Influence Of Er Research Articles

Direct evidence of eddy breaking and tilting by edge sheared flows observed in the TEXTOR tokamak

In this letter, we present direct evidence of eddy breaking and tilting events observed at the edge of the TEXTOR tokamak using a 2D gas puff imaging diagnostic. The occurrence of eddy breaking depends on the magnitude of the Er × B flow shearing rate. The results confirm the theoretical predictions about the influence of Er × B sheared flows in tilting and splitting turbulence eddies, and consequently, reducing the turbulent transport.

Influence of Er,Cr:YSGG laser irradiation on enamel caries prevention

The objective of this study was to evaluate the effects of chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser irradiation on the acid resistance of dental enamel. Forty human enamel samples were divided into four groups. They were manually irradiated with an Er,Cr:YSGG laser device (λ = 2.78 µm, 20 Hz, 20 s), in a scanning mode, with and without water cooling, according to the following parameters: Group 1: 0.25 W, 62.5 J/cm(2), no water cooling; group 2: 0.25 W, 62.5 J/cm(2), 5.0 ml/min; group 3: 0.5 W, 125 J/cm(2), no water cooling; group 4: 0.5 W, 125 J/cm(2), 5.0 ml/min. No airflow was used. Afterwards, the samples were submitted to an acid challenge and assessed by cross-sectional Knoop microhardness at different depths (20, 40, 60, 80, and 100 µm) from the outer enamel surface. Average values were obtained for both irradiated and control areas in each sample and they were compared to obtain a percentage of microhardness increase. Data were analyzed by analysis of variance and Fisher's exact test (α = 5%). The percentage of microhardness increase observed in group 1 (+23.58%) was similar to group 3 (+19.12%), but higher than groups 2 (+3.61%) and 4 (10.9%) (p < 0.05). The comparison of the depths showed that the Er,Cr:YSGG laser acted in the superficial layers of the dental enamel. The findings of the present study suggest that the energy densities of 62.5 and 125 J/cm(2) were capable of increasing the acid resistance of human enamel. The presence of water during irradiation makes it difficult to obtain an enamel surface more resistant to acids.

Influence of Er,Cr:YSGG irradiation in prosthetic post space in the adhesion of fiber posts cemented with self-etching, total-etching or self-adhesive

Influence of Er,Cr:YSGG irradiation in prosthetic post space in the adhesion of fiber posts cemented with self-etching, total-etching or self-adhesive

Finite Element Analysis about the Properties of CFG-Pile Composite Foundation Based on Parametric Language PYTHON

As an international general finite element analysis software, ABAQUS has super nonlinear analysis function and is playing an increasingly important role in the numerical calculation analysis of geotechnical engineering structures. CAE module of its own provides a certain amount of convenience for the beginners, but it is inadequate in the face of more complex geotechnical engineering problems. Based on parametric language PYTHON, using its modular model code, bypassing the CAE module, the ABAQUS finite element analysis calculated and analyzed the influence of ER on the CFG pile composite foundation settlement and pile side friction. Finite element analysis and the results show that: the process of ABAQUS finite element analysis, which is based on the parameter language PYTHON, is simple and has very high computational efficiency and accuracy in the analysis complex geotechnical engineering problems.

Solidification behavior, microstructure and tensile properties of ZK60-Er magnesium alloys

ZK60-Er (erbium) alloys were made by melting ZK60 and Mg-Er magnesium alloys (20 wt.% Er) in an electric resistance furnace. The contents of Er were 0, 0.5, 1, 2, 3 wt.%, respectively. The influence of Er on solidification behavior, microstructure, corrosion resistant and mechanical properties of ZK60 magnesium alloy was studied. The results showed that long rod-like γ phase (ErZn 5) formed during solidification increased with increasing Er content in the range investigated, which resulted in the decrease of the amount of galvanic couplings between phase particles and alloy matrix and the marked improvement of corrosion resistant. It was also found that elongation of the alloys decreased with increasing Er content, but tensile strength of the alloys were improved by the addition of Er due to the strengthening effect of γ phases distributing along grain boundaries.

Influence of Er:YAG laser frequency on dentin caries removal capacity

The purposes of this study were to evaluate in vitro the influence of different frequencies of Er:YAG laser on the human dentin caries removal capacity. Thirty fragments obtained from third molars were randomly assigned into three groups (n = 10) according to the laser frequency used: 4, 6, and 10 Hz. The caries lesion (±1 mm deep) was induced before the irradiation by S.mutans cultures for 6 weeks. The specimens of all groups were irradiated with 200 mJ of energy in noncontact and focused mode under constant refrigeration (water flow: 2.5 mL/min). Quantitative analysis of the caries removal was performed by DIAGNOdent™ and the Axion Vision™ software. Qualitative analysis was performed by Scanning electron microscope (SEM) and light microscope (LM). Data were analyzed by ANOVA and Fishers' tests. The DIAGNOdent™ revealed that the caries removal was similar with 4 and 6 Hz and was superior with 10 Hz (P < 0.05). The analysis with Axion Vision™ software revealed that the caries removal was similar with 6 and 10 Hz and the 4 Hz group promoted the lowest caries removal. Through SEM morphologic analysis, some specimens irradiated with 4 Hz presented, under the demineralized dentin, a disorganized collagenous matrix. The LM images revealed that all frequencies used promoted irregular caries removal, being observed over preparations with 6 and 10 Hz. It can be concluded that the increase of Er:YAG laser frequency provided a higher dentin caries removal without selectivity to the disorganized dentin.

Influence of Er: Yag Laser on Shear Bond Strength of Self-etching Adhesives to Bovine Enamel: In vitro Study

ABSTRACT Purpose The aim of this study was to evaluate the influence of the enamel treatment with Er:YAG laser on the bond strength of self-etching adhesives. Materials and methods One-hundred bovine incisors were ground to obtain flat enamel surfaces. The bond area was delimited with 3 mm diameter adhesive tape. Specimens were divided in two groups: Group L: Enamel surface received Er:YAG laser application (Kavo KEY 3) at 300 mJ/6 Hz in sweeping mode for 30 seconds; Group N: Enamel surface received no additional treatment. Each group was divided into five subgroups according to self-etching adhesive: S&amp;E (Self &amp; Etch), FB (Futurabond NR), XE (Xeno III), OU (One-Up bond F), CL (Clearfil SE Bond). Cylinders of Z250 composite were fabricated on the bonding area using a teflon matrix. The teeth were stored in water at 37°C/ 24 hours and submitted to shear testing at a speed of 1 mm/min. Results The results were analyzed by two-way ANOVA and Tukey's test, obtaining p-value = 0.001. The mean values (±SD) in MPa for each group/subgroup were: N/FB—19.65(±3.87)a; N/CL—19.55(±3.78)a; N/OU—15.57(±2.85)ab; N/XE—15.32(±4.00)b; L/CL— 13.91(±2.54)b; L/OU—13.16(±2.17)bc; L/XE—9.74(±1.65)cd; N/S&amp;E—8.83(±2.63)d; L/FB—8.30(±2.27)d; L/S&amp;E—6.56(±1.55)d. Conclusion It was concluded that the enamel surface treatment with Er:YAG laser reduced the bond strength significantly for most of the self-etching adhesives tested.

Microtensile bond strength of resin composite to dentin treated with Er:YAG laser of bleached teeth

The objective of this study was to evaluate the influence of Er:YAG laser (λ = 2.94 μm) on microtensile bond strength (μTBS) and superficial morphology of bovine dentin bleached with 16% carbamide peroxide. Forty bovine teeth blocks (7 × 3 × 3 mm(3)) were randomly assigned to four groups: G1- bleaching and Er:YAG irradiation with energy density of 25.56 J/cm(2) (focused mode); G2 - bleaching; G3 - no-bleaching and Er:YAG irradiation (25.56 J/cm(2)); G4 - control, non-treated. G1 and G2 were bleached with 16% carbamide peroxide for 6 h during 21 days. Afterwards, all blocks were abraded with 320 to 600-grit abrasive papers to obtain flat standardized dentin surfaces. G1 and G3 were Er:YAG irradiated. Blocks were immediately restored with 4-mm-high composite resin (Adper Single Bond 2, Z-250-3 M/ESPE). After 24 h, the restored blocks (n = 9) were serially sectioned and trimmed to an hour-glass shape of approximately 1 mm(2) at the bonded interface area, and tested in tension in a universal testing machine (1 mm/ min). Failure mode was determined at a magnification of 100× using a stereomicroscope. One block of each group was selected for scanning electron microscope (SEM) analysis. μTBS data was analyzed by two-way ANOVA and Tukey test (α = 0.05). Mean bond strengths (SD) in MPa were: G1- 32.7 (5.9)(A); G2- 31.1 (6.3)(A); G3- 25.2 (8.3)(B); G4- 36.7 (9.9).(A) Groups with different uppercase letters were significantly different from each other (p < .05). Enamel bleaching procedure did not affect μTBS values for dentin adhesion. Er:YAG laser irradiation with 25.56 J/cm(2) prior to adhesive procedure of bleached teeth did not affect μTBS at dentin and promoted a dentin surface with no smear layer and opened dentin tubules observed under SEM. On the other hand, Er:YAG laser irradiation prior to adhesive procedure of non-bleached surface impaired μTBS compared to the control group.

Histological examination of experimentally infected root canals after preparation by Er:YAG laser irradiation

The influence of Er:YAG laser irradiation on periodontal tissues along the root surface and apical region during root canal preparation was histologically evaluated using experimentally infected root canals of rats. Eighty experimentally mesial infected root canals of mandibular first molars in rats were divided into four groups. In three groups, root canals were irradiated using an Er:YAG laser at 2 Hz with 34, 68, or 102 mJ/pulse for 30 s. Non-irradiated canals served as controls. The influence of laser irradiation on periodontal tissues along the root surface and apical area was evaluated histologically under light microscopy at 0 (immediately after), 1, 2, 4, and 8 weeks after irradiation. At all periods, no inflammation or resorption on the root surfaces caused by laser irradiation was observed in any cases in the control or 34 mJ/pulse-irradiated groups. However, mild to severe inflammation with resorption of root surfaces was observed in some cases in the 68- and 102-mJ/pulse-irradiated groups. No significant difference was apparent between control and laser-irradiated groups at the apical area for all experimental periods (p > 0.05). These results suggest that thermal influences on periodontal tissues of experimentally infected root canals during root canal preparation by Er:YAG laser irradiation are minimal if appropriate parameters are selected. Er:YAG laser irradiation is thus a potential therapy for human infected root canals.

The influence of ER on small HER2-positive invasive breast cancers.

1586 Background: There is controversy regarding the risk of recurrence of the node-negative, HER2-positive pT1a-b tumors. We have shown considerable variation in survival of HER2-positive tumors of all sizes. We now examine the HER2-positive breast cancers with regard to ER status, node-status, and tumor size. Methods: Using the California Cancer Registry 2000-2007, we studied 16,958 cases of stages 1-3, HER2-positive primary invasive breast cancer. Kaplan-Meier cause-specific 5-year overall survival (OS) was computed and the log-rank test was used to compare survival between node-positive and node-negative tumors within the T1a, T1b, T1c, and T2 categories. Cox-proportional hazards analysis was used to determine the effect of ER/PR/HER2 subtype within each tumor size category for the node-positive tumors after adjusting for age, race/ethnicity, stage, grade, and socioeconomic status. Anti-HER2 treatment information was not available. Hazard ratios (HR) and 95% confidence intervals were computed. Results: For all tumor sizes, survival was statistically significantly better for negative node-tumors than for node-positive tumors (p < 0.001). Node-negative T1a, T1b, and T1c tumors had 5-year survival of 99%, 98%, and 97% respectively. There were 7,430 node-positive cases and survival was 93% for T1a, 94% for T1b, and 90% for T1c. No variables were associated with risk of death for the T1a and T1b tumors. Compared with the ER-/PR-/HER2+ subtype (the molecularly defined HER2-positive subtype), the ER+/PR+/HER2+ (HR = 0.33; 95% CI = 0.22-0.49) and ER+/PR-/HER2+ (HR = 0.49; 95% CI = 0.30-0.81) subtypes were associated with a reduced risk of death for T1c and T2 tumors. Conclusions: It is not surprising that node-negative breast cancers have better survival than node-positive cases. We found that for HER2-positive, node-negative, T1a, T1b, and T1c tumors, 5-year OS is excellent and for node-positive cases, it is only slightly lower but still 90% or greater. For tumors less than 10mm, ER/PR/HER2 subtype does not appear to influence survival. For larger tumors, ER-positivity reduces the risk of death when compared with the molecularly defined HER2-positive subtype, suggesting that ER rather than HER2 has greater influence on OS. No significant financial relationships to disclose.

Shear strength of the bond to primary dentin: influence of Er:YAG laser irradiation distance

The aim of this study was to assess in vitro the influence of Er:YAG laser irradiation distance on the shear strength of the bond between an adhesive restorative system and primary dentin. A total of 60 crowns of primary molars were embedded in acrylic resin and mechanically ground to expose a flat dentin surface and were randomly assigned to six groups (n = 10). The control group was etched with 37% phosphoric acid. The remaining five groups were irradiated (80mJ, 2Hz) at different irradiation distances (11, 12, 16, 17 and 20mm), followed by acid etching. An adhesive agent (Single Bond) was applied to the bonding sites, and resin cylinders (Filtek Z250) were prepared. The shear bond strength tests were performed in a universal testing machine (0.5mm/min). Data were submitted to statistical analysis using one-way ANOVA and the Kruskal-Wallis test (p<0.05). The mean shear bond strengths were: 7.32 ± 3.83, 5.07 ± 2.62, 6.49 ± 1.64, 7.71 ± 0.66, 7.33 ± 0.02, and 9.65 ± 2.41MPa in the control group and the groups irradiated at 11, 12, 16, 17, and 20mm, respectively. The differences between the bond strengths in groups II and IV and between the bond strengths in groups II and VI were statistically significant (p<0.05). Increasing the laser irradiation distance resulted in increasing shear strength of the bond to primary dentin.

Influence of Er:YAG laser on surface treatment of aged composite resin to repair restoration

The purpose of this study was to investigate the effect of Er:YAG laser on surface treatment to the bond strength of repaired composite resin after aged. Sixty specimens (n = 10) were made with composite resin (Z250, 3M) and thermocycled with 500 cycles, oscillating between 5 to 55°C. The specimens were randomly separated in six groups which suffered the following superficial treatments: no treatment (GI, control), wearing with diamond bur (GII), sandblasted with aluminum oxide with 27.5 µm particles (GIII) for 10 s, 200 mJ Er:YAG laser (GIV), 300 mJ Er:YAG laser (GV), and 400 mJ Er:YAG laser (GVI), with the last 3 groups under a 10 Hz frequency for 10 s. Restoration repair was done using the same composite. The shear test was done into the Universal testing machine MTS-810. Analyzing the results through ANOVA and Tukey test, no significant differences were found (p-value is 0.5120). Average values analysis showed that superficial treatment with aluminum oxide presented the highest resistance to shear repair interface (8.91MPa) while 400 mJ Er:YAG laser presented the lowest (6.76 MPa). Fracture types analysis revealed that 90% suffered cohesive fractures to GIII. The Er:YAG laser used as superficial treatment of the aged composite resin before the repair showed similar results when used diamond bur and sandblasting with aluminum oxide particles.

Simulations of ITER with combined effects of internal and edge transport barriers

Predictive simulations of ITER with the presence of both an edge transport barrier (ETB) and an internal transport barrier (ITB) are carried out using the BALDUR integrated predictive modelling code. In these simulations, the boundary is taken at the top of the pedestal, where the pedestal values are described using theory-based pedestal models. These pedestal temperature models are based on three different pedestal width scalings: magnetic and flow shear stabilization (Δ ∝ ρis2), flow shear stabilization () and normalized poloidal pressure (). The pedestal width scalings are combined with a pedestal pressure gradient scaling based on the ballooning mode limit to predict the pedestal temperature. A version of the semi-empirical Mixed Bohm/gyroBohm (Mixed B/gB) core transport model that includes ITB effects is used to compute the evolution of plasma profiles. In this model, the anomalous transport in the core is stabilized by the influence of Er × B flow shear and magnetic shear, which results in the formation of ITB. The combination of the Mixed B/gB core transport model with ITB effects, together with the pedestal model, is used to simulate the time evolution of plasma current, temperature, and density profiles for ITER standard type I ELMy H-mode discharges. It is found that ITER fusion performance using the BALDUR code with Mixed B/gB transport model without the presence of ITB is quite pessimistic (Fusion Q ∼ 3). The presence of ITB is crucial and can result in a significant improvement, which is needed for achieving a target Fusion Q of 10. The improvement due to the presence of ITB is almost the same for all simulations with those three pedestal temperature models. This is caused by the predicted pedestal temperature from each pedestal temperature model varying just slightly. The presence of ITB has a strong impact on both temperature profiles, especially near the centre of the plasma, but has a small impact on electron, deuterium, tritium and carbon density profiles, except the helium density profile. The formation of ITB does not impact on the pedestal. It is also found that during a sawtooth crash, the temperature profiles drop significantly, but there is a small change in the density profiles. However, the sawtooth oscillation has no impact on the pedestal. When the auxiliary heating power is turned off, it is found that significant fusion power is sustained.

Influence of Er:YAG Laser on Microhardness of Enamel Adjacent to Restorations Submitted to Cariogenic Challengein Situ

This study assessed the effect of the Er:YAG laser on enamel adjacent to restorations submitted to cariogenic challenge in situ, by microhardness analysis. The influence of Er:YAG laser energy on secondary caries formation has been investigated in vitro. However, no research involving demineralization around cavities prepared with the Er:YAG laser under intra-oral conditions has yet been described. Slabs of enamel were randomly assigned to seven groups (n = 12), according to the cavity preparation technique: groups I, II, and III--Er:YAG laser, 250 mJ, at 2, 3, and 4 Hz, respectively; groups IV, V, and VI--350 mJ, at 2, 3, and 4 Hz, respectively; group VII--high-speed handpiece (control). Cavities were restored and the specimens were fixed in intra-oral appliances and worn by 12 volunteers for 14 d. Sucrose solution was applied to each slab 6 times/d. Samples were removed, sectioned, and examined for microhardness at 100, 200, and 300 microm (factor distance), and 30, 60, and 90 microm (factor depth), from the restoration and enamel surface, respectively. Analysis of variance according to a split-plot model showed no difference among the cavity preparation techniques, among distances, or among depths, as well as no difference in the interaction between the factors of cavity preparation and distance. A significant difference was found in the interaction of cavity preparation and depth (p < 0.0001), as identified by contrast technique. The results revealed that the Er:YAG laser did not differ from conventional cavity preparation with regard to enamel microhardness.

Influence of Er,Cr:YSGG Laser Treatment on Microtensile Bond Strength of Adhesives to Enamel

The current trend towards minimum-intervention dentistry has introduced laser technology as an alternative technique for cavity preparation. This study assessed the null hypothesis that enamel prepared either by Er,Cr:YSGG laser or conventional diamond bur is equally receptive to adhesive procedures. The buccal and lingual surfaces of 35 sound human molars were prepared with Er,Cr:YSGG laser or a medium-grit diamond bur. One etch&rinse (OptiBond FL) and three self-etch adhesives (Adper Prompt L-Pop, Clearfil SE Bond and Clearfil S3 Bond) were applied on laser-irradiated and bur-cut enamel, followed by the application of a 5-6 mm build-up of Z100. The micro-tensile bond strength (microTBS) was determined after 24 hours of storage in water at 37 degrees C. Prepared enamel surfaces and failure patterns were evaluated using a stereomicroscope and a field-emission-gun scanning electron microscope (Feg-SEM). The pTBS to laser-irradiated enamel was significantly lower than to bur-cut enamel (p<0.05), with the exception of Clearfil S3 Bond, which bonded equally effectively to both substrates. The latter presented the highest microTBS on laser-irradiated enamel, though it was not statistically different from the microTBS of OptiBond FL. SEM analysis revealed significant morphological alterations of the laser-irradiated enamel surface, such as areas of melted and recrystalized hydroxyapatite and deep extensive micro-cracks. In conclusion, the bonding effectiveness of adhesives to laser-irradiated enamel depends not only on the structural substrate alterations induced by the laser, but also on the characteristics of the adhesive employed.

The Influence of Er:YAG Laser Conditioning versus Self-Etching Adhesives with Acid Etching on the Shear Bond Strength of Orthodontic Brackets

The aim of this study was to compare the shear bond strength (SBS) of orthodontic brackets after acid etching, laser ablation, self-etching primer application, and combined treatments. One hundred defect-free premolars were randomly assigned to five groups. The teeth received the following treatments: group 1: phosphoric acid + Transbond XT primer; group 2: Transbond Plus Self-Etching Primer (TPSEP); group 3: Er:YAG laser ablation + Transbond XT primer; group 4: Er:YAG laser ablation + phosphoric acid + Transbond XT primer; and group 5: Er:YAG laser ablation + TPSEP. Transbond XT Adhesive Paste was used in all groups for bracket bonding. Samples were stored in deionized water at 37 degrees C for 24 h. The shear debonding test was performed at a crosshead speed of 5 mm/min. Failed brackets were examined by a stereomicroscope at 16 x magnification to determine the bond failure interface using a modified adhesive remnant index (ARI). SBS values were compared using one-way analysis of variance (ANOVA). The Kruskal-Wallis test was used for ARI scores and pairwise comparisons were done using the Mann-Whitney U test with Bonferroni correction. Mean SBS values (MPa) and standard deviations for the groups were: group 1, 13.18 +/- 3.45; group 2, 13.52 +/- 4.38; group 3, 10.33 +/- 4.74; group 4, 13.00 +/- 3.47; and group 5, 12.46 +/- 6.31. No significant differences were observed in the SBS of all the groups evaluated, but there was a significant difference in ARI scores, with group 3 showing higher ARI scores than groups 1 and 4. Er:YAG laser ablation and self-etching primer application showed similar SBS values as conventional acidetching for bonding brackets.

Estrogen Receptor α Mediates Breast Cancer Cell Resistance to Paclitaxel through Inhibition of Apoptotic Cell Death

Estrogen receptors (ER) are expressed in approximately 65% of human breast cancer. Cumulative data from clinical trials and retrospective analyses suggest that some chemotherapeutic agents may be less effective in patients with ER-positive (ER+) tumors than those with ER-negative (ER-) tumors. Paclitaxel is an active agent used in breast cancer chemotherapy. To investigate the possible influence of ER on the therapeutic efficacy of paclitaxel and its underlying mechanism, we established several isogenic ER+ cell lines by stable transfection of ERalpha expression vectors into ER- breast cancer BCap37 cells. We showed that 17-beta estradiol significantly reduces the overall cytotoxicity of paclitaxel in BCap37-expressing ERalpha but has no influence on the ER- parental cells. Further analyses indicate that expression of ERalpha in BCap37 cells mainly interferes with paclitaxel-induced apoptotic cell death, without affecting paclitaxel-induced microtubule bundling and mitotic arrest. Moreover, we found that the addition of ICI 182,780 (Fulvestrant), a selective ER down-regulator, could completely reverse the resistance of ER+ BCap37 cells to paclitaxel. These findings showed that ERalpha-mediated breast tumor cell resistance to paclitaxel was through selective inhibition of paclitaxel-induced tumor cell apoptosis. Additionally, the combination of ICI 182,780 also sensitizes MCF-7 and T47D cell lines to the treatment of paclitaxel, which further confirmed the correlation between ERalpha and drug resistance in ER+ tumor cells. The results obtained from this study provide useful information for understanding ER-mediated resistance to paclitaxel and possibly other antineoplastic agents.

Microtensile bond strength of composite resin to human enamel prepared using erbium: Yttrium aluminum garnet laser

The Erbium: Yttrium Aluminum Garnet (YAG) laser used for preparation of cavity can alter the substrate and it could influence the bond strength of enamel. The aim of this in vitro study was to evaluate the influence of Er:YAG laser's energy using microtensile bond test. Three groups were obtained (cavity preparation) and each group was divided into two subgroups (adhesive system). After that the adhesive protocol was performed, sections with a cross-sectional area of 0.8 mm2 (+/-0.2 mm2) were obtained. The specimens were mounted in a universal testing machine (0.5 mm/min). Statistical analysis showed a decrease in bond strength for lased groups (p < 0.01) and when the total-etching adhesive system was used the laser 300 mJ subgroup showed higher bond strength compared to the laser 250 mJ (p < 0.01). It was concluded that the cavities prepared using laser appear less receptive to adhesive procedures than conventional bur-cut cavities.

Influence of Er:YAG Laser on Cavity Preparation and Surface Treatment in Microleakage of Composite Resin Restorations

The purpose of this study was to assess microleakage on class V cavities prepared by Er:YAG laser with varying surface treatment. There has been little reported research on microleakage at cavities prepared and treated with Er:YAG laser. Fifty preparations using Er:YAG laser or a highspeed handpiece were developed. The surface treatment was performed as follows: Er:YAG laser (group 1); 35% phosphoric acid (group 2); laser + acid (group 3); finishing with low-speed + laser + acid (group 4); conventional preparation developed with a high-speed handpiece + acid (group 5--control). The samples were restored with Single Bond/Z250, thermocycled, isolated, and immersed in a 50% AgNO(3) solution. The restorations were sectioned, and the microleakage was measured in milimeters using a specific computer software. Data were analyzed employing ANOVAand Tukey test. Statistical analysis showed that group 5 (G5) obtained the lowest results of microleakage (22.05%), and it was statistically similar (p > 0.05) to G1 (37.7%) and different (p < 0.05) from the other groups (G2 = 50.4%; G3 = 43%; G4 = 44.2%). The occlusal margins (22.62%) demonstrated less microleakage (p < 0.05) than the cervical margins (56.32%). The use of Er:YAG laser for cavity preparation and surface treatment negatively influenced the marginal sealing of composite resin restorations.

Bone mass in young adults: relationship with gender, weight and genetic factors

To determine the relationship of the bone mass attained in young adults with anthropometric and genetic factors. Cross-sectional study of normal individuals. We studied 341 healthy subjects between 22 and 45 years of age. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DXA) and correlated with body weight, height and nine polymorphisms in six genes involved in sex steroid metabolism (17-hydroxylase, aromatase and 5-reductase) and activity (oestrogen receptors (ER)-alpha and -beta, and androgen receptor). The BMD was higher in men than in women (spine: 1.048 +/- 0.120 vs. 1.034 +/- 0.112; hip: 0.907 +/- 0.131 vs. 0.822 +/- 0.104 g cm(-2), P < 0.001). However, the difference was due, at least in part, to the larger body size in men and diminished markedly after height adjustment. There was a negative correlation between age and hip BMD. Body weight was the single most influential factor on spine and hip BMD in both sexes, explaining 8-9% of BMD variance. Amongst the genetic factors studied, a common CA repeat polymorphism in ER-beta showed a significant association with BMD in women (P = 0.03 at the spine, and 0.008 at the hip). The relationship between ER-beta genotype and BMD persisted after adjustment by body weight and age, explaining a further 2-3% of BMD variance. Allelic variants of other genes studied were not related with BMD. Body weight and allelic variants of ER-beta are associated with BMD in young adults.