Hermetia illucens larvae (HiL) has been studied because of its capability to break down organic waste. HiL can convert waste biomass efficiently, that can be used as future feedstuffs. This study aimed to evaluate the fatty acids composition from HiL oil (HiLO) which reared on the different substrates. The experimental was compared of (1) HiL reared on palm kernel meal and (2) HiL reared on 80% industrial waste mix with 20% organic waste. The chemical composition of HiL measured base on the Association of Official Analytical Chemists (AOAC). The Hermetia illucens larvae oil (HiLO) was extracted according to the modification of the Soxhlet extraction procedure. Fatty acid compositions were analyzed using gas chromatography (GC). The data collected and analyzed as descriptive. The results of this study ether extract content of HiL (1) 51.46% higher than HiL (2), this is due to substrate types. Fatty acids of both are almost the same and the most dominant are lauric acid 40.54% and 46.72%, oleic acid 17.48% and 15.98%, palmitic acid 14.55% and 12.12% and myristic acid 15.57 and 11.13 %, respectively for each HiLO (1) and (2). The conclusion of this study is the HiL reared on different substrates affect lipid content but the fatty acid composition is similar.
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