Infant Human Immunodeficiency Virus Research Articles

Laboratory Diagnosis of HIV Infection

Laboratory diagnosis of human immunodeficiency virus (HIV) infection is complicated by absence of data on sensitivity, specificity and predictive value of the various tests as they apply to children. The presence of maternal anti-HIV passively transmitted across the placenta also confounds diagnosis. The authors review currently available data on the detection of HIV, HIV genome, and HIV gene products, as well as the diagnostic value of detecting serologic and cellular responses to HIV in infants and children.

Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers

International Journal of Gynecology & ObstetricsVolume 31, Issue 1 p. 99-99 Citations from the literature oncology Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers MF Rogers, MF RogersSearch for more papers by this authorC-Y Ou, C-Y OuSearch for more papers by this authorM Rayfield, M RayfieldSearch for more papers by this authorPA Thomas, PA ThomasSearch for more papers by this authorEE Schoenbaum, EE SchoenbaumSearch for more papers by this authorE Abrams, E AbramsSearch for more papers by this authorK Krasinski, K KrasinskiSearch for more papers by this authorPA Selwyn, PA SelwynSearch for more papers by this authorJ Moore, J MooreSearch for more papers by this authorA Kaul, A KaulSearch for more papers by this authorKT Grimm, KT GrimmSearch for more papers by this authorM Bamji, M BamjiSearch for more papers by this authorG Schochetman, G SchochetmanSearch for more papers by this author MF Rogers, MF RogersSearch for more papers by this authorC-Y Ou, C-Y OuSearch for more papers by this authorM Rayfield, M RayfieldSearch for more papers by this authorPA Thomas, PA ThomasSearch for more papers by this authorEE Schoenbaum, EE SchoenbaumSearch for more papers by this authorE Abrams, E AbramsSearch for more papers by this authorK Krasinski, K KrasinskiSearch for more papers by this authorPA Selwyn, PA SelwynSearch for more papers by this authorJ Moore, J MooreSearch for more papers by this authorA Kaul, A KaulSearch for more papers by this authorKT Grimm, KT GrimmSearch for more papers by this authorM Bamji, M BamjiSearch for more papers by this authorG Schochetman, G SchochetmanSearch for more papers by this author First published: January 1990 https://doi.org/10.1016/0020-7292(90)90234-CAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume31, Issue1January 1990Pages 99-99 RelatedInformation

Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers. New York City Collaborative Study of Maternal HIV Transmission and Montefiore Medical Center HIV Perinatal Transmission Study Group.

The early diagnosis of infection with human immunodeficiency virus (HIV) in infants born to infected mothers is essential for early treatment, but current tests cannot detect HIV infection in newborns because of the presence of maternal antibodies. We used the polymerase chain reaction, a new technique that amplifies proviral sequences of HIV within DNA, to detect HIV infection in peripheral-blood mononuclear cells obtained from infants of seropositive women during the neonatal (age less than 28 days) and postneonatal periods. In blood obtained during the neonatal period, the polymerase chain reaction was positive in five of seven infants in whom the acquired immunodeficiency syndrome (AIDS) later developed (a mean of 9.8 months after the test). The test was also positive in one of eight newborns who later had nonspecific signs and symptoms suggestive of HIV infection (mean follow-up, 12 months). No proviral sequences were detected in neonatal samples from nine infants who remained well (mean follow-up, 16 months). HIV proviral sequences were detected in samples obtained during the postneonatal period (median age, five months) in all of 6 infants tested who later had AIDS and in 4 of 14 infants with nonspecific findings suggestive of HIV infection. No proviral sequences were detected in 25 infants who remained well (mean follow-up, 17 months) after being born to HIV-seropositive mothers, or in 15 infants born to HIV-seronegative mothers. We conclude that the polymerase chain reaction will be a useful technique to diagnose HIV infection in newborns and to predict the subsequent development of AIDS. However, larger studies will be required to determine the sensitivity and specificity of the test.