Influence Of Culture Conditions Research Articles

The influence of cultivation conditions on the testate amoeba Arcella gibbosa: a perspective model for ecotoxicological studies

The influence of cultivation conditions on the testate amoeba Arcella gibbosa: a perspective model for ecotoxicological studies

Influence of Culture Conditions on Growth and Daidzein and Genistein Production in Hairy Root Cultures of Pueraria candollei var. mirifica

Influence of Culture Conditions on Growth and Daidzein and Genistein Production in Hairy Root Cultures of Pueraria candollei var. mirifica

Differential accumulation patterns of flavor compounds in Longjing 43 and Qunti fresh leaves and during processing responding to altitude changes

Variations in cultivars and cultivation altitudes have significant impacts on tea flavour compounds however lack of comprehensive understanding. This study provided insights into differential accumulation of crucial flavour compounds in response to cultivars, cultivation altitudes, and processing. Twelve flavonoids (262.4 ∼ 275.4 mg•g−1) and 20 amino acids (AAs) (56.5 ∼ 64.8 mg•g−1) were comparative analyzed in ‘Longjing 43′ and ‘Qunti’ fresh leaves harvested at low (80 m, LA) and high (500 m, HA) altitudes. Additionally, an in-depth correlation unravelling of 31 alkaloids, 25 fatty acids, 31 saccharides, 8 organic acids, and 7 vitamins and flavonoids/AAs during green tea (GT) and black tea (BT) processing was performed. Enhenced flavonoid accumulation alongside higher AAs and saccharides in HA GT promoted a sweet/mellow flavour. Abundant flavonoids, AAs, and saccharides derivates in LA BT gave rise to a sweet aftertaste. The study presents an integrated illustration of major flavour compounds' differential accumulation patterns and their interrelations, providing new insights into the influence of cultivation conditions on tea flavour.

The influence of cultivation conditions on the formation of psychoactive salvinorin A, salvinorin B, rosmarinic acid and caffeic acid in Coleus scutellarioides

Coleus scutellarioides (L.) Benth. is a popular species in the world, known for its characteristic magnificent colourful leaves. The study has revealed that the contents of rosmarinic acid and caffeic acid are significantly higher in the plant tissues cultivated in vivo than when under in vitro conditions. The performed qualitative and quantitative analyses confirmed the presence (whose averaged content) of salvinorin A (6.65 µg/1 g of fresh plant) and salvinorin B (50.46 µg/1 g of fresh plant) in tissues of Coleus scutellarioides (L.) Benth. of ‘Electric lime’ variety. The greatest quantities of these compounds were recorded for plants cultivated in vitro on the MS medium enriched with NAA (naphthyl-1-acetic acid) at a concentration of 0.5 mg∙ dm–3. The research detected differences in the amounts of compounds between plants grown in vivo and those cultivated in vitro. Addition of plant growth regulators into the breeding medium under in vitro conditions was found affecting the amounts of compounds in plant tissues.

Recycling of yeast multifunctional autolysates and extracts in the food industry

Recycling of industrial waste is one of the most crucial problems for the food industry. The forces of modern researchers are focused on solving this problem and using the biopotential of spent resources. Residual yeast from fermentation industries, in particular brewing, is of particular interest among the variety of food production waste. This is due to the extremely rich chemical composition of the yeast cell, and the possibility of obtaining a wide range of ingredients that exhibit both biological activity and technologically significant properties. Spent brewer's yeast is rich in proteins and carbohydrates, as well as vitamins B and minerals. The protein fraction, which accounts for 45–60% by dry weight (dw), contains all essential amino acids in sufficient quantities, which allows considering this secondary material resource an excellent source of protein with high biological value and a well-balanced AA profile. The carbohydrate fraction, comprising approximately 40% by dw, consists of intracellular carbohydrates (such as simple sugars and glycogen) as well as cell wall polysaccharides (such as β-glucan and α-mannan). Special emphasis is placed on the cell wall components due to their significant multidirectional biological activity and technologically important properties. Thus, β-glucan and α-mannan, along with emulsifying, sorbing and stabilizing properties, exhibit antioxidant and antimicrobial activity, immunomodulatory and prebiotic properties. This review presents an analysis of yeast autolysates and extracts sources as well as the influence of cultivation conditions and production methods on their chemical composition.

Effect of growth medium composition on the efficiency of non-ribosomal synthesis in bacteria of the genus Bacillus

Bacteria of the genus Bacillus are known for their ability to suppress a wide range of pathogenic microflora through the production of a variety of secondary metabolites, a significant proportion of which are non-ribosomal peptides. The importance of selecting conditions for the most efficient synthesis of non-ribosomal peptides is related to this. The influence of cultivation conditions can be assessed by analyzing the gene expression of non-ribosomal peptide synthetases of target NRPs and studying the mechanisms of its regulation. The aim of this study is to investigate the influence of growth medium composition on the expression of non-ribosomal peptide synthetase genes in Bacillus bacteria.

Influence of Cultivation Conditions on the Estimation Method of Soybean Community Height Using DSM with UAV Remote Sensing

Influence of Cultivation Conditions on the Estimation Method of Soybean Community Height Using DSM with UAV Remote Sensing

Influence of culture conditions on the secretome of mesenchymal stem cells derived from feline adipose tissue: Proteomics approach

This study aimed to describe the secretome of mesenchymal stem cells derived from feline adipose tissue (AD-MSCs) and compare the effects of different culture conditions on AD-MSC proteomics using a shotgun approach. Adipose tissue was collected from 5 female cats and prepared to culture. Conditioned media was collected at third passage, in which the cells were cultured under 4 conditions, normoxia with fetal bovine serum (N + FBS), hypoxia with FBS (H + FBS), normoxia without FBS (N – FBS), and hypoxia without FBS (H – FBS). Then, the secretome was concentrated and prepared for proteomic approaches. Secretomes cultured with FBS-free medium had more than twice identified proteins in comparison with the secretomes cultured with FBS. In contrast, hypoxic conditions did not increase protein amount and affected only a small proteome fraction. Relevant proteins were related to the extracellular matrix promoting environmental modulation, influencing cell signaling pathways, and providing a suitable environment for cell proliferation and maintenance. Moreover, other proteins were also related to cell adhesion, migration and morphogenesis. Culture conditions can influence protein abundance in AD-MSC secretome, and can give also more specificity to cell and cell-free treatments for different diseases.

Evaluation of the influence of the analytical characteristics of the microbiological blood study in bacteremia in a multidisciplinary hospital.

The culture method continues to be the "gold" standard for microbiological diagnosis of bloodstream infections. This is primarily due to the fact that the definition of the etiology of a generalized infectious process determines the etiotropic antibiotic therapy. To do this, it is necessary to conduct periodic microbiological monitoring of the prevailing microflora. To do this, in the present study, a retrospective analysis of the results of a microbiological blood test for sterility was performed in case of suspected bloodstream infections in a multidisciplinary hospital to assess the influence of analytical stage factors on the laboratory data obtained. Automatic hematological cultivators were used, identification was carried out based on the biochemical characteristics of microorganisms, as well as using time-of-flight mass spectrometry with matrix-activated laser desorption / ionization (MALDI-TOF MS). More than 10,000 research results were analyzed, the average microflora seeding rate was 15.1%. The analysis of the isolated microflora was carried out in 2 groups of positive results: at the beginning, the data obtained in the presence of growth in two vials at once were evaluated, then the positive results of blood cultures obtained in any one vial from a pair were studied. The predominance of gram-positive flora in the structure of microorganisms isolated from whole blood was revealed, the influence of cultivation conditions and the composition of thenutrient medium on the isolated flora was not found, however, a number of microorganisms, due to the specific characteristics of metabolism, were characterized by growth under strictly defined cultivation conditions. The presented study actualizes the need for constant microbiological monitoring in order to determine the prevailing hospital microflora, which can contribute to a timely response in order to limit the spread of highly virulent, aggressive, resistant strains of microorganisms leading to the development of generalized bloodstream infections.

Intraspecific Competition Results in Reduced Evenness of Tuber melanosporum Mating-Type Abundance from the Nursery Stage.

The highly prized black truffle is a fungus mostly harvested in orchards planted with mycorrhizal seedlings. It is an obligatory outcrossing fungus with a single MAT locus containing two alternative mating-type idiomorphs. In the orchards, at the mycorrhizal level, these mating types are frequently spatially segregated. Some studies found that this segregation was pronounced from the nursery stage, whereas others did not find such a marked segregation. Besides, information on the host tree species and nursery conditions used in Spain, one of the main truffle-producing countries, are very scarce. In this study, we investigated the temporal dynamics of mating types in nursery seedlings of Quercus ilex and Quercus faginea, as well as the influence of cultural conditions in the nursery. Our results indicated that at the plant level, there was a trend for one of the mating types to dominate over the other from the first to the second year in the nursery, in both host species and both nursery conditions tested. However, this segregation process was not so sharp as previously reported. Our results support the hypothesis that intraspecific competition results in reduced evenness of mating-type abundance from the nursery stage, although almost all seedlings maintained both mating types and, at the seedling batch scale, the occurrence of both mating types was roughly balanced.

Influence of Culture Conditions on Ex Vivo Expansion of T Lymphocytes and Their Function for Therapy: Current Insights and Open Questions.

Ex vivo expansion of T lymphocytes is a central process in the generation of cellular therapies targeted at tumors and other disease-relevant structures, which currently cannot be reached by established pharmaceuticals. The influence of culture conditions on T cell functions is, however, incompletely understood. In clinical applications of ex vivo expanded T cells, so far, a relatively classical standard cell culture methodology has been established. The expanded cells have been characterized in both preclinical models and clinical studies mainly using a therapeutic endpoint, for example antitumor response and cytotoxic function against cellular targets, whereas the influence of manipulations of T cells ex vivo including transduction and culture expansion has been studied to a much lesser detail, or in many contexts remains unknown. This includes the circulation behavior of expanded T cells after intravenous application, their intracellular metabolism and signal transduction, and their cytoskeletal (re)organization or their adhesion, migration, and subsequent intra-tissue differentiation. This review aims to provide an overview of established T cell expansion methodologies and address unanswered questions relating in vivo interaction of ex vivo expanded T cells for cellular therapy.

Комбинационная способность линий кукурузы и генетический контроль морфометрических параметров

The article presents the results of studying the combination ability of new dihaploid maize lines created by diploidization of haploid plants obtained using a haploinducer line. The experiment involved simple hybrids (30 combinations) obtained according to the complete diallel scheme of 6 homozygous lines. The effects of GCA and variance of SCA lines, as well as the components of genetic variance in terms of the main morphometric parameters of the plant (stem length, ear height, panicle length, stem diameter, plant leaf surface area) were revealed. Lines with a high GCA score and a dispersion of SCA have been identified, which are advisable to use in the breeding process for tall stature, resistance to lodging, and manufacturability. The influence of overdominance in the manifestation of the length of the panicle and the diameter of the stem and the predominance of additive effects in the formation of the height of the cob were noted. Analysis of the components made it possible to identify the number of genes or groups of genes that influenced the manifestation of traits in 2021 (stem length – 1–2, ear height – 2–3, panicle length – 1–2, stem diameter – 2–3, area of leaf surface – 1–2). Dominance according to the studied parameters is directed towards parental forms with a greater severity of the trait. A significant influence of cultivation conditions on the manifestation of the length of the stem, the height of the cob, the length of the panicle, and the diameter of the stem was noted.

THE INFLUENCE OF CULTIVATION CONDITIONS OF ALOE ARBORESCENS ON QUALITATIVE AND QUANTITATIVE COMPOSITIONS BIOLOGICALLY ACTIVE COMPOUNDS

Annotation: There are a number of medicinal plants on the Russian pharmaceutical market, which, despite their widespread use as official pharmacopoeial raw materials, do not have objective standardization methods. The composition of biologically active compounds (BAC) in these objects has not been fully studied, there is no information on the effect of cultivation conditions on the yield of various compounds. Aloe arborescens belongs to this raw material. Anthroquinone derivatives, polysaccharides, flavonoids were found in the leaves and lateral shoots of aloe. The presence of a predominant number of groups of malic and oxalic acids is also known. The actual problem is to evaluate the influence of cultivation conditions on the qualitative composition and quantitative content of organic acids in the raw material of Aloe arborescens. The aim of the study is to investigate the influence of cultivation conditions on the qualitative composition of organic acids and the quantitative yield of the sum of organic acids and polyphenolic compounds in Aloe arborescens leaves. Materials and methods: Objects: samples of raw materials made from the leaves of three plants of Aloe arborescens, distributed according to the method of care and the introduction of fertilizers; plant cultivation time was 4 weeks. To study the qualitative composition of the studied raw materials, the thin layer chromatography (TLC) method was used; samples of malic, citric and oxalic acids were used in the control. The study of the quantitative composition of organic acids and polyphenolic compounds in the studied samples was carried out using the methods of alkalimetric titration and UV spectrophotometry. Results: The investigation of the chemical composition of the aboveground organs of Aloe arborescens revealed the predominance of organic acids in the leaves, which leads to their active use in medicine. When studying the results of TLC, the largest amount of organic acids was found in the leaves of a plant grown under conditions of a reduced amount of watering, and the presence of malic, oxalic and citric acids in each of the samples of raw materials. The obtained results in quantitative analysis indicate the predominance of organic acids (1.24%) and polyphenolic compounds (5.37%) in the leaves of the plant cultivated by the soil drought method. Conclusion: it is advisable to use aloe leaves grown under soil drought conditions to obtain the maximum amount of biologically active compounds.

Influence of culture conditions on co-aggregation of probiotic yeast Saccharomyces boulardii with Candida spp. and their auto-aggregation.

Systemic infections caused by pathogenic Candida species pose a significant threat to public health in the past decades due to increasing resistance to existing antifungal drugs. Given this scenario, probiotics have been suggested as an alternative approach for managing Candida infections. Hence, the purpose of this study was to evaluate whether probiotic yeast Saccharomyces boulardii co-aggregate with Candida spp. as well as to determine their auto-aggregation ability in dependence on temperature (28°C, 37°C, 42°C) and pH (4.5, 7.0, 8.5) after 5h and 24h. Our results revealed that the aggregation of tested yeasts was lower in the first 5h but increased significantly after 24h. All strains were able to auto-aggregate in different degrees ranging from 47.46 to 95.95% assessed at 24h of incubation. Among them the highest auto-aggregation values had C. albicans and C. krusei strains followed by probiotic strain S. boulardii, while the less were observed in C. glabrata strains. In addition, co-aggregation between probiotic and Candida strains was strain-specific. It was evident that S. boulardii significantly inhibited the aggregation of C. albicans ATCC 10261, C. krusei ATCC 6258, and C. glabrata ZIM 2369. However, in C. glabrata ZIM 2382, the aggregation was even enhanced. Temperature and pH also affected the ability to aggregate in a different way only after 5h of incubation, with the highest cell aggregation evidenced at temperature 37°C in most cases and pH 4.5. These findings may be of importance when trying to establish probiotic use against pathogenic Candida species.

Influence of cultural conditions on comparative estimation of various phytocons-tituents and their effects on antibacterial activity of Euphorbia hirta L. leaves extracts

Influence of cultural conditions on comparative estimation of various phytocons-tituents and their effects on antibacterial activity of Euphorbia hirta L. leaves extracts

Influence of Cultivation Conditions on the Sioxanthin Content and Antioxidative Protection Effect of a Crude Extract from the Vegetative Mycelium of Salinispora tropica.

Due to their bioavailability, glycosylated carotenoids may have interesting biological effects. Sioxanthin, as a representative of this type of carotenoid, has been identified in marine actinomycetes of the genus Salinispora. This study evaluates, for the first time, the effect of cultivation temperature (T) and light intensity (LI) on the total cellular carotenoid content (TC), antioxidant activity (AA) and sioxanthin content (SX) of a crude extract (CE) from Salinispora tropica biomass in its vegetative state. Treatment-related differences in TC and SX values were statistically significantly and positively affected by T and LI, while AA was most significantly affected by T. In the S. tropica CE, TC correlated well (R2 = 0.823) with SX and somewhat less with AA (R2 = 0.777). A correlation between AA and SX was found to be less significant (R2 = 0.731). The most significant protective effect against oxidative stress was identified in the CE extracted from S. tropica biomass grown at the highest T and LI (CE-C), as was demonstrated using LNCaP and KYSE-30 human cell lines. The CE showed no cytotoxicity against LNCaP and KYSE-30 cell lines.

Influence of Cultivation pH on Composition, Diversity, and Metabolic Production in an In Vitro Human Intestinal Microbiota

Fecal microbiota transplantation, an alternative treatment method for gastrointestinal diseases, has a high recovery rate, but comes with disadvantages, such as high donor requirements and the low storability of stool. A solution to overcome these problems is the cultivation of an in vitro microbiota. However, the influence of cultivation conditions on the pH are yet unknown. In this study, the influence of the cultivation pH (6.0–7.0) on the system’s behavior and characteristics, including cell count, metabolism, and microbial composition, was investigated. With an increasing cultivation pH, an increase in cell count, total amount of SCFAs, acetate, propionate, and the abundance of Bacteroidetes and Verrucomicrobia were observed. For the concentration of butyrate and the abundance of Actinobacteria and Firmicutes, a decrease with increasing pH was determined. For the concentration of isovalerate, the abundance of Proteobacteria and diversity (richness and Shannon effective), no effect of the pH was observed. Health-promoting genera were more abundant at lower pH levels. When cultivating an in vitro microbiota, all investigated pH values created a diverse and stable system. Ultimately, therefore, the choice of pH creates significant differences in the established in vitro microbiota, but no clear recommendations for a special value can be made.

Influence of culture conditions on ellagitannase expression and fungal ellagitannin degradation

The role and mechanism of elagitannase is misunderstood because it exhibited different activities due to the low purity or complexity of substrates, and there is no available information about the biochemical, physicochemical and molecular characteristics of the enzyme. This study was aimed to obtain enzymatic extracts by Aspergillus niger GH1 in solid-state fermentation, using dextrose and ellagitannins as inducers of ellagitannase. Protein and bioinformatic analysis were performed to identify the protein sequence expressed in terms of culture conditions. The presence of ellagitannins increased ellagitannase activity 1143-fold compared to dextrose. The higher ellagitannase activity was found at 18 h of culture (1143.30 U g-1PE). Three groups of proteins were identified in both cultures: β-glucosidase, phospholipase C, and triacylglycerol lipase. However, only phospholipase C was overexpressed with ellagitannins as inducers, showing the most spontaneous reaction with punicalagin (ΔG −8.56). These results suggest that phospholipase could be involved in ellagitannins biosynthesis.

Optimization of Growth Medium and Physicochemical Parameters for High-Yield Biomass Production and Active Metabolites from Aspergillus terreus

The selection of a nutrient medium and optimization of physicochemical parameters play a crucial role in the growth enhancement and production of bioactive secondary metabolites of fungi. The current study was to evaluate the influence of cultural conditions and physicochemical parameters affecting the growth and biomass production of Aspergillus terreus. Nutrient medium ((Potato dextrose broth, Czapek’s Dox broth, Sabouraud dextrose broth, Potato Carrot broth and malt extract broth) in conjunction with physicochemical parameters such as temperature, pH, nitrogen sources, carbon sources, and salinity were assessed for optimal biomass production. The growth rate of the fungus was measured by determining the mycelial dry weight, and enhancement of antioxidant activity through DPPH scavenging potency in the culture broth. The highest growth rate of the fungus was observed in potato dextrose broth (PDB) compared to the other nutrient media at a significant level (p< 0.05). The present study demonstrated that PDB supplemented with cellulose and urea as carbon and nitrogen sources with a pH of 5.5 and 4% salinity at 28°C are the suitable conditions for maximal growth and biomass production. However, the antioxidants activity was at its peak level in PDA enriched with sucrose and ammonium sulphate at pH 5.5, and 7% salinity at 28°C. Thus, culture conditions, including nutrient components and physicochemical parameters play an important role in the growth rate A. terreus, to exploit the maximum yield for various biomedical applications.

Results of the research of onion in the annual culture in the conditions of the Priobskaya zone of the Altai Territory

Relevance. In the Western Siberia conditions bulb onion is grown mainly from onion sets. With this method of cultivation, large costs are spent on growing and storing the onion sets. In this regard, the cultivation of it through the seeds is of particular importance. This problem has not been studied enough in Priobskaya zone of Altai Territory conditions.Materials and methods. In our research, we studied the influence of cultivation conditions on the formation of economically valuable traits of varieties and a hybrid of bulb onion grown by sowing seeds in the ground in Priobskaya zone of Altai Territory. Three varieties were taken as objects of research: Odnoletniy Sibirskiy, Zolotnichok, Odintsovets, and one Candy F1 hybrid.Results. On average, over the years of research on early maturity, the standard Odnoletniy Sibirskiy variety (87-88 days) showed itself to be the most early maturing. Candy F1 hybrid can be used as a source of early green mass. Leaf regrowth was the earliest of all onion samples studied. The largest leaf apparatus in the conditions of Priobskaya zone of Altai Territory is formed by the varieties Odintsovets (4.1 pieces per plant) and Odnoletniy Sibirskiy (3.8 pieces per plant). The maximum bulb mass was formed by the Candy F1 hybrid (51.5 g). The Candy F1 hybrid also was distinguished by the highest yield in the group of the studied samples. The increase in total yield was 5.1 t/ha, marketable 2.4 t/ha in relation to the standard. The maximum marketability in the experience was in the standard (80.9%).