Background: Cardiac amyloidosis manifests as a progressive infiltrative cardiomyopathy for which delayed diagnosis is common and predicts a poor prognosis. Additionally, right ventricular dysfunction has been a predictor of mortality in cardiac amyloidosis. Strain imaging has been shown to detect subclinical cardiac dysfunction in a range of cardiomyopathies. Aims: We sought to evaluate RV function in a cohort of AL-amyloid patients, to determine if additional evaluation of the right heart allows earlier detection than conventional assessment of left ventricular function. Method: 64 AL-amyloid patients were retrospectively analysed and compared to 31 healthy controls. RV systolic function was assessed using conventional parameters including TAPSE and s’ velocity, as well as 2-dimensional RV free wall strain. Results: RV free wall strain (28.1 ± 2.8 vs 22.1 ± 5.8%, p < 0.0001) and TAPSE (24.5 ± 3.7 vs 19.8 ± 4.7 mm, p < 0.0001) were reduced in amyloid patients; however TAPSE remained within normal limits. S’ was not significantly different between groups (11.4±2 vs 11.2±2.8 cm/s; p = NS). RV FWS correlated with LVEF, LV wall thickness and LV global longitudinal strain (GLS). However, RV GLS was significantly lower in AL patients with moderate to severe vs mild or normal LV wall thickness. Importantly, in 8 AL patients despite preserved LV GLS, a reduced RV strain was demonstrated (RV FWS <22%). RV FWS showed a higher sensitivity than both LV GLS and TAPSE. Conclusion: RV strain demonstrated subclinical RV dysfunction in amyloid patients in whom conventional parameters remained normal. Additionally, 28% of patients with preserved LV function (GLS >17%) showed evidence of RV dysfunction.