3D printing technology has started to be used in many different areas of our lives. In this study, the panels' flexural behaviors and vibration-damping capabilities produced in different infill patterns (Grid, Cross, and Tri-hexagon) and different infill ratios (0%, 20%, 40%, 60%, 80%, and 100%) were investigated. The specimen dimensions were 12.7 mm in width, 127 mm in length, and 3.2 mm in thickness. A biopolymer polylactic acid (PLA) filament with Ø 1.75 mm was used to produce test specimens. Fused Deposition Modeling is a modern additive manufacturing technique; however, optimization is needed to enhance the properties of 3D-printed parts. The flexural properties, as well as vibration-damping characteristics of the 3D-printed specimen, were evaluated experimentally. The results show that the infill pattern and ratio significantly affect flexural behavior and vibration-damping properties. The flexural tests showed that the highest flexural strength was obtained in the Tri-hexagon pattern, while the highest damping capability was obtained on Cross pattern specimens. Additionally, with the increasing infill ratio, the flexural modulus, and flexural strength increase. The specific strength value obtained for the 20% fill rate for all patterns was observed as maximum Best damping properties were obtained on a 40% infill ratio with all the different tested patterns. This study also showed that infill pattern and ratio design could improve the plastic parts' properties, and 3D printing provides an easy way to test the properties of different infill patterns and ratios.