Background: Use of osseointegrated oral implants has been an excellent method for the replacement of missing teeth. Biofilm formation on oral implants can cause inflammation of peri-implant tissues, which can affect the long-term success of osseointegrated implants.Aims and Objectives: The aims and objectives of this study lie in the comparative evaluation of biofilm formation among five differently treated surfaces on titanium samples and to evaluate the difference in the delay of biofilm formation among various surfaces.Methodology: Six sets of polished titanium samples were blasted with sintered hydroxyapatite (HA) and titanium dioxide (TiO2). Another set of samples were blasted and later loaded with gentamicin drug by vacuum drying. The control group comprised plain polished and gentamicin drug-loaded samples. And, evaluation of the strains was done for biofilm. Bacterial adhesion was evaluated on time intervals of 0 h, 1 h, 4 h, 24 h, and 48 h.Results: Bacterial adhesion was sequentially increasing in the polished samples. Initial bacterial adhesion was more on surface-modified samples when compared to polished samples in the 1st hour. Bacterial adhesion was retarded in gentamicin-coated HA-blasted samples up to 24 h. Bacterial adhesion was considerably less on TiO2-blasted samples up to 48 h.Conclusion: Implant surface modified with TiO2 and gentamicin showed delayed biofilm formation even up to 48 h. Surface modification with HA has gained considerable osteoconductive surface, which is a boon for the production of future implants with less expense, however further studies are to be carried out to prove its efficacy.