ABSTRACT Radiological examinations have played a crucial role in the identification and management of COVID-19 patients. Therefore, knowledge and awareness of infection control among healthcare workers in radiology departments are important to prevent disease transmission. This study aimed to assess the knowledge and practice of infection control for COVID-19 among healthcare workers in radiology departments in Saudi Arabia. A cross-sectional, online questionnaire was administered among healthcare workers in radiology departments in Saudi Arabia in May 2020. The questionnaire consisted of demographic characteristics, profession, knowledge of infection control for COVID-19, and good practice of infection control measures for COVID-19 in radiology departments. A descriptive statistical analysis and chi-square test were performed using SPSS software. A total of 234 (91%) of healthcare workers replied that they have good knowledge about the precautions needed during the examination of positive COVID-19 cases in radiology departments, and 216 (84%) replied that they knew the necessary precautions when using portable X-ray machine. Moreover, 191 (>74%) of those surveyed agreed that wearing personal protective equipment and following the CDC sequence. There was significant association between profession and good clinical practices in radiology departments regarding COVID-19. Such knowledge could limit the spread of COVID-19 among the healthcare workers in radiology departments.