Initial Peaks Research Articles

Ground Moving Target Detection and Imaging Using a Virtual Multichannel Scheme in HRWS Mode

Along-track multichannel synthetic aperture radar is usually used to achieve ground moving target detection and imaging. Nevertheless, there is a design dilemma between azimuth high resolution and wide swath (HRWS). To solve this problem in HRWS mode, we introduce a virtual multichannel (VMC) scheme. For each virtual channel, the low real pulse repetition frequency (PRF) improves the ability of resolving range ambiguity for wide-swath, and the high virtual PRF improves the capability of resolving Doppler ambiguity for azimuth high resolution. For multiple virtual channels, strong ground clutter is eliminated by the joint VMC processing. Furthermore, a detailed signal model of a moving target in the virtual channel is given, and the special false-peak effect in the azimuthal image is analyzed. Moreover, we propose a novel ground moving target processing method based on the VMC scheme and the clutter suppression interferometry (CSI) technique, which is called VMC-CSI. The integration of detection, location, velocity estimation, and imaging for ground moving targets can be achieved. Accounting for the unresolved main peak and false peak for a moving target, in the VMC-CSI method, we adopt a two-step scheme to estimate the radial velocity and along-track velocity, namely, rough estimation and precise estimation. Meanwhile, considering the same interferometric phases of the main peak and the false peak, we use false peaks first for the robustness of initial azimuth location estimation and remove false peaks afterward. Numerical simulations are provided for testing the effect of the false peak and the effectiveness of VMC-CSI.

Effects of Whey, Caseinate, or Milk Protein Ingestion on Muscle Protein Synthesis after Exercise

Whey protein (WP) is characterized as a “fast” protein and caseinate (CA) as a “slow” protein according to their digestion and absorption rates. We hypothesized that co-ingestion of milk proteins (WP and CA) may be effective for prolonging the muscle protein synthesis response compared to either protein alone. We therefore compared the effect of ingesting milk protein (MP) to either WP or CA alone on muscle protein synthesis after exercise in rats. We also compared the effects of these milk-derived proteins to a control, soy protein (SP). Male Sprague-Dawley rats swam for two hours. Immediately after exercise, one of the following four solutions was administered: WP, CA, MP, or SP. Individual rats were euthanized at designated postprandial time points and triceps muscle samples collected for measurement of the protein fractional synthesis rate (FSR). FSR tended to increase in all groups post-ingestion, although the initial peaks of FSR occurred at different times (WP, peak time = 60 min, FSR = 7.76%/day; MP, peak time = 90 min, FSR = 8.34%/day; CA, peak time = 120 min, FSR = 7.85%/day). Milk-derived proteins caused significantly greater increases (p < 0.05) in FSR compared with SP at different times (WP, 60 min; MP, 90 and 120 min; CA, 120 min). Although statistical analysis could not be performed, the calculated the area under the curve (AUC) values for FSR following this trend were: MP, 534.61; CA, 498.22; WP, 473.46; and SP, 406.18. We conclude that ingestion of MP, CA or WP causes the initial peak time in muscle protein synthesis to occur at different times (WP, fast; MP, intermediate; CA, slow) and the dairy proteins have a superior effect on muscle protein synthesis after exercise compared with SP.

Statistical analysis of electric field parameters for negative lightning in Malaysia

This paper presents a comparative study on the electric field and its derivative parameters of negative lightning in Malaysia and other regions. This study is the first in Malaysia where the parameters of negative electric field and its derivative are thoroughly analyzed. 104 negative lightning flashes containing 277 negative return strokes occurring within 10–100km from the measuring station and recorded during monsoon period in the state of Johor, Malaysia had been analyzed. It was found that 73% of the recorded flashes are multiple strokes with an average multiplicity of 2.6 strokes per flash. For first return strokes, the arithmetic mean (AM) of initial peak electric field and the AM of initial peak electric field derivative are 21.8V/m and 11.3V/m/µs, respectively. The initial peaks of electric field and its derivative for first return strokes are larger than those for the subsequent return strokes. Comparison of overall results with those obtained earlier in Sri Lanka, Germany, Sweden, Japan, Florida indicates that several electric field and its derivative parameters are affected by propagation media and geographical region. Similarity of results with other countries having the same climatic condition is also observed.

Properties of 500Hz air- and bone-conducted vestibular evoked myogenic potentials (VEMPs) in superior canal dehiscence.

To compare threshold and amplitude properties for air- (AC) and mastoid bone-conducted (BC) cervical (cVEMP) and ocular (oVEMP) vestibular evoked myogenic potentials in superior canal dehiscence (SCD). Thirteen patients (53±14yrs) clinically diagnosed with SCD were tested using AC 500Hz sound and BC 500Hz transmastoid vibration. Baseline intensities of 135dBpSPL and 138dBpFL respectively were used and reduced until the response amplitudes were less than 2.5 standard deviations of the prestimulus baseline mean. SCD VEMP amplitudes, response gradients and threshold parameters for the initial peaks for the cVEMP (ipsilateral) and the oVEMP (contralateral) were compared with results for normal subjects over a range of intensities. Despite higher amplitudes, reflex gradients against intensity for AC and BC cVEMPs were significantly less in SCD than normals (P≪0.001) while AC and BC oVEMP gradients were not significantly different between the groups. Abnormally low thresholds for AC 500Hz were present for 85% of cVEMPs and 62% of oVEMPs. Abnormally low BC 500Hz thresholds were seen for 33% of cVEMPs and 83% of oVEMPs. Amplitudes for AC 500Hz were compared over the 135-105dBpSPL intensity range. The cVEMP showed more pathologically large amplitude responses with the lower stimulus intensities (135dBpSPL: 5%, 105dBpSPL: 100%) whereas the oVEMP demonstrated high rates of amplitude increases for all intensities (129-111dBpSPL: 92%). The pattern of pathologically large amplitudes evoked by BC 500Hz was similar for both reflexes such that both cVEMPs and oVEMPs showed maximum prevalence of abnormally large responses around 117dBpFL (cVEMP: 58%, oVEMP: 83%). In SCD, both AC and BC evoked cVEMPs show evidence of saturation but this is not evident for oVEMPs. Both cVEMPs and oVEMPs show frequent abnormalities of amplitudes and thresholds in SCD compared to normal subjects but the sensitivities differed between measures. Previous evidence of saturation of cVEMP responses in SCD was confirmed. For diagnosis, AC cVEMP amplitudes at 105dBpSPL or AC-evoked oVEMP amplitudes both have over 90% sensitivity in separating SCD from normal responses.

DES14X3taz: A TYPE I SUPERLUMINOUS SUPERNOVA SHOWING A LUMINOUS, RAPIDLY COOLING INITIAL PRE-PEAK BUMP

ABSTRACT We present DES14X3taz, a new hydrogen-poor superluminous supernova (SLSN-I) discovered by the Dark Energy Survey (DES) supernova program, with additional photometric data provided by the Survey Using DECam for Superluminous Supernovae. Spectra obtained using Optical System for Imaging and low-Intermediate-Resolution Integrated Spectroscopy on the Gran Telescopio CANARIAS show DES14X3taz is an SLSN-I at z = 0.608. Multi-color photometry reveals a double-peaked light curve: a blue and relatively bright initial peak that fades rapidly prior to the slower rise of the main light curve. Our multi-color photometry allows us, for the first time, to show that the initial peak cools from 22,000 to 8000 K over 15 rest-frame days, and is faster and brighter than any published core-collapse supernova, reaching 30% of the bolometric luminosity of the main peak. No physical 56Ni-powered model can fit this initial peak. We show that a shock-cooling model followed by a magnetar driving the second phase of the light curve can adequately explain the entire light curve of DES14X3taz. Models involving the shock-cooling of extended circumstellar material at a distance of ≃400 R &#x02609; ?> are preferred over the cooling of shock-heated surface layers of a stellar envelope. We compare DES14X3taz to the few double-peaked SLSN-I events in the literature. Although the rise times and characteristics of these initial peaks differ, there exists the tantalizing possibility that they can be explained by one physical interpretation.

Abstract 30: Quantitative monitoring of EGFR mutations in urinary circulating tumor DNA enables non-invasive pharmacodynamic assessment of anti-EGFR drug response

Abstract Background: Acquisition of the EGFR T790M resistance mutation is a hallmark of disease progression in patients with metastatic EGFR mutant lung adenocarcinoma treated with first generation anti-EGFR inhibitors. Utilizing a single copy sensitivity mutation detection platform and implementing daily collection of urine samples, we sought to demonstrate the feasibility of detecting EGFR mutations in urinary ctDNA and understand mechanisms of resistance to targeted therapies in patients with EGFR-mutated lung adenocarcinoma. Methods: In a biomarker study of 100 patients with EGFR-mutant metastatic lung adenocarcinoma (34 patients enrolled), urine was collected at either daily or monthly time points up to 4 months prior to radiologic detection of progression on erlotinib, and at multiple time points post-progression on next line therapy. Urinary ctDNA was extracted by a method that preferentially isolates short, fragmented ctDNA. Quantitative analysis of EGFR activating mutations and T790M resistance mutation was performed using blocker technology and PCR enrichment coupled with NGS detection (MiSeq). Urine was collected daily after the initiation of second line anti-EGFR therapy in 10 patients. Early pharmacodynamic events that occur within the first hours to days of anti-EGFR therapy were further studied by quantitating ctDNA for EGFR exon 19deletions, L858R, and T790M. Results: Interim analysis was conducted on 34 patients receiving first line anti-EGFR therapy with erlotinib; twenty-two of 34 patients demonstrated radiographic progression. Analysis of longitudinal samples revealed that the EGFR T790M mutation was detected in the urine specimens of 15 out of 22 (68%) patients on erlotinib. All 10 patients who were positive for T790M mutation by tissue were also positive by urine. Urine testing identified five additional T790M-positive patients who had a high clinical suspicion of T790M progressive disease. Three of these patients were tissue negative but both plasma and urine positive for T790M. EGFR T790M was detected up to 15 weeks prior to radiolographic detection of progression on first line erlotinib. Early peaks in ctDNA on days 1-4 correlated with tumor lysis. An observed sustained decrease in mutational levels after week 1 of therapy confirms the cytostatic effect of the tyrosine kinase inhibitor (TKI). The size of the initial peaks in ctDNA for EGFR exon 19deletions, L858R, and T790M correlated with CT radiographic response after two cycles of therapy. Conclusion: We demonstrate that the T790M mutation can be successfully detected in urinary ctDNA months before progression on anti-EGFR TKIs. Urinary ctDNA testing identifies additional patients who potentially are eligible for anti-T790M treatment. Initial results from 10 patients demonstrated that kinetic changes in EGFR ctDNA mutational load after drug adminstration can be used with pharmacokinetic data to better understand dyanmic changes in tumor biology, drug bioavailability, and assist in early drug development. The clinical utility of daily kinetic monitoring of ctDNA in urine after drug adminstration is being further validated in a larger study. Citation Format: Hatim Husain, Karena Kosco, Saege Hancock, Errin Samuelsz, Shiloh Guerrero, Brian Woodward, Cecile Rose Vibat, Vlada Melnikova, Mark Erlander, Scott Lippman, Razelle Kurzrock. Quantitative monitoring of EGFR mutations in urinary circulating tumor DNA enables non-invasive pharmacodynamic assessment of anti-EGFR drug response. [abstract]. In: Proceedings of the AACR Precision Medicine Series: Integrating Clinical Genomics and Cancer Therapy; Jun 13-16, 2015; Salt Lake City, UT. Philadelphia (PA): AACR; Clin Cancer Res 2016;22(1_Suppl):Abstract nr 30.

Analysis of origin of measured 1/f noise in high-power semiconductor laser diodes far below threshold current

The 1/f noise is measured under the bias one tenth the threshold current of the InGaAs quantum well high-power semiconductor laser diodes (LDs). The noise origin is analyzed using the current and voltage 1/f noise and dynamic resistance characteristics. Then the relationship between the noise and the internal defect is analyzed according to the differences of LDs in the noise intensity and the fluctuation near the initial electrical derivative peak. The result shows that with currents 0.13mA–1mA, the dynamic resistance of the LDs is in the magnitude of hundreds of ohms, when the changing rates of both the noise intensity and the resistance reflect the typical features of the active region, while with currents 8mA–32mA, the dynamic resistance drops under 10Ω and its changing rate slows down, when the 1/f noise intensity trend shows the features of the contact resistance. Moreover, the electrical derivative of LDs with weaker noise fluctuates milder and has more conspicuous initial peaks, while the electrical derivative of other LDs fluctuates acuter and hardly shows distinct initial peaks. The results indicate that the 1/f noise from the active region can be measured under bias currents far lower than the threshold currents of the LDs, and it can indicate the defects in the active region and further the reliability of the device.

Wavelet Customization for Improved Fault-Location Quality in Power Networks

A procedure for computing customized wavelets suitable for identifying the location of a fault along the length of a transmission line is proposed. Measuring the time difference of arrival (TDOA) between two consecutive transient reflections or the TDOA between the two initial peaks of two synchronized voltage/current bus measurements provides an accurate fault-location estimate. Reliable TDOA estimates can be obtained only when the transformed fault signals consist of short/peaked pulses that arrive on a bus with a small group delay. Our customization method improves the “peakedness” and shortens the group delay of the transient signal by maximizing a suitable peakedness objective function. It is shown that this approach is superior in terms of accuracy compared to the “classic” (Daubechies) wavelet-based technique found in the literature. The proposed method is independent of the fault type and can be used to reduce the required data sampling rate while maintaining reliable TDOA estimates.

Site-specific temporal and spatial validation of a generic plant pest forecast system with observations of Bactrocera dorsalis (oriental fruit fly)

This study introduces a simple generic model, the Generic Pest Forecast System (GPFS), for simulating the relative populations of non-indigenous arthropod pests in space and time. The model was designed to calculate the population index or relative population using hourly weather data as influenced by developmental rate, high and low temperature mortalities and wet soil moisture mortality. Each module contains biological parameters derived from controlled experiments. The hourly weather data used for the model inputs were obtained from the National Center of Environmental Prediction Climate Forecast System Reanalysis (NCEP-CFSR) at a 38 km spatial resolution. A combination of spatial and site-specific temporal data was used to validate the GPFS models. The oriental fruit fly, Bactrocera dorsalis (Hendel), was selected as a case study for this research because it is climatically driven and a major pest of fruit production. Results from the GPFS model were compared with field B. dorsalis survey data in three locations: 1) Bangalore, India; 2) Hawaii, USA; and 3) Wuhan, China. The GPFS captured the initial outbreaks and major population peaks of B. dorsalis reasonably well, although agreement varied between sites. An index of agreement test indicated that GPFS model simulations matched with field B. dorsalis observation data with a range between 0.50 and 0.94 (1.0 as a perfect match). Of the three locations, Wuhan showed the highest match between the observed and simulated B. dorsalis populations, with indices of agreement of 0.85. The site-specific temporal comparisons implied that the GPFS model is informative for prediction of relative abundance. Spatial results from the GPFS model were also compared with 161 published observations of B. dorsalis distribution, mostly from East Asia. Since parameters for pupal overwintering and survival were unknown from the literature, these were inferred from the distribution data. The study showed that GPFS has promise for estimating suitable areas for B. dorsalis establishment and potentially other non-indigenous pests. It is concluded that calibrating prediction models with both spatial and site-specific temporal data may provide more robust and reliable results than validations with either data set alone.

An analytical model for the underwater sound pressure waveforms radiated when an offshore pile is driven.

An analytical model has been developed for the pile vibration and consequent sound pressure and particle velocity radiated underwater when an offshore cylindrical pile is struck by a drop hammer. The model, which is based on the coupled equations of motion for axial and radial vibration of a thin cylindrical shell, yields frequency-dependent phase velocity and attenuation of these vibrations. The amplitude of the pulse of axial and radial displacement that travels down a pile following an axial impact is described in terms of the hammer properties. Solutions are obtained for the radiated sound pressure and particle velocity, using Junger and Feit's Transform Formulation of the Pressure Field of Cylindrical Radiators [(Acoustical Society of America, New York, 1993), p. 216]. The model is applied to published data on radiated noise from offshore driving of a steel pile. The modeled pressure waveforms at 12-m horizontal range and at 9 hydrophone depths correlate significantly with the measured waveforms. The modeled pressures of the initial positive peaks (appropriately low-pass filtered) agree with data to within 1 dB. The initial negative peaks however exceed the data by up to 7 dB, and as hydrophone depth increases, the model negative peaks have a maximum at 7 m, whereas the data have a maximum at 9 m.

Properties of cervical and ocular vestibular evoked myogenic potentials (cVEMPs and oVEMPs) evoked by 500 Hz and 100 Hz bone vibration at the mastoid

ObjectiveTo define and compare the input–output properties of 500Hz and 100Hz bone-conducted (BC) transmastoid stimulation when evoking cVEMPs and oVEMPs. The findings for 500Hz were compared with those previously reported for air-conducted (AC) 500Hz stimulation. MethodsTwelve healthy subjects (18–57years old) participated in this study, with simultaneous recordings of cVEMPs and oVEMPs bilaterally. Initial intensities of 138dB (FL) were used and then reduced in 3 or 6dB increments to 105dB. ResultsFor BC 500Hz, like AC 500Hz, the relationship between the stimulus and reflex output fitted a power law for most peaks, but not the initial peak of the oVEMP. Some of the subjects did not have early crossed oVEMP responses for BC 500Hz but all did for 100Hz. For BC 100Hz, while the initial waveforms were similar, many of the peaks showed a tendency to saturate at the higher intensities. For both BC 500Hz and 100Hz, the initial (contralateral) oVEMP peaks had a higher threshold than for the (ipsilateral) cVEMP responses although this difference was more marked for 500Hz. ConclusionsBC 500Hz shows similar properties to AC 500Hz, consistent with an overlapping spectrum of afferents being excited by both, with bilateral effects for the BC stimulus. BC 100Hz evokes more non-vestibular peaks, presumably from muscle afferents, for the SCM recordings but is a more reliable method of evoking the early crossed potentials of the oVEMP. SignificanceBC 500Hz is a useful adjunct to the AC 500Hz stimulus and has similar properties. The BC 100Hz stimulus may be a more reliable method of evoking the early crossed oVEMP responses.

Atomistic investigation of the interfacial mechanical characteristics of carbon nanotube reinforced epoxy composite

In this work, we investigate the interfacial mechanical characteristics of carbon nanotube (CNT) reinforced epoxy composite using molecular dynamics (MD) simulations. The MD simulations were carried out by developing two pull-out models; namely, the displacement-load model and the velocity-load model. The second-generation force field – polymer consistent force field (PCFF) – is used in the current MD simulations. The effects of various parameters, such as epoxy density, length and diameter of a CNT, the CNT–epoxy interfacial thickness, LJ cut-off distance and capping conditions of a CNT on the interfacial mechanical characteristics have also been investigated and discussed. The results from the present pull-out model, which are validated with earlier studies, reveal that (i) the interfacial shear strength (ISS) of the CNT-reinforced epoxy composite is improved with the increase in the epoxy density, (ii) the ISS of the CNT-reinforced epoxy composite decreases with the increase in the values of length and diameter of a CNT, and the thickness of CNT–epoxy matrix interface, (iii) the LJ cut-off distance has marginal effect on the ISS and the pull-out force of CNT, and (iv) incorporation of an end cap in the simulations results high initial pull-out peaks, which well correlate with the experimental findings.

Tonal patterns, associations, and alignment of peaks in regional French

The intonation of regional varieties of French has been under-studied and remains an important empirical question. In this paper, I present an analysis of two varieties of French that are geographically distant but historically related. The main purpose of the analysis was to examine the effect of the regional factor on the tonal patterns, associations, and timing of the stress group initial and final peaks. The results showed no dialect effect on tonal pattern inventory and distribution or on tonal association and alignment. However, in both dialects women produced less utterance-medial falls and significantly delayed the peaks, compared with men. Additionally, the analysis suggested that the group initial versus final peaks in French appear to differ in secondary associations, which allows us to extend this concept to express the contrasts between different types of high targets and to detail the French language intonational grammar. This work contributes thus to the prosodic grammar of French and to the description of its dialects by providing evidence that they share the same intonational grammar but that groups of speakers demonstrate differences pertaining to its surface realization.

TRPV1 channels are involved in niacin-induced cutaneous vasodilation in mice.

Niacin is effective in treating dyslipidemias but causes cutaneous vasodilation or flushing, a side effect that limits its clinical use. Blocking prostaglandins in humans reduces but does not consistently eliminate flushing, indicating additional mechanisms may contribute to flushing. The transient receptor potential vanilloid 1 (TRPV1) channel, when activated, causes cutaneous vasodilation and undergoes tachyphylaxis similar to that seen with niacin. Using a murine model, early phase niacin-induced flushing was examined and TRPV1 channel involvement demonstrated using pharmacologic blockade, desensitization, and genetic knockouts (TRPV1 KO). The TRPV1 antagonist AMG9810 reduced the magnitude of the initial and secondary peaks and the rapidity of the vasodilatory response (slope). TRPV1 desensitization by chronic capsaicin reduced the initial peak and slope. TRPV1 KO mice had a lower initial peak, secondary peak, and slope compared with wild-type mice. Chronic niacin reduced the initial peak, secondary peak, and slope in wild-type mice but had no effect in knockout mice. Furthermore, chronic niacin diminished the response to capsaicin in wild-type mice. Overall, these data demonstrate an important role for TRPV1 channels in niacin-induced flushing, both in the acute response and with chronic administration. That niacin-induced flushing is a complex cascade of events, which should inform pharmacological intervention against this side effect.

Abstract 3695: The utility of plasma vascular endothelial growth factor and heparanase as pharmacodynamic markers following treatment with PG545, a heparan sulfate mimetic: preliminary evidence from preclinical and clinical settings

Abstract Background: The heparan sulfate mimetic PG545 has been demonstrated to inhibit the function of vascular endothelial growth factor (VEGF) and heparanase (HPSE) in vitro whilst downregulating HPSE expression in tumor and metastatic tissue in vivo. Although there are reports of consistent drug-induced increases in plasma VEGF with VEGF inhibitors such as bevacizumab, similar data are lacking for HS mimetics. Given PG545's mechanism of action which involves inhibition of both angiogenesis and metastasis, the evaluation of plasma VEGF and HPSE may represent pharmacodynamic (PD) biomarkers for each of these key processes. Materials and Methods: The levels of plasma VEGF and HPSE were measured using ELISA in samples from preclinical models of ovarian cancer (syngeneic ID8 and xenogeneic A2780 models) and a small cohort of patients (dosed with PG545 by the subcutaneous route) with advanced solid tumors from a Phase I clinical study. In tumor models, PG545 was administered once weekly by intravenous or intraperitoneal routes and efficacy was determined by quantifying tumor burden using the following methods: measurement of palpable solid tumors, abdominal circumference, ascites volume and/or by tracking cells using bioluminescent reporter imaging (depending on the model). In the A2780 model and the clinical samples, the pharmacokinetics of PG545 was also evaluated using LC-MS/MS, which ensured multiple samples to facilitate the measurement of VEGF and HPSE in plasma over the duration of the dosing interval following treatment with PG545. Results: PG545 demonstrated potent antitumor and antimetastatic activity when administered once weekly via intravenous or intraperitoneal routes in preclinical studies. These studies also indicated that PG545 treatment led to increases in plasma VEGF and HPSE. Importantly, plasma samples from advanced cancer patients treated with PG545 also revealed increases in these PD markers with initial peaks often correlating with Cmax of PG545 but longer-term gradual increases also noted in some instances. We consider that these increases in plasma levels result from PG545 binding to, thereby inhibiting, VEGF and HPSE in the tumor microenvironment. Conclusion: Given the questionable utility of single time point measures of plasma PD markers (including VEGF), these preliminary data suggest that kinetic profiling can reveal the previously undetermined dynamics of circulating VEGF and HPSE following treatment with PG545. Thus, the measurement of VEGF and HPSE appears to represent potential markers of pharmacodynamic activity. A new Phase I trial with intravenously-administered PG545 has recently commenced and should further clarify the potential utility of these basic PD biomarker responses in advanced cancer patients. Citation Format: Keith Dredge, Edward Hammond, Boris Winterhoff, Shailendra Giri, Attila Teoman, Darryn Bampton, Michael Millward, Viji Shridhar. The utility of plasma vascular endothelial growth factor and heparanase as pharmacodynamic markers following treatment with PG545, a heparan sulfate mimetic: preliminary evidence from preclinical and clinical settings. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 3695. doi:10.1158/1538-7445.AM2014-3695

Lightning attachment processes of an “anomalous” triggered lightning discharge

AbstractUsing a high‐speed optical imaging system specifically designed for observing the lightning attachment process, we have documented the process for stepped, dart, and dart‐stepped leaders in an anomalous rocket‐triggered lightning flash that terminated on a 10 m grounded utility pole. The initiation of the first return stroke was found to occur at a height of 23 ± 3 m above the top of the utility pole and was associated with three “slow front” dE/dt pulses. A time of 1.5 µs later, a fast rise in luminosity at 18 ± 2 m was associated with a “fast transition” dE/dt pulse. The first return stroke propagated bidirectionally from its initiation height, as did subsequent return strokes from their initiation heights of 8 ± 1 m to 16 ± 2 m above the top of the utility pole. The initial upward speed of the first return stroke was 1.4 × 108 m/s, while its initial downward speed was 2.2 × 107 m/s. The channel bottom luminosity of the first return stroke rose more slowly to a two or more times larger amplitude than that of the subsequent stroke luminosities. In contrast, the National Lightning Detection Network‐derived first‐return‐stroke peak current is smaller than that of the second and the third strokes, and our electric field records at 45 km show similar behavior for the initial field peaks of the first and subsequent strokes.

Ion-pair ultra-high performance liquid chromatographic analysis of monoamines: Peak-splitting at high flow rates

The use of ion-pair ultra-high performance liquid chromatography (UHPLC) coupled with electrochemical detection (ECD) is of great interest for the fast and sensitive determination of the monoamine neurotransmitters dopamine, noradrenaline and serotonin in microdialysis samples. However, when applying high flow rates in ion-pair UHPLC, other peaks than the initial compound peaks appear on the chromatogram. This peak-splitting phenomenon is caused by disturbed ion-pair retention mechanisms. The influence of several chromatographic parameters is investigated. Peak-splitting is delayed to higher flow rates when increasing the concentration of ion-pair reagent or buffering agent in the mobile phase, when decreasing the percentage of organic modifier in the mobile phase, when applying a stationary phase with a smaller amount of packing material or when increasing the separation temperature. One or a combination of these conditions can be applied to analyze the monoamine neurotransmitters using ion-pair UHPLC–ECD at high flow rates.

Low-dose nitroglycerine improves outcome after cardiac arrest in rats

ObjectiveThe aim of this study was to evaluate the outcome of intravenously applied nitroglycerine (NTG, 1μgkg−1min−1 for 1h) after resuscitation from an asphyxia cardiac arrest (ACA) insult. We hypothesized that NTG infused for 1h after the return of spontaneous circulation (ROSC) would improve functional and neuro-morphological outcomes. MethodsAdult rats were subjected to 8min of ACA followed by resuscitation. There were three treatment groups: ACA, ACA+NTG and sham operated. Vital and blood parameters were monitored during the 1h post-resuscitation intensive care phase. After survival times of 3, 6, 12, 24, 72h and 7 days, the neurological deficit score (NDS) was measured. Histological evaluation of the hippocampus, cortex, the thalamic reticular nucleus and the caudate-putamen was performed 7 days post insult. ResultsWe found that NTG (i) induced significantly higher initial MAP peaks; (ii) resulted in a less-pronounced elevation of heart rates after ROSC with significantly faster normalization to baseline levels; and (iii) influenced glucose metabolism, temporarily elevating blood glucose to non-physiological levels. Even so, NTG (iv) improved the neurological outcome and (v) reduced neurodegeneration, mainly in the hippocampal CA1 region. A significant NTG-associated decrease in blood pressure did not occur. ConclusionThe effect of low-dosed NTG applied post-resuscitation appears to be neuroprotective, demonstrated by reduced hippocampal damage and a better NDS, even with temporarily elevated blood glucose to non-physiological levels. Thus, additional studies are needed to evaluate NTG-triggered mechanisms and optimized dosages before clinical translation should be considered.Animal study institutional protocol number: 42502-2-2-947-Uni-MD.

Gamma-ray burst optical light-curve zoo: comparison with X-ray observations

We present a comprehensive analysis of the optical and X-ray light curves (LCs) and spectral energy distributions (SEDs) of a large sample of gamma-ray burst (GRB) afterglows to investigate the relationship between the optical and X-ray emission after the prompt phase. We collected the optical data from the literature and determined the shapes of the optical LCs. Then, using previously presented X-ray data we modeled the optical/X-ray SEDs. We studied the SED parameter distributions and compared the optical and X-ray LC slopes and shapes. The optical and X-ray spectra become softer as a function of time while the gas-to-dust ratios of GRBs are higher than the values calculated for the Milky Way and the Large and Magellanic Clouds. For 20% of the GRBs the difference between the optical and X-ray slopes is consistent with 0 or 1=4 within the uncertainties (we did it not consider the steep decay phase), while in the remaining 80% the optical and X-ray afterglows show significantly different temporal behaviors. Interestingly, we find an indication that the onset of the forward shock in the optical LCs (initial peaks or shallow phases) could be linked to the presence of the X-ray flares. Indeed, when X-ray flares are present during the steep decay, the optical LC initial peak or end plateau occurs during the steep decay; if instead the X-ray flares are absent or occur during the plateau, the optical initial peak or end plateau takes place during the X-ray plateau. The forward-shock model cannot explain all features of the optical (e.g. bumps, late re-brightenings) and X-ray (e.g. flares, plateaus) LCs. However, the synchrotron model is a viable mechanism for GRBs at late times. In particular, we found a relationship between the presence of the X-ray flares and the shape of the optical LC that indicates a link between the prompt emission and the optical afterglow.

Human occipital cortices differentially exert saccadic suppression: Intracranial recording in children

By repeating saccades unconsciously, humans explore the surrounding world every day. Saccades inevitably move external visual images across the retina at high velocity; nonetheless, healthy humans don't perceive transient blurring of the visual scene during saccades. This perceptual stability is referred to as saccadic suppression. Functional suppression is believed to take place transiently in the visual systems, but it remains unknown how commonly or differentially the human occipital lobe activities are suppressed at the large-scale cortical network level. We determined the spatial–temporal dynamics of intracranially-recorded gamma activity at 80–150Hz around spontaneous saccades under no-task conditions during wakefulness and those in darkness during REM sleep. Regardless of wakefulness or REM sleep, a small degree of attenuation of gamma activity was noted in the occipital regions during saccades, most extensively in the polar and least in the medial portions. Longer saccades were associated with more intense gamma-attenuation. Gamma-attenuation was subsequently followed by gamma-augmentation most extensively involving the medial and least involving the polar occipital region. Such gamma-augmentation was more intense during wakefulness and temporally locked to the offset of saccades. The polarities of initial peaks of perisaccadic event-related potentials (ERPs) were frequently positive in the medial and negative in the polar occipital regions. The present study, for the first time, provided the electrophysiological evidence that human occipital cortices differentially exert perisaccadic modulation. Transiently suppressed sensitivity of the primary visual cortex in the polar region may be an important neural basis for saccadic suppression. Presence of occipital gamma-attenuation even during REM sleep suggests that saccadic suppression might be exerted even without external visual inputs. The primary visual cortex in the medial region, compared to the polar region, may be more sensitive to an upcoming visual scene provided at the offset of each saccade.