European Journal of ImmunologyVolume 52, Issue 6 Back CoverFree Access Back cover story: Eur. J. Immunol. 6'22 First published: 02 June 2022 https://doi.org/10.1002/eji.202270067AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Our back cover illustrates endogenous ASC (red) and caspase-1 (green) protein that was immunocytochemically decorated in THP-1 macrophages after priming with a TLR3 agonist and stimulation with nigericin to activate the canonical Inflammasome. The image is taken from the article by Ghait et al. (pp. 907–923), where the authors show the assembly of Asc specks and the concomitant recruitment of pro-caspase-1 during assembly and arming of the Inflammasome in macrophages. ASC aggregation into distinct dense “specks” is readily visible, as is the presence of caspase-1 (yellow merged pseudocolor) in the spot. Recruitment of pro-caspase-1 to Asc-specks was severely impaired in CNPY3-deficient THP1 macrophages, indicating an essential role of the ER-resident chaperone CNPY3 in the assembly and activation of the inflammasome. Volume52, Issue6June 2022 RelatedInformation