Partial pulpotomy is a pulp therapy in which a portion of damaged and inflamed coronal pulp tissue is removed surgically. A 22year-old female suffering from profound caries defect in her mandibular second molar was referred to our clinic. Tooth responded within normal limits to electrical pulp tests. No history of spontaneous pain, mobility or tenderness to percussion was noted. Radiographic examination revealed an enlargement towards the periodontal ligament of the tooth. It was diagnosed as hyperplastic pulpitis. A local anesthetic without vasoconstrictor was applied to the tooth. MTA was placed over the amputated pulp tissue and covered temporarily with zinc oxide–eugenol cement. The patient was re-called after 24 hours to asses if there was any sense of postoperative pain caused by the treated tooth. No postoperative sensitivity was noted. The temporary restoration was removed under aseptic conditions; MTA was then covered with glass ionomer cement. In the same appointment, the tooth was restored with resin composite. Examinations 1 year after the treatment revealed success with the observance of normal function, aesthetics with a vital pulp and healthy surrounding periodontal structures. MTA may be useful as a substitute for calcium hydroxide in pulpotomy procedures of permanent molars with hyperplastic pulpitis.