INTRODUCTION: TikTok has emerged as a leading social media platform, with many users using the app for educational purposes, including for discussions about obstetrics and gynecology. We assessed tone, content, and quality of health information of TikTok videos tagged with #PapSmear. METHODS: One hundred videos tagged with #PapSmear on TikTok were collected using the data-scraping program, Apify (Prague, Czech Republic). Videos were extracted with their number of views, likes, comments, and shares, and analyzed for tone, themes, creator demographics and health information quality. Two modified standardized scales were used: DISCERN, for health information quality and Patient Education Materials Assessment Tool (PEMAT), for understandability and actionability. RESULTS: We reviewed 93 of 100 videos tagged with #PapSmear. Videos received 237 million views, 19 million likes, and 241 thousand comments in total. Videos were coded as positive (26.9%), negative (37.6%), and neutral (35.5%). Medical professionals created most of the positive videos (68.0%), whereas most negative video creators were not medical professionals (91.4%). Nearly half of positive videos were educational (48.0%), and most negative videos recounted personal patient experiences (60.0%). Videos scored high on understandability (average 73.4%, SD 16.3%) but low on actionability (average 31.9%, SD 34.1%) and quality of health information (2.12, SD 0.4). CONCLUSION: There is a discrepancy in tone between patients and medical professionals in videos tagged with #PapSmear. Videos with educational material were easy to understand, but provided low-quality health information and did not effectively highlight opportunities for patient action. Negative influence from TikTok should be considered in patient–provider communication about Pap smears.