Background: Ginsenosides are known to have anti-cancer, anti-inflammatory, and antioxidant effects. Additionally, they are known to improve cognitive ability and memory, and prevent dementia. In this study, the physiological activity of cultured wild ginseng adventitious root (ginseng root, GS) containing low molecular weight ginsenosides was investigated in vitro.<BR>Methods and Results: This experiment was conducted to confirm the acetylcolinesterase (AChE) inhibitory activity, neuroprotectivie effect, and antioxidant effect of GS steam treatment. Through the steaming process, a GS steam treatment containing low molecular weight ginsenosides was prepared and evaluated. The results showed that AChE inhibitory activity, neuroprotective effect, reactive oxygen species (ROS) inhibitory effect, and catalase (CAT) activity effect by H₂O₂ were concentration-dependent in the group treated with GS steam treatment compared to those in the control group.<BR>Conclusions: In this study, it was demonstrated that the GS steam treatment influenced the AChE inhibitory activity, the neuroprotective effect, and the oxidative stress protective effect of GS.