Abstract Dual-axis rotational inertial navigation system (RINS) can effectively suppress the error accumulation of inertial navigation system, thereby improve the long-term navigation accuracy through rotating inertial measurement unit (IMU) according to corresponding rotation scheme. The navigation accuracy of RINS is predominantly contingent on the IMU rotation scheme. A reasonable rotation scheme can effectively attenuate or even obviate the navigation errors stemming from IMU errors, whereas an ill-considered scheme may lead to swift divergence of navigation errors. In this paper, the principle of IMU errors suppression is meticulously analyzed during rotation. By comprehensively evaluating the impact of individual IMU errors on navigation accuracy alongside their corresponding error modulation characteristics, a novel 32-position rotation scheme is devised. The proposed scheme demonstrates a notable capacity to minimize the adverse effects of IMU errors on navigation accuracy and to enhance error suppression efficacy within a modulation cycle. Meanwhile, the long-endurance navigation accuracy of RINS is substantially enhanced. The efficacy of the 32-position scheme posited herein is empirically substantiated through a 24 h simulation and navigation experiment.