Inhalation Of Dust Research Articles

Use of soybean as an alternative protein source for welfare-orientated production of American alligators (Alligator mississippiensis).

Soybean meal based diets have been suggested to cause gastrointestinal issues in certain species when used as a protein alternative. Using a randomized design, we tested 1,728 alligators in one of 13 communal pens offered one of two diets (seven pens (n = 928) of soybean-based protein diets and six pens (n = 800) of animal-based protein diets) to determine if soybeans would negatively affect the growth, hide quality, behavior and health, when compared with an otherwise identical diet using animal-based protein. Both feeds were nearly identical in composition for protein and fat percentages and identical for all minerals and elements. Crude protein was a minimum of 50%, crude fat a minimum of 12%, crude fiber a minimum of 4%, and phosphorous was maintained at 1%. From this information we estimated the One Welfare of using soy as a protein in commercial diets. Although there was a statistically significant decrease in belly width (p = 0.0009; harvested hide size) for alligators fed soybean-based protein diets, all other measured parameters of soybean vs. animal-based protein diets were comparable, suggesting this environmentally sustainable alternative protein source warrants consideration as a feed base. Weight was not significantly different suggesting either diet would yield similar volumes of meat. Total length was significantly affected by diet. Hide quality was not negatively impacted by protein type, with both diets producing high quality hides free of defects (assessed at the salted hide stage prior to tanning). Behaviors were not influenced by the feed type, with animals fed either diet using the pen structures the same. Further, feeding times were the same suggesting the soybean-based protein diet was equally easy to eat and palatable as the animal-based protein diet. Behavior and feeding suggested soy-based diets do not alter time budgets or activities. There were no differences in the frequency or severity of pathologies for animals fed either diet. Respiratory (lung and trachea as a proxy to measure dust inhalation), gastrointestinal (small intestine as a proxy to measure digestive disturbances), and renal (kidney as a proxy for excretory stress) histopathology demonstrated neither diet was causing overt problems. One Welfare conclusions were feeding a soybean-based protein diet did not cause production or welfare issues. Further, soybean protein-based diets may be an environmentally sustainable alternative to currently used animal-based diets. Research examining different soybean protein concentrations and sources is warranted.

Effects of Cement Dusts on Peak Expiratory Flow Rate (PEFR) in Female Cement Dust Exposed Construction Workers

Background: Occupational and environmental lung diseases are the major problems of clinical medicine. The cement dust workers are constantly exposed to high amounts of visible ambient air particulate matter, mostly without any respiratory protective device. Therefore, the inhalation of dust over periods of time leads to impaired lung functions. Objectives: To observe the effects of cement dust exposure on PEFR in female cement dust workers. Methods: This cross-sectional study was conducted in the Department of Physiology, Rangpur Medical College, Rangpur from July 2016 to June 2017. A total of 60 female subjects aged 20–50 years were selected for the study. Among them, 30 were apparently healthy workers exposed to cement dust, and 30 apparently healthy height, weight & BMI-matched subjects not exposed to cement dust were selected as control from the surrounding community. Their pulmonary functions were studied by measuring PEFR. For statistical analysis, an unpaired ‘t’-test was performed by computer-based software SPSS-17.0 version for Windows. Results: The mean measured and mean percentage of the predicted value of PEFR was significantly (p_0.001) lower in cement dust-exposed female workers (CD- EFW) than the non-exposed female workers (CD-NEFW). Conclusion: From the result of this study, it can be concluded that cement dust (CD) may have harmful effects on some pulmonary functions. J Rang Med Col. September 2023; Vol. 8, No. 2: 25-28

Bacterial contamination of coffee and personal exposure to inhalable dust and endotoxin in primary coffee processing factories in Ethiopia.

Endotoxins from gram-negative bacteria might be released when the coffee cherries are processed and may cause respiratory health problems among workers in the coffee industry. The relationship between bacterial contamination and occupational exposure to endotoxin levels has not been thoroughly explored previously in primary coffee processing factories in Ethiopia, or elsewhere. The aim of this study was to characterize the level of personal endotoxin exposure and its relations with bacterial contamination of coffee cherries in such factories in Ethiopia. A cross-sectional study was conducted from March 2020 - February 2021 in 9 primary coffee processing factories in 3 regions in Ethiopia. A total of 180 personal air samples were collected to analyze workers' exposure to inhalable dust and endotoxin. Correlation tests were performed to assess the relationship between total bacteria and endotoxin levels and between inhalable dust and endotoxin levels. The geometric mean (GM) of personal inhalable dust exposure among machine room workers and hand pickers were 9.58mg/m3 and 2.56mg/m3, respectively. The overall GM of endotoxin exposure among machine room workers and hand pickers were 10,198 EU/m3 and 780 EU/m3, respectively. Gram-negative bacteria were found in all 54 coffee samples. The correlation between inhalable dust and endotoxin exposure was significant (r=0.80; P <0.01). About 92% of the samples from hand pickers and all samples from machine room workers exceeded the occupational exposure limit of 90 EU/m3 recommended by the Dutch Expert Committee on Occupational Standards. Prevention and control of bacterial contamination of the coffee in primary coffee processing are suggested to reduce endotoxin exposure that might cause respiratory health problems among coffee workers.

Risk Analysis of Occupational Health and Safety (OHS) The Construction of the Road in Dumai City (Case Study: Sp. Batang – Sp. Kulim, Sp. Batang – Sp. Terminal and Bts. City of Dumai – Duri)

The rapid development of the construction industry brings not only benefits but also risks. This study will analyze the risks and risk controls that occur in the management of occupational safety and health of road construction projects in the City of Dumai. The method used in this study is qualitative by using a decision tree classification algorithm. The results showed that the main determining variable for the frequency of work accidents on the Dumai City road project was getting hit by a mobilized dump truck, while other risks were traffic jams, being hit by a vibro roller, workers' skin exposed to hazardous materials, and inhalation of hazardous material dust with the results of predictive accuracy and the results of the reality on the ground, namely the frequency of work accidents that occurred in the Dumai city road project, namely 66.67%. The risk control is based on the element requirements of the OHSAS 18001: 2007 Occupational Safety Management System, namely substitution, engineering, administrative controls, and personal protective equipment (PPE).

Laboratory study of the effectiveness of substituting traditional wheat flour with low dust flour and use of different sieve designs as controls to reduce exposure to inhalable flour dust in commercial bakeries.

Exposure to flour dust remains one of the leading causes of occupational asthma in Great Britain (GB). The average annual incidence rate per 100,000 bakers and flour confectioners in GB was 47.8 for the 3 yr period 2017 to 2019 compared with 0.53 for all occupations. There are many processes in commercial bakeries that can cause exposure to flour dust. Exposures are typically controlled by using local exhaust ventilation or respiratory protective equipment. The aim of this study was to evaluate the potential to reduce exposure to inhalable flour dust in commercial bakeries by modification of the process by use of a conical sieve in place of a round sieve; and substitution of traditional wheat flour (TWF) with 'low dust' flours LD1 and LD2 for dusting surfaces. Two simulated commercial bakery tasks were performed in a laboratory whilst dust exposures were measured in the breathing zone of the operator using an Institute of Occupational Medicine (IOM) sampler, button sampler and a real-time direct reading monitor. Analysis of variance tests were used to assess whether differences in mean exposures were statistically significant with the different control approaches. A qualitative visual exposure assessment was also undertaken using Tyndall illumination. Substituting TWF with LD1 and LD2 reduced exposure to inhalable flour dust by 86% and 53% respectively when sieving and by 78% and 67% respectively when filling a hopper. There was no statistically significant difference between dust emissions for all 3 flours when using the conical sieve instead of the round sieve for flour dusting tasks. This laboratory study has shown that substituting TWF with low-dust flour may reduce inhalable dust exposures when dusting surfaces in bakeries.

Health Risk Assessment of Inhalable Dust Exposure during the Welding and Grinding Process of Subway Aluminum Alloy Components

The subway factory industry is developing rapidly in China, but there are some occupational health risk assessments of inhalable dust in this industry. Therefore, this study aimed to explore the contamination level and health risks of dust in an aluminum alloy body workshop of subway factories in Hunan Province, China. A total of 160 dust samples were collected from the welding and grinding areas. The main elements of PM10 were analyzed. The health risks of aluminum dust and PM2.5 were evaluated. The Monte Carlo method was adopted to compare the sensitivity of the Hazard Quota (HQ) of aluminum dust and carcinogenic risk (CR) of PM2.5 to the exposure parameters for workers. The results showed that the PM10 concentration in the grinding area was higher, while the PM2.5 concentration in the welding area was higher. The metal element with a mass fraction of 27.7% was aluminum. In both areas, the probability of the aluminum dust HQ exceeding 1 was approximately 17% and 68%, respectively. The PM2.5 CR exceeded the acceptable upper limit value (1.0 × 10−4). The main risk factor of aluminum dust HQ was concentration, while the main risk factors of PM2.5 CR were concentration and exposure duration. These findings provide basic data for enhancing health risk management in the subway industry.

Assessment of exposure to fungi in archives and libraries based on analyses of filter and nasal samples: preliminary investigation

The aim of this study was to characterize the occupational exposure to inhalable dust and airborne fungi among archive and library workers based on qualitative and quantitative analyses of stationary and personal filter samples as well as nasal swabs. The study was carried out in 3 archives and 2 libraries and involved 9 workers of these institutions. Airborne fungi and inhalable dust samples were collected by stationary and personal measurements using filter samplers. Additionally, the nasal swabs from workers were taken after work-shift and microbiologically analysed. The average concentrations of inhalable dust and airborne fungi were 49 µg/m3 (SD = 91) and 299 CFU/m3 (SD = 579), respectively. Both dust and bioaerosol concentrations obtained using personal measurements were significantly higher than that measured by stationary sampling. The correlation analysis showed strong relationships between the concentrations of inhalable dust and airborne fungi (R = 0.57; p < 0.001). The fungal concentrations in swab samples from archive workers (median: 104 CFU/ml) were significantly higher than that in swabs from librarians (median: 1.4 CFU/ml). Among the airborne fungi, the widest spectrum of species was found among Penicillium and Aspergillus (including pathogenic A. fumigatus) genera. However, in samples from archives, yeast-like fungi from Sporidiobolus and Candida (including pathogenic C. albicans) genera predominated among isolated mycobiota. The results of this study revealed that airborne fungi were able to efficiently contaminate the nasal cavity of archive and library employees. The analysis of nasal swabs can be considered as an important analytical tool supporting the assessment of workers’ exposure to bioaerosols.

Hazardous Impact of Coal Dust on Hematological Parameters of Underground Coal Mine Workers

Due to the nature of their work, coal miners have historically faced significant exposure to large amounts of dust, placing them at a heightened risk. The present study was conducted to explore the hazardous effects of inhalation of coal dust on the haematological parameters of underground coal miners. 120 underground coal miners (60 workers having 5-10 years of experience and the other 60 having 10-20 years of experience) and 62 matched non-exposed to coal dust subjects as control from the locality were recruited for the study. After obtaining consent, different physiological parameters were measured, and blood was collected for assessment of haematological parameters. Observations revealed that there were lower mean values of total RBC count, haemoglobin level, MCV, PCV, MCHC and MCH in coal mine workers whereas mean values of Red Cell Distribution Width (RDW) and ESR were increased in the workers group. Further, coal dust exposure causes increased total leucocyte to count as well as a differential count of lymphocyte, neutrophil, monocyte, and eosinophil in the workers group but, a decreased differential count of basophils was detected among coal dust-exposed workers. All the changes in haematological parameters were found to occur experience-dependent and maximum changes were observed in workers having 10-20 years of working experience. There is a significant likelihood of coal mine workers experiencing changes in their blood composition, which suggests the harmful impact of coal dust on this group. Further, haematological parameters will help health professionals to screen any pathologic conditions and may help to prevent coal dust exposure-associated haematological disorders and complications thereof.

Assessment of working conditions and health conditions of employees of non-ferrous metallurgy enterprises

The production conditions of non-ferrous metallurgy enterprises can be characterized by a high severity of the labor process due to excessive physical exertion, constant exposure to intense noise, increased vibration levels; inhalation of industrial aerosols, dust and chemicals; exposure to unfavorable industrial microclimate. The most common types of professionally conditioned and occupational pathology in employees of non-ferrous metallurgy departments are sensorineural hearing loss, vibration disease, radiculopathy, lung and upper respiratory tract diseases, diseases of the musculoskeletal system.&#x0D; In order to summarize the information and identify the peculiarities of working conditions and health status of employees of non-ferrous metallurgy enterprises, the researchers analyzed the relevant scientific literature on the bibliographic databases Scopus, Web of Science, MedLine and RSCI using theoretical methods of scientific cognition.&#x0D; The most intense impact of production factors is characterized by the working conditions of workers of underground divisions of non-ferrous metallurgy enterprises.&#x0D; At the same time, the presence of a specific chemical factor associated with exposure to dust and gas mixtures and aerosols of non-ferrous metals causes the development of morbidity in workers of metallurgical industries for processing and enriching raw materials of non-ferrous metals with toxic alveolitis, bronchial asthma, malignant neoplasms, diseases of the skin and subcutaneous tissue, blood and central nervous system. In this regard, at non-ferrous metallurgy enterprises, when planning a set of preventive measures, the identification of groups of diseases associated with working conditions and caused by the presence of a specific chemical factor of exposure to dust and gas mixtures and aerosols of non-ferrous metals should be considered a necessary condition for preserving the health of workers.&#x0D; Ethics. This study did not require the conclusion of the ethics committee.

Lifestyle features as co-factors in head and neck cancer development

Ranked sixth most prevalent worldwide, head and neck cancers (HNCs) a􀵵ect an increasing number of people. Several factors contribute to their occurrence, including genetic and epigenetic aberrations and lifestyle factors, including heavy smoking, alcohol consumption, chewing betel quid (Areca nuts), poor oral hygiene, consumption of pro-in􀃀ammatory foods, inhalation of chemical compounds, asbestos dust, and infections with HPV (human papillomavirus) and EBV (Epstein-Barr virus). Often diagnosed in advanced stages, they have a mortality rate of about 50% at 5 years and pose a serious threat to human health.

Effect of Hardwood Dust and Ammonia Gas on the Respiratory Integrity of Broiler Chickens.

Dust and ammonia gas (NH3) are two of the most abundant pollutants suspended in the air of poultry houses. Chronic inhalation of poultry dust and NH3 causes damage to the airways and reduces performance in broilers. Poultry dust is a mixture of organic and inorganic matter from feed, bedding material, manure, feathers, skin debris, and microorganisms. Thus, the composition and concentration of poultry dust vary among farms. This study proposes a model to assess the individual effect of a defined fraction of poultry dust derived from bedding material (wood dust) and its effects, alone or combined with NH3, on the performance and respiratory integrity of broilers. Ninety-six, 1-day-old broilers were randomly divided into groups of 24 and placed into four controlled environment chambers to continuously receive one of four treatments: 1) negative control; 2) exposure to airborne red oak wood dust at a concentration of 7.5 × 106 particles/m3 (particulate matter5.0); 3) exposure to 50 parts per million (ppm) of NH3; and 4) exposure to airborne red oak wood dust and 50 ppm of NH3. On day 43, all birds were weighed and euthanized. Performance data were recorded. Tissue samples were collected from six birds per treatment. Histologic evaluations of the nasal turbinates, trachea, and lungs were conducted. Histologic lesion scores (0 to 3) were assigned, and tracheal mucosal thickness was measured. No significant differences among treatments were found in body weight (P = 0.066), tracheal mucosal thickness (P = 0.593), or tracheal lesion score (P = 0.07). The average nasal turbinate lesion scores were higher in the wood and wood + ammonia treatments compared with the control (P = 0.015). The lung lesion scores were higher (P = 0.004) in all treatment groups compared with the control. In conclusion, chronic exposure to red oak wood dust, alone or in combination with NH3, induced important inflammatory damage to portions of the respiratory system of broilers; however, no significant effects on performance were observed.

Cement dust inhalation induces hepato-renal dysfunction via tissue heavy metal bioaccumulation, histopathological and biochemical mechanisms

Cement dust inhalation induces hepato-renal dysfunction via tissue heavy metal bioaccumulation, histopathological and biochemical mechanisms

Frequent Allergic Sensitization to Farmed Edible Insects in Exposed Employees

Exposure to insects used in pet food, scientific research, or live fish bait can cause an occupational allergy. The recent shift toward enhanced insect production for human consumption and animal feed will likely expose more employees. To investigate sensitization and symptoms in employees exposed to edible insects in Flanders. Fifteen insect-exposed employees were recruited and sensitization was explored by skin prick test, basophil activation test, and immunoblotting. Lung function, FeNO, histamine provocation, and sputum induction were studied. Airborne dust sampling was performed and proteins were studied by silver stain and immunoblotting. Sixty percent of employees self-reported upper respiratory tract symptoms related to insect exposure. Ten employees (71.4%) had a positive histamine provocation test concentration causing a 20% drop in FEV1 less than 8 mg/mL and four (26.7%) had FeNO levels above 25 ppb. Four employees (30.7%) had a positive skin prick test for at least one insect, and seven (58.3%) had a positive basophil activation test. In eight participants with insect sensitization, four (50%) had co-occurring house dust mite sensitization. Two participants had strong IgE binding to a 50-kDa migratory locust allergen, one to a 25-kDa mealworm allergen, and one to mealworm α-amylase. In one center, facility adjustment resulted in a substantial decrease in the inhalable dust fraction. Insect exposure leads to high levels of sensitization among employees. Most employees reported symptoms of the upper respiratory system, and two-thirds of employees had bronchial hyperreactivity. Prevention and health surveillance will be important in the developing insect-rearing industry.

Nine human epidemiological studies on synthetic amorphous silica and respiratory health

The respiratory health effects of Synthetic Amorphous Silica (SAS) have been studied in human epidemiological research. This article presents a historical overview and review of nine occupational worker studies that have been conducted so far on this topic. The combined study population of all of these studies included 1172 employees, and exposure concentrations ranged from < 1 mg/m3 to 100 mg/m3. In two studies with a total of 293 workers, the incidence of silicosis was investigated after long-term exposure to precipitated SAS, and no cases of silicosis were found (Plunkett and Dewitt, 1962; Volk, 1960). In another study, the spirometry results of 40 workers were normal (Vitums et al., 1977). In a study of 28 workers, 4 cases of silicosis were identified, but it is possible that contamination with cristobalite occurred and detailed information about the amorphous silica origin was not provided (Mohrmann and Kahn, 1985). Ferch et al. (1987) found that lung impairment was associated with confounding factors (smoking) but not with exposure to precipitated SAS in a study of 143 workers. Choudat et al. (1990) reported a reduction in forced expiratory flow in a group exposed to precipitated SAS compared to a control group. Still, they found no correlation between the extent of exposure and pulmonary function was found in a study of 131 workers. Wilson et al. (1979) also failed to show a significant association between the degree of exposure to precipitated SAS and annual changes in lung function in a study of 165 workers. In the most recent and most extensive study (Taeger et al., 2016; Yong et al., 2022) in Germany, involving 462 factory workers, no association between inhalable or respirable SAS dust exposure and respiratory health was reported. Based on the available data, there is no evidence-base to support a relationship between SAS and respiratory health in humans.

Occupational health: normative view for the diagnosis of silicosis

Silicosis is an occupational lung fibrosis caused by the inhalation of dust containing free crystalline silica. Its prevalence can vary in different countries and regions. In the case of Chile, silicosis has been recognized as a significant occupational health problem. The clinical presentation of silicosis largely depends on the intensity and duration of exposure. Initially, there may be no signs or symptoms. The differential diagnosis is mainly performed with pulmonary tuberculosis, pulmonary aspergillosis, pulmonary metastasis, sarcoidosis, hypersensitivity pneumonitis, and other pneumoconiosis. After confirming the diagnosis, the process of determining the origin of the pathology begins, for which an Occupational History is required to describe occupational exposure to the risk agent (silica) in terms of time, magnitude, and/or intensity sufficient to develop an occupational-related disease. A Chest X-ray using the ILO (International Labour Organization) technique is necessary, classified as category 1 according to the ILO Classification, and should be interpreted by certified readers. The similarity in radiographic readings necessitates a greater emphasis on the differential diagnosis among these conditions. A high- resolution chest computed tomography without contrast and a lung biopsy should be considered in case of comorbidity, if these diagnostic tests are available. Finally, it is suggested that workers diagnosed with silicosis in the early stages should be identified and removed from silica exposure to prevent disability through medical surveillance.

Assessment of respiratory health status of workers in flour mills of Assiut

BackgroundWorking environment should not present a risk of injury or disease but many thousands of workers worldwide remain exposed to hazardous substances particularly in developing countries. Flour dust is one of those hazardous substances to which the flour mills workers are exposed to, inhalation of flour dust may cause diverse lung diseases with different severity of symptoms ranging from simple irritation to allergic reaction and chronic respiratory disorders, including asthma. Therefore, the present study was done to detect the prevalence of respiratory problems among flour mills workers.MethodsThe study was carried out in the flour mills of Assiut, it was a cross-sectional study among 203 workers. The study instruments were a semi-structured questionnaire about respiratory symptoms, chest examination and pulmonary function tests (PFT).ResultsThe age of the study workers ranged from 19 to 60 years old, 94.1% were males, 50.7% were from urban regions. Pulmonary function abnormalities were found in 36.4% of them and 20.7% were diagnosed with bronchial asthma. Significant relationships were detected between abnormal pulmonary function tests and workers ≥ 40 years old, low educational level and those who worked in the flour mills for ≥ 10 years.ConclusionsFlour dust cause respiratory symptoms and impair the pulmonary function of the flour mills workers and they may develop bronchial asthma which affect their work efficiency.RecommendationsMedical care must be provided regularly to the flour mills workers and safety measures are mandatory.Trial registrationClinicalTrials.gov. NCT03678519. Registered August 10, 2022.

Construction 3D-printing in reducing the incidence of long latency respiratory diseases among construction workers in the UK

Onsite construction workers are exposed to many hazards which affect their body. However, dust and silica dust inhalation are often overlooked, most likely because it takes years to identify the side effects. The damage inflicted to the lungs is often irreversible as it is often discovered when it is too late. The aim of this research is to address the problem of occupational respiratory diseases among construction workers and investigate the potential of construction 3D printing in reducing the incidence of some of these diseases. The research objectives were to: identify the causes of the most prevalent respiratory diseases in construction; investigate the strategies of minimising onsite dust/ silica dust exposure; investigate the benefits of 3D printing in the minimisation of onsite dust/ silica dust exposure; explore the barriers of 3D printing in the minimisation of onsite dust/ silica dust exposure and explore the strategies for wider adoption of construction 3D printing to minimise the incidence of long latency respiratory diseases among construction workers. To achieve these objectives, a literature review was conducted, an online survey was carried out and professionals and researchers in the 3D printing field were interviewed to obtain relevant information to understand the intricacies of the new technology and its impact from workers’ health perspectives.

Evaluation of immunotoxicity of sodium metavanadate following drinking water exposure in female B6C3F1/N mice in a 28-day study.

Sodium metavanadate (NaVO3 ) is a pentavalent vanadium compound used in the metal industry and dietary supplements; human exposure occurs through inhalation of fumes and dust and ingestion of NaVO3 -containing products. The objective of this study was to assess the potential immunotoxicity of NaVO3 . Female B6C3F1/N mice were exposed to 0-500 ppm NaVO3 in drinking water for 28 days and evaluated for effects on immune cell populations and innate, cellular-mediated, and humoral-mediated immunity. There was a decreasing trend in body weight (BW) and BW gain in NaVO3 exposed mice, with a decrease (p ≤ 0.05) in BW gain at ≥250 ppm, relative to control. Conversely, increasing trends in spleen weights and an increase (p ≤ 0.05) in the spleen:BW ratio at ≥250 ppm NaVO3 were observed. NaVO3 exposure altered antibody production against sheep red blood cells (SRBC). Antibody forming cells (AFC)/106 spleen cells exhibited a decreasing trend, with a decrease (p ≤ 0.05) at 500 ppm NaVO3 , concurrent with an increase in percent B cells. NaVO3 had no effect on the serum anti-SRBC IgM antibody titers or anti-keyhole limpet hemocyanin antibody production. Exposure to NaVO3 decreased the percentage of natural killer cells at all dose levels (p ≤ 0.05), with no effect on the lytic activity. NaVO3 altered T-cell populations at 500 ppm but had no effect on T-cell proliferative responses or the lytic activity of cytotoxic T cells. Collectively, these data indicate that NaVO3 exposure can adversely affect the immune system by inducing alterations in humoral-mediated immunity, specifically the AFC response, with no effect on cell-mediated or innate immunity.

Purification and characterization of a serine protease from organic dust and elucidation of its inductive effects on lung inflammatory mediators.

Organic dust inhalation is associated with the development of respiratory diseases. Serine protease activities in organic dusts were previously reported to contribute to the induction of lung inflammatory mediators however, the identities of the proteases and the mechanisms by which they induce inflammatory mediators are unknown. The goal of this study was to purify and characterize serine protease(s) from organic dust and elucidate mechanisms by which they induce lung inflammatory mediators. A serine protease was purified from poultry organic dust by benzamidine-agarose affinity chromatography. Mass spectrometry and amino-terminal sequence analysis identified the purified protease as chicken trypsin II-P29. Purified protease induced proinflammatory cytokine levels in Beas2B and NHBE epithelial and THP-1 macrophage cells. Treatment with the purified protease increased cellular and mitochondrial reactive oxygen species (ROS) generation. Induction of inflammatory mediators and ROS were suppressed by serine protease inhibitors and antioxidants. Purified protease activated protein kinase C (PKC), mitogen-activated protein kinase (MAPK)1/3 and MAPK14 signaling, and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and signal transducer and activator of transcription 3 (Stat-3), and chemical inhibitors targeting these pathways suppressed induction of inflammatory mediators. Calcium mobilization studies showed that the purified protease activated protease-activated receptors (PAR) F2R, F2RL1, F2RL2, F2RL3, and F2R and F2RL1 knockdown suppressed the induction of inflammatory mediators. Intranasal instillation of purified protease increased lung chemokine (C-X-C motif) ligand (CXCL)1, interleukin (IL)-6, and tumor necrosis factor (TNF) levels in mice. Our studies have shown that chicken trypsin is a proinflammatory constituent of poultry organic dust, and induces lung inflammatory mediators via increased ROS and PAR activation in a cell signaling pathway involving PKC, MAPK1/3 and MAPK14, and NF-κB and Stat-3.NEW & NOTEWORTHY Inhalation of dust in industrial agricultural operations is linked to the development of lung diseases. Our studies have isolated for the first time a trypsin protease from poultry farm dust and have shown that it stimulates lung inflammation. The protease stimulates the production of oxidants and cell signaling pathways to increase inflammatory mediator production. Targeting trypsin protease in poultry farm environment may be a useful strategy to counter the harmful effects of dust.

Analisis Identifikasi Bahaya K3 dengan Metode HIRARC pada Unit Recycle Industri Tekstil Nonwoven

Occupational Safety and Health (K3) is a program created by employees and employers as an effort to prevent work-related accidents and diseases by identifying things that have the potential to cause work-related accidents and diseases as well as anticipatory actions in the event of work-related accidents and diseases. The method used in this study is the HIRARC (Hazard Identification, Risk Assessment, and Risk Control) method. This method consists of a series of OSH implementations which include hazard identification, risk assessment, and determination of control measures based on the data collected. The results of the risk assessment are used as a reference in the implementation of risk control. This study uses surveys and interviews to collect data to identify potential hazards in the recycle unit, risk assessment and risk control are carried out by observing and identifying as well as interviewing workers and company owners in the recycle unit. From the results of the identification of the recycle unit that has been carried out, there are several potential hazards that can be identified and the risk assessment and risk categories in the recycle unit can be identified. Based on the results of the study, there were 31 potential hazards in the recycle unit, of which 19.3% were in the low risk category, 61.2% were in the moderate risk category, and 16.6% were in the high risk category. Identification of hazards carried out in the work process in the recycle unit, potential hazards contained in the recycle unit, namely; exposed wires, inhalation of dust, exposure to noise, exposure to hot engines, and exposure to hot polymer spills.