogen absorption rate increased with a positive potential shift. These facts imply that environment-assisted cracking became more severe under relatively high potential owing to corrosion accompanied by enhanced hydrogen absorption.

Enhanced predictive method for pipeline strain demand subject to permanent ground displacements with internal pressure & temperature: a finite difference approach

Pipelines subject to ground deformations generated by geohazard loads carry high importance on pipeline analysis, design, and assessment due to risk of structural damage or failure. Additionally, internal pressure and temperature variation within an operating pipe induce additional strains in combination with pipe strains generated by ground displacement. In this study, an enhanced predictive method is proposed founded upon methods employed by Zheng et al. (2022) to assess pipeline behaviour subject to permanent ground displacement, while considering effects of internal operating pressure and temperature variation. The finite difference-based method previously proposed for strain analysis of buried steel pipes subject to ground movement ignores the effects of internal pressure and/or temperature loading, limiting the applicability of this approach to exclude the operating conditions of pipelines. To address this limitation, the proposed enhanced method accounts for the initial thermal strains and biaxial stress state in the pipe due to hoop stress generated by internal pressure. These additional strains are considered within the expressions of internal axial force and bending moment, derived based on the actual stress distribution on the pipe cross-section. The accuracy of the proposed method is validated against the finite element method (FEM) with respect to results of strain and deformation demand using several indicative case studies. This research provides an effective method of incorporating temperature and internal pressure loads of pipelines subject to permanent ground displacements of varying types, magnitudes, and directions.

Identification and Functional Analysis of Two Novel Genes-Geranylgeranyl Pyrophosphate Synthase Gene (AlGGPPS) and Isopentenyl Pyrophosphate Isomerase Gene (AlIDI)-from Aurantiochytrium limacinum Significantly Enhance De Novo β-Carotene Biosynthesis in Escherichia coli.

Precursor regulation has been an effective strategy to improve carotenoid production and the availability of novel precursor synthases facilitates engineering improvements. In this work, the putative geranylgeranyl pyrophosphate synthase encoding gene (AlGGPPS) and isopentenyl pyrophosphate isomerase encoding gene (AlIDI) from Aurantiochytrium limacinum MYA-1381 were isolated. We applied the excavated AlGGPPS and AlIDI to the de novo β-carotene biosynthetic pathway in Escherichia coli for functional identification and engineering application. Results showed that the two novel genes both functioned in the synthesis of β-carotene. Furthermore, AlGGPPS and AlIDI performed better than the original or endogenous one, with 39.7% and 80.9% increases in β-carotene production, respectively. Due to the coordinated expression of the 2 functional genes, β-carotene content of the modified carotenoid-producing E. coli accumulated a 2.99-fold yield of the initial EBIY strain in 12 h, reaching 10.99 mg/L in flask culture. This study helped to broaden current understanding of the carotenoid biosynthetic pathway in Aurantiochytrium and provided novel functional elements for carotenoid engineering improvements.

PRE-CLINICAL TESTING AND FINITE ELEMENT SIMULATIONS OF ORTHOPAEDIC IMPACTION INSTRUMENTS USED IN SEATING OF IMPLANTS

Orthopaedic impaction-instruments are used to drive implants into the bone of the patient. Pre-clinical experimental testing protocols and computer models of those are used to assess robustness and functional efficiency of such instruments. This generally involves impaction of the instrument mounted on a substrate that should represent the mechanics of the patient. In this study, the effects of the substrate on stressing of the impaction-instruments were investigated using dynamic finite element analysis. Model results were compared with experimental data from lab protocols, which have been derived to recreate the mechanics of cadaveric implantations, which represent clinical conditions.FEA models of selected experimental protocols were created in which a simplified instrument was impacted on substrates with varying material properties and boundary conditions. After impaction, the instrument settled into a modal vibration which then decayed over time. The resulting axial strain data from the computational model was compared to strain-gauge data collected from experimental measurements. Strain signal amplitude, frequency and decay were compared. The damping-ratio was derived from the decay of the strain signal.The computational model slightly over-predicted the initial experimental strain amplitudes in all cases, but the frequency of the cyclic strain signals matched. However, the model underestimated the experimentally measured rate of signal decay. Inclusion of implant seating and soft-tissue conditions had little effect on decay.Clinical failures of impaction-instruments may be related to multiple fatigue cycles for each impaction and should be modelled accurately to allow failure prediction. Any soft substrate results in an impedance mismatch at the instrument interface, which reflects the pressure wave and causes vibration with a frequency related to the speed-of-sound in the instrument, and its geometry. While this could be accurately modelled computationally, signal decay was underestimated. Further experimental quantification of energy losses will be important to understand vibration decay.

Mechanisms of types B and C serrations in the tensile flow curve of C-bearing high Mn steel

In the present study, the mechanisms of types B and C serrations in the tensile curves of C-bearing high Mn steel were investigated. Many tensile tests were performed at various temperatures (313–573 K) with two different initial strain rates (ε˙ini = 1 × 10−3 and 1 × 10−4/s). Type B serrations appeared in a lower tensile temperature range compared to type C serrations. Whereas a critical strain for type B serrations (ecB) was similar to yield strain regardless of tensile temperature and strain rate, a critical strain for type C serrations (ecC) increased with increasing tensile temperature, indicating the inverse PLC behavior of ecC. The inverse PLC behavior of ecC was explained by the intensified dynamic strain aging (DSA) model. The repetition of rapid aging and easy breakaway causes the discontinuous propagation of the PLC band with flickering, thereby resulting in type B serrations.

The influence of crustal strength on rift geometry and development – insights from 3D numerical modelling

Abstract. The lateral distribution of strength within the crust is non-uniform, dictated by crustal lithology and the presence and distribution of heterogeneities within it. During continental extension, areas of crust with distinct lithological and rheological properties manifest strain differently, influencing the structural style, geometry, and evolution of the developing rift system. Here, we use 3D thermo-mechanical models of continental extension to explore how pre-rift upper-crustal strength variations influence rift physiography. We model a 500×500×100 km volume containing 125 km wide domains of mechanically “strong” and “weak” upper crust along with two reference domains, based upon geological observations of the Great South Basin, New Zealand, where extension occurs parallel to the boundaries between distinct geological terranes. Crustal strength is represented by varying the initial strength of 5 km3 blocks. Extension is oriented parallel to the domain boundaries such that each domain is subject to the same 5 mm yr−1 extension rate. Our modelling results show that strain initially localises in the weak domain, with faults initially following the distribution of initial plastic strain before reorganising to produce a well-established network, all occurring in the initial 100 kyr. In contrast, little to no localisation occurs in the strong domain, which is characterised by uniform strain. We find that although faults in the weak domain are initially inhibited at the terrane boundaries, they eventually propagate through and “seed” faults in the relatively strong adjacent domains. We show characteristic structural styles associated with strong and weak crust and relate our observations to rift systems developed across laterally heterogeneous crust worldwide, such as the Great South Basin, New Zealand, and the Tanganyika Rift, East Africa.

Gibberellic acid overproduction in Fusarium fujikuroi using regulatory modification and transcription analysis.

Gibberellic acid (GA3), one of the natural diterpenoids produced by Fusarium fujikuroi, serves as an important phytohormone in agriculture for promoting plant growth. Presently, the metabolic engineering strategies for increasing the production of GA3 are progressing slowly, which seriously restricted the advancing of the cost-effective industrial production of GA3. In this study, an industrial strain with high-yield GA3 of F. fujikuroi was constructed by metabolic modification, coupling with transcriptome analysis and promoter engineering. The over-expression of AreA and Lae1, two positive factors in the regulatory network, generated an initial producing strain with GA3 production of 2.78g L-1. Compared with a large abundance of transcript enrichments in the GA3 synthetic gene cluster discovered by the comparative transcriptome analysis, geranylgeranyl pyrophosphate synthase 2 (Ggs2), and cytochrome P450-3 genes, two key genes that respectively participated in the initial and final step of biosynthesis, were identified to be downregulated when the highest GA3 productivity was obtained. Employing with a nitrogen-responsive bidirectional promoter, the two rate-limiting genes were dynamically upregulated, and therefore, the production of GA3 was increased to 3.02g L-1. Furthermore, the top 20 upregulated genes were characterized in GA3 over-production, and their distributions in chromosomes suggested potential genomic regions with a high transcriptional level for further strain development. The construction of a GA3 high-yield-producing strain was successfully achieved, and insights into the enriched functional transcripts provided novel strain development targets of F. fujikuroi, offering an efficient microbial development platform for industrial GA3 production. KEY POINTS: • Global regulatory modification was achieved in F. fujikuroi for GA3 overproduction. • Comparative transcriptome analysis revealed bottlenecks in GA specific-pathway. • A dynamically nitrogen-regulated bidirectional promoter was cloned and employed.

Axial compressive behavior of circular concrete-filled steel tube stub columns with steel slag coarse aggregate

Steel slag, an industrial waste by-product, has been used as an alternative sustainable aggregate material in construction applications to attempt to manage solid waste. In this context, this work conducts axial compression tests on 5 groups of 10 circular steel slag concrete-filled steel tube (SSCFST) stub columns to study the feasibility of using steel slag coarse aggregate in concrete-filled steel tubes. The failure mode, load–strain curve, axial bearing capacity, initial axial stiffness, critical strain, and lateral deformation coefficient of the columns are analyzed. Further, the effects of the steel slag replacement ratio and cross-section dimension on the axial compressive properties of the specimens are quantified. These test results are employed to verify the reliability of a finite element model of the circular SSCFST stub columns established using Abaqus software. Then, a parametric analysis is performed to investigate the factors influencing the axial compressive performance of circular SSCFST stub columns. Moreover, the prediction accuracy of the existing design methods for the axial bearing capacity of circular SSCFST stub columns is evaluated. The results show that the replacement ratio of steel slag influences the axial compressive properties of concrete-filled steel tubes negligibly: the axial bearing capacity and initial axial stiffness of the columns decrease only by 1.4% and 2.4% respectively, at a steel slag replacement ratio of 100%. Increasing the cross-section diameter from 160 mm to 180 and 200 mm improves the axial bearing capacity of the columns by 25.6% and 54.6% respectively and reduces the critical strain by 12.4% and 21.7% respectively. The confinement factor is still critical to affecting the axial compressive performance of circular SSCFST stub columns. Finally, the formulas proposed in standards GB 50936–2014 and AIJ-2008 can be employed to calculate the axial bearing capacity of SSCFST stub columns.

Thermal Effects on Shear Characteristics of Unbound Granular Materials under Drained Conditions

In this study, the thermally induced changes in the shear characteristics of unbound granular materials (UGMs) were investigated. A series of drained shear tests were conducted using a large-scale triaxial apparatus under controlled temperatures. The thermal dependence of the peak strength, critical strength, internal friction angle, and dilatancy of UGMs were investigated under different temperatures, initial mean effective stresses, and relative compaction. Dense UGMs tended to expand during heating and slightly contract during cooling. In the heating and cooling process, a nonlinear relationship was observed between thermal deformation and temperature. Thermal deformation was attributed to the deformation, movement, and rotation of soil particles and closely related to the initial mean effective stress and relative compaction. A thermal softening effect was observed during the shear test, and the peak strength decreased with increasing temperature. However, negligible thermal effects were observed relative to residual strength. The effect of temperature on the shear characteristics of UGMs was primarily related to the thermal expansion of pores in the soil skeleton. The relationship between peak strength and initial thermal volumetric strain was found to be exponential. Considering the thermal effect, a normalized semi-empirical model of the parabolic Hvorslev envelope for dense UGMs was proposed to supply guidelines for road base design.

Expansive spalling mechanism of concrete due to high temperature based on developed hygro-thermal-mechanical model by 3D-RBSM-TNM

Explosive spalling of concrete has been explained based on thermal stress and vapor pressure, and the mechanism of explosive spalling has been still discussed. In this study, the influence of several factors on the mechanism of explosive spalling due to high temperature was validated using the coupled analytical system: Rigid Body Spring Model-Truss Network Model (RBSM-TNM) based on hygro-thermal mechanical model. Truss Network Model can evaluate the heat transfer and vapor pressure transfer. The thermal stress and vapor pressure are applied to 3D-RBSM as an initial strain and initial stress, respectively. This proposed analysis can describe the complicated explosive spalling phenomena. Moreover, the accumulation of vapor pressure in the cracks and the change in mechanical property due to high temperature can be taken into account. In this paper, the influences of thermal stress, vapor pressure, vapor pressure transfer through cracks, and the change in mechanical property due to high temperature on the explosive spalling are precisely elucidated. As a result, it was confirmed that thermal stress causes the distributed cracks at the internal area while it does not involve the explosive spalling and a trigger of explosive spalling is the vapor pressure.

Modular Pathway Compartmentalization for Agroclavine Overproduction in Saccharomyces cerevisiae.

Agroclavine, which has anti-depressant activity and anti-Alzheimer effects, is the raw material used to synthesize ergo-based drugs. Although the production of agroclavine from Saccharomyces cerevisiae is possible, its yield is exceptionally low. The current study proposes a modular compartmentalization strategy for identifying and modifying the bottleneck step in agroclavine overproduction. The agroclavine synthetic pathway was reconstituted in yeast, and the best combination of Claviceps fusiformis EasA with Claviceps purpurea EasD/EasG was identified. According to the data on the expression and subcellular localization of agroclavine pathway proteins, the whole pathway was divided into two modules by chanoclavine-I. Separate enzyme distribution within the downstream module and low expression of DmaW and EasE in the upstream module were identified as the bottleneck steps in the pathway. The pathway efficiency was enhanced 2.06-fold when the downstream module was entirely anchored to the endoplasmic reticulum compartment. Increasing NADPH supply by overexpressing POS5 further improved the agroclavine yield by 27.4%. Altering the intracellular localization of DmaW from the peroxisome to the endoplasmic reticulum (ER) not only improved protein expression but also accelerated the accumulation of agroclavine by 59.9%. Integration of all modified modules into the host chromosome resulted in an improved yield of agroclavine at 101.6 mg/L with flask fermentation (a 241-fold improvement over the initial strain) and ultimately produced 152.8 mg/L of agroclavine on fed-batch fermentation. The current study unlocked the potential of S. cerevisiae in the advanced biosynthesis of ergot alkaloids. It also provides a promising strategy to reconstitute compartmentalized pathways.

Virus-Specific Stem Cell Memory CD8+ T Cells May Indicate a Long-Term Protection against Evolving SARS-CoV-2

Immune memory to SARS-CoV-2 is key for establishing herd immunity and limiting the spread of the virus. The duration and qualities of T-cell-mediated protection in the settings of constantly evolving pathogens remain an open question. We conducted a cross-sectional study of SARS-CoV-2-specific CD4+ and CD8+ T-cell responses at several time points over 18 months (30-750 days) post mild/moderate infection with the aim to identify suitable methods and biomarkers for evaluation of long-term T-cell memory in peripheral blood. Included were 107 samples from 95 donors infected during the periods 03/2020-07/2021 and 09/2021-03/2022, coinciding with the prevalence of B.1.1.7 (alpha) and B.1.617.2 (delta) variants in Bulgaria. SARS-CoV-2-specific IFNγ+ T cells were measured in ELISpot in parallel with flow cytometry detection of AIM+ total and stem cell-like memory (TSCM) CD4+ and CD8+ T cells after in vitro stimulation with peptide pools corresponding to the original and delta variants. We show that, unlike IFNγ+ T cells, AIM+ virus-specific CD4+ and CD8+ TSCM are more adequate markers of T cell memory, even beyond 18 months post-infection. In the settings of circulating and evolving viruses, CD8+ TSCM is remarkably stable, back-differentiated into effectors, and delivers immediate protection, regardless of the initial priming strain.

Deformation-induced homogenization of the multi-phase senary high-entropy alloy MoNbTaTiVZr processed by high-pressure torsion

Dendritic microstructures are frequently observed in as-solidified refractory high-entropy alloys (RHEAs), and their homogenization typically requires a long-term heat treatment at extremely high temperatures. High-pressure torsion (HPT) has been shown to be capable of mixing immiscible systems at room temperature, and therefore represents a promising technique for homogenizing dendritic RHEAs. In this work, the as-solidified RHEA MoNbTaTiVZr was processed up to 40 revolutions by HPT. It was found that the dendritic microstructure was eliminated, resulting in a chemical homogeneity at a von Mises equivalent shear strain of about 400. The study of deformation mechanism showed an initial strain localization, followed by a co-deformation of the dendritic and interdendritic regions. In the co-deformation step, the Zr-rich interdendritic region gradually disappeared. The deformation-induced mixing also led to the formation of an ultra-fine grained (UFG) microstructure, exhibiting a grain size of approximately 50 nm. The microhardness increased from 500 HV in the as-solidified to 675 HV in the homogenized UFG state. The underlying mechanisms responsible for the microhardness enhancement, such as grain refinement and solid solution strengthening, were also discussed.

Genomic epidemiology and transmission dynamics of recurrent Clostridioides difficile infection in Western Australia

Recurrent cases of Clostridioides difficile infection (rCDI) remain one of the most common and serious challenges faced in the management of CDI. The accurate distinction between a relapse (caused by infection with the same strain) and reinfection (caused by a new strain) has implications for infection control and prevention, and patient therapy. Here, we used whole-genome sequencing to investigate the epidemiology of 94 C. difficile isolates from 38 patients with rCDI in Western Australia. The C. difficile strain population comprised 13 sequence types (STs) led by ST2 (PCR ribotype (RT) 014, 36.2%), ST8 (RT002, 19.1%) and ST34 (RT056, 11.7%). Among 38 patients, core genome SNP (cgSNP) typing found 27 strains (71%) from initial and recurring cases differed by ≤ 2 cgSNPs, suggesting a likely relapse of infection with the initial strain, while eight strains differed by ≥ 3 cgSNPs, suggesting reinfection. Almost half of patients with CDI relapse confirmed by WGS suffered episodes that occurred outside the widely used 8-week cut-off for defining rCDI. Several putative strain transmission events between epidemiologically unrelated patients were identified. Isolates of STs 2 and 34 from rCDI cases and environmental sources shared a recent evolutionary history, suggesting a possible common community reservoir. For some rCDI episodes caused by STs 2 and 231, within-host strain diversity was observed, characterised by loss/gain of moxifloxacin resistance. Genomics improves discrimination of relapse from reinfection and identifies putative strain transmission events among patients with rCDI. Current definitions of relapse and reinfection based on the timing of recurrence need to be reconsidered.

Fine phase mixtures in one-dimensional non-convex elastodynamics

We show that fine phase mixtures arise in the initial-boundary value problem for a class of equations of non-convex elastodynamics in one space dimension. Specifically, we prove that there are infinitely many local-in-time Lipschitz weak solutions to such a problem that exhibit immediate fine-scale oscillations of the strain whenever the range of the initial strain has a nonempty intersection with the elliptic regime. Consequently, such solutions are nowhere C1 in the part of the space-time domain with fine phase mixtures, but are smooth in the other part of the domain.

Inhibited Crack Development by Compressive Strain in Perovskite Solar Cells with Improved Mechanical Stability.

Metal halide perovskites are promising as next-generation photovoltaic materials, but stability issues are still a huge obstacle to their commercialization. Here, the formation and evolution of cracks in perovskite films during thermal cycling, which affect their mechanical stability, are investigated. Compressive strain is employed to suppress cracks and delamination by in situ formed polymers with low elastic modulus during crystal growth. The resultant devices pass the thermal-cycling qualification (IEC61215:2016), retaining 95% of the initial power conversion efficiency (PCE) and compressive strain after 230 cycles. Meanwhile, the p-i-n devices deliver PCEs of 23.91% (0.0805 cm2 ) and 23.27% (1 cm2 ). The findings shed light on strain engineering with respect to their evolution, which enables mechanically stable perovskite solar cells.

Unusual grain-size effects on tensile deformation behavior of twinning-induced plasticity steel with low Mn content

In this work, the effects of grain refinement on the tensile properties, dynamic strain aging (DSA) and twinning behavior of Fe–16Mn-0.6C steel, which has relatively low manganese content, were investigated using a room-temperature monotonic tensile test, OM, XRD, EBSD and TEM. Results show that the yield strength, tensile strength and elongation of the steel increase with the decrease of grain size. Grain refinement can significantly increase the serration amplitude and local strain concentration of DSA, thereby enhancing the DSA-strengthening effect. The microstructure observations show that with the formation of plentiful twins, the twinning capacity of coarse-grained steel is exhausted prematurely at initial strains. Although the twinning behavior of fine-grained steel is retarded in the early stage of deformation, a large number of fine and dense twinning structures can be produced continuously at high strains. This unique twinning behavior and enhanced DSA in the fine-grained steel maintain the strain-hardening capacity of the steel, which delays the plastic instability and hence brings about simultaneous increases in strength and ductility.

β-Cryptoxanthin Production in Escherichia coli by Optimization of the Cytochrome P450 CYP97H1 Activity.

Cytochromes P450, forming a superfamily of monooxygenases containing heme as a cofactor, show great versatility in substrate specificity. Metabolic engineering can take advantage of this feature to unlock novel metabolic pathways. However, the cytochromes P450 often show difficulty being expressed in a heterologous chassis. As a case study in the prokaryotic host Escherichia coli, the heterologous synthesis of β-cryptoxanthin was addressed. This carotenoid intermediate is difficult to produce, as its synthesis requires a monoterminal hydroxylation of β-carotene whereas most of the classic carotene hydroxylases are dihydroxylases. This study was focused on the optimization of the in vivo activity of CYP97H1, an original P450 β-carotene monohydroxylase. Engineering the N-terminal part of CYP97H1, identifying the matching redox partners, defining the optimal cellular background and adjusting the culture and induction conditions improved the production by 400 times compared to that of the initial strain, representing 2.7 mg/L β-cryptoxanthin and 20% of the total carotenoids produced.

Recurrent spontaneous Escherichia coli meningitis in an adult: a case report.

The aim of this study was to characterize an unusual case of spontaneous, community-acquired Escherichia coli meningitis in an adult presenting to a general hospital in Kenya, where initial clinical recovery was followed by reinfection with an MDR, hospital-acquired strain. An adult presented to a hospital in Kenya with meningitis symptoms. E. coli was cultured from CSF. Treatment with ceftriaxone was successful; however, the patient relapsed a few days later. E. coli was cultured from CSF and blood during the reinfection episode, though the patient died during admission. We sequenced the isolates using Illumina MiSeq and performed antimicrobial susceptibility testing, fitness and virulence assays on the bacteria. The E. coli isolates from the two episodes were found to be distinct: the initial strain was ST88, serotype O8 H17 while the subsequent episode was caused by an ST167, serotype O101 H5 MDR strain. The ST88 strain was susceptible to all drugs except ampicillin and amoxicillin/clavulanate while the ST167 strain was MDR, including to all β-lactam drugs due to the presence of the carbapenemase gene bla NDM-5. The hospital-acquired ST167 strain was also resistant to newer drugs such as cefiderocol and eravacycline, which are currently not available locally, and had overall lower fitness and virulence in vitro compared with the initial infecting strain. Though less fit and virulent in vitro, the MDR strain was fatal, suggesting that host factors, rather than bacterial virulence, may have been of greater importance in this patient's outcome.

Dynamic and balanced regulation of the thrABC operon gene for efficient synthesis of L-threonine.

L-threonine is an essential amino acid used widely in food, cosmetics, animal feed and medicine. The thrABC operon plays an important role in regulating the biosynthesis of L-theronine. In this work, we systematically analyzed the effects of separating thrAB and thrC in different proportions on strain growth and L-threonine production in Escherichia coli firstly. The results showed that higher expression of thrC than thrAB enhanced cell growth and L-threonine production; however, L-threonine production decreased when the thrC proportion was too high. The highest L-threonine production was achieved when the expression intensity ratio of thrAB to thrC was 3:5. Secondly, a stationary phase promoter was also used to dynamically regulate the expression of engineered thrABC. This strategy improved cell growth and shortened the fermentation period from 36h to 24h. Finally, the acetate metabolic overflow was reduced by deleting the ptsG gene, leading to a further increase in L-threonine production. With these efforts, the final strain P 2.1 -2901ΔptsG reached 40.06g/L at 60h fermentation, which was 96.85% higher than the initial control strain TH and the highest reported titer in shake flasks. The maximum L-threonine yield and productivity was obtained in reported fed-batch fermentation, and L-threonine production is close to the highest titer (127.30g/L). In this work, the expression ratio of genes in the thrABC operon in E. coli was studied systematically, which provided a new approach to improve L-threonine production and its downstream products.