A new calculation method of critical wind speed based on three degrees of freedom (3-DOF) is proposed for galloping problem of iced transmission line. Based on the quasistatic theory, the aerodynamic load of iced transmission line is obtained, which considers the influence of transverse and torsional motion on the relative wind angle of attack. Finally, the equivalent galloping model of 3-DOF iced transmission line is established. At the initial angle of attack, the aerodynamic load is expanded by Taylor, and the unsymmetrical linear aerodynamic coefficient matrix is obtained. The Routh–Hurwitz criterion is used to judge the stability of iced transmission line system, and then the critical wind speed is calculated. The in-plane and out-plane frequencies corresponding to the first-order mode of the transmission line are solved by the analytical method and numerical simulation method. The results obtained by the two methods are compared and verified. The influence of dimensionless transmission line parameterλon the in-plane and out-of-plane frequencies is discussed. The aerodynamic coefficients of the iced transmission line are measured by wind tunnel test and the aerodynamic characteristics are analyzed. According to the theoretical formula, the critical wind speed is calculated by MATLAB. The critical wind speed determined in this paper is compared with the critical wind speed determined by Den Hartog and Nigol theory. The influences of torsional vibration frequency, ice thickness, and ice shape on critical wind speed are analyzed. The research results of this paper have important theoretical significance for the stability judgment of iced transmission lines.