Initial Slaughter Research Articles

Effects of different starch structures on energy metabolism in pigs

BackgroundStarch is a major component of carbohydrates and a major energy source for monogastric animals. Starch is composed of amylose and amylopectin and has different physiological functions due to its different structure. It has been shown that the energy supply efficiency of amylose is lower than that of amylopectin. However, there are few studies on the effect of starch structure on the available energy of pigs. The purpose of this study was to measure the effect of different structures of starch in the diet on the net energy (NE) of pigs using a comparative slaughter method and to establish a prediction equation to estimate the NE of starch with different structures. Fifty-six barrows (initial BW 10.18 ± 0.11 kg) were used, and they were housed and fed individually. Pigs were divided into 7 treatments, with 8 replicates for each treatment and 1 pig for each replicate. One of the treatments was randomly selected as the initial slaughter group (ISG). Pigs in the remaining treatments were assigned to 6 diets, fed with basic diet and semi-pure diets with amylose/amylopectin ratio (AR) of 3.09, 1.47, 0.25, 0.15 and 0.12, respectively. The experiment lasted for 28 d.ResultsResults showed that compared with the high amylose (AM) groups (AR 3.09 and 1.47), the high amylopectin (AP) group (AR 0.15) significantly increased the final BW, average daily weight gain and average daily feed intake of pigs (P < 0.05), but the F:G of the AM group was lower (P < 0.01). In addition, AR 0.15 and 0.12 groups have higher (P < 0.01) nutrient digestibility of dry matter, crude protein, gross energy and crude ash. Meanwhile, compared with other groups, AR 0.15 group has a higher (P < 0.05) NE intake and energy retention (RE). The regressive equation for predicting with starch structures was established as RE = 1,235.243 − 48.298AM/AP (R2 = 0.657, P = 0.05).ConclusionsIn conclusion, NE intake and RE of pigs augmented with the increase of dietary amylopectin content, indicating that diets high in amylopectin were more conducive to promoting the growth of pigs in the late conservation period.

Effects of dietary energy density and supplemental rumen undegradable protein on intake, viscera, and carcass composition of lambs recovering from nutritional restriction.

Variation in nutrition is a key determinant of growth, body composition, and the ability of animals to perform to their genetic potential. Depending on the quality of feed available, animals may be able to overcome negative effects of prior nutritional restriction, increasing intake and rates of tissue gain, but full compensation may not occur. A 2 × 3 × 4 factorial serial slaughter study was conducted to examine the effects of prior nutritional restriction, dietary energy density, and supplemental rumen undegradable protein (RUP) on intake, growth, and body composition of lambs. After an initial slaughter (n = 8), 124 4-mo-old Merino cross wethers (28.4 ± 1.8 kg) were assigned to either restricted (LO, 500 g/d) or unrestricted (HI, 1500 g/d) intake of lucerne and oat pellets. After 8 wk, eight lambs/group were slaughtered and tissue weights and chemical composition were measured. Remaining lambs were randomly assigned to a factorial combination of dietary energy density (7.8, 9.2, and 10.7 MJ/kg DM) and supplemental RUP (0, 30, 60, and 90 g/d) and fed ad libitum for a 12- to 13-wk experimental period before slaughter and analysis. By week 3 of the experimental period, lambs fed the same level of energy had similar DMI (g/d) and MEI (MJ/d) (P > 0.05), regardless of prior level of nutrition. Restricted-refed (LO) lambs had higher rates of fat and protein gain than HI lambs (P < 0.05) but had similar visceral masses (P > 0.05). However, LO lambs were lighter and leaner at slaughter, with proportionally larger rumens and livers (P < 0.05). Tissue masses increased with increasing dietary energy density, as did DMI, energy and nitrogen (N) retention (% intake), and rates of protein and fat gain (P < 0.05). The liver increased proportionally with increasing dietary energy density and RUP (P < 0.05), but rumen size decreased relative to the empty body as dietary energy density increased (P < 0.05) and did not respond to RUP (P > 0.05). Fat deposition was greatest in lambs fed 60 g/d supplemental RUP (P < 0.05). However, lambs fed 90 g/d were as lean as lambs that did not receive supplement (P0, P > 0.05), with poorer nitrogen retention and proportionally heavier livers than P0 lambs (P < 0.05). In general, visceral protein was the first tissue to respond to increased intake during refeeding, followed by non-visceral protein and fat, highlighting the influence of differences in tissue response over time on animal performance and body composition.

PENGARUH PENGGUNAAN BIOSUPLEMEN MENGANDUNG BAKTERI PROBIOTIK SELULOLITIK ASAL RAYAP (Termites sp.) TERHADAP PRODUKTIVITAS ITIK BALI JANTAN

The research aims at analyzing the effect use of probiotic cellulolytic biosuplement termite (Termites sp.) tothe productivity of male bali ducks. Bacteria isolates used were first and second preeminent probiotic cellulolyticbacteria isolated derived from termites code BR3.3 and BR3.5. It was designed using a Completely RandomizedDesign with five treatments and three replicates consists of: basal ration without biosuplement (R0), ration basalwith biosupplement but without additional probiotic cellulolytic bacteria inoculant preeminent termites (RBCT0),RBCT1= basal ration with biosuplement probiotic cellulolytic bacteria inoculant preeminent termites 1, RBCT2=basal ration with biosuplement probiotic cellulolytic bacteria inoculant preeminent termites 2 and RBCT1-2= basalration with biosuplement probiotic cellulolytic bacteria inoculant preeminent termites 1 and 2. The result showedthat the initial body weight, slaughter weight and carcass percentage had no significant differences (P&gt;0.05). Incontrast, final body weight, body weight gain, feed consumption, Feed Conversion Ratio and meat total cholesterolwere significantly affected (P&lt;0,05) to the result. It can be concluded that use of basal ration supplemented withbiosupplement probiotic cellulolytic bacteria inoculant preeminent termites 2 (RBCT2) can increase productivity ofbali duck at the age of 2 up to10 weeks.

Increasing zinc levels in phytase-supplemented diets improves the performance and nutrient utilization of broiler chickens

Three hundred and thirty-six day-old Ross-308 male broiler chicks were used in a 35-day trial to investigate the effect of different concentrations of dietary Zn and phytase on broiler performance and energy utilization. Twelve day-old birds were used for the initial slaughter group to provide baseline body compositional data, while the remaining 324 birds were randomly distributed to six experimental diets. The treatments consisted of a 3 x 2 factorial arrangement with three levels of Zn (low, mid, and high; 30, 40, and 50 mg/kg, respectively) and two levels (0, 500 FTU/kg) of microbial phytase. Each dietary treatment was fed to 6 cages (9 birds/cage). Low Zn diet significantly decreased feed intake and body weight gain at days 1-24. Phytase supplementation improved body weight gain at d 24, irrespective of Zn level. The digestibility of P was improved in birds fed high-Zn diet with phytase supplementation, and the reverse was the case for Fe and Zn digestibility. High dietary Zn increased the Zn and Fe deposition in liver. The activity of AP, Ca-ATPase and Mg-ATPase in the jejunum was high in the phytase supplemented mid-level Zn diet. Phytase supplemented to the mid and high level Zn diets significantly improved most energy utilization parameters. This result indicate that the Zn concentrations used in this study were not inhibitory to phytase activity and broiler performance. Therefore, it can be concluded that dietary zinc level in phytase-supplemented diets could be increased up to 50 mg/kg without any negative effect on phytase-mediated broiler response.Keywords: Bone quality, net energy, nutrient utilization

Proporção e crescimento alométrico dos componentes não carcaça de cordeiros Texel abatidos com diferentes pesos

The objective of this study was to evaluate the proportion regarding the empty body weight, and the allometric growth of non-carcass components of Texel lambs finished in feedlot and slaughtered at different weights. It was used 30 male lambs, non-castrated and simple delivered. The lambs were weaned at 50 day-old, individually confined and distributed in five treatments with six repetitions and each treatment with a predetermined slaughter weight (T23 – initial slaughter, T25, T30, T35 and T40 kg body weight). The proportions of lung+trachea, abomasum, small intestine, large intestine, head and legs decreased and the proportion of testicles increased linearly according to the improvement on the slaughter weight of lambs. The body components as liver, diaphragm, rumen, reticulum, omasun, skin, tongue, testicles, internal fat and kidney fat present late development. On the other way, lung+trachea, esophagus, heart, pancreas, kidneys, spleen, abomasum, small intestine, large intestine, blood, head, feet and penis are body components that present early development.

Tissue composition and allometric growth of tissues from commercial cuts and carcass of Texel lambs slaughtered with different weights

In this study, we evaluated the tissue composition, the ratios between different issues, and the allometric growth of tissues from the commercial cuts neck, shoulder, rib, and leg, and of the carcass of Texel lambs. Thirty uncastrated male lambs born from single calvings were used in this experiment. Lambs were weaned, confined individually, and distributed into five treatments with six replications. Each treatment consisted of a predefined slaughter weight (T23 – initial slaughter, T25, T30, T35, and T40). The proportion of bone decreased linearly in the shoulder, rib, and legs, but remained constant in the neck. The proportion of muscle decreased in the rib, whereas in the other cuts it remained constant. The percentage of fat increased as the slaughter weight of the lambs was increased, for all cuts. In all cuts and in the carcass, bone and muscle showed early growth (b &amp;lt; 1), whereas fat displayed late growth (b &amp;gt; 1). The proportion of bone decreased and fat increased linearly in the carcass, and the proportion of muscle was not influenced by the slaughter weight of the lambs. The muscle/fat ratio decreased in all cuts, and consequently in the half-carcass, as the slaughter weight was increased. The muscle/ bone ratio increased in the shoulder, rib, leg, and half-carcass, but there was no significant effect on the neck. The bone and muscle tissues showed early growth, whereas the fat had late growth in the different commercial cuts and carcass of Texel lambs. The tissue growth pattern of the shoulder may be representative of the allometric growth of the different carcass tissues. An increase in the slaughter weight of Texel lambs leads to a reduction of the muscle/fat ratio, due to the higher deposition of fat in the carcass of these animals.

Effects of in utero heat stress on postnatal body composition in pigs: I. Growing phase.

Environmentally induced heat stress (HS) negatively influences production variables in agriculturally important species. However, the extent to which HS experienced in utero affects nutrient partitioning during the rapid lean tissue accretion phase of postnatal growth is unknown. Study objectives were to compare future whole-body tissue accretion rates in pigs exposed to differing in utero and postnatal thermal environments when lean tissue deposition is likely maximized. Pregnant sows were exposed to thermoneutral (TN; cyclical 15°C nighttime and 22°C daytime; n = 9) or HS (cyclical 27°C nighttime and 37°C daytime; n = 12) conditions during their entire gestation. Twenty-four offspring from in utero TN (IUTN; n = 6 gilts and 6 barrows; 30.8 ± 0.2 kg BW) and in utero HS (IUHS; n = 6 gilts and 6 barrows; 30.3 ± 0.2 kg BW) were euthanized as an initial slaughter group (ISG). Following the ISG, 48 pigs from IUTN (n = 12 gilts and 12 barrows; 34.1 ± 0.5 kg BW) and IUHS (n = 12 gilts and 12 barrows; 33.3 ± 0.3 kg BW) were exposed to constant HS (34.1 ± 2.4°C) or TN (21.5 ± 2.0°C) conditions until they reached 61.5 ± 0.8 kg BW, at which point they were sacrificed and their whole-body composition was determined. Homogenized carcasses were analyzed for N, crude fat, ash, water, and GE content. Data were analyzed using PROC MIXED in SAS 9.3. Rectal temperature and respiration rate increased (P < 0.01) during postnatal HS compared to TN (39.4 vs. 39.0°C and 94 vs. 49 breaths per minute, respectively). Regardless of in utero environment, postnatal HS reduced (P < 0.01) feed intake (2.06 vs. 2.37 kg/d) and ADG (0.86 vs. 0.98 kg/d) compared to TN conditions. Postnatal HS did not alter water, protein, and ash accretion rates but reduced lipid accretion rates (198 vs. 232 g/d; P < 0.04) compared to TN-reared pigs. In utero environment had no effect on future tissue deposition rates; however, IUHS pigs from the ISG had reduced liver weight (P < 0.04; 17.9%) compared to IUTN controls. In summary, postnatal HS reduced adipose tissue accretion rates, but IUHS did not appear to impact either lean or adipose tissue accretion during this specific growth phase.

Effects of in utero heat stress on postnatal body composition in pigs: II. Finishing phase.

The detrimental effects of heat stress (HS) on animal productivity have been well documented. However, whether in utero HS interacts with a future thermal insult to alter tissue deposition during the finishing phase of pig growth is unknown. Study objectives were to compare the subsequent rate and quantity of whole-body tissue accretion in pigs exposed to differing in utero and postnatal thermal environments. Pregnant sows were exposed to thermoneutral (TN; cyclical 15°C nighttime and 22°C daytime; n = 9) or HS (cyclical 27°C nighttime and 37°C daytime; n = 11) conditions during their entire gestation. Twenty-four offspring from in utero TN (IUTN; n = 6 gilts and 6 barrows; 62.4 ± 0.7 kg BW) and in utero HS (IUHS; n = 6 gilts and 6 barrows; 61.9 ± 0.8 kg BW) were euthanized as part of an initial slaughter group (ISG). After the ISG, 48 pigs from IUTN (n = 12 gilts and 12 barrows; 66.1 ± 1.0 kg BW) and IUHS (n = 12 gilts and 12 barrows; 63.4 ± 0.7 kg BW) were exposed to constant HS (34.4 ± 1.8°C) or TN (22.7 ± 2.5°C) conditions until they reached 80.5 ± 1.5 kg BW, at which point they were sacrificed and their whole-body composition was determined. Homogenized carcasses were analyzed for N, crude fat, ash, water, and GE content. Data were analyzed using PROC MIXED in SAS 9.3. Rectal temperature and respiration rate increased during postnatal HS compared to TN (39.6 vs. 39.3°C and 92 vs. 58 breaths per minute, respectively; P < 0.01). Postnatal HS decreased (P < 0.01) feed intake (2.13 vs. 2.65 kg/d) and ADG (0.70 vs. 0.94 kg/d) compared to TN conditions, but neither variable was influenced by in utero environment. Whole-body protein and lipid accretion rates were reduced in HS pigs compared to TN controls (126 vs. 164 g/d and 218 vs. 294 g/d, respectively; P < 0.04). Independent of postnatal environments, IUHS reduced future protein accretion rates (16%; P < 0.01) and tended to increase lipid accretion rates (292 vs. 220 g/d; P < 0.07) compared to IUTN controls. The ratio of lipid to protein accretion rates increased (95%; P < 0.01) in IUHS pigs compared to IUTN controls. In summary, the future hierarchy of tissue accretion is altered by IUHS, and this modified nutrient partitioning favors adipose deposition at the expense of skeletal muscle during this specific phase of growth.

Effects of dietary soybean oil on pig growth performance, retention of protein, lipids, and energy, and the net energy of corn in diets fed to growing or finishing pigs1

The objectives of this experiment were 1) to determine if dietary soybean oil (SBO) affects the NE of corn when fed to growing or finishing pigs, 2) to determine if possible effects of dietary SBO on the NE of corn differ between growing and finishing pigs, and 3) to determine effects of SBO on pig growth performance and retention of energy, protein, and lipids. Forty-eight growing (initial BW: 27.3 ± 2.5 kg) and 48 finishing (initial BW: 86.0 ± 3.0 kg) barrows were used, and within each stage of growth, pigs were allotted to 1 of 6 groups. Two groups at each stage of growth served as an initial slaughter group. The remaining 4 groups were randomly assigned to 4 dietary treatments and pigs in these groups were harvested at the conclusion of the experiment. A low-lipid basal diet containing corn, soybean meal, and no added SBO and a high-lipid basal diet containing corn, soybean meal, and 8% SBO were formulated at each stage of growth. Two additional diets at each stage of growth were formulated by mixing 25% corn and 75% of the low-lipid basal diet or 25% corn and 75% of the high-lipid basal diet. Results indicated that addition of SBO had no effects on growth performance, carcass composition, or retention of energy, protein, and lipids but increased (P < 0.05) apparent total tract digestibility of acid hydrolyzed ether extract and GE. Addition of SBO also increased (P < 0.05) DE and NE of diets, but had no effect on the DE and NE of corn. Finishing pigs had greater (P < 0.05) growth performance and retention of energy, protein, and lipids than growing pigs. A greater (P < 0.05) DE and NE of diets was observed for finishing pigs than for growing pigs and the DE and NE of corn was also greater (P < 0.05) for finishing pigs than for growing pigs. In conclusion, addition of SBO increases the DE and NE of diets but has no impact on the DE and NE of corn. Diets fed to finishing pigs have greater DE and NE values than diets fed to growing pigs and the DE and NE of corn are greater for finishing pigs than for growing pigs.

Effects of dietary soybean hulls and wheat middlings on body composition, nutrient and energy retention, and the net energy of diets and ingredients fed to growing and finishing pigs1

The objectives of this experiment were 1) to determine the effect of dietary soybean hulls (SBH) and wheat middlings (WM) on body composition, nutrient and energy retention, and the NE of diets and ingredients fed to growing or finishing pigs and 2) to determine if finishing pigs use the energy in SBH and WM more efficiently than growing pigs. Forty growing barrows (initial BW: 25.4 ± 0.7 kg) and 40 finishing barrows (initial BW: 84.8 ± 0.9 kg) were randomly allotted to 5 groups within each stage of growth. Two groups at each stage of growth served as the initial slaughter group. The remaining pigs were randomly assigned to 3 dietary treatments and harvested at the conclusion of the experiment. The basal diet was based on corn and soybean meal and was formulated to be adequate in all nutrients. Two additional diets were formulated by mixing 70% of the basal diet and 30% SBH or 30% WM. In the growing phase, ADG, G:F, and retention of lipids were greater (P < 0.05) for pigs fed the basal diet than for pigs fed the diets containing SBH or WM. Retention of energy was also greater (P < 0.05) for pigs fed the basal diet than for pigs fed the SBH. In the finishing phase, pigs fed the SBH diet tended (P = 0.10) to have a greater ADG than pigs fed the WM diet, and energy retention was greater (P < 0.05) for pigs fed the basal diet than for pigs fed the WM diet. The NE of the basal diet fed to growing pigs was greater (P < 0.01) than the NE of the diets containing SBH or WM, and there was a tendency for a greater (P = 0.05) NE of the basal diet than of the other diets when fed to finishing pigs. The NE of SBH did not differ from the NE of WM in either growing or finishing pigs, and there was no interaction between ingredients and stage of growth on the NE of diets or ingredients. The NE of diets for growing pigs (1,668 kcal/kg) was not different from the NE of diets for finishing pigs (1,823 kcal/kg), and the NE of the diets containing SBH (1,688 kcal/kg) was not different from the NE of the diets containing WM (1,803 kcal/kg). Likewise, the NE of SBH (603 kcal/kg) did not differ from the NE of WM (987 kcal/kg). In conclusion, inclusion of 30% SBH or WM decreases the performance and nutrient retention in growing pigs but has little impact on finishing pigs. The NE of the diets decreases with the inclusion of SBH and WM, but the NE of diets and ingredients is not affected by the BW of pigs. The NE of SBH is not different from the NE of WM.

Characterizing growth and carcass composition differences in pigs with varying weaning weights and postweaning performance1

The unprecedented increase in litter size over the last decade has led to a perceived increase in the number of fallback pigs (Sus scrofa). However, there is little peer-reviewed data available regarding the biological differences between fallback pigs and their normal cohorts. Therefore, the objective of this experiment was to identify differences in the biology and physiology, and thus the growth and metabolism, between pigs with varying weaning weights (WW) and postweaning performance. To accomplish this objective, a total of 120 barrows (PIC C22/C29 × 337) were used in growth and comparative slaughter experiments. Pigs were selected from a population of 960 weanling pigs to represent the 10% lightest, median, and heaviest pigs at weaning (n = 40 pigs per WW category). Eight pigs from each WW category were harvested on d 5 postweaning as the initial slaughter group (ISG). The remaining 96 barrows were housed in individual crates, fed ad libitum quantities of a common diet during a 27-d growth study, and were harvested on d 33 or 34 postweaning. After the completion of the live animal component of the experiment, pigs within each WW category were further stratified into the slowest, median, or fastest 33% ADG categories. This resulted in a total of 9 treatments in a nested design. Fallback pigs were designated as those belonging to the slowest ADG category from either the lightest or median WW categories. Data were analyzed using the GLIMMIX procedure of SAS with the fixed effects of WW category and WW(ADG). Although feed intake was maximized (P < 0.0001) by WW(ADG) category, feed efficiency was not different (P = 0.30). When equalized per unit of BW, WW(ADG) category greatly affected (P < 0.02) eviscerated carcass, organ, and metabolic BW, but not (P = 0.28) empty BW. There were no differences (P > 0.12) in tissue nutrient concentrations, ratios, or energy content among pigs in the growth experiment. All tissue deposition rates, which were calculated as the difference between tissue nutrient concentrations of the growth experiment and initial slaughter groups, were maximized (P < 0.0002) by WW(ADG), even when equalized per unit of BW. In conclusion, WW and ADG affect tissue accretion rates, but not feed efficiency or carcass composition in nursery pigs.

The Effect Of Days On Feed and Beta‐Agonist Administration On The Expression Of Adipocyte‐Specific Regulatory Genes in Skeletal Muscle and Adipose Tissue of Finishing Heifers

The development of marbling (Intramuscular fat; IMF) is an economically relevant trait that is mediated at the molecular level of adipose tissue metabolism. The objective of the current research was to evaluate adipose‐specific regulatory genes in skeletal muscle IMF, and adipose depots of finishing heifers. Gastrocnemius Muscle (GM), Longissimus Dorsi Muscle (LM), and subcutaneous adipose tissue (AT) were collected after heifers were on feed for 79, 121, and 163 days and treated with (BAA) or without (Control) beta‐agonist for the final 30d of feeding, respectively. Quantitative Real‐Time PCR was used for gene expression analysis. Pref‐1 expression was highest in the initial slaughter group (P&lt;0.05), and decreased as time on feed increased. LM showed decreased Pref‐1 expression relative to GM indicating an increased IMF adipogenic capacity in this muscle depot. BAA treatment appeared to decrease Pref‐1 expression in both LM and GM. Subcutaneous AT showed increased Pref‐1 expression relative to LM (P&lt;0.05) when treatments within slaughter groups were combined, but when individual tissues and treatments were compared, there was no difference between LM and AT (P&gt;0.05), while the GM showed higher expression (P&lt;0.05) relative to AT and LM. This study showed a temporal Pref‐1 expression pattern that appears to be consistent with the paradigm of late development of the IMF depot.

The impact of ractopamine hydrochloride on growth and metabolism, with special consideration of its role on nitrogen balance and water utilization in pork production1

Two experiments were conducted to determine if ractopamine hydrochloride (RAC) could improve nutrient utilization and decrease water utilization, thus reducing the environmental footprint of hog operations. The tissue accretion experiment used comparative slaughter involving 120 barrows (95 ± 3 kg of BW), including 12 assigned to an initial slaughter group; the remaining pigs were slaughtered at 108 or 120 kg. Growth performance and nutrient retention were determined. The 15-d metabolism experiment consisted of 54 pigs (95 ± 3 kg of BW). Growth performance, feed and water intake, and urine and fecal output were measured. The metabolism experiment used 9 dietary treatments arranged as a 3 × 3 factorial: 3 quantities of RAC (0, 5, and 10 mg/kg) and 3 standardized ileal digestible-Lys:DE ratios (1.73, 2.14, and 2.63 g/Mcal of DE). The tissue accretion study was designed as a 3 × 3 × 2 factorial arrangement of treatments using the same 9 dietary treatments to include slaughter BW (108 and 120 kg of BW) as an additional factor. In the tissue accretion experiment, RAC had no effect on ADG, ADFI, or G:F (P>0.10). With increased Lys, G:F improved (P=0.029), but not ADG or ADFI (P>0.10). Protein deposition rates increased numerically (P=0.11); water deposition rates increased (P=0.050), whereas lipid deposition tended to decrease with RAC inclusion (P=0.055). With greater RAC and Lys, the pigs had improved ADG (P=0.002) and G:F (P<0.001) in the metabolism experiment. Daily water intake (P=0.017.) and water output (P=0.033) decreased with RAC inclusion. Lysine inclusion did not alter the water balance (P>0.10). Urinary N excretion (P<0.001), total N excretion (P=0.003), and the urine N:fecal N ratio (P<0.001) decreased with the addition of RAC; fecal N (P=0.008) increased with RAC inclusion. Retention of N improved with addition of RAC to the diet (P=0.003). With greater dietary Lys, fecal N was reduced (P<0.001). The pigs fed the 2.14 g of Lys/Mcal tended to have the least urinary N (P = 0.069) and total N excretion (P=0.086) and to have the greatest N retention (P=0.086) and urinary N:fecal N ratio (P=0.009). A RAC × Lys interaction was observed for N digestibility (P=0.001), excretion (P=0.001), and retention (P=0.002) and for fecal (P=0.001) and urinary N (P=0.036). By improving N and water utilization in finishing pigs, RAC-containing diets supplemented with sufficient Lys can reduce N excretion into the environment from swine facilities.

Net energy of soybean oil and choice white grease in diets fed to growing and finishing pigs1

The objectives of this experiment were 1) to determine the NE of soybean oil (SBO) and choice white grease (CWG) fed to growing and finishing pigs, 2) to evaluate the effects of inclusion rate of SBO on the NE by growing and finishing pigs, and 3) to determine if there is a difference in the NE of SBO and CWG between growing and finishing pigs. Forty-eight growing (initial BW: 22.13 ± 1.78 kg) and 48 finishing (initial BW: 84.17 ± 5.80 kg) barrows were used, and they were housed and fed individually. Within each stage of growth, pigs were allotted to 8 outcome groups of 6 barrows based on BW. Within each outcome group, pigs were randomly allotted to 1 of 6 groups. Two groups at each stage of growth served as an initial slaughter group. Pigs in the remaining groups were assigned to 4 dietary treatments and slaughtered at the conclusion of the experiment. The basal diet contained corn, soybean meal, and no supplemental lipids. Three additional diets were formulated by mixing 95% of the basal diet and 5% SBO, 90% of the basal diet and 10% SBO, or 90% of the basal diet and 10% CWG. Average daily gain and G:F for finishing pigs and apparent total tract digestibility of energy for growing and finishing pigs increased (linear, P < 0.05) with lipid content, but was not affected by lipid source. The lipid gain:protein gain ratio and the energy retention also increased (linear, P ≤ 0.05) with lipid content in growing and finishing pigs. There were no interactive effects between lipid content and stage of growth or between lipid source and stage of growth on the NE of diets and the NE of dietary lipids. The NE of diets increased (linear, P < 0.01) with increasing SBO (2,056, 2,206, and 2,318 kcal/kg for diets containing 0, 5, or 10% SBO). The NE of the diet containing 10% CWG (2,440 kcal/kg) was greater (P < 0.05) than the NE of the diet containing 10% SBO. The NE of diets was greater (P < 0.05) for finishing pigs than for growing pigs regardless of lipid content or source. The NE of SBO included at 5% (5,073 kcal/kg) was not different from the NE of SBO included at 10% (4,679 kcal/kg), but the NE of CWG (5,900 kcal/kg) was greater (P < 0.05) than the NE of SBO. The stage of growth had no impact on the NE of SBO or CWG. In conclusion, the NE of lipids is not affected by the content of dietary lipids, but the NE of CWG is greater than the NE of SBO.

Effects of winter growing program on visceral organ mass, composition, and oxygen consumption of beef steers during growing and finishing12

The purpose of this experiment was to investigate the effects of winter growing program on organ mass, composition, and oxygen consumption in beef steers. A total of 46 steers were used for the experiment. Four steers were randomly selected as an initial slaughter group. Remaining steers were randomly allotted to 1 of 4 treatment groups: 1) fed a high-concentrate diet for ad libitum intake (CF); 2) grazed on wheat pasture (WP); 3) fed a sorghum silage-based growing diet (SF); or 4) program fed a high-concentrate diet (PF). Steers in the WP, SF, and PF groups were managed to achieve approximately equal rates of BW gain during the growing phase. After the growing phase (112 d), steers in the WP, SF, and PF treatments were adapted to a high-concentrate diet for finishing. Steers from all treatments were slaughtered at a fat thickness of 1.27 cm as estimated by ultrasound. In addition, 6 steers from each treatment were randomly selected for slaughter at the end of the growing phase. Weights of all individual organs were measured and tissue samples of duodenum and liver collected. At the end of the growing phase, WP steers had greater (P < 0.05) small intestine, liver, and kidney mass than SF and PF steers. In contrast, mesenteric fat mass and total visceral fat content were greatest (P < 0.01) for PF, intermediate for SF, and least for WP steers. Mass of total viscera and total splanchnic tissues (TST) did not differ (P > 0.10) among treatments. At final slaughter, mass of mesenteric fat, total viscera, and TST were similar among treatments, but liver weights remained greatest (g/kg of empty BW; P < 0.01) for WP steers. There were no differences in oxygen consumption of duodenum or liver tissue on an equal weight basis (microL.min(-1.)g(-1)) at the end of either period. Growing program affected mass of components of the TST at the end of the growing phase, which contributed to differences in rate of splanchnic organ growth during finishing. We conclude that program feeding a high-concentrate diet during the growing phase may result in greater ADG and G:F during the subsequent finishing period compared with forage-based diets due to less accretion of visceral organ mass resulting in reduced maintenance energy requirements during finishing.

Energy utilization and growth performance of broilers receiving diets supplemented with enzymes containing carbohydrase or phytase activity individually or in combination

Energy utilization in broilers as influenced by supplementation of enzymes containing phytase or carbohydrase activities was investigated. Day-old male broilers (480) were allocated to four slaughter groups, thirty broilers in the initial slaughter group and 150 broilers in each of the final slaughter groups on days 7, 14 and 21. Broilers in each of the final slaughter groups were allocated to five treatments in a randomized complete block design, each treatment had six replicate cages of five broilers per replicate cage. The diets were maize-soyabean based with wheat as a source of NSP. The treatments were: (1) positive control that met nutrient requirements of the day-old broiler chick; (2) negative control (NC) deficient in metabolizable energy and P; (3) NC plus phytase added at 1000 FTU/kg; (4) NC plus cocktail of xylanase, amylase and protease (XAP); and (5) NC plus phytase and XAP. Gain and gain:food were depressed (P < 0.05) in the NC diet. Phytase improved (P < 0.05) gain at all ages and gain:food at days 0-14 and days 0-21. There was improvement (P < 0.01) in net energy for production, energy retained as fat and protein from days 0 to 14 and from days 0 to 21 in phytase-supplemented diet compared with the NC diet. Net energy for production was more highly correlated with performance criteria than metabolizable energy and may be a more sensitive energy utilization response criterion to use in evaluating broiler response to enzyme supplementation.

Whole Body Nutrient Accretion, Growth Performance and Total Tract Nutrient Retention Responses of Broilers to Supplementation of Xylanase and Phytase Individually or in Combination in Wheat-Soybean Meal Based Diets

Growth performance, total tract nutrient retention and whole body nutrient accretion rates responses of broilers to supplementation of enzymes containing phytase or xylanase activities were investigated using 300 broilers. At day old, 280 broilers were assigned to 5 dietary treatments which were: 1) positive control (PC) diet which met NRC (1994) nutrient requirement for broilers, 2) negative control (NC) diet which was marginally deficient in phosphorus and ME, 3) NC plus phytase added at 1,000FTU/kg, 4) NC plus xylanase added at 4,000U/kg and, 5) NC plus phytase and xylanase added at 1,000 and 4,000units/kg, respectively. Each treatment had 8 replicate cages with 7 birds per replicate cage. Comparative slaughter technique was used for determination of whole-body nutrient accretion rate. Twenty broilers with the same initial body weight as the 280 broiler chicks used in the growth trial made up the initial slaughter group killed at day 0. A final slaughter group of 40 birds, one bird from each cage, were slaughtered on day 21. The birds selected were those with body weight closest to the average body weight of the birds in each replicate cage. Phytase alone or combined with xylanase improved weight gain and bone ash (P<0.05). Phytase alone improved (P<0.5) total tract P retention and ME, phytase and xylanase combined improved (P<0.01) total tract dry matter and ME. Phytase alone improved (P<0.5) whole body daily accretion rates of dry matter, protein, fat, P, and Ca in comparison to NC treatment. Overall, phytase in wheat-based diet improved growth performance and whole body accretion of minerals and protein, the improvement in protein accretion is an indication of improvement in nutrient utilization resulting from phytase use.

Exigência de energia de mantença e composição corporal e do ganho de vacas de corte adultas de três grupos genéticos confinadas

Neste trabalho, foram estimadas as exigências de mantença de energia líquida (ELm) e metabolizável (EMm) e as mudanças na composição corporal e no ganho de vacas adultas não-gestantes e não-lactantes confinadas. Foram utilizadas 36 vacas, 12 Nelore (NEL), 12 F1 Pardo-Suíço-Nelore (PSN) e 12 F1 Red Angus-Nelore (RAN). As alterações na composição corporal dos animais durante o período experimental foram estimadas utilizando-se a técnica do abate comparativo: quatro animais de cada grupo genético foram alocados, aleatoriamente, nos tratamentos abate inicial (AI), alimentação restrita (AR) e alimentação ad libitum (AL). Os animais AR receberam, no período experimental, níveis de proteína e energia 15% acima da mantença e os AL, ração ad libitum, para ganho de 1,1 kg/dia. Os animais AI foram abatidos no início do experimento e os AR e AL 120 dias depois. Em todos os animais abatidos, foram estimadas as proporções corporais dos tecidos muscular, adiposo e ósseo, além dos conteúdos corporais de proteína, gordura e energia. Na estimação da ELm e da EMm, foram empregados modelos linear e não-linear. Os grupos genéticos não diferiram quanto às exigências de ELm e EMm, expressas em peso vivo em jejum (kcal/kg0,75/dia PVJ). Quando utilizados os modelos linear e não-linear, os valores de ELm encontrados foram de 80,7 e 83,6 kcal/kg0,75/dia PVJ e os de EMm, 109,9 e 128,0 kcal/kg0,75/dia PVJ, respectivamente. Os conteúdos de proteína por unidade de peso corpo vazio (PCVZ) e do ganho (GPCVZ) não diferiram entre os grupos genéticos, porém, os animais NEL apresentaram maior concentração de gordura e energia por kg PCVZ e GPVCZ para mesmo peso vivo.

Investigations of energy metabolism in weanling barrows: The interaction of dietary energy concentration and daily feed (energy) intake1

Much of our understanding of energy metabolism in the pig has been derived from studies in which the energy supply was controlled through regulated feed intake. In commercial situations, where ad libitum feeding is practiced, dietary energy concentration, but not daily feed intake, is under producer control. This study evaluated the interactive effects of dietary energy concentration and feeding level (FL) on growth, body composition, and nutrient deposition rates. Individually penned PIC barrows, with an initial BW of 9.5 +/- 1.0 kg, were allotted to 1 of 9 treatments in a 3 x 3 factorial arrangement plus an initial slaughter group (n = 6) that was slaughtered at the beginning of the trial. Three NE concentrations (low, 2.15; medium, 2.26; and high, 2.37 Mcal of NE/kg) and 3 feeding levels (FL: 100, 80, or 70% of ad libitum access to feed) were investigated. Daily feed allowance for the restricted-fed pigs was adjusted twice per week on a BW basis until completion of the experiment at 25 +/- 1 kg of BW. Average daily gain, ADFI, and G:F were unaffected by NE (mean = 572 g, 781 g, and 0.732 g/g, respectively). Average daily gain and ADFI, but not G:F, increased (P < 0.05) with FL. Empty body lipid concentration increased with dietary NE concentration and with FL; a significant (P < 0.01) interaction revealed that empty body lipid concentration increased most rapidly as ADFI increased on the highest energy diet. Empty body lipid concentration was greatest in pigs with ad libitum access to the high-NE diet. Empty body protein concentration decreased with increasing NE (P < 0.05) but was not affected by FL. Empty body protein deposition (PD) increased with increasing FL (P < 0.001), but not with NE. Empty body lipid deposition (LD) and the LD:PD ratio increased (P < 0.01) in pigs with ad libitum access to the high-NE diet. In conclusion, NE did not interact with FL on growth, body protein concentration, or PD, suggesting that the conclusions regarding energy utilization obtained from experiments using restricted feed intake may not easily be applied to pigs fed under ad libitum conditions. The interactive effects of NE and FL on body lipid concentration, LD, and the LD:PD ratio indicate that changes in dietary energy concentration alter the composition of gain without necessarily changing overall BW gain. Consequently, the composition of gain is an important outcome in studies on energy utilization.

Efeito da seleção para peso pós-desmame sobre a composição corporal de bovinos

Foi determinada a composição química corporal no peso de corpo vazio de 56 bovinos machos não-castrados selecionados ou não para peso aos 378 dias (P378), nascidos em 1999. Utilizaram-se animais dos grupos genéticos Nelore Seleção (NeS), Nelore Controle (NeC) e Caracu Seleção (Ca), distribuídos aleatoriamente em três grupos experimentais: AI - grupo de abate inicial; AR - grupo de alimentação restrita; e AL - grupo de alimentação ad libitum. No grupo de abate inicial, foram utilizados quatro animais por grupo genético e, nas categorias AR e AL, havia oito animais NeS e Ca e seis NeC. Após o período de adaptação, os animais do grupo AI foram abatidos e os demais foram submetidos ao ensaio de alimentação. O período experimental foi determinado pelo tempo de acabamento dos animais do grupo AL, ou seja, quando atingiram 4 mm de espessura de gordura, avaliada por ultra-som. Em cada grupo genético, à medida que o acabamento preconizado para os animais AL foi atingido, o animal AR com peso e condição corporal mais semelhante a esse no início do experimento também foi abatido. O efeito do grupo genético foi significativo, porém, não houve efeito da interação grupos genéticos × regimes alimentares sobre a maior parte das características estudadas. A seleção para peso pós-desmame não promoveu alterações indiretas na composição corporal. Animais Ca apresentaram porcentagens menores de gordura e maiores de proteína no corpo vazio, provavelmente em virtude do maior tamanho corporal desses animais.