Results: Differences between rigid body measuring device, the MoCap laboratory and the cross table were not severe enough to limit the gait analysis capabilities of the system. The differences between inexperienced and experienced examiners were present. The application of themarker placement protocol showed differences between the examiners, however once the marker trajectories are translated into joint kinematics the differences were non-significant. Discussion and conclusions: This study introduces a method to measure the accuracy and sensitivity of a fully equipped motion capture gait laboratory, a new marker placement protocol and the examiners conducting the gait analysis. The results indicate differences between examiners. However they mostly disappeared after a learning curve of the persons. Also they had only little influence on the kinematics derived from the marker trajectories. This article analyses a new marker protocol and gait lab set up on several levels and thus validates the accuracy and sensitivity of the system which is crucial in order to be able to make valid statements about gait kinematics.