Toxoplasma gondii, the causative agent of toxoplasmosis, is carried by a range of intermediate hosts, including pigeons. Eating raw or undercooked pigeon meat, as is common in regions of China, can easily pass on a potentially fatal T. gondii infection. However, there are little data about the seroprevalence and genotypes of T. gondii infection in pigeons. In this study, a total of 963 pigeon blood samples were collected from Jilin Province, Liaoning Province, and Inner Mongolia Autonomous Region in China between August 2015 and December 2016. The overall seroprevalence of T. gondii was 10.80% (104/963), with 8.80% (41/466) in males and 12.68% (63/497) in females. The seroprevalence of T. gondii in different regions varied from 9.04% (17/188) in Jilin City to 13.16% (20/152) in Shenyang City, a relatively high prevalence. The seroprevalence of T. gondii in juvenile pigeons (6.38%, 18/282) was significantly lower than in adults (86/681, p < 0.05). These findings could provide useful data for the execution of effective control measures against T. gondii infection in pigeons and/or other hosts. More importantly, the findings also suggest that pigeons should not be eaten raw.