Influence Of Silver Nanoparticles Research Articles

Influence of Silver Nanoparticles on Onion (Allium cepa L.) Seed Germination and Seedling Vigor

During this study on the influence of silver nanoparticles (AgNPs) on growth of L-883 onion varieties Allium cepa L. AgNPs 100 nm size were used in four different concentrations (25, 50, 75, and 100 ppm) and T0 was kept as control. The seeds of onion were brought from NHRDF Indore. The findings revealed that 25 ppm of silver nanoparticle led to the highest seed germination percentage as compared to other concentrations. On the other hand, all the other concentrations were found to have a harmful effect on both seed germination and seedling growth as the level of toxicity are strongly associated with concentration of the suspensions. AgNPs of 100ppm concentration was found to have lowest rate of seed germination, however non-significant difference was found in 75ppm and 100ppm concentrations. Therefore, a concentration of 25ppm of AgNPs proved to be the most effective treatment for promoting optimal seed germination and seedling growth in onions.

A capacitive humidity sensor based on nanodiamond/silver nanoparticles (ND/Ag) nanocomposite with high stability and rapid response for respiratory monitoring

In this work, a high-performance capacitive humidity sensor based on nanodiamond/silver nanoparticles (ND/Ag) nanocomposite is proposed. The influence of silver nanoparticles with different content in nanodiamond is investigated, and the optimum ratio of 5 wt% Ag in the composite shows the maximum humidity sensing performance, which is three times than that of the pure nanodiamond based humidity sensor. Moreover, the proposed sensor exhibits a superior repeatability, ultrafast response time (<2 s) and recovery time (<1 s). More importantly, the sensor can maintain a good repeatability and rapid response speed when exposure to high humidity for a long time, demonstrating the excellent stability of the proposed sensor. Furthermore, complex impedance spectroscopy was applied to account for the underlying sensing mechanisms of the ND/Ag composite film humidity sensor. Finally, we design a circuit to realize non-contact breathing control by using the fabricated humidity sensor, which demonstrate that the fabricated humidity sensor could be applied to some auto-control systems by human-breath. This work demonstrated that ND/Ag composite film is an ideal candidate material for constructing high-performance humidity sensors for respiratory applications.

Influence of Silver Nanoparticles on the Metabolites of Two Transgenic Soybean Varieties: An NMR-Based Metabolomics Approach.

This study investigated the effect of AgNPs and AgNO3, at concentrations equivalent, on the production of primary and secondary metabolites on transgenic soybean plants through an NMR-based metabolomics. The plants were cultivated in a germination chamber following three different treatments: T0 (addition of water), T1 (addition of AgNPs), and T2 (addition of AgNO3). Physiological characteristics, anatomical analyses through microscopic structures, and metabolic profile studies were carried out to establish the effect of abiotic stress on these parameters in soybean plants. Analysis of the 1H NMR spectra revealed the presence of amino acids, organic acids, sugars, and polyphenols. The metabolic profiles of plants with AgNP and AgNO3 were qualitatively similar to the metabolic profile of the control group, suggesting that the application of silver does not affect secondary metabolites. From the PCA, it was possible to differentiate the three treatments applied, mainly based on the content of fatty acids, pinitol, choline, and betaine.

Nanoparticles Partially Restore Bacterial Susceptibility to Antibiotics.

The growing resistance of bacteria to antibiotics is one of the main public health problems nowadays. The influence of silver nanoparticle (AgNP) pretreatment of 220 cows with mastitis on the susceptibility of Staphylococcus epidermidis bacteria to 31 antibiotics was studied. The obtained results were compared with the previous results for Escherichia coli, Streptococcus dysgalactiae, and Staphylococcus aureus. For all four bacteria, an increase in susceptibility (9.5-21.2%) to 31 antibiotics after cow treatment with AgNPs was revealed, while after first-line antibiotic drug treatment as expected, the susceptibility decreased (11.3-27.3%). These effects were explained by (1) the increase in the contribution of isolates with efflux effect after antibiotic treatments and its decrease after AgNP treatment and (2) the changes in bacteria adhesion and anti-lysozyme activity after these treatments. The effect of the increasing antibacterial activity of antibiotics after AgNP treatment was the most pronounced in the case of E. coli and was minimal in the case of S. epidermidis. With AgNP treatment, the time of recovery decreased by 26.8-48.4% compared to the time of recovery after treatment with the first-line antibiotic drugs. The AgNP treatment allows for achieving the partial restoration of the activity of antibiotics.

Influence of Silver Nanoparticles on Germinated and Microwaved Chia Seeds (Salvia Hispanica L)

This study thoroughly investigated the impact of 60 ppm silver nanoparticles (AgNPs) on the germination process of chia seeds (Salvia Hispanica L.) and an examination of subsequent modifications induced by microwave treatment on AgNP-exposed seeds. The research findings highlighted a significant enhancement in seed germination percentage peaking at 81.67% on the fifth day particularly when exposed to a concentration of 60 ppm AgNPs. Concurrently, radicle length experienced a substantial increase indicative of improved early seedling growth. Morphological study was also studied by scanning electron microscopy. Results indicated that samples treated with microwave showed shrinkage and the samples treated with germination samples showed expansion. Furthermore, investigation of bioactive compounds indicated increased concentrations of total phenolic content and total flavonoids content in samples germinated with AgNPs simultaneously with increased antioxidant activity. In a nutshell, this study provided a comprehensive understanding of the impacts of 60 ppm AgNPs on germination and microwave treatment on chia seeds. The far-reaching implications span across agriculture, food science, and nutritional domains, providing a strong basis for potential future applications and further scientific examination.

Influence of Silver Nanoparticles on Microbiological and Freshness Quality Attributes of Beef Sausage and Fish Patties During Cryopreservation

Influence of Silver Nanoparticles on Microbiological and Freshness Quality Attributes of Beef Sausage and Fish Patties During Cryopreservation

The influence of silver nanoparticles on the process of epithelial transition in the context of cancer metastases.

Exposure to nanoparticles (NPs) can occur in a variety of occupational situations. Ultrafine particles of natural and anthropological origin toxicity has been described in epidemiological studies. Meanwhile, the risks associated with NPs exposure are not comprehensively assessed. A wide spectrum of NPs toxicity has been demonstrated, mainly through the induction of oxidative stress and inflammatory mediators. Among the newly described mechanisms of NPs toxicity is the induction of fibrosis via the epithelial-mesenchymal transition (EMT), which is also a key mechanism of cancer metastasis. The effect of NPs on EMT in the context of metastasis has not been sufficiently described so far, and the results of studies do not allow for the formulation of unambiguous conclusions. Therefore, the aim of the work was to determine the biological activity of silver NPs against MDA-MB-436 triple-negative breast cancer cells. Exposure to nanoparticles (NPs) can occur in a variety of occupational situations. Ultrafine particles of natural and anthropological origin toxicity has been described in epidemiological studies. Meanwhile, the risks associated with NPs exposure are not comprehensively assessed. A wide spectrum of NPs toxicity has been demonstrated, mainly through the induction of oxidative stress and inflammatory mediators. Among the newly described mechanisms of NPs toxicity is the induction of fibrosis via the epithelial-mesenchymal transition (EMT), which is also a key mechanism of cancer metastasis. The effect of NPs on EMT in the context of metastasis has not been sufficiently described so far, and the results of studies do not allow for the formulation of unambiguous conclusions. Therefore, the aim of the work was to determine the biological activity of silver NPs against MDA-MB-436 triple-negative breast cancer cells. Silver nanoparticles (AgNPs) cause a statistically significant increase in relative expression of all tested mesenchymal EMT markers - cadherin 2, vimentin, matrix metalloproteinase 2 and matrix metalloproteinase 9. At the same time, reduction of epithelial cadherin 1 expression was observed. The level of MDA-MB-436 migration and TGF-beta 1 secretion was slighty increased in AgNPs-treated cells, with no influence on invasion potential. Potentially prometastatic effect of AgNPs encourages further work on the safety of nanomaterials. Med Pr Work Health Saf. 2023;74(6):541-8.

Influence of Silver Nanoparticles on Color Stability of Room-Temperature-Vulcanizing Maxillofacial Silicone Subjected to Accelerated Artificial Aging

In this in vitro study, we assessed the color stability of an A-2186 room-temperature-vulcanizing (RTV) silicone elastomer by incorporating silver nanoparticles during accelerated artificial aging. Using five intrinsic silicone pigment types, including no pigment (colorless), red, blue, mocha, and a combination of the three, we created 160 disk-shaped specimens. These were evenly distributed across 20 experimental groups, each containing 8 samples (n = 8). The specimens underwent aging for 250 and 500 h in an artificial aging chamber. A colorimeter was used to measure the values of L*a*b* according to the Commission Internationale de L’Éclairage (CIE) standards. The 50:50% perceptibility threshold (∆E* = 1.1) and acceptability threshold (∆E* = 3.0) were used in the interpretation of the recorded color differences. At the 0.05 level of significance, the one-way analysis of variance (ANOVA) and Tukey post hoc test were used in the statistical analysis. Fourier-transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) showed that 0.2% AgNPs after 500 h of aging protected the silicone elastomer matrix and all characteristic bonds of the silicone elastomer. In contrast, silicone without AgNPs showed the distortion of all bonds after 500 h. Chromatic alterations (∆E* &gt; 0) were observed in all specimen groups, surpassing the perceptible threshold (1.1 units), except for mocha, with 0.2% AgNPs after 250 h of aging, which remained below the perceptible threshold (∆E* = 0.97). All groups demonstrated ∆E* values below the acceptable threshold, except for the red color, which exhibited a highly significant color change (p = 0.000). This study determined that all specimens, including colorless silicone, underwent color changes (∆E* &gt; 0), with red displaying a notably significant chromatic alteration. Additionally, AgNPs demonstrated substantial protection of the silicone and reduced the color change across all groups and colors, with enhanced efficacy corresponding to higher AgNP concentrations (0.2% AgNPs &gt; 0.15% AgNPs &gt; 0.1% AgNPs).

Influence of silver nanoparticles on mRNA expression of thyroid hormone-related genes in the thyroid gland and liver of laying hens

The widespread use of silver nanoparticles (AgNPs) in consumer products and animal husbandry raises the need to study their impact on living organisms. This study was conducted on Hy-Line Brown hens at the age of 25 weeks with an average weight of 1.58 kg. Hens for 2 weeks received a solution of 50 nm AgNPs at a concentration of 100 pm (experimental group; n = 6) or a solution in which the nanoparticles were suspended (control group; n = 6). Thyroid hormones (thyroxine – T4, triiodothyronine – T3) were evaluated in the blood plasma and expression profiles of genes involved in thyroid hormone (TH) synthesis (TSHR, NIS, TPO, TG), metabolism (DIO1, DIO2, DIO3) and transport (MCT8, MCT10, LAT1) were determined in the chicken thyroid gland. Furthermore, iodothyronine deiodinase, TH transporter and TH receptor (THRA, THRB) mRNA expressions were evaluated in the livers isolated from the same chickens. AgNPs did not affect serum T4 levels but elevated serum T3 concentration. The results showed that AgNPs increased DIO3 mRNA in the thyroid gland. In turn, in the liver AgNPs administration significantly upregulated DIO2 and downregulated MCT10 mRNA levels. These results indicate that exposure to AgNPs leads to a tissue-specific alternative expression of genes engaged in TH metabolism. Moreover, the mRNA expression of DIO2 in the liver showed a positive correlation with plasma T3 levels. In conclusion, AgNPs may have an impact on TH metabolism by affecting deiodinases and TH transporter MCT10 mRNA expression.

Synthesis, Characterization and Influence of Silver Nanoparticles Against Phases of Musca Domestica

The achievement of this research was to study the influence of AgNPs on the life stages of Musca domestica. The supernatant of Trichoderma harzianum successfully reduces AgNO3 into AgNPs through the duration of incubation, as noticed by the color change from white to red. The absorbance of UV–visible light results in an absorbance peak at 418 nm for nanoparticle solutions of Ag particles. The scanning electron microscopy (SEM) image confirmed the formation of AgNPs with an average particle size of 46.63 nm without aggregation or adhesion. The microscopy of energy-dispersive X-ray (EDX) approved the formation of AgNPs with a high amount of some atoms of carbon and oxygen derivatives from components of fungus extract used to reduce AgNO3. The concentration of AgNPs prepared with serial dilutions of 5%, 10%, 15%, 20% and 25%, also with sterilized distilled water as a control and the influence of AgNPs on phases of [Formula: see text] domestica had been studied. The eggs of the studied insects were affected by 20% of AgNPs, which showed an increase in incubation time of 11 h without any hatched eggs at 25%. The larval stage of the insect was also influenced by AgNPs solution, which at 25% increased the duration of incubation to reach 11 h with 27 larval insects dead, compared with 7 h without any missing membrane in the larval stage of the insect. As well as the pupal phase of insects, we increased the period of incubation to 10 h with 7 h in control, and in addition, the number of pupal insects dead reached 23 in 25% concentration. Finally, the AgNPs also increased the number of adult insects to reach 27 at 25% concentration. The AgNPs approved the activity toward [Formula: see text] domestica and therefore considered it a preferable option for biological control.

Influence of silver nanoparticles on postharvest disease, pericarp hardening, and quality of mangosteen

The main problem associated with mangosteen (Garcinia mangostana L.) in the postharvest period is fruit rot caused by Lasiodioplodia theobromae, which results in pericarp hardening, poor quality, and short storage life. Silver nanoparticles (AgNPs) constitute a potential agent for curbing microbial infections, especially fungal infections. In the in vitro test conducted in this study, 270 ppm AgNPs completely inhibited mycelium growth and spore germination (100 %) in L. theobromae, similar to the results obtained using 200 ppm fungicidal carbendazim. For the in vivo test, the concentration of AgNPs was increased to 300 ppm to provide a strong inhibitory effect against fungal infection on mangosteen fruit. The results showed that treatment with AgNPs significantly decreased the disease index (DI) in the artificially inoculated mangosteen fruit stored at 25ºC, and AgNP residue in the fruit was found to be 3.3 mg kg-1, which conforms to the Environmental Protection Agency (2009) regulations. Furthermore, the effects of AgNPs on fruit rot disease and plant defense-related enzymes were investigated with regard to naturally infected mangosteen stored at 13ºC for 30 days, as compared to fungicide-treated fruits and non-treated fruits (control). The results revealed that AgNP treatment resulted in an eight-fold reduction in fruit rot disease, whereas fungicidal treatment resulted in only a 2.46-fold disease reduction compared to the result in the control group. Also, AgNP treatment induced plant defense-related enzyme activities: phenylalanine ammonia-lyase (PAL), peroxidase (POD), chitinase (CHI) and β-1,3-glucanase (GLU), and ɑ-mangostin content (a major phenolic compound in pericarp). Further, the effect of AgNPs on the physiology and quality of fruits was measured and compared with the non-treated fruits. The results showed that AgNP treatment lowered ethylene production and rate of respiration, which resulted in a reduction in weight loss and pericarp hardening. AgNP treatment was also found to maintain pericarp color, chlorophyll content in calyx as well as total soluble solids (TSS), titratable acidity (TA), and anthocyanin content in the pericarp. This result implies that AgNPs constitute an effective and safe treatment modality for controlling fruit rot disease, reducing pericarp hardening, and maintaining the quality of mangosteen fruit.

Influence of Silver Nanoparticles on Photosynthetic Pigment Content and Mineral Uptake in Pineapple Seedlings Grown In Vitro under Aluminum Stress

The presence of toxic metals such as aluminum is described as a factor that could lead to a significant decrease in crop productivity, particularly for the cultivation of acidophilic plants. In the present study, in vitro cultivation of pineapple was used as a model to evaluate the role of silver nanoparticles (0.005, 0.01, 0.025, 0.05, and 0.1 g L−1 Ag NPs) against the negative effects of aluminum (0, 100, 300, and 500 μM AlCl3). The results showed that the presence of 0.025 g L−1 Ag NPs stimulated a higher concentration of photosynthetic pigments “a”, “b”, “a + b” and carotenoids in treatments with high levels of AlCl3. The application of Ag NPs allowed better shoot formation, improved chlorophyll a/b, and total/carotenoid ratios, as well as better levels of proline biosynthesis in response to stress. The synergistic interaction of Ag NPs and AlCl3 increased the availability and assimilation of mineral elements (K, Ca, Mg, Fe, Cu, Mn, and Zn) while decreasing Al translocation. In conclusion, the implementation and validation of Ag NPs in agricultural fields would be revolutionary because they represent a novel alternative for overcoming the limitations imposed by the presence of Al.

Influence of Silver Nanoparticles on the Growth of Ascitic and Solid Ehrlich Adenocarcinoma: Focus on Copper Metabolism.

The link between copper metabolism and tumor progression motivated us to use copper chelators for suppression of tumor growth. We assume that silver nanoparticles (AgNPs) can be used for lowering bioavailable copper. Our assumption is based on the ability of Ag(I) ions released by AgNPs in biological media and interfere with Cu(I) transport. Intervention of Ag(I) into copper metabolism leads to the replacement of copper by silver in ceruloplasmin and the decrease in bioavailable copper in the bloodstream. To check this assumption, mice with ascitic or solid Ehrlich adenocarcinoma (EAC) were treated with AgNPs using different protocols. Copper status indexes (copper concentration, ceruloplasmin protein level, and oxidase activity) were monitored to assess copper metabolism. The expression of copper-related genes was determined by real-time PCR in the liver and tumors, and copper and silver levels were measured by FAAS. Intraperitoneal AgNPs treatment beginning on the day of tumor inoculation enhanced mice survival, reduced the proliferation of ascitic EAC cells, and suppressed the activity of HIF1α, TNF-α and VEGFa genes. Topical treatment by the AgNPs, which was started together with the implantation of EAC cells in the thigh, also enhanced mice survival, decreased tumor growth, and repressed genes responsible for neovascularization. The advantages of silver-induced copper deficiency over copper chelators are discussed.

Optoplasmonic biosensor for lung cancer telediagnosis: Design and simulation analysis

Applying quantum technology to dispatch face-to-face medical activities has generated significant interest. Unfortunately, the work on remote medical treatment soliciting quantum medication and information processing techniques is hard to observe. In this research, we proposed the Mach–Zehnder interferometer (MZI) based optoplasmonic biosensors (OPBs) with two homodyne detectors for remote-based lung cancer detection using classical and quantum mechanical principles. From the classical basis (Drude-Lorentz model and Kretschmann configuration), the influence of silver nanoparticles (Ag NPs) layers and biomolecule concentration on the performance of biosensors has been investigated. The different types of cancer cells for CL1-5, A549, and HT-29 have been used to analyze the sensitivity, and 319, 332, and 365 (deg/RIU) have been achieved, respectively. In addition, from quantum mechanical principles, the biosignals were conveyed through quantum teleportation in the form of the quantum state of light via fiber optics cable to enable precise remote detection of lung cancer. The obtained sensitivity and teleportation fidelity clearly reveal, the best candidacy of the proposed optoplasmonic biosensor for lung cancer telediagnosis.

Influence of silver nanoparticles on the structure and mechanical properties of porous titanium nickelide alloys

The aim of the work is to study the effect of silver doping on the structure and mechanical properties of porous titanium nickelide alloys obtained by self-propagating high-temperature synthesis (SHS). The structure of the samples was studied using X-ray diffraction analysis, scanning and transmission electron microscopy. The mechanical properties of porous alloys were studied in compression tests. An increase in the volume fraction of the B19&rsquo; martensitic phase, intermetallic compounds based on the Ti2Ni and a decrease in the volume fraction of austenite with the B2 structure with an increase in the silver content were established. An increase in the plasticity of porous titanium nickelide alloys with an increase in the concentration of silver in the composition while maintaining the ultimate strength was revealed. The research was carried out with financial support of the&nbsp;Russian Science Foundation under the Grant No. 22-72-10037, https://rscf.ru/project/22-72-10037/.

Evaluation of the effect of silver nanoparticles on the tribological and thermophysical properties of bio-lubricants

In this article, the effect of infusion of silver nanoparticles into the base oil lubricants was investigated. In this study, Jatropha and Pongamia oils were used as base oils and silver nanoparticles infused at 0.25%, 0.5%, and 0.75% weight-percentage of the base oils to create nano-based bio-lubricants. The influence of silver nanoparticles on the Tribological and Thermophysical properties of the base oils was analyzed through various test procedures. The Pin-on-disc tribometer test was done to measure friction and wear at various loads. Pin made of Aluminum metal matrix composite fabricated by stir casting method was used and the results revealed that the inclusion of silver nanoparticles significantly improved the friction and wear properties of the base oils. Pin-worn surface morphology was analyzed using a field emission scanning electron microscope and X-ray diffraction. Thermal conductivity and kinematic viscosity of nano-based bio-lubricants were evaluated as per ASTM standards and the results showed that both the thermal conductivity and kinematic viscosity increased with the infusion of silver nanoparticles.

Influence of Silver Nanoparticles on Selected Properties of Dental Porcelain: An in vitro study

This study was conducted to reveal antibacterial effect of silver nanoparticles incorporation on dental porcelain and its effect on flexural strength and surface hardness of porcelain.

Influence of silver nanoparticles on mechanical, thermal and antibacterial properties of poly (vinyl alcohol)/rice hull powder hybrid biocomposite films

AbstractEarth produces 2.12 billion tons of total wastes annually and nearly 2 million tons of petroleum‐based synthetic polymers each day. The majority of synthetic polymers were obtained by packaging applications. Biodegradable films(biofilms) can be a good alternative for pollution‐creating synthetic polymers. Rice hull powder (RHP) as reinforcing fillers, polyvinyl alcohol (PVA) as matrix along with silver nanoparticles (AgNPs) were used to prepare the biofilms. By using the solution casting method, five samples of PVA/RHP (1–5 mM) AgNPs biofilms were fabricated and characterized using Fourier transform infrared (FT‐IR), X‐Ray Diffraction Analysis (XRD), field emission scanning electron microscope (FESEM), Thermogravimetric Analysis (TGA) and differential scanning calorimetry beyond testing for their mechanical and antibacterial activities. The small spherical shape elements in the FESEM images clarify the presence of AgNPs. The intensities of observed peaks in the FT‐IR spectrum keep decreasing with the inclusion of AgNPs. The X‐ray diffractogram confirms the strong peaks at 2θ = 38.2°, 49.1°, 60.8°, &amp; 78.3° due to the presence of the AgNPs. The tensile strength and tensile modulus reached a maximum value of 35.5 and 871 MPa respectively. The presence of heat‐stable metallic silver made the biofilms thermally stable up to 371°C and also improved the antibacterial activity exhibited by a better inhibition zone. The improved results clarified that biofilms can be suggested for packaging applications.

Mechanism of Phanerochaete chrysosporium-mediated tetracycline hydrochloride removal promoted by low-dose silver nanoparticles

The influence of silver nanoparticles (AgNPs) on environmental microorganisms has been extensively reported, however, there is currently limited understanding of effects of stress-induced by AgNPs on other pollutant degradation. In this study, Phanerochaete chrysosporium was stimulated by different concentrations of AgNPs to explore its effect on P. chrysosporium-mediated degradation of tetracycline hydrochloride (TCH). The results indicate that treatment with AgNPs at low concentration resulted in a considerable increase in TCH degradation, TCH degradation reached a maximum of 97% with 0.1 mg L−1 AgNPs treated after 3 d, and the viability of P. chrysosporium increased 1.48 times compared with the control group. The largest O2 influx of − 17 pmol cm−2 s−1 under 0.1 mg L−1 AgNPs treatment reveal the enhancement of P. chrysosporium respiration. In addition, low concentrations of AgNPs could induce the overexpression of cytochrome P450 and extracellular degradation enzymes MnP-related genes in stimulated cells, which further induce the production of extracellular degrading enzymes (51.0 U L−1 MnP and 23.7 U L−1 LiP) to accelerate the degradation of TCH. Together, our combined results uncovered the enhanced degradation mechanisms of P. chrysosporium induced by which AgNPs under sublethal dosage.

Minimizing the Silver Free Ion Content in Starch Coated Silver Nanoparticle Suspensions with Exchange Cationic Resins.

This work describes the optimization of a methodology for the reduction of silver ions from silver nanoparticle suspensions obtained from low-yield laboratory procedures. The laboratory synthesis of silver nanoparticles following a bottom-up approach starting from silver nitrate, originates silver ions that were not reduced to their fundamental state for nanoparticles creation at the end of the process. However, it is well known that silver ions can easily influence chemical assays due to their chemical reactivity properties and can limit biological assays since they interfere with several biological processes, namely intracellular ones, leading to the death of living cells or organisms. As such, the presence of silver ions is highly undesirable when conducting biological assays to evaluate the influence of silver nanoparticles. We report the development of an easy, low-cost, and rapid methodology that is based on cation exchange resins to minimize the silver ion content in a raw suspension of silver nanoparticles while preserving the integrity of the nanomaterials. This procedure preserves the physical-chemical properties of the nanoparticles, thus allowing the purified nanoparticulate systems to be biologically tested. Different types of cationic resins were tested, and the developed methodology was optimized by changing several parameters. A reduction from 92% to 10% of free silver/total silver ratio was achieved when using the Bio-Rad 50W-X8 100–200 mesh resin and a contact time of 15 min. Filtration by vacuum was used to separate the used resin from the nanoparticles suspension, allowing it to be further reused, as well as the purified AgNPs suspension.