Infant Formula Milk Research Articles

Escherichia coli Can Eat DNA as an Excellent Nitrogen Source to Grow Quickly.

Is DNA or RNA a good nutrient? Although scientists have raised this question for dozens of years, few textbooks mention the nutritional role of nucleic acids. Paradoxically, mononucleotides are widely added to infant formula milk and animal feed. Interestingly, competent bacteria can bind and ingest extracellular DNA and even integrate it into their genome. These results prompt us to clarify whether bacteria can “eat” DNA as food. We found that Escherichia coli can grow well in the medium with DNA as carbon and nitrogen sources. More interestingly, in the presence of glucose and DNA, bacteria grew more rapidly, showing that bacteria can use DNA as an excellent nitrogen source. Surprisingly, the amount of DNA in the culture media decreased but its length remained unchanged, demonstrating that E. coli ingested long DNA directly. The gene expression study shows that E. coli mainly ingests DNA before digestion and digests it in the periplasm. Bifidobacterium bifidum can also use DNA as the nitrogen source for growth, but not efficiently as E. coli. This study is of great significance to study DNA metabolism and utilization in organisms. It also lays a foundation to understand the nutritional function of DNA in intestinal flora and human health.

The natural occurring RRR-α-Tocopherol and Synthetic α-Tocopherol stereoisomers in maternal plasma, cord plasma and breast milk among six regions of China.

The study aimed to investigate RRR-α-Tocopherol and synthetic α-Tocopherol stereoisomers in maternal plasma, cord plasma and breast milk from different regions of China, providing a reference for further guidance on maternal diet and the potential need to supplement mothers with vitamin E. Two hundred and twenty-one sample sets from maternal plasma, cord plasma and three stages of milk (colostrum: 1-5 days after delivery; transitional milk：10-15 days; mature milk: 40-45 days) were collected longitudinally in six regions of Shanghai, Guangzhou, Tianjin, Chengdu, Lanzhou and Changchun. α-Tocopherol and the stereoisomers were determined by high performance liquid chromatography with a fluorescence detector. In six regions of China, α-Tocopherol concentrations in maternal plasma and cord plasma were 15.38 mg/L, 2.12 mg/L and those of RRR were 13.17 mg/L and 1.82 mg/L respectively while those of synthetic α-Tocopherol were 2.21 mg/L and 0.30 mg/L respectively. As for the breast milk, they were 9.59 mg/L, 8.60 mg/L in colostrum; 3.68 mg/L, 3.23 mg/L in transitional milk; 3.01 mg/L, 2.47 mg/L in mature milk respectively. The RRR configuration accounted for more than 80% of α-Tocopherol in maternal plasma, cord plasma and breast milk. Overall, there were regional differences both in α-Tocopherol and RRR. There were significant correlations between α-Tocopherol and RRR in maternal plasma, cord plasma and milk (P=0.000). As well as negative correlations among α-Tocopherol, RRR and weight-related indicators, which refer to pre-gestation weight and BMI, pre-delivery weight and BMI and pregnancy weight gain, in almost all of samples. Although there were regional differences in RRR-α-Tocopherol concentrations, it was highly enriched both in maternal plasma and cord plasma. This study suggested that RRR-α-Tocopherol was the dominant configuration of α-Tocopherol and the main active form of vitamin E in the early life, guiding the rational supplement of pregnant women and the addition of α-Tocopherol in infant formula milk powder.

Clinical Observation of Extensively Hydrolysis Protein Formula With Feeding Intolerance in Preterm Infants.

ObjectiveTo investigate whether feeding extensively hydrolysis protein formula during the NICU hospitalization was more beneficial for preterm infants with a gestational age (GA) ≤34 weeks when breastfeeding was not possible.MethodsIn total, 587 preterm infants were randomly divided into two groups: observation groups fed with extensively hydrolyzed formula (EHF) milk and control groups fed with standard preterm formula (SPF) milk until discharge from the neonatal intensive care unit (NICU). The incidence of complications during hospitalization was recorded in both groups. Then, two groups were uniformly fed with 0-to-6-month infant formula milk and followed-up for 6 months after discharge.ResultsThe final study included 370 premature infants, including 185 babies in the observation group and 185 in the control group. In contrast to the SPF, feeding EHF among preterm infants of GA <34 weeks during NICU hospitalization significantly reduced the incidence of feeding intolerance (FI) (14.1 vs. 30.3%, p < 0.01). The incidence of necrotizing enterocolitis (NEC) was significantly reduced in the observation group (2.2 vs. 6.5%, p < 0.05), but there was no significant difference in the incidence of other related complications. At discharge, there was no difference in total serum protein (46.6 vs. 46.4 g/L), albumin (33.5 vs. 34.2 g/L), and calcium (2.37 vs. 2.35 mmol/L), but the serum phosphorus concentrations associated with skeletal mineralization (2.10 vs. 2.22 mmol/L, p < 0.05) was significantly reduced and alkaline phosphatase significantly rose (254 vs. 220 IU/L, p < 0.05) in the observation group. No significant difference was found in the growth rates of body weight, head circumference, or body length, either during the NICU hospitalization or during the 6-month follow-up after discharge (p > 0.05).ConclusionsFeeding premature infants of GA ≤34 weeks with EHF reduced the incidence of FI, but had no advantage in establishing whole intestinal nutrition, shortening parenteral nutrition (PN) time, or hospitalization time. It had little effect on physical growth or development during NICU hospitalization and within 6 months after discharge. However, it may increase the incidence of metabolic bone disease (MBD).

Surface free fat bridging contributes to the stickiness of powdered infant formula milk pasteurized by radio frequency dry heat treatment

The effects of radio frequency (RF) dry heat treatment on powdered infant formula milk (PIFM) stickiness were investigated. Commercial spray-dried PIFM with a water activity of 0.28 was treated at a constant temperature of 70 °C for 0, 23.3, 46.6, 69.9, 93.2, and 116.5 min, corresponding to 0–5 logarithmic inactivation of Cronobacter sakazakii . All RF treatments significantly reduced the water activity, while induced insignificant change in lactose crystallinity (∼2%–3%) and surface free fat content (∼0.005–0.006 g/g powder) compared with the untreated sample. The particle size of PIFM was increased markedly when the holding time reached 23.3 min, and after that changed insignificantly, suggesting that the stickiness between neighboring particles was completed at the initial stage of pasteurization. The stickiness of the powders was noted along with the flow of molten surface fat when processed for 23.3 min, but fat coverage decreased due to fat solidification as time increased from 23.3 min to 116.5 min. Compared with the untreated samples, the increased surface lactose coverage of treated PIFM reduced the critical water activity for glass transition from 0.39 to 0.31 at 25 °C. This study demonstrated that surface free fat bridges were responsible for increasing particle size after RF dry heat pasteurization. •Radio frequency treatment redistributed the surface free fat. •Surface fat bridge contributed to a significant increase in particle size. •Radio frequency distinctly increased the lactose exposure on the particle surface. • Radio frequency reduced the critical water activity for lactose glass transition.

Infants With Different Weight for Age Percentiles May Benefit From Stratified Daily Formula Milk Intake Recommendations

ObjectivesCurrently available guidelines on the daily formula milk requirements of infants, including those from the Institute of Medicine (IOM) and FAO/WHO/UNU, are based on the needs of infants with median body weight and the fact that their growth pattern follows the 50th percentile of the weight-for-age growth curve. Hence, current recommendations might not be sufficiently detailed to meet the needs of infants across the broad spectrum of body weight percentiles. This study aimed to provide stratified recommendations for daily formula milk intake of fully formula-fed infants across different weight-age categories from 0 to 4 months.MethodsAt first, theoretical age- and gender- specific weight ranges were constructed for infants across five predefined weight-for-length percentile categories of the WHO growth standard: small (10th percentile), medium-small (15th to 25th percentiles), medium (50th percentile), medium-large (75th to 85th percentiles), and large (90th percentile) infants. Thereafter, total daily energy requirements for each category were calculated using IOM equation and converted to daily formula milk needs. Subsequently, these stratified age- and weight- formula milk recommendations were compared to actual daily and relative formula milk of infants in these categories retrieved from pooled individual infant formula milk intake (6.174 data points, from 13 clinical intervention studies).ResultsQualitative comparison between theoretical calculation and actual formula milk intakes were both following the same trend with the median daily formula milk volume intake of infants increasing across increasing weight-for-age categories at each timepoint, with larger variations observed in “real life” data compared to theoretical recommendations. Interestingly, the relative daily formula milk volume intake (ml/kg/day) was relatively stable over time but was relatively higher in smaller infants compared to larger infants.ConclusionsQualitative evaluation of both theoretical calculations as well as real-life daily formula intake data showed that one fixed value as formula milk volume intake recommendation may not be optimal for the nutritional needs of infants growing at different percentiles of weight for age.Funding SourcesDanone Nutricia Research.

Minimal Effects of Medium-Chain Triglyceride Supplementation on the Intestinal Microbiome Composition of Premature Infants: A Single-Center Pilot Study.

Compared to term infants, the microbiota of preterm infants is less diverse and often enriched for potential pathogens (e.g., members of the family Enterobacteriaceae). Additionally, antibiotics are frequently given to preterm infants, further destabilizing the microbiota and increasing the risk of fungal infections. In a previous communication, our group showed that supplementation of the premature infant diet with medium-chain triglyceride (MCT) oil reduced the fungal burden of Candida spp. in the gastrointestinal tract. The objective of this study was to determine whether MCT supplementation impacts the bacterial component of the microbiome. Pre-term infants (n = 17) receiving enteral feedings of either infant formula (n = 12) or human milk (n = 5) were randomized to MCT supplementation (n = 9) or no supplementation (n = 8). Fecal samples were taken at randomization and prior to MCT supplementation (Week 0), on days 5–7 (Week 1) and day 21 (Week 3). After DNA extraction from samples, the QIIME2 pipeline was utilized to measure community diversity and composition (genera and phyla). Our findings show that MCT supplementation did not significantly alter microbiota diversity or composition in the gastrointestinal tract. Importantly, there were no significant changes in the family Enterobacteriaceae, suggesting that MCT supplementation did not enrich for potential pathogens. MCT holds promise as a therapeutic intervention for reducing fungal colonization without significant impact on the bacterial composition of the host gastrointestinal tract.

Comparative Analysis of Protein in Selected Infant Formula Using Dye-Binding and Formol Methods

Aim: The protein content of some infant milk formula was determined using Dye-binding and Formol methods in comparison to the manufacturer’s values as reference.&#x0D; Study Design: The study was an experimental design.&#x0D; Place and Duration of Study: This study was carried out at the laboratory of Chemistry department, Federal University of Agriculture, Makurdi, Nigeria between June, 2021 and December, 2021.&#x0D; Methodology: Dye-binding Method was done by weighing about 5 g of the baby milk samples into beakers and 100 mL distilled water was added and mixed thoroughly. 20 mL of diluted 80% orange G dye solution was added to 2 mL of the milk solution and shaked vigorously. The sample was centrifuged at 820 rpm for 5 minutes and a clear filtrate obtained whose absorbance was then analyzed using spectrophotometer. For Formol Method I g of each of the milk samples was weighed into different beakers then 10 mL of distilled water and 0.7 mL saturated potassium oxalate was added along with 3 drops of phenolphthalein indicator into the mixture. Two drops of NaOH solution was titrated into the mixture and a pink colour appeared which diminished immediately. About 2 mL of formaldehyde was added and shaken for few seconds then titrated using sodium hydroxide (NaOH) until the mixture turned light pink.&#x0D; Results: The mean and standard deviation of protein content using Dye-binding method are; Peak baby milk 2.49 ± 0.13 g, My boy milk 2.54 ± 0.06 g. For formol methods Peak baby milk has 0.13 ± 0.01 g and My boy milk 0.1 ± 0.01 g. The result showed that both infant formula under the Dye-binding method and formol method was significantly different (p&lt;0.05) when compared to the reference value (1.7 Peak baby and 0.11 My boy). However, values obtained from Formol method for My boy milk was closest to the reference (factory value) only a slight difference of about 0.01 g.&#x0D; Conclusion: In conclusion, Formol method is more closely related to the reference values (manufacturer’s values). Thus, the Formol titration may be used with confidence as a quick test for approximating the protein content of skim milk solids which is typical of baby milks.

Prevalence of Bacillus cereus in dairy powders focusing on its toxigenic genes and antimicrobial resistance

Bacillus cereus is a common environmental foodborne microorganism that is mainly found to harbor toxigenic genes with multiple antibiotic resistances and is linked to threatening the safety of dried milk in concern to powdered infant milk formula. In the current investigation, the mean value of B. cereus in 140 samples of powdered milk was 0.57 × 102 ± 0.182 × 102, 0.15 × 102 ± 0.027 × 102, 0.21 × 102 ± 0.035 × 102, and 0.32 × 102 ± 0.072 × 102 CFU/g in a percentage of 64.0 samples of whole milk powder, 43.3 of skim milk powder, 26.7 of powdered infant milk formula and 36.7 milk–cereal-based infant formula, respectively. The results revealed that B. cereus isolates were found to harbor toxigenic genes in the following percentages: 77.8, 2.0, 72.7, 16.2, and 67.7 for nhe, hbl, cytK, ces, and bceT, respectively. Despite all evaluated B. cereus strains were originated from dairy powders, they showed a significant difference (P < 0.05) in their harbored toxigenic cytK gene between whole and skim milk powders with powdered infant formula and milk–cereal-based infant formula, as well as between powdered infant formula and milk–cereal-based infant formula. All isolated B. cereus strains were resistant to cefoxitin, colistin sulfate, neomycin, trimethoprim–sulfamethoxazole, oxacillin, and penicillin. Based on the antimicrobial resistance of B. cereus strains to cephalothin, chloramphenicol, nalidixic acid, and tetracycline, there was a significant difference (P < 0.05) between powdered infant milk formula and whole milk powder strains. This survey is one of few studies proceeded in Egypt to determine the prevalence of toxigenic B. cereus strains in milk–cereal-based infant formula and powdered infant formula as well as skim milk powder.

Quantitative Determination of Whey Protein to Casein Ratio in Infant Formula Milk Powder.

This study was aimed to establish a method for quantitatively determining the ratio of whey protein in the total protein of infant formula by respectively selecting two characteristic peptides from whey protein and casein and calculating the ratio between the characteristic peptides. A nanoliter high-performance liquid chromatography tandem high-resolution mass spectrometry (Q Exactive) was used to simultaneously detect the characteristic peptides of two main whey proteins and two main caseins. The characteristic peptides were calculated, predicted, and screened using the ExPASy website, and peptide information was confirmed by database retrieval after the analysis by using a high-resolution mass spectrometer. The matrix effect was compensated by comparing the characteristic peptides in whey protein with those in casein protein, in which isotope internal standards were not required. The influence of the changes of the protein content in whey protein and casein on the detection method was eliminated by the calculation formula designed by ourselves. In this detection method, the sample was stable in the total protein concentration range of between 0.1 and 0.4 mg/ml. In the simulated industrial processing environment, with desalted whey powder, the recovery rate was 98.63–113.33% under different spiked levels with good reproducibility (RSD<8%). The RSDs of intraday and interday precisions were 2.03–9.35% and 0.61–11.02%, respectively. The different processing procedures of samples had no significant impact on the detection of whey protein (RSD% for milk samples treated by different processing techniques was 2.97%). The quantitation method of whey protein was applied to evaluate the whey protein content in different brands of commercially available milk powder. In summary, the proposed method was applicable for quantitative analysis of whey proteins in the infant formula.

Breakage behaviour and functionality of spray-dried agglomerated model infant milk formula: Effect of proteins and carbohydrates content

This study explored the effect of protein content (whey protein and casein) and carbohydrate content (lactose, sucrose, and maltodextrin) on the breakage behaviour and its influence on spray-dried agglomerated model infant milk formula. Whey protein powders were bigger in particle size, weaker in structural strength, and marginally more irregular in shape, which resulted in better rehydration properties but more breakage than pure casein powders. Similarly, sucrose samples had better rehydration properties and higher glass transition temperatures but suffered more breakage than maltodextrin and pure lactose powders because of their bigger particle size. The influence of proteins on breakage was greater than that of carbohydrates. Breakage changed the physical and structural properties of powders, especially for whey protein and sucrose samples, which caused the deterioration of rehydration properties and the decrease in crystallization temperatures. From the perspective of particle breakage, unwanted dairy powder breakage could be controlled by changing powder formulations.

Nutritional and Safety Concerns of Infant Feeding Trends.

The American Academy of Pediatrics (AAP) and North American Society for Pediatric Gastroenterology, Hepatology and Nutrition recommend either exclusively breastfeeding for at least 6 months or an u.S. Food and Drug Administration-reviewed infant formula or donor breast milk from an established milk bank as alternatives. The purpose of this study was to establish the prevalence of contemporary infant feeding practices such as informal human milk sharing, imported European infant formula, toddler formula and homemade formula and gain insight into the parental reasoning for their choices. An anonymous, cross-sectional, voluntary electronic survey was sent to active prescribers to a Yumi (a baby food subscription company) list server in April and May 2021. Basic demographic, utilization of infant feeding practices and general feeding practices were collected. Of 2315 respondents, at least 18% of the families were following at least one contemporary feeding practice. Thirty six percent of parents using donor breast milk obtained it from unregulated sources, 14% of the respondents were using European infant formula, 5% were using toddler formula for their infants, and 2% were making homemade infant formula. The AAP has clear guidelines on infant nutrition and breastfeeding and when not possible, FDA reviewed infant formula or donor breast milk from an established milk bank as alternatives. Yet, our study found that at least 18% of the families across the united States were following at least one contemporary feeding practice with possible nutritional and safety concerns. it is important for pediatric gastroenterologists and dieticians to ask their patients how they are feeding their infants and be aware of these feeding practices that may pose significant health risks.

Effects of different breakage mechanisms on the physical, water sorption, and mechanical characteristics of infant milk formula

Two main breakage mechanisms, surface breakage and body breakage, were both found during laboratory dilute-phase pneumatic conveying and high-speed mixing of infant milk formula, respectively. Body breakage had a greater influence on powder physical properties, particle shapes, and surface compositions than surface breakage. In particular, the particle size of samples decreased by only 10% after surface breakage but by 60% after body breakage. For samples where body breakage predominated, significant changes to both water sorption and mechanical properties of the powder were seen while surface breakage had very little effect on them. Body breakage significantly increased the water sorption rate of samples, at around 10%–126% higher than the other samples for the first 3 h, which accelerated the time-dependent crystallization. Moreover, at a W 0.11 to 0.33, the final water contents of body breakage samples were 3.5%–22% higher than control samples. This decreased the crystallization temperature, by between 2 °C and 10 °C, and the overall molecular mobility of samples. These changes were due to the increase in the number of adsorbed monolayers, sorption surface area, and monolayer value of samples after body breakage. • Surface breakage affected physical properties but not water sorption behavior. • Body breakage greatly deteriorated physical and surface properties. • Body breakage increased water sorption rates, monolayer values and water contents. • Body breakage decreased the instant crystallization and overall molecular mobility. • Body breakage slightly decreased glass transition and α-relaxation temperatures.

Early Life Exposure to a Diet With a Supramolecular Lipid Structure Close to That of Mammalian Milk Improves Early Life Growth, Skeletal Development, and Later Life Neurocognitive Function in Individually and Socially Housed Male C57BL/6J Mice.

Breastfeeding (duration) can be positively associated with infant growth outcomes as well as improved cognitive functions during childhood and later life stages. (Prolonged) exposure to optimal lipid quantity and quality, i.e., the supramolecular structure of lipids, in mammalian milk, may contribute to these beneficial effects through nutritional early-life programming. In this pre-clinical study, we exposed male C57BL/6J mice from post-natal Days 16 to 42 (i.e., directly following normal lactation), to a diet with large lipid droplets coated with bovine milk fat globule membrane-derived phospholipids, which mimic more closely the supramolecular structure of lipid droplets in mammalian milk. We investigated whether exposure to this diet could affect growth and brain development-related parameters. As these outcomes are also known to be affected by the post-weaning social environment in mice, we included both individually housed and pair-wise housed animals and studied whether effects of diet were modulated by the social environment. After Day 42, all the animals were fed standard semi-synthetic rodent diet. Growth and body composition were assessed, and the mice were subjected to various behavioral tests. Individual housing attenuated adolescent growth, reduced femur length, and increased body fat mass. Adult social interest was increased due to individual housing, while cognitive and behavioral alterations as a result of different housing conditions were modest. The diet increased adolescent growth and femur length, increased lean body mass, reduced adolescent anxiety, and improved adult cognitive performance. These effects of diet exposure were comparable between individually and socially housed mice. Hence, early life exposure to a diet with lipid droplets that mimic the supramolecular structure of those in mammalian milk may improve adolescent growth and alters brain function in both socially and individually housed mice. These findings suggest that lipid structure in infant milk formula may be a relevant target for nutritional solutions, targeting both healthy infants and infants facing growth challenges.

Detection and Quantification of Immunoregulatory miRNAs in Human Milk and Infant Milk Formula.

Mammary gland secretory cells produce miRNA-rich milk. In humans, these miRNAs reach infant/neonate bloodstream, playing diverse roles, like neural system development, metabolism, and immune system maturation. Notwithstanding, still few works explore human milk miRNA content, and there are no reports at the population level. Our hypothesis was that miR-146b-5p, miR148a-3p, miR155-5p, mir181a-5p, and mir200a-3p immunoregulatory miRNAs are expressed in human colostrum/milk at a higher level than infant milk formulae. The aim of this work was to evaluate the expression of the five immunoregulatory miRNAs in human milk and compare it with their expression in infant milk formula. For this purpose, miRNA relative expression was measured by qPCR in cDNA prepared from total RNA extracted from sixty human colostrum/milk samples and six different formulae. The comparative Cт method 2−ΔCт using exogenous cel-miR-39 as internal control was employed, followed by statistical analysis. We found the relative expression levels of miRNAs are comparable among colostrum/milk samples, and these miRNAs are present in infant milk formulae but at very low concentrations. We conclude that the relative expression of the immunomodulatory miRNAs is comparable in all the human colostrum/milk samples and is higher than the expression in formulae.

Antibiotic Resistance and Molecular Characterization of Cronobacter sakazakii Strains Isolated from Powdered Infant Formula Milk.

Background: Cronobacter sakazakii is a new emerging foodborne bacterial pathogen associated with severe lethal diseases such as meningitis, necrotizing enterocolitis, and septicemia in infants and neonates. Powdered infant formula milk (PIFM) has been recognized as one of the main transmission vehicles and contaminated sources of this pathogen. This study aimed to investigate the prevalence rate, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from 364 PIFM samples collected from Tehran city, Iran. Methods: Culture-based methods, Kirby–Bauer disk diffusion antibiotic resistance testing, conventional Polymerase Chain Reaction (PCR), and Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) assays were used in this study to detect and characterize the C. sakazakii isolates. Results: We isolated 25 C. sakazakii strains from PIFM samples (6.86%). The isolates were highly resistant to amoxicillin-clavulanic acid, amoxicillin, ampicillin, cefoxitin, cefepime, erythromycin, ceftriaxone, ciprofloxacin, and chloramphenicol and susceptible to gentamicin, tetracycline, norfloxacin, and azithromycin antibiotics. The blaCTX-M-1 gene was detected in 96% of the isolates. The isolates were categorized into eight distinct clonal types using the ERIC-PCR method, showing a high genetic diversity among the isolates. However, there was a significant correlation between the genotypic and phenotypic antibiotic resistance properties of the isolates. Conclusions: Novel microbial surveillance systems for detecting multi-drug-resistant C. sakazakii are required to control the contamination of this foodborne pathogen in infant foods.

Alkyl chain length modulates antioxidant activity of gallic acid esters in spray-dried emulsions

Lipid oxidation is a well-recognized issue in dried food emulsions, such as infant milk formula. Antioxidants can be used to mitigate this issue; however, their efficiency in such complex systems is far from understood. In this study, antioxidant polarity is varied through the alkyl chain length of gallic acid esters (0–16 carbon atoms) incorporated to O/W emulsions that are subsequently spray-dried. During processing and subsequent storage of the samples, antioxidants with more than eight carbon atoms are effective. Both for encapsulated fat and surface free fat, we observe a slight cut-off effect, meaning that beyond eight alkyl groups, a more nonpolar antioxidant is slightly less effective. Depending on the antioxidant polarity, lipid oxidation is faster either in the encapsulated or in the surface free fat. The insights obtained contribute to understanding lipid oxidation in low moisture food emulsions, and thus lead to effective antioxidant strategies.

A worldwide systematic literature review for aflatoxin M1 in infant formula milk: Human health risk assessment by Monte Carlo simulation

Mycotoxins, predominantly aflatoxin M1 (AFM1), require better quality control in infant formula milk (IFM). This study aims to use a systematic review study to estimate the amount and health risk assessment of AFM1 of IFM for infants less than one year old in the last two decades worldwide. For this purpose, a systematic search was conducted from June 27, 2000, to April 5, 2021. Two hundred and twelve articles and reports were obtained from various databases, and twenty of them were eventually chosen for the current study based on the study's selection criteria. Due to a lack of access to the raw data of the studies obtained, EXCEL re-simulated the data of each of these studies, keeping in mind the mean, standard deviation, and sample size of each study. The statistical analysis, health risk assessment, uncertainty analysis, and sensitivity analysis for AFM1 of IFM were then done on the simulated data by country, continent, and globally. Based on the compliance of AFM1 of IFM with the European Community (EC) standard (25 ng/kg), and also according to the results of health risk assessment, the quality of IFM provided to the market in most countries in the last two decades in terms of the AFM were at a high level and has been unauthorized. Therefore, it is necessary to pay special attention to continuous monitoring and control measures for AFM1 in consumer IFM over all countries worldwide. Due to the limited current information about AFM1 of IFM in the world, further studies are necessary.

Determination of Benzo(α)pyrene in Infant Formula Using High Performance Liquid Chromatography and Dispersive Liquid–Liquid Microextraction

Background: Benzo(α)pyrene (BaP) is one of the most familiar polycyclic aromatic hydrocarbons (PAHs) to evaluate food safety and quality and can be present in infant formulas An analytical method for the extraction and quantification of BaP in infant formula milk has been established by dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detector. Methods: BaP was first extracted from matrices of infant formula milk via acetonitrile and then DLLME was used for further purification and preconcentration of target analyte. Results: Under the optimum extraction conditions, 150 µL of dichloromethane as extraction solvent, 3 mL acetonitrile as disperser and cleaner solvent and 1 mL volume sample, the accuracy of the method was between 89-97%. The limits of detection and quantification were 0.12 and 0.35 ng/ml, respectively. There was a linear relation (R2=0.998) between chromatographic peak area and concentrations in the range of 0.5 to 15 ng/mL. Conclusion: The proposed method is applicable to the quantification of BaP in infant formula milk with acceptable accuracy and precision.

An extremely sensitive carbon paste electrode modified with Prussian blue analogue (PbA @CPE) for the electrochemical determination of Tetramisole HCl anthelmintic drug as a food contaminant in beef cuts and infant formula milk powder

Tetramisole hydrochloride (TMS) - the drug of choice for ascariasis- is used as an anthelmintic. In this paper, both qualitative and quantitative determination of TMS in bulk, infant milk powder, and frozen cuts-up bovine meat samples were developed to provide consumersafety. In this paper, chemically modified carbon paste with Prussian blue analogue nanoparticles (PbA-NPs /CP) was developed for determination of TMS utilizing cylic voltammetry (CV), square wave voltammetry (SWV) and chronoamperometry methods. In this regard, Prussian blue analogue (PbA) was synthesized by the chemical co-precipitation route. XRD technique was used for crystal structure analysis indicating that the fabricated nanopowder belongs to a cubic structure with lattice constants a=b=c=10.176AThe grain size was estimated approximately equal 20 nm, and the dislocation density was 25×10-4nm-2. The molecular structure was analyzed using Raman spectroscopy, and the elements C, N, O, Co, Ni, Cr, Ir were recorded by EDX spectrum. SEM images showed the particles very fine in spherical shape. Transmittance and reflectance spectra have been measured through wavelength range from 200 nm to 900 nm.Energy gap (Eg) and refractive index (n) of the films were determined suggesting the strong optical absorption. Finally, this method was carried out at pH 4.0 within a linearity range (10.0 × 10−9–1.0 × 10−4) M and a minimum detection limit of 2.7 × 10−9M.

Metalloporphyrin and gold nanoparticles modified hollow zeolite imidazole Framework-8 with excellent peroxidase like activity for quick colorimetric determination of choline in infant formula milk powder

Metalloporphyrin and gold nanoparticles (AuNPs) were fixed on the surface of hollow zeolite imidazole framework-8 (HZIF-8) by cation exchange and cross-linking reaction. The obtained hybrid nanozyme, Au/HZIF-8@TCPP(Fe), was fully characterized by TEM, HRTEM, EDS element mapping and XPS. Then, its peroxidase-like activity was explored with Km of 1.74 mM and Vmax of 9.60 × 10-8 M·S-1 towards H2O2, indicating excellent catalytic activity. Based on cascade reaction between choline oxidase and Au/HZIF-8@TCPP(Fe), a quick colorimetric method was established for choline detection in infant formula milk powder. After comprehensive verification, this method presented the merits of simple operation, satisfied detection limit (0.05 mM), wide linear range (0.05–2.0 mM), high accuracy (recovery of 92.2%-105.2%) and nice selectivity. This colorimetric method was applied to the determination of choline in milk powders of five brands. Our study could offer valuable reference for finding highly efficient nanozyme and constructing novel optical biosensors.