With the purpose of enhancing oil production, the present work was carried out to elucidate relationships between photosynthesis of leaves, siliques and seeds yield and seeds oil accumulation of oilseed rape. Field trials, in which two repeated experiments was carried out during 2012–2014 growth season, a rape hybrids the “Qin You No.7” (Brassica napus L.) variety was taken into account. The results showed that, on rape plant the photosynthetic capacity and chlorophyll a, b, carotenoid contents of leaf were significantly higher than that of silique shell. Oil content of 94.7% was achieved in young seeds (sampled at 25th day after flowering ending stage of the tested rape plant) versus to mature seeds, saturated fatty acids percent was higher whereas oleic acid percent was lower of oil extracted from young seeds. During flowering period of the rape plants tested, area and dry weight of leaves attained maximum, treatments of removing leaves induced reduction in seeds number per silique, siliques number, seeds yield per plant and seeds oil content, these indexes were respectively decreased by 73.6%, 43.4%, 83.4% and 10.5% in maximum, and seeds oil composition was not significantly influenced; during seeds growing period of the tested plants, surface area and dry weight of siliques attained maximum, under shading siliques treatment, the 1000-seed weight, seeds yield per plant and seeds oil content were respectively reduced by 57.5%, 61.4% and 44.7% in maximum, and seeds oil oleic acid (C18:1) and linolenic acid (C18:3) percent was decreased, linolic acid (C18:2) and erucidic acid (C22:1) percent was increased. So for oilseed rape plant during flowering period, surface area and photosynthesis of leaves dramatically influenced siliques number, seeds number and seeds yield; while in seeds growth period, surface area and photosynthesis of siliques greatly influenced 1000-seed yield, seeds yield, seeds oil content and oil composition; oil accumulation in rape seeds initiated early since seeds commencing growth, seed mature degree influenced oil composition of seeds.