Influence Of Dimethyl Sulfoxide Research Articles

Influence of dimethyl sulfoxide on membrane fluidity under rapid supercooling

Influence of dimethyl sulfoxide on membrane fluidity under rapid supercooling

DMSO and Its Role in Differentiation Impact Efficacy of Human Adenovirus (HAdV) Infection in HepaRG Cells.

Differentiated HepaRG cells are popular in vitro cell models for hepatotoxicity studies. Their differentiation is usually supported by the addition of dimethyl sulfoxide (DMSO), an amphipathic solvent widely used in biomedicine, for example, in potential novel therapeutic drugs and cryopreservation of oocytes. Recent studies have demonstrated drastic effects, especially on epigenetics and extracellular matrix composition, induced by DMSO, making its postulated inert character doubtful. In this work, the influence of DMSO and DMSO-mediated modulation of differentiation on human adenovirus (HAdV) infection of HepaRG cells was investigated. We observed an increase in infectivity of HepaRG cells by HAdVs in the presence of 1% DMSO. However, this effect was dependent on the type of medium used for cell cultivation, as cells in William's E medium showed significantly stronger effects compared with those cultivated in DMEM. Using different DMSO concentrations, we proved that the impact of DMSO on infectability was dose-dependent. Infection of cells with a replication-deficient HAdV type demonstrated that the mode of action of DMSO was based on viral entry rather than on viral replication. Taken together, these results highlight the strong influence of the used cell-culture medium on the performed experiments as well as the impact of DMSO on infectivity of HepaRG cells by HAdVs. As this solvent is widely used in cell culture, those effects must be considered, especially in screening of new antiviral compounds.

대목별 DMSO (Dimethyl sulfoxide) 및 IBA (Indole-3 butyric acid) 처리농도가 포도 ‘흑보석’ 포트묘 생산에 미치는 영향

대목별 DMSO (Dimethyl sulfoxide) 및 IBA (Indole-3 butyric acid) 처리농도가 포도 ‘흑보석’ 포트묘 생산에 미치는 영향

The influence of dimethyl sulfoxide on resin–dentin bonding: A systematic review

This article aims to systematically review published literature about the influence of dimethyl sulfoxide (DMSO) on resin–dentin bonding. Online databases (Medline (PubMed), Embase, Web of Science, and the Cochrane Library) were searched on this topic. Gray literature was searched using Google Scholar. Published works until December 2020 were collected, and pertinent articles were selected in accordance with the eligibility criteria. Quality assessment was conducted via the modified Consolidated Standards of Reporting Trials (CONSORT) checklist and the modified Cochrane risk of bias tool. Thirty-eight studies were identified, twelve of which satisfied the eligibility criteria were included in this review. Micro tensile bond strength (MTBS) was set as the main outcome. Secondary outcomes include nanoleakage evaluation, the effect of DMSO on dentin collagen fibrils, and protease inhibition. Several studies proved the promotion of DMSO on long-term dentin bond strength of etch-and-rinse adhesives. However, evidence about its effect on bond strength of self-etch adhesives was insufficient. Different concentrations of DMSO solution also cause diverse impact on dentin bonds. Based on the included studies, DMSO considerably affects resin–dentin bonds by dissociating demineralized dentin matrix, inhibiting matrix metalloproteinases (MMPs), and enhancing penetration of resin monomers. The influence of DMSO on dentin bonding may offer inspirations for dental clinical practice to optimize resin–dentin bonds. • DMSO is beneficial for improving the long-term dentin bond strength of etch-and-rinse adhesives. • The influence of DMSO on self-etch adhesives remains controversial and needs more validation. • DMSO can dissociate demineralized dentin matrix, inhibit matrix metalloproteinases, and enhance resin monomer penetration. • DMSO may enlighten the concept of “wet bonding” technique from surface moisture to bio-modification of dentin. • DMSO offers inspiration for dental clinical practice to optimize resin–dentin bonds.

Influence of Dimethyl Sulfoxide and H2O on Hantzsch Reaction and Its Application in Pharmaceutical Analysis

Influence of Dimethyl Sulfoxide and H2O on Hantzsch Reaction and Its Application in Pharmaceutical Analysis

Influence of Dimethyl Sulfoxide on the Structural Topology during Crystallization of PbI2.

Hybrid metal-organic halides are an exciting class of materials that offer the opportunity to examine how fundamental aspects of chemical bonding can influence the structural topology. In this work, we describe how solvent adducts of lead halides can influence the crystallization and subsequent annealing of these hybrid phases. While the size and shape of organic molecules are known to govern the final topology of the hybrid, we show that the affinity of solvent molecules for Pb ions may also play a previously underappreciated role.

Influence of Dimethylsulfoxide and Dioxygen in the Fructose Conversion to 5-Hydroxymethylfurfural Mediated by Glycerol's Acidic Carbon.

Both the catalytic production of 5-hydroxymethylfurfural (5-HMF) from carbohydrates and the use of a catalyst obtained from residues stand out for adding value to by-products and wastes. These processes contribute to the circular economy. In this work it was evaluated optimized conditions for 5-HMF production from fructose with high yield and selectivity. The reaction was catalyzed by an acidic carbon obtained from glycerol, a byproduct of the biodiesel industry. Special attention has been given to the use of dimethyl sulfoxide (DMSO) as a solvent and its influence on system activity, both in the presence and absence of O2. Glycerol's carbon with acidic properties can be effectively used as catalyst in fructose dehydration, allowed achieving conversions close to 100% with 5-HMF selectivities higher than 90%. The catalyst can be reused in consecutive batch runs. The influence of DMSO in the presence of O2 should be considered in the catalytic activity, as the stabilization of a reaction intermediate by the [O2:DMSO] complex is favored and, both fructose conversion and 5-HMF yield increase.

The Influence of Dimethyl Sulfoxide as Electrolyte Additive on Anodic Dissolution of Alkaline Zinc-Air Flow Battery

The present work describes the effects of dimethyl sulfoxide (DMSO) in KOH aqueous electrolyte on the performance of a zinc-air flow battery. Aqueous electrolytes containing 7 M KOH and (0 to 20)% v/v DMSO were studied revealing a critical role of DMSO on the dissolution and deposition of zinc. The anodic zinc dissolution process was studied via cyclic voltammetry, Tafel polarization and electrochemical impedance spectroscopy (EIS). The presence of DMSO showed improved zinc dissolution performance with the highest peak of zinc dissolution being the electrolyte containing 5% v/v DMSO. Tafel analysis demonstrated a significant decrease in polarization resistance and an increase in corrosion rate due to the introduction of DMSO to the electrolyte. This suggests that DMSO has the ability to suspend zinc oxide in the electrolyte, thus preventing passivation of the zinc surface. EIS results revealed that by adding DMSO to the electrolyte, charge transfer resistance increased. This is attributed to the formation of passive layers having arisen from DMSO adsorption, the formation of zincate ions in the vicinity of the zinc surface, and the deposition of discharged products. A difference in Nyquist plots was observed for 20% v/v DMSO/KOH and 0% v/v DMSO/KOH electrolytes implying non-Debye relaxation behavior taking place due to the surface effects. The electrolytes were implemented in a zinc-air flow battery. Maximum power densities of 130 mW/cm2 (5% v/v DMSO) and 125 mW/cm2 (20% v/v DMSO) were obtained and were observed to be about 43% and 28% higher than that of the DMSO-free electrolyte. Results indicated that when 20% v/v DMSO was added to KOH solution, there was 67% zinc utilization efficiency (550 mAh/g) which provided 20% improvement in discharge capacity. Further, the battery with 20% v/v DMSO demonstrated excellent cyclability. Overall, DMSO shows great promise for enhancement of zinc dissolution/deposition in zinc-air batteries.

The influence of dimethyl sulfoxide (DMSO) on metabolic activity and morphology of melanoma cell line WM-266-4

Dimethyl sulphoxide (DMSO) is widely used as a solvent in biomedical research, regularly in concentrations up to 1%. Nevertheless, little is known about the effect of different DMSO concentration on WM-266-4 metastatic melanoma cells, which are often used in melanoma research. Due to resistance of melanoma cells high concentrations of cytotoxic substances soluble in DMSO are used in vitro tests. Consequently, total DMSO concentration often exceeds 1%. The aim of our study was to test the metabolic activity and morphology of WM-266-4 cells exposed to selected DMSO concentrations for different period of time. Cells were incubated in selected ethanol concentrations for comparison. MTT test was performed to determine proliferation activity of the cells and morphological analysis was carried out by phase-contrast microscopy. Our results show inhibitory effect of DMSO on WM-266-4 cells' metabolic activity. Morphology of the cells changed progressively with the time of exposure. Ethanol showed little effect on metabolic activity of the cells and no effect on cell morphology after selected period of time. According to our study, for specific in vitro tests concentrations of DMSO up to 1.5% may be appropriate for WM-266-4 cell line experiments.

Influence of Dimethyl Sulfoxide on the Low-Temperature Behavior of Cholesterol-Loaded Palmitoyl-oleyl-phosphatidylcholine Membranes.

The properties of lipid membranes at low temperature are important for a number of biomedical and biotechnological applications, and the success of these applications depends on understanding the effects of temperature changes on intermolecular lipid-lipid and lipid-water interactions. Here, we use Fourier transform infrared spectroscopy to study lipid suspensions in water/dimethyl sulfoxide (DMSO) solutions in the -60 to 30 °C range. DMSO is a cryopreservative agent of cellular systems, and its action is largely related to its interaction with the lipid membrane, especially in the low-temperature regime. In the present work, we analyze the effects of solvent composition on the structural and thermotropic properties of cholesterol (chol)-loaded liposomes of palmitoyl-oleylphosphatidylcholine (POPC) because POPC/chol liposomes are suitable models of the plasmatic membrane. To this extent, we compare the properties of lipid vesicles suspended in water and water/DMSO solution at 0.10 DMSO mole fraction and we observe that the gel phase of the membrane has an increased thermal stability on DMSO addition. We estimate that the amount of unfrozen water at T = -60 °C is much reduced by the presence of DMSO, both in the gel- and the liquid-ordered phase of the membrane. Interestingly, we also evidence a reduced hydration of the lipid heads in the presence of DMSO when the vesicles are dispersed in a liquid solution, whereas the addition of DMSO does not alter the hydration state of phosphate and carbonyl groups in the frozen state of the membrane.

The role of dimethyl sulfoxide (DMSO) in ex-vivo examination of human skin burn injury treatment

Dimethyl sulfoxide (DMSO) is one of the most versatile solvents in biological science, therefore it is frequently used as a solvent in biological studies and as a vehicle for drug therapy. DMSO readily penetrates, diffuses through biological membranes and ipso facto increases fluidity of liposomal membranes modelling stratum corneum. Thermal injury is associated with the appearance of lipid peroxidation products in the burned skin. The influence of DMSO on protein structure and stability is concentration and temperature dependant. The aim of this study was to assess the impact of DMSO on human burn wounds and examine the interactions between DMSO and skin surface. The real problem in burn treatment is hypoalbuminemia. At the level of the laboratory studies there was an attempt at answering the question of whether the DMSO will modify the standard serum solution. In the case of the incubation of skin fragments in 1%–100% DMSO, the following findings were reported: modification of the serum, appearance of low molecular weight oligomer bands, disappearance of albumin bands or reconstruction of native serum bands during incubation in antioxidant solutions. The result of the modification is also the exposure of FTIR 1603 and 1046cm−1 bands observed in frozen serum solutions. In the case of modification of the burned skin by DMSO solutions or antioxidants – frequency shifts, an increase in the intensity of amide I band as well as the appearance of the 1601cm−1 band can be specific biomarkers of the tissue regeneration process. In this study the areas 1780–1580cm−1 and 1418–1250cm−1 on the Raman spectra are particularly rich in spectral information.

Development of Methods for Safe Application of Viral Vectors for GeneEngineering Manipulations

Additional oncogene copy was transferred in normal cells, and additional tumor-suppression gene copy-in malignant cells, respectively, by transfection with the appropriate recombinant DNA-constructs, containing each one the respective gene. The results obtained were compared with the data from insertion of copy from the respective gene in prokaryotic bacteria strains E. coli, by analogical transfection with appropriate gene constructs. In application of low initial infections (high initial dilutions of viral suspensions, respectively) of eventually attenuated by many passages vaccine strains from the heterologous for mammals and mammalian cells avian viral species, only slight ultra-structural changes and signs of partially-formed immature viral particles in the cells, were established, but no cytopathic effect (CPE was observed). In freezing of the so inoculated mammalian cells after addition of cryoprotector Dimethylsulfoxide (DMSO), thawing and re-incubation in fresh cultivation medium, signs of activated cell proliferation were noted. One of the possible explanations could be eventual transfer of nucleotide sequences from viral particles to separate cells because of activated fusion processes on the influence of DMSO in drastic temperature changes. The results obtained proposed a possibility about application of the described methods as available alternatives for both vaccine production and gene-engineering manipulations with cells, compared with the procedures of preparation and application of designed quite expensive recombinant DNA-constructs. Future studies are necessary in this direction.

The influence of DMSO and ether via fast-dipping treatment for a perovskite solar cell

Preparing dense organic-inorganic hybrid perovskite film is hindered by the extremely low solubility of lead(II) iodide (PbI2) at one-step coating process using one-solution (PbI2 + CH3NH3I + DMF). Thus, dimethyl sulfoxide (DMSO) has been widely used to enhance the solubility through the formation of adducts. However, the required mixture of DMSO and DMF cannot be simultaneously evaporated at low temperatures, owing to the different boiling points, which are influenced by the surface morphology. Consequently, this also affects the solar energy conversion efficiency of the perovskite solar cells. In this study, we develop a new approach for controlling the evaporation of DMF and DMSO by dip-coating ether on the top of the prepared thin film composed of PbI2, methylammonium iodide (MAI), DMF, and DMSO. Interestingly, it reveals that ether acted as an agent for simultaneous evaporation at similar temperatures, which then allows it to control surface morphology, and achieve dense perovskite. The fabricated inverted-perovskite PbI2 thin film solar cell shows that the efficiency reached up to 16.3% with high reproducibility. The ether-dipping post-treatment serves as a promising means for enhancing the efficiency of perovskite solar cells.

The influence of the main components of the ultraphonophoresis phytocomplex method on the release of the biologically active substances (an experimental study)

The present study was designed to investigate kinetics of flavonoidrelease from the working compositions containing a phytocomplex. The basic parameters of this processes during phonophoresis were determined in the model in vitro experiments. The study has demonstrated the dependence of the flavonoid release rate on their initial concentration in the working compositions and the influence of dimethylsulfoxide (as well as the main and auxiliary agents of the working composition) on the release of biologically active substances. The technological methods designed for the enhancement of the effectiveness of the phytocomplex phonophoresis technique are proposed.

Probing the replacement of water by dimethyl sulfoxide in the hydration shell of N-methylacetamide by FTIR-spectroscopy

The influence of dimethyl sulfoxide (DMSO) on the hydration of a peptide group has been explored by measuring the FTIR spectrum of N-methylacetamide in different mixtures of DMSO and D2O. An analysis of the amide I’ profile indicates two processes. For DMSO mole fractions between 0 and 0.5, the hydration shell on the side of the carbonyl group is systematically perturbed, which leads to a destabilization and eventual elimination of the hydrogen bonding between the carbonyl group and D2O. Upon further increasing the mole fraction of DMSO, water on the amide group side of the peptide is completely replaced by DMSO and the NH⇋ND exchange is significantly impeded. In mixtures with a very high fraction of DMSO, NMA with and without or weak hydrogen bonding to a DMSO molecule coexist with the latter configuration being clearly dominant in pure DMSO. Our results corroborate the recently debated notion that DMSO functions as a chaotropic cosolvent even at low fractions in that it exhibits preferential binding of water to the solute and by perturbing the stability of the peptide-water entity. They also reveal a rather fast exchange between different peptide-solvent complexes at low DMSO concentrations.

Change in the reactivity of N-vinylsuccinimide after complexation with dimethyl sulfoxide

The influence of dimethyl sulfoxide on the copolymerization kinetics and the reactivity parameters of N-vinylsuccinimide was studied. It was shown that, owing to the complexation of monomer molecules with dimethyl sulfoxide molecules, the relative activities of monomers are changed. The electron structures of N-vinylsuccinimide and dimethyl sulfoxide molecules and the molecule geometry was considered, and a complexation mechanism was proposed. The Alfrey–Price Q-e parameters of N-vinylsuccinimide during the complexation were analyzed. The causes of the change in the reactivity of monomer were revealed.

The influence of the main components of the ultraphonophoresis phytocomplex method on the release of the biologically active substances (an experimental study)

The present study was designed to investigate kinetics of flavonoidrelease from the working compositions containing a phytocomplex. The basic parameters of this processes during phonophoresis were determined in the model in vitro experiments. The study has demonstrated the dependence of the flavonoid release rate on their initial concentration in the working compositions and the influence of dimethylsulfoxide (as well as the main and auxiliary agents of the working composition) on the release of biologically active substances. The technological methods designed for the enhancement of the effectiveness of the phytocomplex phonophoresis technique are proposed.В результате исследования кинетики высвобождения флавоноидов из рабочих составов, содержащих фитокомплекс, при ультрафонофорезе в модельных опытах in vitro были определены основные параметры процесса, установлена зависимость скорости высвобождения флавоноидов от их исходной концентрации в рабочем составе, изучено влияние диметилсульфоксида и основообразующих веществ рабочего состава на высвобождение биологически активных веществ. Предложены технологические приемы для повышения эффективности метода ультрафонофореза фитокомплекса.

ChemInform Abstract: Ring‐Opening Reaction of Aziridines with Amines under the Influence of Dimethyl Sulfoxide.

AbstractThis 1,2‐diamine synthesis can be performed with a wide range of aziridines and amines under mild conditions and does not require a metal reagent.

The influence of DMSO on the formation and photoelectrochemical properties of CdS thin films by electrodeposition method

The homodispersed CdS nanoparticles were prepared on Sn-doped indium oxide substrates (ITO) to form smooth and uniform CdS thin films by electrodeposition method from a dimethyl sulfoxide (DMSO) solution containing cadmium chloride and sulfur. The structure and morphologies of samples were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The results indicate that DMSO played an important role in formation of CdS nanofilms by affecting the nucleation and growth of the CdS nanoparticles. So, a DMSO-assisted growth process was proposed as a plausible mechanism for the formation of smooth and uniform CdS nanofilms. According to the photoelectrochemical test, the CdS thin film prepared in 30 % DMSO + 70 % H2O system exhibited maximum photocurrent and open circuit potentials. This is because the deposited CdS nanoparticles had better dispersity on ITO, which facilitated the propagation and kinetic separation of photogenerated charges.

Influence of dimethyl sulfoxide used as a solvent on the physical properties and long-term dentin bonding of hydrophilic resins

ObjectiveTo examine the feasibility of dimethyl sulfoxide (DMSO) incorporation into relatively hydrophilic resins as a new potential method to improve the durability of resin–dentin bonds. MethodsSix experimental light-curing BisGMA/HEMA resins solvated in ethanol and DMSO with increasing concentrations of DMSO (0, 0.5, 1, 2, 4 and 10wt%) were prepared. The degree of conversion (DC) was evaluated by Fourier Transform Infrared Spectroscopy (n=8); water sorption (Wsp) and water solubility (Wso) were gravimetrically assessed (n=10); and flexural strength (FS) and elastic modulus (E) were determined by a three-point bending flexural test (n=10). Flat dentin surfaces on sound third molars (n=10/group) were bonded with resins containing 0, 2, 4 and 10wt% DMSO used as a two-step etch-and-rinse system. Dentin microtensile bond strength was determined at 24h and after two-year aging in artificial saliva at 37°C. ResultsDMSO significantly affected Wsp (p=0.0006), DC, Wso, FS, and E (p<0.0001). In general, the resins’ mechanical/physical properties were not affected by 2% or lower DMSO incorporation. Incorporation of 4% or higher DMSO content significantly increased DC, Wsp and Wso, but 2% or higher DMSO concentrations significantly reduced FS and E. No influence on immediate dentin bond strength occurred up to 4% DMSO incorporation. While 4% or higher DMSO concentrations impaired bond strength over time, the resin containing 2% DMSO presented significant higher dentin bond strength compared to the control resin after two year-aging. SignificanceThe use of DMSO as a new solvent in adhesive dentistry improves dentin bonding of relatively hydrophilic resins over time. 2% DMSO incorporation in BisGMA/HEMA resins should be sufficient to reduce bond strength loss without compromising polymer mechanical strength and physical properties.