Influence Of Spermine Research Articles

The Influence of Spermine, Spermidine and Various Sera on T-Lymphocyte and Granulocyte Colony Growth in vitro

Using T-lymphocyte (T-LC) and granulocyte colony (GC) assays with truly proliferating cells, the dose-response relationships of spermine and spermidine, fetal calf (FCS), calf (CS), horse (HS) and human AB serum (ABS), and of the polyamines in the presence of selected does of the sera were analyzed. In contrast to earlier observations using [3H]thymidine uptake masurements it was found that the polyamines as well as the sera themselves inhibit T-LC and GC growth in the presence of autologous HS and ABS, respectively. The polyamines also inhibit in the presence of additional HS and ABS, yet most effectively with added FCS and CS. Thus the previously reported species-specificity of the serum-dependent polyamine inhibition has here been shown to be more quantitative than qualitative. These studies stress the significance of assays utilizing truly dividing cells and broad dose-response relationship in order to assess specific biological effects in vitro.

Influence of spermine on amino acid incorporation by free, bound, and reattached ribosomes from rat liver.

Influence of spermine on amino acid incorporation by free, bound, and reattached ribosomes from rat liver.

Influence of spermine and reduced temperature on the ability of transplanted nuclei to promote normal development in eggs of Rana pipiens.

Previous work has shown that nuclei of young embryos (to gastrula stage) promote normal development when transplanted into enucleated eggs. As development proceeds to later stages, the nuclei become increasingly restricted in their capacity to promote normal cleavage and development, and the majority of the embryos that do develop possess abnormal chromosome complements (see references 1 through 4). Experiments reported in this paper show that a reduction in the temperature at which the transplantation operation is performed (from 20-11 degrees C) improves the capacity of the nuclei to promote cleavage of recipient eggs, but appears not to improve later development. The capacity to promote postgastrula development is significantly improved when the donor cells are exposed to the polyamine, spermine, prior to and during nuclear transfer. This improvement is correlated with a reduction in the incidence of chromosomal abnormalities. The mechanism of this effect of spermine, and its relationship to the nuclear differentiation problem, is considered.

Influence of spermine and histones on a nuclear nucleosidase with ADP-ribose transferase activity in ehrlich ascites cells

Influence of spermine and histones on a nuclear nucleosidase with ADP-ribose transferase activity in ehrlich ascites cells

Influence of spermine and related substances on susceptibility of Bacillus anthracis to lysozyme.

SummarySpermine was active at a concentration of 3 μ moles per liter in allowing growth of a lysozyme-susceptible strain of B. anthracis in the presence of 1-4000 lysozyme. Spermidine. 1-3 propanediamine, putrescine, and cadaverine were respectively 1/2, 1/20, 1/40 and 1/200 as active as spermine. The activity of all of the substances was overcome by addition of 0.005M sodium bicarbonate to the medium.