Influence Feeding Behavior Research Articles

Thunderstorms have species and gear‐specific indirect effects on the catchability of Mongolian salmonids

AbstractClimate change is predicted to cause increasingly frequent and intense storms. Northern Mongolia is already warming at a rate twice the global average, and thunderstorms, defined as intense, short, patchy rains associated with thunder, lightning and high precipitation rates, are becoming more frequent. Because Mongolia's fish populations are lightly exploited, Mongolia provides a model system in which to study the effects of storms on fish behaviour and fishing vulnerability. The impacts of thunderstorm‐related hydrological changes on fishes’ vulnerability to two fishing gears were evaluated. Two thunderstorm‐related factors, turbidity and river stage, reduced catch rates of the salmonids lenok Brachymystax lenok (Pallas) and Baikal grayling Thymallus baicalensis Dybowski. Fly‐fishing gear was more effective than spinning gear in this fishery and retained higher catch rates in extreme conditions. These gear‐specific effects suggest that turbidity and rising river stage affect fishing vulnerability by influencing feeding behaviour.

Canine ocular onchocerciasis in New Mexico: Risk factors for disease.

To determine whether dogs are at variable risk of developing canine ocular onchocerciasis based on coat color or size, factors that may influence feeding behavior of the putative vectors of Onchocerca lupi¸ the filarial nematode parasite causing this disease. One hundred twenty-five client-owned dogs diagnosed with confirmed or suspected onchocerciasis. 1255 dogs without signs of this disease were utilized for comparison. Dogs lacking signs of canine onchocerciasis were assessed for coat color and weight. Proportions of dogs with these characteristics were used to predict signalment in a group of dogs with this disease, if the investigated characteristics were unrelated to disease risk. Predicted values were compared statistically with observed values in the diseased dog population. Black fly color preference was assessed utilizing black, brown, and white traps and statistically assessing any differences in trapping based on trap color. Results suggest that large, black dogs are more likely to develop canine ocular onchocerciasis than was predicted by chance alone (p=0.012). Results for smaller dogs with other coat colors were not significant. An increased risk for black dogs was supported by trapping data, as black flies were trapped significantly more often in black traps (p<0.001). While factors other than size and color probably also influence the likelihood of O. lupi infection and disease development, our results may assist clinicians in their diagnosis of canine ocular onchocerciasis and provide them with a tool to help educate their clients as to their dogs' risk of developing this disease.

A multicomponent screen for feeding behaviour and nutritional status in Drosophila to interrogate mammalian appetite-related genes

ObjectiveMore than 300 genetic variants have been robustly associated with measures of human adiposity. Highly penetrant mutations causing human obesity do so largely by disrupting satiety pathways in the brain and increasing food intake. Most of the common obesity-predisposing variants are in, or near, genes expressed highly in the brain, but little is known of their function. Exploring the biology of these genes at scale in mammalian systems is challenging. We sought to establish and validate the use of a multicomponent screen for feeding behaviour phenotypes, taking advantage of the tractable model organism Drosophila melanogaster. MethodsWe validated a screen for feeding behaviour in Drosophila by comparing results after disrupting the expression of centrally expressed genes that influence energy balance in flies to those of 10 control genes. We then used this screen to explore the effects of disrupted expression of genes either a) implicated in energy homeostasis through human genome-wide association studies (GWAS) or b) expressed and nutritionally responsive in specific populations of hypothalamic neurons with a known role in feeding/fasting. ResultsUsing data from the validation study to classify responses, we studied 53 Drosophila orthologues of genes implicated by human GWAS in body mass index and found that 15 significantly influenced feeding behaviour or energy homeostasis in the Drosophila screen. We then studied 50 Drosophila homologues of 47 murine genes reciprocally nutritionally regulated in POMC and agouti-related peptide neurons. Seven of these 50 genes were found by our screen to influence feeding behaviour in flies. ConclusionWe demonstrated the utility of Drosophila as a tractable model organism in a high-throughput genetic screen for food intake phenotypes. This simple, cost-efficient strategy is ideal for high-throughput interrogation of genes implicated in feeding behaviour and obesity in mammals and will facilitate the process of reaching a functional understanding of obesity pathogenesis.

The influence of density and dominance on Pacific white shrimp (Litopenaeus vannamei) feeding behaviour

Identifying correct stocking densities is an important concern for shrimp farming especially when intensification of shrimp production is growing in order to meet increasing demand. Increased stocking densities generate greater competition among individuals, reduced feeding efficiencies and increased water degradation. Little is known, however, about the effects of stocking density on feeding behaviour and conspecific interactions in commercial shrimp species. Therefore, the aim of the present study was to investigate the effects of density and dominance hierarchies on the feeding behaviour of Pacific white shrimp (Litopenaeus vannamei). Juvenile shrimp (7.91 ± 1.81 g, n = 56) were divided into three density treatments (low: 6.2 shrimp/m2, medium: 12.4 shrimp/m2 and high: 24.8 shrimp/m2) with four groups at each density. Groups of shrimp were placed with feed in test arenas and recorded for 20 min, with each group recorded eight times on consecutive days. Video analysis was conducted via automated observation using EthoVision tracking software. Dominance hierarchies were observed within each group and were found to remain stable across observations. Therefore the effect of dominance on feeding behaviour was investigated via comparisons of behaviour of the most dominant and subordinate shrimp in each group. Results showed a clear effect of both density and dominance on L. vannamei feeding. A greater relative feed consumption was observed in shrimp at the high density, even though less time was spent on the feeding area due to space limitations on the feeding tray. Lowest levels of feeding activity were found in the low-density treatments, coupled with higher levels of exploratory behaviour (i.e. moving, distance and velocity) indicative of a reduced feeding motivation. Smaller distances and extended contact times between individuals were found with increasing density. Dominance influenced feeding behaviour; subordinates spent longer on the feeding area whereas dominants explored the test arena more, however, these differences were minimised at high density. Findings from this study therefore revealed how L. vannamei at high density (24.8 shrimp/m2) maximized feeding behaviours while minimising feeding inefficiencies due to dominance interactions. The study also showcased tracking shrimp feeding behaviour automatically at a group level.

Effects of a moderate transition from 70% to 90% concentrate diet on early alterations in feeding behavior, rumen environment, reticulorumen motility, and blood acid–base status in beef heifers

The objective of this study was to determine the early responses to a moderate concentrate increase in cattle diets with regards to various behavioral and physiological responses in cattle. Eight ruminally cannulated Angus crossbred beef heifers were adapted to a 70% concentrate diet (T70) and then switched to a 90% concentrate diet (H90). Measurements of feeding behavior, ruminal pH and temperature, rumen motility, liquid passage rate, and blood acid–base status were conducted on T70 as well as first day (d1) and second day (d2) of high-grain H90feeding to monitor the beginning of the transition period. Ruminal pH was below 5.6 for longer on both days of H90 compared with T70, suggesting animals experienced subacute ruminal acidosis while switching from T70 to H90. Transitioning did not affect dry matter intake (DMI). Eating rate during meals was reduced on d1 and d2 H90 compared with T70. Ruminal contraction amplitude was reduced on both days of H90 feeding. Contraction duration was reduced on d1 H90, and returned to T70 values by d2 H90. Results indicated that a moderate transition to a finishing diet influenced feeding behavior and reduced rumen motility at the beginning time after transition, but did not influence voluntary DMI.

Latitudinal patterns in the diet of Andean condor (Vultur gryphus) in Chile: Contrasting environments influencing feeding behavior

Human-dominated environments alter the availability and quality of resources for many species, especially for scavengers that have large home ranges and plastic foraging behaviors that enable them to exploit novel resources. Along the western slope of the Andes, the modification of natural landscapes have resulted in significant declines in native prey, the introduction of non-native species, and an increase in the availability of anthropogenic resources. These factors have likely influenced the resources available to Andean condors (Vultur gryphus), however, data are lacking as to how condor's diet vary along their large latitudinal range. We evaluated differences in Andean condor diet along a ~2500 km latitudinal gradient in Chile from the heavily modified Central zone (32–34°S) to the more pristine Austral zone (44–56°S). We assessed diet composition through the identification of prey remains in condor pellets, and carbon and nitrogen isotope analysis of condor feathers and their primary prey identified from pellet analysis. Our results identified medium- and large-bodied domesticated mammals (ungulates) and introduced exotic species (lagomorphs) as common prey across the study area. Condors from the Central zone had the largest isotopic niche width, probably related to consumption of anthropogenic resources with distinctly high carbon isotope values indicative of C4-based foods likely acquired from landfills or corn-fed livestock. Isotopic niches for condors from the Southern and Austral zones almost completely overlapped. Andean condor diet is strongly influenced by local conditions determining differential access to prey sources. The high dependence of Andean condors on livestock across a large geographical area, and landfills in more (sub)urban areas, may help stabilize their populations via anthropogenic resources subsidies. Long-term dependence on such resources, however, may have health costs including contaminant exposure and greater mortality risk. These data will help identify potential threats related to resource availability and use, and better inform management and conservation decisions.

Time of Grain Supplementation and Social Dominance Modify Feeding Behavior of Heifers in Rotational Grazing Systems

Social hierarchy affects the access of animals to feed resources. On daily rotational pasture systems, supplementation time may influence feeding behavior. This trial was designed to test the effect of grain delivery time on the feeding behavior of heifers. Heifers divided into two groups according to breed (n = 15 Braford and n = 19 Jersey) were tested in a crossover design with two treatments: INITIAL—supplement at 8 am (entry time to a fresh paddock), and MIDDLE—supplement at 4 pm (middle time of paddock use). Animals entered a new paddock every morning, and grain supplement at 2 kg/animal/day was offered at the fence line (1 m/animal). Then, ingestive and other behaviors were registered by direct visual observation through scan sampling at 2-min intervals for 1 h after grain supply. Agonistic interactions were recorded continuously (instigator–victim) to build a social matrix whereby each heifer was defined as dominant, intermediate, or subordinate. Weekly pasture samples were collected according to the order that animals left the feeding area, using the hand-plucking technique, to determine crude protein and fiber content. Heifers spent more time grazing on the INITIAL treatment (p < 0.0001) but exhibited more behaviors on the MIDDLE treatment (p < 0.0001). Dominant heifers spent more time eating grain (p = 0.0008), whereas subordinate heifers spent more time grazing along the paddock (p = 0.0067), but not along the fence (p = 0.0008). The crude protein content of pasture samples was higher for the INITIAL treatment (p < 0.0001). Behavioral interaction occurred with respect to the order of leaving the feeding area, social rank, and crude protein consumed (p = 0.04). Subordinate heifers consistently grazed more and ate less grain supplement than dominant and intermediate heifers. However, when grain supplement was offered at the time animals entered the paddock, more grazing activity took place during supplement feeding, and subordinate heifers could select a high-protein diet. In the INITIAL treatment, this means that subordinate animals could benefit from the better pasture available, keeping a distance from dominant heifers, reducing agonistic interactions and likely improving their welfare.

Does Seasonality, Tidal Cycle, and Plumage Color Influence Feeding Behavior and Efficiency of Western Reef Heron (Egretta gularis)?

Simple SummaryWestern Reef Heron (Egretta gularis, Ardeidae) is a widely distributed species occurring in the Arabian Gulf in which individuals are either dark or light morphed. Most (over 70%) of the population consists of dark morphed birds and it is suggested that morphological characters are influenced by feeding behavior, predator–prey relations, and the environment. We studied the feeding behavior in the two color morphs to better understand the factors that influenced feeding behavioral diversity and efficiency. We recorded 13 feeding behavior types with difference in their utilization between seasons and age groups. Stand and wait and slowly walking were the two most commonly used techniques in both morphs. Feeding behavioral diversity was higher in both morphs in summer, probably because summers are harsh and abundance of food is lower. Feeding behavioral diversity was higher in dark morphs in general and was even higher in summer at falling tides. Foraging efficiency, however, did not vary between seasons or morphs. Feeding behavioral diversity and foraging efficiency was significantly higher during lag periods of rising tides in both morphs. Thus, it appears that dark morphs could be disadvantaged in summer months and therefore be utilizing a wider variety of behaviors to acquire adequate food. This study indicates that other factors, such as predator evasion or prey avoidance, may influence feeding behavior diversity and efficiency. Further studies are needed to help explain the high abundance of dark morphs in the region.Polymorphic traits may evolve in many species of birds, often driven by multiple environmental factors. It is hypothesized that polymorphic traits in herons could be influenced by feeding behavior. Most of the Western Reef Herons (Egretta egularis) (more than 70%) are of the dark morph in the United Arab Emirates (UAE). Feeding behavior and efficiency in the dimorphic Western Reef Heron was characterized in a shoreline habitat of Al-Zora Protected Area, Ajman, UAE in relation to season, tidal cycle, and color morphs. Foraging behavioral observations were made using standard focal birds during summer and winter seasons spanning entire tidal cycles. Western Reef Herons used 13 feeding behavior types with difference in their utilization between seasons and age groups. Stand and wait and slowly walking were the two most commonly used techniques in both morphs. Feeding behavioral diversity was higher in both morphs in summer, probably because summers are harsh and abundance of food is lower. Feeding behavioral diversity was higher in dark morphs in general and was even higher in summer during falling tides. Foraging efficiency, however, did not vary between seasons or morphs. Feeding behavioral diversity and foraging efficiency was significantly higher during lag periods of rising tides in both morphs. Thus, it appears that dark morphs could be disadvantaged in summer months and therefore be utilizing a wider variety of behaviors to acquire adequate food. This does not explain why there are more dark morphed birds (70%) in the population. We suggest that dark morphed birds compensate for lower feeding efficiency by increasing feeding behavioral diversity and feeding efficiency during the rising tides. Further studies are needed to evaluate the influence of prey avoidance and the choice of predators that attack herons, to better understand factors influencing the numerical dominance of dark morphs.

P.273 Borderline personality disorder: efficacy of clotiapine on aggression

Providing access to forage has been shown to influence feeding behavior and non-nutritive oral behavior in individually housed calves, and these effects may be enhanced or altered in calves reared in social housing. We evaluated the effect of hay provision on the behavioral development and performance of group-housed dairy calves. Holstein calves (n = 32) were group-housed (4 calves per group) at 17 ± 3 (mean ± SD) d of age. All calves were provided milk replacer (8 L/d) via an automated milk feeder and pelleted starter and water ad libitum. Pens were randomly assigned to receive either chopped coastal Bermuda grass in buckets adjacent to the starter trough (starter and hay, STH; n = 4 pens), or no additional feed (starter only, ST; n = 4 pens). Calves were weaned through a 10-d stepdown program beginning at 46 d of age. Intake of solid feed and hay were recorded daily, and body weights were measured weekly. The behavior of 2 focal calves per pen was recorded continuously from video for 12 h on 2 consecutive days during each of wk 4, 6, and 7 of life, to measure solid feed intake time, grooming, and pen-directed sucking. Hay provision influenced total feed intake, with calves provided hay having greater total solid feed intake in the week before weaning (0.79 vs. 0.55 kg/d, STH vs. ST, respectively; SE = 0.19). Average daily gain (ADG) was similar during the pre-weaning period but tended to be greater for STH calves during weaning. Calves in pens provided hay also had fewer unrewarded visits to the milk feeder during weaning (12.5 vs. 21.1 visits per 12 h, STH vs. ST, respectively; SE = 3.59) and performed less pen-directed sucking (9.11 vs. 19.3 min per 12 h, STH vs. ST, respectively; SE = 2.86). Self-grooming time and bout characteristics evolved differently between treatments over time, with pens of calves provided hay having a greater increase in frequency and duration of self-grooming bouts during weaning. Overall, we found that providing hay to pre-weaned calves resulted in behavioral and performance benefits, including greater total feed intake and reductions in pen-directed sucking, suggesting that access to hay may improve calf welfare.

Evaluating effects of providing hay on behavioral development and performance of group-housed dairy calves

Providing access to forage has been shown to influence feeding behavior and non-nutritive oral behavior in individually housed calves, and these effects may be enhanced or altered in calves reared in social housing. We evaluated the effect of hay provision on the behavioral development and performance of group-housed dairy calves. Holstein calves (n = 32) were group-housed (4 calves per group) at 17 ± 3 (mean ± SD) d of age. All calves were provided milk replacer (8 L/d) via an automated milk feeder and pelleted starter and water ad libitum. Pens were randomly assigned to receive either chopped coastal Bermuda grass in buckets adjacent to the starter trough (starter and hay, STH; n = 4 pens), or no additional feed (starter only, ST; n = 4 pens). Calves were weaned through a 10-d stepdown program beginning at 46 d of age. Intake of solid feed and hay were recorded daily, and body weights were measured weekly. The behavior of 2 focal calves per pen was recorded continuously from video for 12 h on 2 consecutive days during each of wk 4, 6, and 7 of life, to measure solid feed intake time, grooming, and pen-directed sucking. Hay provision influenced total feed intake, with calves provided hay having greater total solid feed intake in the week before weaning (0.79 vs. 0.55 kg/d, STH vs. ST, respectively; SE = 0.19). Average daily gain (ADG) was similar during the pre-weaning period but tended to be greater for STH calves during weaning. Calves in pens provided hay also had fewer unrewarded visits to the milk feeder during weaning (12.5 vs. 21.1 visits per 12 h, STH vs. ST, respectively; SE = 3.59) and performed less pen-directed sucking (9.11 vs. 19.3 min per 12 h, STH vs. ST, respectively; SE = 2.86). Self-grooming time and bout characteristics evolved differently between treatments over time, with pens of calves provided hay having a greater increase in frequency and duration of self-grooming bouts during weaning. Overall, we found that providing hay to pre-weaned calves resulted in behavioral and performance benefits, including greater total feed intake and reductions in pen-directed sucking, suggesting that access to hay may improve calf welfare.

Adiponectin Decreases Gastric Smooth Muscle Cell Excitability in Mice

Some adipokines known to regulate food intake at a central level can also affect gastrointestinal motor responses. These are recognized to be peripheral signals able to influence feeding behavior as well. In this view, it has been recently observed that adiponectin (ADPN), which seems to have a role in sending satiety signals at the central nervous system level, actually affects the mechanical responses in gastric strips from mice. However, at present, there are no data in the literature about the electrophysiological effects of ADPN on gastric smooth muscle. To this aim, we achieved experiments on smooth muscle cells (SMCs) of gastric fundus to find out a possible action on SMC excitability and on membrane phenomena leading to the mechanical response. Experiments were made inserting a microelectrode in a single cell of a muscle strip of the gastric fundus excised from adult female mice. We found that ADPN was able to hyperpolarize the resting membrane potential, to enhance the delayed rectifier K+ currents and to reduce the voltage-dependent Ca2+ currents. Our overall results suggest an inhibitory action of ADPN on gastric SMC excitation–contraction coupling. In conclusion, the depressant action of ADPN on the gastric SMC excitability, here reported for the first time, together with its well-known involvement in metabolism, might lead us to consider a possible contribution of ADPN also as a peripheral signal in the hunger–satiety cycle and thus in feeding behavior.

Hedgehog Pathway Activation Alters Ciliary Signaling in Primary Hypothalamic Cultures.

Primary cilia dysfunction has been associated with hyperphagia and obesity in both ciliopathy patients and mouse models of cilia perturbation. Neurons throughout the brain possess these solitary cellular appendages, including in the feeding centers of the hypothalamus. Several cell biology questions associated with primary neuronal cilia signaling are challenging to address in vivo. Here we utilize primary hypothalamic neuronal cultures to study ciliary signaling in relevant cell types. Importantly, these cultures contain neuronal populations critical for appetite and satiety such as pro-opiomelanocortin (POMC) and agouti related peptide (AgRP) expressing neurons and are thus useful for studying signaling involved in feeding behavior. Correspondingly, these cultured neurons also display electrophysiological activity and respond to both local and peripheral signals that act on the hypothalamus to influence feeding behaviors, such as leptin and melanin concentrating hormone (MCH). Interestingly, we found that cilia mediated hedgehog signaling, generally associated with developmental processes, can influence ciliary GPCR signaling (Mchr1) in terminally differentiated neurons. Specifically, pharmacological activation of the hedgehog-signaling pathway using the smoothened agonist, SAG, attenuated the ability of neurons to respond to ligands (MCH) of ciliary GPCRs. Understanding how the hedgehog pathway influences cilia GPCR signaling in terminally differentiated neurons could reveal the molecular mechanisms associated with clinical features of ciliopathies, such as hyperphagia-associated obesity.

Sensory perception drives food avoidance through excitatory basal forebrain circuits.

Appetite is driven by nutritional state, environmental cues, mood, and reward pathways. Environmental cues strongly influence feeding behavior, as they can dramatically induce or diminish the drive to consume food despite homeostatic state. Here, we have uncovered an excitatory neuronal population in the basal forebrain that is activated by food-odor related stimuli, and potently drives hypophagia. Notably, we found that the basal forebrain directly integrates environmental sensory cues to govern feeding behavior, and that basal forebrain signaling, mediated through projections to the lateral hypothalamus, promotes selective avoidance of food and food-related stimuli. Together, these findings reveal a novel role for the excitatory basal forebrain in regulating appetite suppression through food avoidance mechanisms, highlighting a key function for this structure as a potent integrator of sensory information towards governing consummatory behaviors.

Immunoglobulin G modulation of the melanocortin 4 receptor signaling in obesity and eating disorders

Melanocortin 4 receptor (MC4R) plays a key role in regulation of appetite activated by its main ligand α-melanocyte-stimulating hormone (α-MSH) in both central and peripheral targets. α-MSH also binds to circulating immunoglobulins (Igs) but the functional significance of such immune complexes (ICs) in MC4R signaling in normal and pathological conditions of altered appetite has remained unknown. To address this question, we analyzed plasma levels, affinity kinetics, and binding epitopes of α-MSH-reactive IgG extracted from plasma samples of female patients with hyperphagic obesity, anorexia nervosa, bulimia nervosa, binge-eating disorder, and healthy controls. Ability of α-MSH/IgG IC to bind and activate human MC4R were studied in vitro and to influence feeding behavior in vivo in rodents. We found that α-MSH-reactive IgG were low in obese but increased in anorectic and bulimic patients and displayed different epitope and kinetics of IC formation. Importantly, while α-MSH/IgG IC from all subjects were binding and activating MC4R, the receptor binding affinity was decreased in obesity. Additionally, α-MSH/IgG IC had lower MC4R-mediated cAMP activation threshold as compared with α-MSH alone in all but not obese subjects. Furthermore, the cellular internalization rate of α-MSH/IgG IC by MC4R-expressing cells was decreased in obese but increased in patients with anorexia nervosa. Moreover, IgG from obese patients prevented central anorexigenic effect of α-MSH. These findings reveal that MC4R is physiologically activated by IC formed by α-MSH/IgG and that different levels and molecular properties of α-MSH-reactive IgG underlie biological activity of such IC relevant to altered appetite in obesity and eating disorders.

Mechanism of Acetic Acid Gustatory Repulsion in Drosophila

The decision to consume or reject a food based onthe degree of acidity is critical for animal survival. However, the gustatory receptors that detect sourcompounds and influence feeding behavior have been elusive. Here, using the fly, Drosophila melanogaster, we reveal that a member of the ionotropic receptor family, IR7a, is essential for rejecting foods laced with high levels of acetic acid. IR7a is dispensable for repulsion of other acidic compounds, indicating that the gustatory sensation of acids occurs through a repertoire rather than asingle receptor. The fly's main taste organ, thelabellum, is decorated with bristles that house dendrites of gustatory receptor neurons (GRNs). IR7a is expressed in a subset ofbitter GRNs ratherthan GRNs dedicated to sour taste. Our findings indicate that flies taste acids through a repertoire of receptors, enabling them to discriminate foodson the basis of acid composition rather than just pH.

Chicken or the Egg: The Reciprocal Association Between Feeding Behavior and Animal Welfare and Their Impact on Productivity in Dairy Cows.

Feeding behavior in dairy cattle has a significant impact on feed efficiency, which is important for increasing the profitability of livestock and, at the same time, reducing the environmental impact. Feeding behavior can be measured by feeding time, meal duration, meal frequency, feeding rate, and rumination time. Higher feed intake is related to lower feed efficiency; whereas, an increase in feeding time facilitates chewing, reduces feed particle size and increases its digestibility. More frequent and shorter meals are usually associated with a more efficient use of feed due to improvement of feed digestibility. Rumination time is positively associated with milk production. Impaired health is associated with variations in feeding behavior, which can be used to identify and predict some diseases such as ketosis, mastitis, or lameness. Changes in rumination time are also a reliable indicator of mastitis, lameness, ketosis, abomasal displacement, and the onset of calving. In addition to the cause-effect relationship between disease and changes in feeding behavior, there are also some cases in which changes in feeding behavior may lead to an increased risk of disease, as exemplified by the relationship of feeding rate with sub-acute ruminal acidosis. Feeding behavior is regulated by internal and external factors and some of them are relevant for animal welfare. The main welfare-associated factors influencing feeding behavior are social behavior and temperament, and environmental effects. Cattle are social animals and hierarchy has a notable impact on feeding behavior, especially when access to feed is limited. Competition for feed causes a reduction in the average feeding time but increases feeding rate. Excitable animals visit the feeder more often and spend less time per meal. High environmental temperature affects feeding behavior, as heat-stressed cattle change their feeding pattern by concentrating the feeding events in crepuscular hours, leading to an increased risk of sub-acute ruminal acidosis. In conclusion, feeding behavior is a determinant feature for improving efficiency, productivity and welfare of dairy cattle. Routine assessment of feeding behavior allows monitoring of health and production status of dairy cattle at the individual and farm level, which is a useful tool to optimize the management of livestock.

Influences of maternal nutrient restriction and arginine supplementation on visceral metabolism and hypothalamic circuitry of offspring

Maternal nutrient restriction during gestation can exert long-term negative effects on offspring health and performance. Arginine supplementation may rescue some of the negative effects elicited by maternal nutrient restriction. We tested the hypothesis that maternal arginine supplementation during gestation would rescue deleterious effects of nutrient restriction on in vitro O2 consumption in the liver and jejunum and hypothalamic protein expression of proopiomelanocortin (POMC), neuropeptide Y (NPY), agouti-related peptide (AgRP), and neuronal nitric oxide synthase (nNOS), and the colocalization of nNOS and active phosphor-signal transducer and activator of transcription 3 (pSTAT3) in female offspring. Multiparous ewes were assigned to dietary treatment at 54 d of gestation: 100% of requirements (Con), 60% of control (Res), or Res plus rumen-protected arginine (Res-Arg; 180 mg/kg). At parturition, offspring were immediately removed from their dam and placed on a common diet. At 54 ± 4 d of age, female lambs (n = 6 per treatment) were weighed, the liver and jejunum were weighed, and samples were collected for in vitro measurement of O2 consumption. The hypothalamus was collected to determine protein expression of POMC, NPY, AgRP, and nNOS, and the colocalization of nNOS and pSTAT3 (n = 3, 4, and 4 for Con, Res, and Res-Arg, respectively). Hepatic consumption of O2 in vitro (mol/min/liver) was decreased (P = 0.04) in the Res and Res-Arg group compared with Con. Intensity of staining for NPY-containing fibers tended to decrease (P = 0.10) in Res and Res-Arg compared with Con. Number of POMC neuronal cells in the arcuate nucleus (ARC) of the hypothalamus decreased (P ≤ 0.03) in the Res group compared with Res-Arg. These observations demonstrate that maternal nutrient restriction decreases energy utilization in the liver and number of POMC cells in the ARC of offspring. Supplementation of arginine to the gestating ewe failed to influence hepatic use of energy in lambs from Res ewes. Numbers of POMC-containing cells were increased in the ARC in lambs from ewes restricted to 60% of nutritional requirements and supplemented with rumen-protected arginine, potentially influencing feeding behavior and hepatic energy metabolism.

Influence of feeding direct-fed microbial supplementation on growth performance and feeding behavior in naturally fed and conventionally fed finishing cattle with different dietary adaptation periods.

To determine the effects of finishing system (conventional vs. natural), dietary adaptation length (14 vs. 28 d), and direct-fed microbial (DFM) supplementation (no DFM vs. DFM) on growth performance and feeding behavior, 120 yearling steers (390 ± 2.8 kg) were used in a completely randomized design with a 2 × 2 × 2 factorial arrangement of treatments. Feed intake was monitored using the Insentec feeding system. Blood samples were collected every 28 d. After 140 d on feed, steers were slaughtered and carcass characteristics collected. Conventionally fed steers had greater (P ≤ 0.001) final BW, carcass weight, and dressing %. Dry matter intake was not influenced (P ≥ 0.31) by treatment. Length of dietary adaptation period did not influence (P ≥ 016) final BW, ADG, and G:F. There was a feeding system × DFM interaction (P ≤ 0.02) for ADG and G:F with conventionally fed steers fed DFM having the greatest (P ≤ 0.05) and naturally fed steers supplemented with DFM having the least (P ≤ 0.05) ADG and G:F. Number of visits to the feed bunk and number of meals per day did not differ (P > 0.05) among treatments. Time eating per visit and per meal was greater (P = 0.05) in steers supplemented with DFM than in steers not supplemented with DFM. On day 56 and 140, plasma glucose concentration was greater (P ≤ 0.03) in steers adapted in 14 d than in steers adapted in 28 d. On day 84, plasma glucose concentration was greater (P = 0.02) in naturally fed compared to conventionally fed steers. On day 112, there was a dietary adaptation period × DFM interaction (P = 0.004) for plasma glucose concentration with glucose concentration greatest (P ≤ 0.05) in steers adapted in 14 d supplemented with DFM and in steers adapted in 28 d not supplemented with DFM, least for steers adapted in 28 d supplemented with DFM, with steers adapted in 14 d not supplemented with DFM intermediate (P ≤ 0.05). On day 112 and 140, plasma urea N concentration was greater (P ≤ 0.05) in steers adapted in 28 d than in steers adapted in 14 d. These data indicate that conventionally fed steers generally had improved growth performance compared to naturally fed steers. Length of dietary adaptation and DFM supplementation had minimal effects on growth performance but did interact with feeding system to influence feeding behavior and blood metabolite concentrations.

Estradiol and the control of feeding behavior

This review lays out the evidence for the role of E2 in homeostatic and hedonic feeding across several species. While significant effort has been expended on homeostatic feeding research, more studies for hedonic feeding need to be conducted (i.e. are there increases in meal size and enhanced motivation to natural food rewards). By identifying the underlying neural circuitry involved, one can better delineate the mechanisms by which E2 influences feeding behavior. By utilizing more selective neural targeting techniques, such as optogenetics, significant progress can be made toward this goal. Together, behavioral and physiological techniques will help us to better understand neural deficits that can increase the risk for obesity in the absence of E2 (menopause) and aid in developing therapeutic strategies.

Fatty Acids, Antioxidants and Physical Activity in Brain Aging.

Polyunsaturated fatty acids and antioxidants are important mediators in the central nervous system. Lipid derivatives may control the production of proinflammatory agents and regulate NF-κB activity, microglial activation, and fatty acid oxidation; on the other hand, antioxidants, such as glutathione and ascorbate, have been shown to signal through transmitter receptors and protect against acute and chronic oxidative stress, modulating the activity of different signaling pathways. Several authors have investigated the role of these nutrients in the brains of the young and the aged in degenerative diseases such as Alzheimer’s and Parkinson’s, and during brain aging due to adiposity- and physical inactivity-mediated metabolic disturbances, chronic inflammation, and oxidative stress. Through a literature review, we aimed to highlight recent data on the role of adiposity, fatty acids, antioxidants, and physical inactivity in the pathophysiology of the brain and in the molecular mechanisms of senescence. Data indicate the complexity and necessity of endogenous/dietary antioxidants for the maintenance of redox status and the control of neuroglial signaling under stress. Recent studies also indicate that omega-3 and -6 fatty acids act in a competitive manner to generate mediators for energy metabolism, influencing feeding behavior, neural plasticity, and memory during aging. Finding pharmacological or dietary resources that mitigate or prevent neurodegenerative affections continues to be a great challenge and requires additional effort from researchers, clinicians, and nutritionists in the field.