Infrared Imaging Research Articles

GERB Obs4MIPs: a dataset for evaluating diurnal and monthly variations in top-of-atmosphere radiative fluxes in climate models

Abstract. A newly available radiative flux dataset specifically designed to enable the evaluation of the diurnal cycle in top-of-atmosphere (TOA) fluxes as captured by climate and Earth system models is presented. Observations over the period 2007–2012 made by the Geostationary Earth Radiation Budget (GERB) instrument are used to derive monthly hourly mean outgoing longwave radiation (OLR) and reflected shortwave (RSW) fluxes on a regular 1° latitude–longitude grid covering approximately 60° N–60° S and 60° E–60° W. The impact of missing data is evaluated in detail, and a data-filling solution is implemented using estimates of broadband fluxes from the Spinning Enhanced Visible and Infrared Imager flying on the same Meteosat platform, scaled to the GERB observations. This relatively simple approach is shown to deliver an approximate improvement by a factor of 10 in both the bias caused by missing data and the associated variability in the error. To demonstrate the utility of this V1.1 filled GERB Observations for Climate Model Intercomparison Projects (Obs4MIPs) dataset, comparisons are made to radiative fluxes from two climate configurations of the Hadley Centre's Global Environmental Model: HadGEM3-GC3.1 and HadGEM3-GC5.0. Focusing on marine stratocumulus and deep convective cloud regimes, diurnally resolved comparisons between the models and observations highlight discrepancies between the model configurations in terms of their ability to capture the diurnal amplitude and the phase in TOA fluxes, details that cannot be diagnosed by comparisons at lower temporal resolutions. For these cloud regimes the GC5.0 configuration shows improved fidelity to the observations relative to GC3.1, although notable differences remain. The V1.1 filled GERB Obs4MIPs monthly hourly TOA fluxes are available from the Centre for Environmental Data Analysis, with the OLR fluxes accessible at https://doi.org/10.5285/90148d9b1f1c40f1ac40152957e25467 (Bantges et al., 2023a) and the RSW fluxes accessible at https://doi.org/10.5285/57821b58804945deaf4cdde278563ec2 (Bantges et al., 2023b).

FCS-Net: Feather condition scoring of broilers based on dense feature fusion of RGB and thermal infrared images

Assessing the feather condition of broilers is crucial for monitoring the animal welfare status and detecting the occurrence of feather pecking activities. Currently, the feather condition of individual broilers is manually scored by trained experts. To provide a more objective and efficient tool for feather condition scoring, a novel deep learning-based model, named Feather Condition Scoring Network (FCS-Net), was proposed based on RGB and thermal infrared images. The FCS-Net model combined the ResNet18 architecture with the proposed Dense Feature Fusion (DFF) module, which can effectively learn the feature mapping relationship between RGB and thermal infrared images. Before inputting the images into the network, an image registration process was conducted to align the RGB and thermal infrared images. The results showed that the FCS-Net model had a good performance for feather condition scoring, with the Accuracy of 97.02%, the Precision of 96.99%, the Recall of 97.04%, the F1 of 97.01%, and the Inference speed of 15.34 fps. Compared to the ResNet18_RGB model, which only utilise RGB images, the FCS-Net model showed notable improvements in Accuracy by 4.02%, Precision by 3.90%, Recall by 4.08%, and F1 by 4.01%. Moreover, it was observed that the FCS-Net model focused more on the back region of the broilers through heatmap visualization. Furthermore, the algorithm was compared with six typical image recognition algorithms including VGG16, ResNet18, SE-ResNet18, DenseNet121, Mobilenet_V2, and Shufflenet_V2_x1_0, as well as the state-of-the-art (SOTA) feather condition assessment methods. The results showed that the FCS-Net model achieved better performance than the six algorithms and the SOTA feather condition assessment methods. This study provided a valuable reference for automated monitoring of feather condition scoring of broilers in smart farming.

Damage assessment in composite plates using extended non-destructive self-heating based vibrothermography technique

Self-heating based vibrothermography (SHVT) is a promising non-destructive technique for inspecting polymer-matrix composites (PMCs) in circumstances with limited external heating feasibility, functioning under the resonant frequency excitation. This study broadened the applicability of SHVT technique for two-dimensional (2D) PMCs by examining its sensitivity and effectiveness in detecting and quantifying damage within 2D laminated composites across nine different scenarios. Atwo-step algorithm based on the concepts of the boundary of effective thermograms, and the maximal temperature ratio was proposed to methodically choose the optimal raw thermogram from a large set of registered thermograms for each scenario. The issues linked to blurred damage signatures in the raw infrared (IR) images adversely affected the overall sensitivity and accuracy of SHVT. Implementing the algorithm based on central moments improved the overall damage detectability using this technique. Additionally, the introduced hit/miss metric enabled the damage identification and quantification for all scenarios.

Benchtop IR Imaging of Live Cells: Monitoring the Total Mass of Biomolecules in Single Cells.

Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of chemical quantification, its applicability to hydrated biological samples remains challenging due to the strong IR absorption by water. Traditional IR imaging of hydrated cells relies on powerful light sources, such as synchrotrons, to mitigate the light absorption by water. However, we overcome this challenge by applying a solvent absorption compensation (SAC) technique to a home-built benchtop IR microscope based on an external-cavity quantum cascade laser. SAC-IR microscopy adjusts the incident light using a pair of polarizers to precompensate the IR absorption by water while retaining the full dynamic range. Integrating the IR absorbance over a cell yields the total mass of biomolecules per cell. We monitor the total mass of the biomolecules of live fibroblast cells over 12 h, demonstrating promise for advancing our understanding of the biomolecular processes occurring in live cells on the single-cell level.

Fusing infrared polarization images for road detection via denoising diffusion probabilistic models.

Recent advancements in road detection using infrared polarization imaging have shown promising results. However, existing methods focus on refined network structures without effectively exploiting infrared polarization imaging mechanisms for enhanced detection. The scarcity of datasets also limits the performance of these methods. In this Letter, we present a denoising diffusion model aimed at improving the performance of road detection in infrared polarization images. This model achieves effective integration of infrared intensity and polarization information through forward and reverse diffusion processes. Furthermore, we propose what we believe to be a novel method to augment polarized images from different orientations based on the angle of polarization. The augmented polarized image serves as the guiding condition, enhancing the robustness of the diffusion model. Our experimental results validate the effectiveness of the proposed method, demonstrating competitive performance compared to state-of-the-art methods, even with fewer training samples.

Can Multiscale Thermal Infrared Imaging Help Validate and Monitor Water Stress in Alluvial Forests?

ABSTRACTAlluvial forests are sensitive to drought induced by climate change and exacerbated by altered flow regimes. Our ability to detect and map their sensitivity to drought is crucial to evaluate the effects of climate change and adjust management practices. Therefore, we explore the potential of multiscale thermal infrared imagery (TIR) to diagnose their sensitivity to droughts. In summer 2022, we sampled leaves and phloem on Populus nigra trees from two sites with contrasted hydrological connectivity along the Ain River (France) to investigate the seasonality of water stress and act as ground truth for airborne TIR images. To map forest sensitivity to drought, we used TIR data from four airborne campaigns and Landsat archives over a larger spatial and temporal extent. Field data showed that stress conditions were reached for both sites but were higher in the site with lower groundwater connectivity, which was also the case for individual tree crown temperatures. At the forest plot scale, canopy temperature was linked to forest connectivity for two of four TIR campaigns, with higher values in the more degraded reaches. Landsat data were used to locate the areas of the riparian forest impacted by a historical drought event and monitor their recovery and proved useful to identify trends. TIR data showed promising results to help detect and map tree water stress in riparian environments. However, stress is not detected in all TIR campaigns, demonstrating that one‐shot TIR acquisitions alone are not enough to diagnose stress and complementary in‐field eco‐physiological measurements are necessary.

Scalable Fabrication of Black Phosphorous Films for Infrared Photodetector Arrays.

Bulk black phosphorous (bP) exhibits excellent infrared (IR) optoelectronic properties, but most reported bP IR photodetectors are fabricated from single exfoliated flakes with lateral sizes of <100µm. Here, scalable thin films of bP suitable for IR photodetector arrays are realized through a tailored solution-deposition method. The properties of the bP film and their protective capping layers are optimized to fabricate bP IR photoconductors exhibiting specific detectivities up to 4.0×108cm Hz1/2 W-1 with fast 30/60 µs rise/fall times under λ = 2.2µm illumination. The scalability of the bP thin film fabrication is demonstrated by fabricating a linear array of 25 bP photodetectors and obtaining 25×25pixel IR images at ≈203ppi with good spatial fidelity. This research demonstrates a commercially viable method of fabricating scalable bP thin films for optoelectronic devices including room temperature-operable IR photodetector arrays.

Long-wavelength, high-resolution microscopy using upconversion in ultra-thin crystals

In this paper, a novel approach based on frequency upconversion in ultra-thin nonlinear crystals is investigated for use in high-resolution infrared (IR) microscopy in the 5–12 µm range, an important domain for biomedical research. Traditional IR imaging encounters spatial resolution constraints due to diffraction, which are addressed via upconversion imaging using ultra-thin crystals. The present work combines a tunable IR quantum cascade laser and a short wavelength mixing laser to circumvent the classical resolution limit dictated by the Rayleigh criterion. A detailed numerical model for small signal upconversion imaging at μm-scale resolution shows good agreement with experimental data. The presented approach opens new avenues for IR applications for label-free biomedical diagnostics and spectral imaging.

YOLO-SGF: Lightweight network for object detection in complex infrared images based on improved YOLOv8

The current mainstream object detection networks perform well in RGB visible images, but they require high computational resource and degrade in performance when applied to low-resolution infrared images. To address above issues, we propose a lightweight algorithm YOLO-SGF based on you-only-look-once version8 (YOLOv8). Firstly, the lightweight cross-scale feature map fusion network GCFVoV designed as neck to solve poor detection accuracy and maintain low complexity in lightweight networks. And a lightweight GCVF module in GCFVoV neck uses GSConv and Conv to process deep and shallow features respectively, which maximally preserves implicit connections between each channel and integrates multi-scale features. Secondly, we utilize ShuffleNetV2-block1 in combination with C2f for feature extraction, making the algorithm more lightweight and effectively. Finally, we propose the FIMPDIoU loss function, which focuses on overlooked objects in complex backgrounds and adjusts the prediction boxes using ratios specific to different sizes of objects. Compared with YOLOv8 in our infrared dataset, YOLO-SGF reduces the computational space complexity by 50 % and time complexity by 42 %, increases FPS32 by 36.3 % and improves mAP@0.5 ∼ 0.95 by 1.1 % in object detection. Our algorithm enhances the capability of object detection in infrared images especially in nighttime, low light, and occluded conditions. YOLO-SGF enables deployment on embedded edge devices with limited computing power, and provides a new idea for lightweight networks.

A model for energy predictions and diagnostics of large-scale photovoltaic systems based on electric data and thermal imaging of the PV fields

The aim of this investigation is the development of robust models for the performance prediction and automatic monitoring of large photovoltaic systems, based on historical and real-time electric and thermal data. This issue is increasingly important due to the worldwide diffusion of large photovoltaic systems and their need to identify and predict failures and malfunctions, in order to promptly assess the convenience of maintenance actions. The present model describes the response to irradiance and temperature conditions of both modules and inverters and also it is able to predict shading conditions able to affect the energy yield. The model has been validated against real electric measurements in 6 large PV plants located in southern Italy and it demonstrated to be able to predict the real time power production within a 4.1 % error. Even more importantly, the model and its comparison with subhourly measurements over several years has demonstrated its effectiveness in detecting downtime conditions caused by inverter or string problems. Simulations and measurements revealed that missed energy production due to electrical grid coupling downtime can exceed 50 % on certain days and that the shading conditions (up to 5 % of the daily energy production) can be easily detected and separated from component problems, thus avoiding false alarms. Finally, the analysis of aerial infrared images allowed to further test the model in failure detection capability, assess the relationship between thermal anomalies and underperformance conditions and in predicting the yearly deterioration rate at the PV plants.

Facile preparation of graphene aerogel as a thermal insulation material

A simple low temperature and cost-effective hydrothermal method via reduction self-assembly and freeze-drying was fabricated to prepare graphene aerogel (rGO). After the reduction process, most of the oxygen-containing groups in graphene oxide have been removed by ascorbic acid. The surface C/O atomic ratio detected in rGO is about 7.26. The aerogel presents a nanoporous structure. This porous structure could act as a barrier to heat conduction. By heating the graphene aerogel and using infrared thermal imager to detect the temperature, the temperature difference between the top and the bottom of the aerogel reached 80 °C, which showed excellent thermal insulation performance. In addition, by testing the thermal imaging image of graphene aerogel placed on the skin surface, it exhibited an infrared stealth effect. This carbon aerogel may offer potential for the fabrication and application for thermal insulation and infrared stealth material systems.

Tropical cyclone tracking from geostationary infrared satellite images using deep learning techniques

ABSTRACT Accurate tracking of tropical cyclones (TCs) can provide regions of interest for intelligent forecasting of TC tracks and intensity. There have been few studies on algorithms for automatic TC tracking. This study proposes an effective TC tracking method based on deep learning combined with infrared satellite images. The study first constructed a TC tracking dataset based on the infrared images of the China Fengyun-2D geostationary satellite covering six different TC intensity levels between 2009 and 2012. This included 47 complete cases (video sequences) of TCs from generation to extinction. Based on deep learning, the visual tracking algorithm SiamRPN was used as the model framework. Combining Bi-GRU and TC cloud spatiotemporal evolution characteristics to improve the performance of the SiamRPN network, the SiamTCNet target-tracking model was designed to track TCs automatically. Considering that the shape and scale of TC changes with time, a TC is regarded as a typical non-rigid object with obvious timing characteristics, so the first frame of a TC video sequence is combined with the satellite images of the first three frames of the current frame as inputs to the proposed SiamTCNet model, which then extracts the evolution of the TC’s spatial structure and its bidirectional temporal change information. The experimental results show that the TC tracking of the proposed model is a significant improvement over the original SiamRPN model.

WaveFusionNet: Infrared and visible image fusion based on multi-scale feature encoder–decoder and discrete wavelet decomposition

To merge complementary information from multimodal images, such as thermal saliency from infrared images and texture details from visible images, traditional multi-scale transform-based methods have been extensively studied, with deep learning-based methods gaining significant popularity in recent years. However, there has been limited research on optimally combining the advantages of these two categories in fusion. In this paper, we propose a novel infrared and visible image fusion (IVIF) framework, WaveFusionNet, which integrates precise frequency feature decomposition from the discrete wavelet transform (DWT) with the comprehensive feature extraction from the multi-scale encoder. Firstly, we train an encoder–decoder network for multi-scale feature extraction and image reconstruction. DWT is used for down-sampling with minimal information loss by decomposing extracted features into low and high-frequency sub-bands. Next, a dual-band feature fusion (DBFF) module is trained to merge these sub-bands by integrating a spatial feature transform-based sub-network for low-frequency fusion and a maximum absolute value selection strategy for fusing high-frequencies. Finally, all fused sub-bands are fed into the pre-trained decoder to reconstruct the final image. Experimental results on three benchmark datasets (TNO, Roadscene, and MSRS) demonstrate that the proposed fusion method outperforms recent IVIF methods in both quantitative assessment and visual perception while maintaining competitive time complexity.

Infrared Image Enhancement Method of Substation Equipment Based on Self-Attention Cycle Generative Adversarial Network (SA-CycleGAN)

During the acquisition of infrared images in substations, low-quality images with poor contrast, blurred details, and missing texture information frequently appear, which adversely affects subsequent advanced visual tasks. To address this issue, this paper proposes an infrared image enhancement algorithm for substation equipment based on a self-attention cycle generative adversarial network (SA-CycleGAN). The proposed algorithm incorporates a self-attention mechanism into the CycleGAN model’s transcoding network to improve the mapping ability of infrared image information, enhance image contrast, and reducing the number of model parameters. The addition of an efficient local attention mechanism (EAL) and a feature pyramid structure within the encoding network enhances the generator’s ability to extract features and texture information from small targets in infrared substation equipment images, effectively improving image details. In the discriminator part, the model’s performance is further enhanced by constructing a two-channel feature network. To accelerate the model’s convergence, the loss function of the original CycleGAN is optimized. Compared to several mainstream image enhancement algorithms, the proposed algorithm improves the quality of low-quality infrared images by an average of 10.91% in color degree, 18.89% in saturation, and 29.82% in feature similarity indices. Additionally, the number of parameters in the proposed algorithm is reduced by 37.89% compared to the original model. Finally, the effectiveness of the proposed method in improving recognition accuracy is validated by the Centernet target recognition algorithm.

Human sleeping pose estimation from IR images for in-bed patient monitoring using image processing and deep learning techniques

Human Pose Estimation (HPE) is a crucial step towards understanding people in images and videos. HPE provides geometric and motion information of the human body, which has been applied to a wide range of applications (e.g., human-computer interaction, motion analysis, augmented reality, virtual reality, healthcare, etc.). An extremely useful task of this kind is the 2D pose estimation of bedridden patients from infrared (IR) images. Here, the IR imaging modality is preferred due to privacy concerns and the need for monitoring both uncovered and covered patients at different levels of illumination. The major drawback of this research problem is the unavailability of covered examples, which are very costly to collect and time-consuming to label. In this work, a deep learning-based framework was developed for human sleeping pose estimation on covered images using only the uncovered training images. In the training scheme, two different image augmentation techniques, a statistical approach as well as a GAN-based approach, were explored for domain adaptation, where the statistical approach performed better. The accuracy of the model trained on the statistically augmented dataset was improved by 124 % as compared with the model trained on non-augmented images. To handle the scarcity of training infrared images, a transfer learning strategy was used by pre-training the model on an RGB pose estimation dataset, resulting in a further increment in accuracy of 4 %. Semi-supervised learning techniques, with a novel pose discriminator model in the loop, were adopted to utilize the unannotated training data, resulting in a further 3 % increase in accuracy. Thus, significant improvement has been shown in the case of 2D pose estimation from infrared images, with a comparatively small amount of annotated data and a large amount of unannotated data by using the proposed training pipeline powered by heavy augmentation.

Shortwave Infrared Imaging of a Quantum Dot-Based Magnetic Guidewire Toward Non-Fluoroscopic Peripheral Vascular Interventions.

Peripheral vascular interventions (PVIs) offer several benefits to patients with lower extremity arterial diseases, including reduced pain, simpler anesthesia, and shorter recovery time, compared to open surgery. However, to monitor the endovascular tools inside the body, PVIs are conducted under X-ray fluoroscopy, which poses serious long-term health risks to physicians and patients. Shortwave infrared (SWIR) imaging of quantum dots (QDs) has shown great potential in bioimaging due to the non-ionizing penetration of SWIR light through tissues. In this paper, a QD-based magnetic guidewire and its system is introduced that allows X-ray-free detection under SWIR imaging and precise steering via magnetic manipulation. The QD magnetic guidewire contains a flexible silicone tube encapsulating a QD polydimethylsiloxane (PDMS) composite, where HgCdSe/HgS/CdS/CdZnS/ZnS/SiO2 core/multi-shell QDs are dispersed in the PDMS matrix for SWIR imaging upon near-infrared excitation, as well as a permanent magnet for magnetic steering. The SWIR penetration of the QD magnetic guidewire is investigated within an artificial tissue model (1% Intralipid) and explore the potential for non-fluoroscopic PVIs within a vascular phantom model. The QD magnetic guidewire is biocompatible in its entirety, with excellent resistance to photobleaching and chemical alteration, which is a promising sign for its future clinical implementation.

Highly robust thermal infrared and visible image registration with canny and phase congruence detection

When a dual-optical vehicle-mounted camera is photographed, visible and thermal infrared (VI-T) images cannot be registered, and realising fine matching of the images to solve for the optimal affine parameters is thus key to this research. Visible and thermal image matching is of great interest owing to the presence of challenging scale transformations, nonlinear radial aberrations, and robustness under illumination conditions. Thus, this paper presents a novel algorithm, namely, Highly Robust Thermal Infrared and Visible Image Registration with Canny and Phase Congruence Detection (HRCP). Firstly, the image is pre-processed, and feature point detection is performed by combining adaptive thresholding GW-Canny edge detection and phase coherence (PC), which increases the edge information. Secondly, SURF and FAST feature detection at the maximum distance of PC maps can obtain stable and reliable feature points, which are robust to scale invariance and rotation invariance. Finally, the optimal affine parameter is solved using the least square method with several iterations. Compared to existing matching methods, we find that VI-T image matching is particularly sensitive to both lighting factors and scale-transformed nonlinear radial distortion and that HRCP is robust in performing matching. For the randomly selected dataset, the average image matching time is reduced by 3.265 s, the number of correct image matches increases by 44 pairs on average, the root mean square error improves to 1.830, and the average error improves to 2.815. The proposed method not only improves the inaccuracy of radiation-insensitive feature transform matching in both low and high light, but also achieves a higher accuracy and an increase in the number of feature matching points, which facilitates robust VI-T image registration.

Multi-scale feature extraction and fusion with attention interaction for RGB-T tracking

RGB-T single-object tracking aims to track objects utilizing both RGB images and thermal infrared(TIR) images. Though the siamese-based RGB-T tracker shows its advantage in tracking speed, its accuracy still cannot be compared with other state-of-the-art trackers (e.g., MDNet). In this study, we revisit the existing siamese-based RGB-T tracker and find that such fall behind comes from insufficient feature fusion between RGB image and TIR image, as well as incomplete interactions between template frame and search frame. Inspired by this, we propose a multi-scale feature extraction and fusion network with Temporal-Spatial Memory (MFATrack). Instead of fusing RGB image and TIR image with the single-scale feature map or only high-level features from the multi-scale feature map, MFATrack proposes a new fusion strategy by fusing features from all scales, which can capture contextual information in shallow layers and details in the deep layer. To learn the feature better for tracking tasks, MFATrack fuses the features via several consecutive frames. In addition, we also propose a self-attention interaction module specifically designed for the search frame, highlighting the features in the search frame that are relevant to the target and thus facilitating rapid convergence for target localization. Experimental results demonstrate the proposed MFATrack is not only fast, but also can obtain better tracking accuracy compared with other competing methods including MDNet-based methods and other siamese-based trackers.

Enhanced Detection and Recognition of Road Objects in Infrared Imaging Using Multi-Scale Self-Attention.

In infrared detection scenarios, detecting and recognizing low-contrast and small-sized targets has always been a challenge in the field of computer vision, particularly in complex road traffic environments. Traditional target detection methods usually perform poorly when processing infrared small targets, mainly due to their inability to effectively extract key features and the significant feature loss that occurs during feature transmission. To address these issues, this paper proposes a fast detection and recognition model based on a multi-scale self-attention mechanism, specifically for small road targets in infrared detection scenarios. We first introduce and improve the DyHead structure based on the YOLOv8 algorithm, which employs a multi-head self-attention mechanism to capture target features at various scales and enhance the model's perception of small targets. Additionally, to prevent information loss during the feature transmission process via the FPN structure in traditional YOLO algorithms, this paper introduces and enhances the Gather-and-Distribute Mechanism. By computing dependencies between features using self-attention, it reallocates attention weights in the feature maps to highlight important features and suppress irrelevant information. These improvements significantly enhance the model's capability to detect small targets. Moreover, to further increase detection speed, we pruned the network architecture to reduce computational complexity and parameter count, making the model suitable for real-time processing scenarios. Experiments on our self built infrared road traffic dataset (mainly including two types of targets: vehicles and people) show that compared with the baseline, our method achieves a 3.1% improvement in AP and a 2.5% increase in mAP on the VisDrone2019 dataset, showing significant enhancements in both detection accuracy and processing speed for small targets, with improved robustness and adaptability.

Are the Hypo-Reflective Clumps Associated With Age-Related Macular Degeneration in Adaptive Optics Ophthalmoscopy Autofluorescent?

Hypo-reflective clumps (HRCs) are structures associated with age-related macular degeneration (AMD) that were identified using flood-illumination adaptive optics ophthalmoscopy (FIAO) and hypothesized to be either macrophages that have accumulated melanin through the phagocytosis of retinal pigmented epithelial (RPE) cell organelles or transdifferentiated RPE cells. HRCs may be autofluorescent (AF) in the near infrared (NIR) but clinical NIR autofluorescence imaging lacks the resolution to answer this question definitively. Here, we used near infrared autofluorescence (NIRAF) imaging in fluorescence adaptive optics scanning laser ophthalmoscopy (AOSLO) to determine whether HRCs are AF. Patients with AMD and HRCs underwent imaging with FIAO, optical coherence tomography (OCT), and multi-modal AOSLO (confocal, NIRAF, and non-confocal multi-offset detection using a fiber bundle). HRCs were segmented on FIAO and images, co-registered across modalities, and HRC morphometry and AF were quantified. Eight patients participated (mean age = 79 years, standard deviation [SD] = 5.7, range = 69-89 years, and 5 female patients). Most HRCs (86%, n = 153/178) were autofluorescent on AOSLO. HRC AF signal varied but most uniformly dark HRCs on FIAO showed corresponding AF on AOSLO, whereas heterogeneous HRCs showed a smaller AF area or no AF. These findings are consistent with the hypothesis that HRCs contain AF RPE organelles. A small proportion of HRCs were not AF; these may represent macrophages that have not yet accumulated enough organelles to become AF. HRCs may have clinical significance but further study is needed to understand the interplay among HRCs, RPE cells, and macrophages, and their relationship to geographic atrophy (GA) progression in AMD.