Inhibition Of Plaques Research Articles

Studies on the relationship between adeno-associated virus type 1 (AAV-1) and adenoviruses: II. Inhibition of adenovirus plaques by AAV; its nature and specificity

Plaque formation by simian adenoviruses, SV15 and SV34, and a simian papovavirus, SV40, was prevented in cell cultures treated with AAV. Specific antiserum to AAV inhibited this effect and thus provides a quantitative means for measuring AAV neutralizing antibody. The nature of the adenovirus inhibition does not suggest an interferon-like mechanism, but suggests an interference mediated directly by the AAV particle. This phenomenon, of adenovirus plaque inhibition, has been utilized in an attempt to determine the minimum number of interfering AAV particles present in a sample.