In this paper we establish some theorems about the group of affine transformations on a Riemannian manifold. First we prove a decomposition theorem (Theorem 1) of the largest connected group of affine transformations on a simply connected complete Riemannian manifold, which corresponds to the decomposition theorem of de Rham [4] for the manifold. In the case of the largest group of isometries, a theorem of the same type is found in de Rham’s paper [4] in a weaker form. Using Theorem 1 we obtain a sufficient condition for an infinitesimal affine transformation to be a Killing vector field (Theorem 2). This result includes K. Yano’s theorem [13] which states that on a compact Riemannian manifold an infinitesimal affine transformation is always a Killing vector field. His proof of the theorem depends on an integral formula which is valid only for a compact manifold. Our method is quite different and is based on a result [11] of K. Nomizu.