Inhibition Capability Research Articles

NIR -I and NIR-II irradiation tumor ablation using NbS2 nanosheets as the photothermal agent.

Photothermal therapy has been considered a powerful means of cancer therapy due to its minimal invasiveness, effectiveness, and convenience. Although promising, the therapeutic effects are greatly limited as they rely on the photothermal agent (PTA). It is urgent to develop new PTAs with high photothermal conversion performance, especially under irradiation in the long-wavelength biowindows. Herein, a dual-biowindow-responsive PTA made of NbS2-PVP nanosheets was fabricated to be used both in the first near-infrared (NIR-I) and the second near-infrared (NIR-II) biowindows. With excellent hydrophilicity and biocompatibility, the nanosheets could effectively convert the near-infrared (NIR) light into heat, showing prominent photothermal stability. The calculated photothermal conversion efficiencies reached 59.2% (under NIR-I excitation) and 69.1% (under NIR-II excitation), respectively, which are comparable to those of metallic PTAs. The NbS2-PVP nanosheets had low cytotoxicity and could trigger strong photothermal treatment and cause cancer cell death upon irradiation by NIR-I or NIR-II light in vitro. Moreover, we have also demonstrated the highly efficient tissue ablation and tumor inhibition capability of NbS2-PVP nanosheets in vivo. This work explores an effective PTA of two-dimensional nanomaterials in NIR-I and NIR-II biowindows and offers a reference for the design of new kinds of PTAs.

UTILIZATION OF BETEL LEAVES EXTRACT TO PREVENT THE GROWTH OF Fusarium oxysporum f.sp. capsici CAUSING FUSARIUM WILT DISEASE IN BELL PEPPER

Betel (Piper betle Linn.) is commonly used in Indonesian traditional medicine due to its antimicrobial properties, thus it is often used as an ingredient in natural pesticides. The effectiveness of betel leaf extract as a control for Fusarium oxysporum in several plants showed it’s potential to control the fungi in bell pepper. F. oxysporum f.sp. capsici is one of the important pathogens causing Fusarium wilt disease in bell pepper. It is one of the most devastating plant diseases due to its ability to cause a crop failure. The test on the inhibitory capacity of betel leaf extract on the growth of F. oxysporum f.sp. capsici were carried out by growing the fungi on PDA mixed with various concentrations of betel leaf extracts. The results showed that betel leaf extract treatment at 0.02% concentration was able to inhibit the growth of F. oxysporum f.sp. capsici in PDA at 95.54%. The full inhibition of colony growth (100%) was achieved in the extract treated with the concentration of 0.03%. Meanwhile treatment of 0.17% betel leaf extract was able to inhibit the growth of F. oxysporum f.sp. capsici on bell pepper stems. Following this result, fractionation of the betel leaf extract by column chromatography was conducted, resulting in 44 fractions. The bioassays of those fractions showed that, there were seven fractions that reveal inhibition capability against F. oxysporum f.sp. capsici.

Aminated Fullerene Abrogates Cancer Cell Migration by Directly Targeting Myosin Heavy Chain 9.

Functional fullerene derivatives exhibit fantastic inhibitory capabilities against cancer survival and metastasis, but the absence of clarified biological molecular targets and ambiguous regulation mechanisms set barriers for their clinical transformation. Cancer metastasis is the primary cause of mortality and initiated with increased cell migration, making cell motility regulation a high-value therapeutic target in precision medicine. Herein, a critical molecular target of the aminated fullerene derivative (C70-EDA), myosin heavy chain 9 (MYH9), was initially identified by a pull-down assay and MS screening. MYH9 is a cytoplasm-located protein and is responsible for cell motility and epithelial-mesenchymal transition regulation. Omics data from large-scale clinical samples reveals that MYH9 gets overexpressed in various cancers and correlates with unfavorable prognosis, indicating that it is a potential antineoplastic target. It is unveiled that C70-EDA binds to the C-terminal of MYH9, triggering the transport of MYH9 from the cytoplasm to the cell edge, blocking the MYH9-involved cell mobility, and inhibiting the metastasis-associated EMT process. This work provides a precise biological target and new strategies for fullerene applications in cancer therapy.

Nanocatalytic Medicine of Iron-Based Nanocatalysts

Iron can be found in all mammalian cells and is of critical significance to diverse cellular activities within human bodies. Widespread applications and the underlying chemical and biological funda...

Antioxidant, Antibacterial and Anti-Diabetic Activities of Stingless Bee Honey from Selected Areas in Peninsular Malaysia

Stingless bee honey is one of the Malaysian honeys that has many benefits especially medically, due to the presence of enormous active phytochemical compounds. The active compounds in the honey vary depending on its geographical origin, especially the environmental conditions of the plant nectar. This study aims to analyze and compare the antioxidant, antibacterial and antidiabetic activities of stingless bee honey from different areas in Malaysia. The sample stingless bees’ honeys were obtained from Kulim, Kedah (honey sample 1), Tanjung Malim, Perak (honey sample 2) and Kuala Selangor, Selangor (honey sample 3). The methods used for the analysis were DPPH free radical scavenging, Kirby-Bauer disc diffusion assay and alpha-amylase inhibition assay for the respective activities. An analysis of gallic acid content of the stingless bee’s honeys were also performed using high-performance-liquid-chromatography (HPLC). Tukey’s multiple comparison test was used for statistical analysis. The results indicated that honey sample 2 exhibited the highest antioxidant activity with IC50 values of 89.04 ± 0.83 mg/mL, whilst honey sample 3 showed the highest inhibition capability on both E. coli and S. aureus at 16.33 ± 3.06 mm and 22.67 ± 0.58 mm respectively as compared to the other honey samples. Honey sample 3 also showed the highest inhibition against alpha amylase with an IC50 value of at 15.80 ug/mL. The gallic acid content of honey sample 3 also the highest at 39.79 ug/mL. As a conclusion, honey sample 3 originated from Kuala Selangor, Selangor has better antibacterial and anti-alpha amylase activities. Meanwhile, honey sample 2 originated from Tanjung Malim, Perak has better antioxidant properties.

Antilisterial Activity of Bacteriocins Produced by Lactic Bacteria Isolated from Dairy Products.

Sixty-nine Lactic Acid Bacteria (LAB) and bifidobacteria were isolated and identified from Italian dairy products (raw milk, cream, butter, soft cheese and yoghurt) to find new antimicrobial compounds to use as food bio-preservatives. All the isolates were preliminarily screened by the deferred antagonism method for bacteriocin production. Afterwards, to evaluate the release of bacteriocin in liquid medium, the Cell-Free Supernatant Fluid (CFSF) of the best producers was tested by agar well diffusion assay. The study allowed the selection of three bacteriocin producing strains (Enterococcus faecium E23, Bifidobacterium thermophilum B23 and Lactobacillus bulgaricus L21), endowed with the strongest and broadest inhibitory capability against the pathogen Listeria monocytogenes. The molecular characteristics and the chemical–physical properties of both producers and the respective bacteriocins were studied and compared. The results showed that E. faecium E23 was the best producer strain and its class IIa bacteriocins, called enterocin E23, exhibited a good spectrum of activity towards L. monocytogenes. Enterocin E23 was stable over a wide range of pH and at low temperatures for at least four months and, for this reason, it can be employed in refrigerated foods for the control of L. monocytogenes, the major concern in dairy products.

Emerging chemical scaffolds with potential SHP2 phosphatase inhibitory capabilities - A comprehensive review.

The drug discovery panorama is cluttered with promising therapeutic targets that have been deserted because of inadequate authentication and screening failures. Molecular targets formerly tagged as "undruggable" are nowadays being more cautiously cross-examined, and whilst they stay intriguing, numerous targets are emerging more accessible. Protein tyrosine phosphatases (PTPs) excellently exemplifies a class of molecular targets that have transpired as druggable, with several small molecules and antibodies recently turned available for further development. In this respect, SHP2, a PTP, has emerged as one of the potential targets in the current pharmacological research, particularly for cancer, due to its critical role in various signalling pathways. Recently, few molecules with excellent potency have entered clinical trials, but none could reach the clinic. Consequently, search for novel, non-toxic, and specific SHP2 inhibitors are on purview. In this review, general aspects of SHP2 including its structure and mechanistic role in carcinogenesis have been presented. It also sheds light on the development of novel molecular architectures belonging to diverse chemical classes that have been proposed as SHP2-specific inhibitors along with their structure-activity relationships (SARs), stemming from chemical, mechanism-based and computer-aided studies reported since January 2015 to July 2020 (excluding patents), focusing on their potency and selectivity. The encyclopedic facts and discussions presented herein will hopefully facilitate researchers to design new ligands with better efficacy and selectivity against SHP2.

Corrosion protection of petroleum oil well/tubing steel using thiadiazolines as efficient corrosion inhibitor: Experimental and theoretical investigation

Present investigation measures corrosion inhibition capabilities of thiadiazoline compounds; DFAT [2-4(difuran-2-yl-3-azabicyclo[3.3.1] nonan-9-yl-)-5-spiro-4-acetyl-2-acetylamino-1,3,4-thiadiazoline] and DIAT [2-4(di-1H indole-3-yl-3-azabicyclo[3.3.1]nonan-9-yl-)-5-spiro-4-acetyl-2-acetylamino-1,3,4-thiadiazoline] for petroleum oil well/tubing steel (N80 steel) in 15% HCl with the help of different techniques. The studied organic compounds show excellent corrosion mitigation properties with highest efficiency of 96.02% for DFAT and 97.05% for DIAT at a very small dosage of 50 ppm and 303 K temperature. Adsorption of both inhibitor molecules on the surface of N80 steel obeyed Langmuir adsorption isotherm. Potentiodynamic polarization studies revealed that both the compounds act as mixed type corrosion inhibitor and adsorb at the cathodic as well as anodic sites to mitigate corrosion process. The surface study was done through SEM, EDX, AFM, FT-IR and XPS analysis. The theoretical analysis (DFT and Monte Carlo simulations) of both inhibitors correlates the experimental findings.

Copper(II)-disulfiram loaded melanin-dots for cancer theranostics

Copper(II) diethyldithiocarbamate complex (CuET), the metabolite of disulfiram complexed with copper, is the component responsible for cancer treatment efficacy of disulfiram. But the hydrophobic property of CuET limits its use in vivo, and an appropriate drug delivery system needs to be developed. Ultrasmall melanin nanoparticle (M-Dot) with excellent biosafety and biocompatibility properties has been synthesized in our previous studies. Herein we prepared CuET loaded with M-Dots through hydrophobic interaction, which could enhance the water solubility significantly. After the administration of M-Dots-CuET in mice tumor models, the nanoparticles showed good tumor accumulation as evidenced by the enhanced photoacoustic signal in tumor regions. M-Dots-CuET also displayed excellent tumor inhibition capability, and the tumor growth inhibition value (TGI) was 45.1%. When combined with photothermal therapy, the TGI reached up to 78.6%. In summary, M-Dots-CuET provide a new potential strategy for cancer theranostics.

Mitigation of corrosion in petroleum oil well/tubing steel using pyrimidines as efficient corrosion inhibitor: Experimental and theoretical investigation

Present investigation focused on to study the corrosion inhibition capabilities of pyrimidine derivatives; MPA [4,6-bis(4-methoxyphenyl) pyrimidin-2-amine] and CPA [4,6-bis(4-chlorophenyl) pyrimidin-2-amine] for oil well/tubing steel (N80 steel) in 15 % HCl solution. Corrosion inhibition efficiency of both inhibitors increases on increasing the concentration and decreases on increasing the temperature. The studied organic compounds show excellent corrosion mitigation properties with highest efficiency of 99.5 % for MPA and 94.7 % for CPA at a considerably low dosage of 250 μM L−1. Adsorption of both inhibitors obeyed Langmuir adsorption isotherm and nature of adsorption is mixed type adsorption. Potentiodynamic polarization studies showed that both the compounds adsorb at the cathodic and anodic sites of the substrate surface to mitigate corrosion process and both inhibitors act as mixed type corrosion inhibitor. The surface study uninhibited and inhibited sample was done through SEM, EDX, AFM, FT-IR and XPS analysis and suggested the formation of protective layer of inhibitor molecules on the N80 steel surface to mitigate its corrosion. The theoretical analysis (DFT and Monte Carlo simulations) of both inhibitors correlates the experimental findings.

Development of β-carboline-benzothiazole hybrids via carboxamide formation as cytotoxic agents: DNA intercalative topoisomerase IIα inhibition and apoptosis induction

In quest of promising anticancer agents, the pharmacophores of natural (β-carboline) and synthetic origin (benzothiazole) were adjoined by a carboxamide bridge and three-point diversification was accomplished. The in vitro cytotoxic ability of the compounds was established on adherent and suspension human cancer cell lines and compounds 8u and 8f advanced as pre-eminent molecules with IC50 values of 1.46 and 1.81 μM respectively in A549 cell line. The cytospecificity was entrenched for potent compounds 8u and 8f by evaluating against normal human lung epithelial cells and selectivity index was calculated. Furthermore, EtBr displacement, relative viscosity and gel-based topoisomerase II target assays unveiled the intercalative topo-II inhibitory capability and DNA binding studies (absorbance) revealed the dissociation constant (Kd) for compounds 8u and 8f as 98 and 103 μM respectively. Additionally, cell-based flow cytometric assays like Annexin-V/PI dual staining aids in the quantification of apoptosis induced and JC-1 staining disclosed the depolarization of mitochondrial membrane potential by compound 8u in A549 cells in a dose-dependent manner. Moreover, wound healing assay established the inhibition of in vitro cell migration by compound 8u on A549 cells. In addition, molecular docking studies proved the binding of compounds 8u and 8f in the active site of DNA complexed with topo IIα and stabilized by interactions with DNA base pairs and amino acid residues. Remarkably, the compounds 8u and 8f follow Lipinski’s rule of five and are in the recommended range for Jorgensen’s rule of three with a minimal violation and other pharmacokinetic parameters revealing druggability of the synthesized hybrids.

Carrier-Free Platinum Nanomedicine for Targeted Cancer Therapy.

Numerous nanomedicines have been developed to improve the efficiency and safety of conventional anticancer drugs; however, the complexities in carrier materials and functional integration make it challenging to promote these candidates for clinical translation. In this study, a facile method to prepare carrier-free anticancer nanodrug with inherent bone targeting and osteoclastogenesis inhibition capabilities is reported. Phytic acid, a naturally occurring and nontoxic product, is reacted with cisplatin to form uniform nanoparticles of different sizes. The prepared nanoparticles possess high drug loading and pH-responsive drug release behaviors. Phytic acid in the nanomedicine ensures high bone targeting and osteoclastogenesis inhibition, and the released platinum drugs triggered by tumor extracellular acidity eradicate tumor cells. The nanomedicine around 100nm shows high anticancer activity and much reduced side effects in a subcutaneous breast cancer model when compared with cisplatin. In addition, it shows high accumulation at osteolytic lesions, and efficiently inhibits tumor growth and tumor-associated osteolysis in a bone metastatic breast cancer model. Here, a facile and efficient strategy to prepare carrier-free nanomedicines with high anticancer drug loading, inherent bone targeting, and osteoclast inhibitory activities for cancer therapy is provided.

Driving Towards Highly Selective and Coking‐Resistant Natural Gas Reforming Through a Hybrid Oxygen Carrier Design

AbstractCarbon deposition can be promoted by catalyst‐assisted C−H bond dissociation, which is one of the most concerning issues in reaction engineering. Treatment of carbon contamination inevitably generates CO2 which has a detrimental effect on the environment. Consequently, the development of efficient oxygen carriers is important to commercial viability of chemical looping processes. In this work, density functional theory (DFT) calculations were conducted and reveal that carbon deposition is a cascade reaction of accumulative C−C bond forming that deactivates LFO surface due to gradual accumulation of lattice oxygen vacancies. Guided by DFT mechanistic predictions, we tailor catalytic reactive perovskite LaFeO3 (LFO) with high oxygen carrying hematite Fe2O3 (FO) into a hybrid oxygen carrier LFO‐FO. The LFO‐FO oxygen carrier exhibits excellent carbon inhibition capability and high reactivity with syngas selectivity above 98 %. This work proposes a promising strategy toward oxygen carrier development with low cost, high reactivity, and selectivity for chemical looping technology.

PDE5 inhibition improves cardiac morphology and function in SHR by reducing NHE1 activity: Repurposing Sildenafil for the treatment of hypertensive cardiac hypertrophy

Previously, we have shown that an increased cGMP-activated protein Kinase (PKG) activity after phosphodiesterase 5 (PDE5) inhibition by Sildenafil (SIL), leads to myocardial Na+/H+ exchanger (NHE1) inhibition preserving its basal homeostatic function. Since NHE1 is hyperactive in the hypertrophied myocardium of spontaneous hypertensive rats (SHR), while its inhibition was shown to prevent and revert this pathology, the current study was aimed to evaluate the potential antihypertrophic effect of SIL on adult SHR myocardium. We initially tested the inhibitory capability of SIL on NHE1 in isolated cardiomyocytes of SHR by comparing H+ efflux during the recovery from an acid load. After confirmed that effect, eight-month-old SHR were chronically treated for one month with SIL through drinking water. Compared to their littermate controls, SIL-treated rats presented a decreased NHE1 activity, which correlated with a reduction in its phosphorylation level assigned to activation of a PKG-p38 MAP kinase-PP2A signaling pathway. Moreover, treated animals showed a decreased oxidative stress that appears to be a consequence of a decreased mitochondrial NHE1 phosphorylation. Treated SHR showed a significant reduction in the pro-hypertrophic phosphatase calcineurin, despite slight tendency to decrease hypertrophy was detected. When SIL treatment was prolonged to three months, a significant decrease in myocardial hypertrophy and interstitial fibrosis that correlated with a lower myocardial stiffness was observed. In conclusion, the current study provides evidence concerning the ability of SIL to revert established cardiac hypertrophy in SHR, a clinically relevant animal model that resembles human essential hypertension.

Carbon dots-releasing hydrogels with antibacterial activity, high biocompatibility, and fluorescence performance as candidate materials for wound healing

Antibacterial hydrogels have received attention for preventing infections and for their biomedical applications. However, traditional antibiotics-containing and metal nanoparticle-containing hydrogels often cause bacterial resistance, exhibit low biocompatibility, and lack real-time monitoring capability. Here, a fluorescent antibacterial hydrogel with antibacterial ability, excellent optical performance, and high biocompatibility was developed based on cationic carbon dots (CDs), pectin, and acrylic acid triggered construction of the hydrogel network by cross-linker. The antibacterial high-cationic CDs (+51.20 mV) were synthesized by a simple hydrothermal method and released from hydrogel in response to broken hydrogen bonds due to a change in the ambient environment caused by the growing bacteria. The hydrogel showed long-term potent broad-spectrum antibacterial ability (even drug-resistant bacteria) due to the bacterial membrane seriously damaged by the released CDs. The inhibitory capability of this hydrogel was 108.5-fold higher than the other hydrogel. After implantation or incubation with cells, no obvious cytotoxicity or tissue toxicity was observed for the antibacterial hydrogel. This hydrogel enhanced both the application of CDs in vivo and the biosafety of hydrogel. Furthermore, the multicolor fluorescence emission produced by CD provides a potential idea for the development of dual-function hydrogels with in situ monitoring and prevention of bacterial infections to treat wounds.

Terminal deoxynucleotidyl transferase combined CRISPR-Cas12a amplification strategy for ultrasensitive detection of uracil-DNA glycosylase with zero background

A simple and highly sensitive biosensing strategy was reported by cascading terminal deoxynucleotidyl transferase (TdT)-catalyzed substrate extension and CRISPR-Cas12a -catalyzed short-stranded DNA probe cleavage. Such a strategy, which is named as TdT-combined CRISPR-Cas12a amplification, gives excellent signal amplification capability due to the synergy of two amplification steps, and thus shows great promise in the design of various biosensors. Based on this strategy, two representative biosensors were developed by simply adjusting the DNA substrate design. High signal amplification efficiency and nearly zero background endowed the biosensors with extraordinary high sensitivity. By utilizing these two biosensors, ultrasensitive detection of uracil-DNA glycosylase (UDG) and T4 polynucleotide kinase (T4 PNK) was achieved with the detection limit as low as 5 × 10−6 U/mL and 1 × 10−4 U/mL, respectively. The proposed UDG-sensing platform was also demonstrated to work well for the UDG activity detection in cancer cells as well as UDG screening and inhibitory capability evaluation, thus showing a great potential in clinical diagnosis and biomedical research.

Potential of a volatile-producing endophytic fungus Nodulisporium sp. PDL-005 for the control of Penicillium digitatum

A volatile-producing endophytic fungus, PDL-005, with bio-fumigation activity was isolated from leaves of the medicinal plant Peperomia dindygulensis. PDL-005 showed an inhibitory efficacy or fungicidal ability against Penicillium digitatum Cmep1-3. The PDL-005 endophyte was identified as Nodulisporium sp. based on the morphology and ITS nucleotides. Testing different ingredients revealed that bagasse was the best material to produce active compounds, which exhibited good efficacy against the test pathogen. GC-MS analysis revealed that 2-ethyl-2-hexenal and 2,4-dimethyl-1,3-cyclopentanedione is the primary compound contributing to the inhibitory capability on radial growth of the pathogen. Moreover, PDL-005 cultured in bagasse reduced green mold incidences on orange markedly, sometimes up to a 100.0% reduction. Thus, PDL-005 could be potentially used as a bioagent to control postharvest disease caused by P. digitatum.

Polyethylene-Based Knee Spacer for Infection Control: Design Concept and Pre-Clinical In Vitro Validations.

Antibiotic-loaded polymethyl methacrylate (PMMA) has been widely applied in the treatment of knee periprosthetic joint infections. However, problems with antibiotic-loaded PMMA-based spacers, such as structural fracture and implant dislocation, remain unresolved. A novel polyethylene-based spacer, designed with an ultra-congruent articulating surface and multiple fenestrations, was introduced in the current study. Validation tests for biomechanical safety, wear performance, and efficacy of antibiotic cement were reported. During cycle fatigue testing, no tibial spacer failures were observed, and less wear debris generation was reported compared to commercial PMMA-based spacers. The volumetric wear of the novel spacer was within the safety threshold for osteolysis-free volumetric wear. An effective infection control was demonstrated despite the application of lesser antibiotic cement in the 30-day antibiotic elution test. The tube dilution test confirmed adequate inhibitory capabilities against pathogens with the loaded antibiotic option utilized in the current study. The novel polyethylene-based knee spacer may offer sufficient biomechanical safety and serve as an adequate carrier of antibiotic-loaded cement for infection control. Further clinical trials shall be conducted for more comprehensive validation of the novel spacer for practical application.

Ultrafiltration isolation, physicochemical characterization, and antidiabetic activities analysis of polysaccharides from green tea, oolong tea, and black tea.

Both fermentation degree and preparation method of polysaccharides could influence the bioactivity of tea polysaccharides. The aim of this study was to compare the physicochemical characterization and biological activities of the polysaccharides isolated by ultrafiltration method from three kinds of tea (green tea, oolong tea, and black tea). The bioactivities of tea polysaccharide fractions were compared from four aspects, including antioxidant activities, antiglycation activities, α-glucosidase inhibitory capability, and hypoglycemic effects on L6 myotubes. Results showed that six polysaccharides (GTPS1, GTPS2, OTPS1, OTPS2, BTPS1, and BTPS2) had different contents of neutral sugar and uronic acid, and they showed different morphologies. Six polysaccharides were composed of the seven monosaccharides with different molar ratios. BTPS1 exhibited the highest DPPH scavenging activity and hydroxyl radical scavenging activity (P < 0.05), and BTPS1 also showed the strongest antiglycation inhibitory effects (P < 0.05). BTPS1 and BTPS2 showed strong inhibitory capacity on α-glucosidase and hypoglycemic effects in L6 skeletal muscle cells. The result suggested that the degree of fermentation of tea could improve their bioactivities (BTPS > OTPS >GTPS), and TPS1 with smaller molecular weight distribution showed higher bioactivities than TPS2. This study can provide a scientific foundation for the application of tea polysaccharides and related functional products.

Biophysical Elucidation of Fibrillation Inhibition by Sugar Osmolytes in α-Lactalbumin: Multispectroscopic and Molecular Docking Approaches.

Protein aggregation is among the most challenging new frontiers in protein chemistry as well as in molecular medicine and has direct implications in protein misfolding. This study investigated the role of sugar molecules (glucose, fructose, sucrose, and the mixture of glucose and fructose) in protecting the structural integrity of α-lactalbumin (α-LA) against aggregation. The research focused here is the inhibitory capabilities of sugars against α-LA fibril formation investigated employing diverse multispectroscopic and microscopic techniques. The aggregation was induced in α-LA thermally with a change in concentration. UV–vis spectroscopy, ThT binding assay, Trp fluorescence, Rayleigh scattering, and turbidity assay depicted synchronized results. Further, transmission electron microscopy (TEM) complemented that a mixture of glucose and fructose was the best inhibitor of α-LA fibril formation. Inhibition of α-LA aggregation by sugar osmolytes is attributed to the formation of hydrogen bonds between these osmolytes, as evidenced by the molecular docking results. This hydrogen bonding is a key player that prevents aggregation in α-LA in the presence of sugar osmolytes. This study provides an insight into the ability of naturally occurring sugar osmolytes to inhibit fibril formation and can serve as a platform to treat protein misfolding and aggregation-oriented disorders.