Influence Of Low-intensity Laser Radiation Research Articles

The influence of low-intensity laser radiation on the blood parameters of cows under technological stress

The study of clinical-hematologic parameters under technological stress at cows and the effect of low-intensity laser radiation (LILR) under technological stress was carried out. The study was carried out on high-yielding Holstein black-breed cows which were modeled technological stress and subsequently exposed to LILR on the withers or ear with exposure time of 5 or 15 depending on the group of animals. LILR with a wavelength of 830 nm was used. The study of hematological parameters of blood under technological stress in animals showed an increase in the number of leukocytes up to 14 days, a decrease in the number of erythrocytes and hemoglobin by 3 days of the experiment relative to the values of intact animals. At action of LILR under technological stress the increase of erythrocytes and hemoglobin in blood was registered. The content of leukocytes in blood corresponded to the level of intact animals, their functional activity increased. The concentration of reduced glutathione in animals under technological stress was decreased throughout the entire observation period. When using LILR under technological stress in animals, changes in reduced glutathione were less pronounced compared to animals after technological stress. When cows were exposed to LILR on the withers, an increase in the content of reduced glutathione in blood was registered. The obtained results indicate that the changes in blood parameters are directed to the activation of compensatory-adaptive reactions of the organism under the action of LILR under technological stress.

Effect of low intensity laser radiation on sugar content in sugar beet root crops obtained from seeds treated with a quantum generator

In the modern world the question of obtaining environmentally friendly food products with the best possible performance is increasingly raised not only in the scientific community but also among the citizens of cities and rural areas. There are many theories and experiments that prove the advantage of the chosen method of exposure to seeds and plants in order to obtain the best result of the final product. One of these areas is the influence of low-intensity laser radiation on plants and their seeds. This article examines the effect obtained from such an impact. And in particular, the authors are talking about increasing the sugar content of root crops obtained from sugar beet seeds treated with low-intensity laser radiation.

Involvement of Photoprotective Compounds of a Phenolic Nature in the Response of Arabidopsis Thaliana Leaf Tissues to Low-Intensity Laser Radiation.

The influence of low-intensity laser radiation (LILR) on the changes in the content of anthocyanins, kaempferol, quercetin and their glycosides in the leaves of 5-week-old plants of Arabidopsis thaliana L. was studied by means of methods of high-performance liquid chromatography and gas chromatography mass spectrometry (GC-MS). It was found that in the leaves subjected to a stimulating He-Ne laser radiation dose (3.6Jcm-2 , continuous wave radiation, wavelength-632.8nm, exposure time-5min), the radiation induced an increase in the content of such compounds, the most significant one being in the case of anthocyanins (9 times). The present study also revealed an increase in the antioxidant potential of kaempferol, quercetin and their glycosides as a result of laser exposure. This increase was due to the preferential synthesis of compounds with a larger number of OH-groups on the phenyl ring. Thus, the content of quercetin, which has five OH-groups in its structure, increased almost by three times as compared to the control.

The influence of photodynamic effects on microbial adhesion and the choice of structural materials for relocation of dentures for patients with inflammatory periodontal diseases.

The search for new methods of treatment of inflammatory periodontal diseases in patients with removable prostheses is an urgent issue for dentists. Currently, positive results in the treatment of gingivitis and periodontitis are achieved using photodynamic therapy, which is based on the destruction of photosensitized microorganisms under the influence of low-intensity laser radiation in a short period of time. This article presents the results of a study of the effectiveness of the choice of structural materials for relocation of removable prostheses in patients with inflammatory diseases of the oral cavity in combination with photodynamic therapy.

DYNAMICS OF CHANGES IN TEMPERATURE PARAMETERS IN BIOLOGICAL TISSUES UNDER LASER RADIATION OF DIFFERENT WAVE LENGTHS

Background. The use of lasers is one of the promising directions in the development of therapy and surgery. The effect of low-intensity laser radiation (LR) in therapeutic doses activates regenerative processes in tissues, which allows stimulating regeneration processes due to the intensification of microcirculation of metabolic processes and also has an effect on neuro-reflex and humoral pain mechanisms and on pain receptors. There is a need to clearly establish the LR parameters that are important in the development and receipt of effective results of therapy. Diagnostics in vivo allows getting the opposite answer about the reaction of the body to radiation with different dose indicators, power density and wavelength without damaging the biological tissue.Objective. Since laser devices are used for many physiotherapeutic and diagnostic procedures, for the correct application of low-intensity LR, it is necessary to take into account all the structural features of the influence zone, as an example, human skin and the processes of interaction of this LR with layers of biological tissues. Under the influence of LR begins the course of a wide range of photophysical and photochemical changes, occurs the temperature rise on the surface of the irradiated skin and physicochemical transformations. The main purpose of the work was to determine the dynamics of changes in the temperature of the skin under the influence of LR.Methods. It was justified the use of mathematical dependence to determine the required dose of LR and a mathematical model for calculating the change in skin temperature under the influence of LR. An experiment was performed comparing the results of the calculation by mathematical model and the results of the experiment on the LR influence with wavelengths of 0.66 μm, 0.63 μm, and 0.46 μm on the nature and dynamics of temperature processes in the upper layers of the skin.Results. As a general result, data on the dynamics of changes in the temperature of the skin under the influence of LR are obtained. The results of the experiments and their comparison with the calculations of the mathematical model showed that there is a dependence of the temperature change on the structure and parameters of biological tissue, the power density of the LR, the radiation dose and the exposure time.Conclusions.According to the results of the work, it was concluded that a higher intensity of heat removal occurs in the skin of living tissues. This is mainly due to the microcirculation of organic liquids and blood.

The use of low-intensity laser radiation in the treatment of occupational diseases of the peripheral nervous system

Introduction. Laser radiation, discovered in the mid-twentieth century, is actively used in modern medicine. Along with the effects of high-energy lasers, researchers are attracting more and more attention to the methods of low-intensity laser therapy in the treatment of various diseases of the peripheral nervous system. At the same time, there are very few works in which the effectiveness of low-intensity laser radiation action in occupational diseases of the nervous system would be assessed.The aim of study was searching for information on the modern understanding of the mechanisms of action of low-intensity laser radiation and on its use in the treatment of occupational diseases of the nervous system in Russian and foreign scientific literature.Materials and methods: The article presents an overview of available scientific Russian and foreign literary sources. Search and selection of sources was carried out using open databases PubMed and RSCI.Results. Low-intensity laser therapy has anti-inflammatory, analgesic, immunocorrective and reflexogenic effects. There are described cases of successful application of low-intensity laser radiation in the treatment of vibration disease, radicular and muscular tonic syndromes, carpal tunnel syndrome. At the same time, there are no works in which the influence of low-intensity laser radiation on the dynamics of professional polyneuropathy of the upper limbs would be assessed.Conclusions: The biological effect of low-intensity laser radiation is complex and many-sided. Low-intensity laser therapy has a wide spectrum of action on various pathogenesis pathways, it is a common method of treating diseases of the peripheral nervous system, including professional aetiology. The urgent task is to substantiate and develop a technique for using low-intensity laser radiation to treat patients with professional polyneuropathies of the upper limbs (as the most common occupational disease of the peripheral nervous system) and to evaluate its effectiveness during dynamic observation.

The influence of laser radiation of the infrared range with a wavelength of 1270 nm on mice Balb/cNude

The effects of low-intensity laser radiation (LILR) on the skin depend on the wavelength and density of the irradiation flux. Moreover, the vast receptor field of the skin facilitates the systemic influence of irradiation on the body. The objective of the present study was to evaluate the effects of low-intensity laser irradiation (LIRI) of the infrared range with a wavelength of 1270 nm on the skin of mice Balb/cNude. The study was carried out with the use of the linear immunodeficient mice Balb/c nude obtained from the animal house of the Pushchino branch of the Academicians M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Biorganic Chemistry. The animals were irradiated once with a fiber laser of stimulated Raman scattering with a wavelength (λ) of 1270 nm, a power of 1.96 mW during the 2 min exposure at an intensity of 10 mW/cm2, and an energy flux density of 1.2 J/cm2. The histological studies and morphometry of the skin autoptate were performed. In adition, the expression of microRNA-21, -31, -130a, -191, -200c, -205, -218 was determined in the skin. Reverse transcription and real-time PCR reactions with Taq-Man probes and primers were performed on the nucleic acid amplifier CFX96 ('BioRad', USA).MicroRNA-191 was chosen as the reference gene. The bioinformation analysis of signaling pathways involving the studied microRNAs was performed using the DJANA miRPath database v.3.0. A significant increase in the amount of keratinocytes of the basal layer was documented together with diffuse lymphoid-leukocyte infiltration of the interlobular connective tissue of the subcutaneous fat and endomysium after LILR. The pattern of microRNA expression was tissue-specific. An increase in the expression of micro-RNA-31 and-21 in the skin and a multidirectional change in miRNA-200 and -218 levels were shown. The bioinformation analysis showed that miR21 and miR31 were involved in the regulation of such signaling pathways as PI3K-Akt, Jak-STAT, MAPK, and mTOR of importance for carcinogenesis. Also, they have a signaling significance in the development of melanoma, kidney cancer, prostate cancer and malignant glioma. The data obtained in this study suggest activation of the tumor cell-specific and basic processes in the skin of immunodeficient Balb/cNude mice under the influence of low-intensity laser radiation with a wavelength of 1270 nm applied at a dose of 1.2 J/m2.

Influence of low-intensity laser radiation on the dynamics of some phytohormone content in the callus tissues of wheat Triticum aestivum L.

We carried out an analysis of the influence of low-intensity He–Ne laser radiation on the dynamics of changes observed in the content of the phytohormones abscisic, jasmonic, salicylic, and indoleacetic acids in wheat calluses of Triticum aestivum L. using chromatography–mass spectrometry. He–Ne laser irradiation (λ = 632.8 nm) in its stimulant dose (3.5 J cm−2) was proved to cause a decrease in jasmonic and indoleacetic acid content and, simultaneously, a significant (2.5–3 times) increase in ‘stress’ abscisic and salicylic acids. The response of jasmonic acid to He–Cd laser irradiation (λ = 441 nm) was specific, which is probably due to the blue-light-induced biosynthesis of the acid. This article discusses a possible connection between substantial changes in growth regulator content within the plant tissue under the influence of low-intensity laser irradiation and the stress character of this type of radiation.

The influence of low-intensity laser radiation on the functional activity of neutrophils in the patients presenting with community-acquired pneumonia

The objective of the present study was to elucidate the influence of low-intensity laser radiation on the results of the nitroblue tetrazolium (NBT) test in the patients presenting with community-acquired pneumonia. A total of 100 patients with community-acquired pneumonia were available for the examination of whom 70 were treated with intravenous low-intensity laser irradiation of blood (ILIB) by means of the ILIB-405 technique during 7 days. The functional activity of neutrophils was estimated from their ability to reduce nitroblue tetrazolium in both spontaneous and stimulated NBT-tests. The analysis of the data obtained in the study has demonstrated the significant improvement of the results of the NBT tests in the group of patients receiving the ILIB treatments regardless of whether its content was originally elevated or reduced. The inclusion of intravenous low-intensity laser irradiation of blood in the combined treatment of the patients with community-acquired pneumonia appreciably promotes normalization of the bactericidal activity of neutrophils.

The effect of continuous low-intensity laser irradiation of the red spectrum on the changes in the functional activity and speed of NADPH-oxidase response of human peripheral blood neutrophils

Registration of the changes in the functional metabolic status of neutrophils under the influence of low-intensity laser radiation (LILR) can be useful for the choice of the exposure parameters in the studies designed to evaluate immunotropic effects. Such investigations, in their turn, are very promising in clinical terms since their results can be used to optimize the laser therapy techniques. The objective of the present study was to evaluate the degranulation potential of neutrophils and the intensity of their NADPH-oxidase reaction in response to the exposure to laser radiation as exemplified by the release of lysosomal granules in vitro. The source of radiation was the LASMIK device operated in the continuous mode at the following parameters: wavelength 635 nm, power density 0.12 mW/cm2, exposure time 10, 30, 90, 120, 150 s in the case of the automatic timer control and 100 s in the case of manual shutdown. It was shown that the maximum lysosomal activity and release of lysosomal granules took place at a 90-10 s exposure and a wavelength of 635 nm with the appearance of a plateau within 120 and 150 s after the onset of irradiation. In the case of a shorter exposure of neutrophil granulocytes to LILR (10 and 30 s) no pronounced effect was observed. It means that low-intensity laser radiation of the red spectrum with a wavelength of 635 nm is a physical stimulus reinforcing exocytosis of lysosomal granules by neutrophils in vitro. The reliable changes of NADPH-oxidase activity of neutrophil granulocytes isolated from donor peripheral blood are recorded at an optimum exposure time of 90-100 s. It is concluded that the laser therapy techniques, at least those designed to regulate neutrophils should be applied with the optimum exposure time of 90-100 s.

Effect of GaAIAs Laser on Reactional Dentinogenesis Induction in Human Teeth

This study investigated the biomodulatory effect of the gallium- aluminum-arsenate laser (GaAlAs) in pulp cells on reactional dentinogenesis, and on the expression of collagen type III (Col III), tenascin (TN), and fibronectin (FN) in irradiated dental tissues and controls (not irradiated). Several studies suggest a biomodulatory influence of low-intensity laser radiation in the inflammatory and reparative processes of biological tissues. Sixteen human premolar teeth were selected (after extraction due to orthodontal reasons) and divided into irradiated and control groups. Black class V cavity preparations were accomplished in both groups. For the irradiated group, GaAlAs laser (670 nm, 50 mW) with an energy density of 4 J/cm2 was used. Soon after, the cavities were restored with a glass ionomer and the extractions made after 14 and 42 days. Histological changes were observed by light microscopy; less intense inflammatory reaction in the irradiated group was found when compared to the controls. Only the irradiated group of 42 days exhibited an area associated with reactional dentinogenesis. After immunohistochemical analysis by the streptoavidin-biotin complex (SABC) method, the expression of Col III, TN, and FN was greater in the irradiated groups. Our results suggest that a GaAlAs laser with energy density of 4 J/cm2 and wavelength of 670 nm caused biomodulation in pulp cells and expression of collagen, but not collagen of the extracellular matrix, after preparation of a cavity.

Influence of Low-Intensity Laser Radiation on the Rheological Characteristics of Human Blood

The influence of low-intensity laser radiation (LLR) on the rheological characteristics of human blood has been investigated. The results of the investigation of the blood velocity in the human skin capillaries, the erythrocyte aggregation time, and the spectral width of scattering intensity fluctuations depending on the irradiation time are presented. It has been shown that laser irradiation leads to a decrease in the degree of aggregation of erythrocytes and, accordingly, to an increase in the velocity of blood microcirculation, which can be used for laser therapy and diagnostics and treatment of cardiovascular diseases (CVD). To explain the results obtained, a method of the LLR–biotissue interaction is proposed.

Influence of low-intensity laser radiation on biogenic amines and clinical and biochemical parameters in viral hepatitis

In recent years, much attention has been paid to the development of new drug-free methods of treating many diseases in various fields of medicine. Since the 80s, low-intensity lasers have been increasingly introduced into the clinic of internal medicine.