Industrial Organic Wastes Research Articles

Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains

This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main Δ9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.

Effect of Sewage Sludge and Rice Straw Compost on Yield, Micronutrient Availability and Soil Quality under Rice–Wheat System

ABSTRACTThere is a need to improve the way in which crop residues and industrial organic wastes are managed and also to study their potential use in agriculture for improving soil fertility and biological activity. This study evaluated the effects of integrated use of organic (sewage sludge (SS) and rice straw compost (RSC)) and inorganic fertilizers on crop yield, soil enzymes activity, macro- and micro-nutrients availability under rice–wheat cropping system after three consecutive years of cropping in a subtropical semi-arid area. Different combinations of inorganic nitrogen and two doses of organic sources (SS and RSC) were applied to the soil. The results revealed that substitution with 50% N through RSC significantly increased the yield and biochemical properties as compared to inorganic fertilizers (NPK) alone. Micronutrients availability was found increased in treatment having substitution of 50% N through SS @10 t ha−1. All the enzymatic activities viz. dehydrogenase, fluorescein diacetate (FDA), phosphatase, phytase, and urease) were found to be maximum by substitution of 50% N through RSC. Also, a significant positive correlation was found between soil enzymes (dehydrogenase and FDA) and organic carbon as well as crop yield. Thus, the study demonstrated that substitution of 50% inorganic nitrogen through organic sources will be a better alternative for improving soil quality and productivity.

The biogas plant in Kalmar: possibilities and experiences

The use of biological anaerobic treatment for industrial organic waste to close nutrient circulation in society. The organic waste has an clearly potential for reducing use of the earth's resources and will be of essential importance for human living and foodproduktion in an near future. The paper will give an example of one way to reuse of nutrients back to the farmland, and how earlier Agenda 21 work leads to a better society.

Studi adsorpsi limbah organik industri tahu tempe dengan karbon aktif kayu merbau [Intsia bijuga (Colebr) O. Kuntze

Study on adsorption of tofu and tempe industrial organic waste with timber wood [Intsia bijuga (Colebr) O. Kuntze] as the active carbon has been executed. The objective the research is to determine the optimum required consentration of HCl to active the coal of timber wood, discover optimum adsorption capacity of timber charcoal to reduce the level of BOD5 dan COD in fluids waste of tofu and tempe industry. Research method of activating timber wood charcoal is physically by heating 700oC of temperature and chemically by submersion in HCl 1M, 2M and 3M. First, variation is made of contact duration of timber wood active carbon and fluid waste for 30, 60, 90 and 120 minutes. Then, volume of fluid waste is also differed by 100, 150, 200, 250 and 300 ml per 0.5 gram of active carbon. Result of the research shows that optimum condition for adsorption of tofu and tempe industrial fluid waste are as follow : at consentrate of HCl 3M, contact duration 30 minutes, fluid waste volume 250 ml per 0.5 gram of active carbon, optimum adsorption capacity of timber wood active carbon is achieved which lowering the level of BOD5 and COD of Rizky tofu and tempe industrial fluid waste; each by 60,600 mg/g and 12,500 mg/g. On the state where volume of the waste is 100 ml per 0.5 gram, optimum adsorption capacity of the timber wood active carbon in reducing the BOD5 and COD level of Sukamaju tofu and tempe industrial fluid waste is 82,400 mg/g and 164,200 mg/g each.

CURRENT UNDERSTANDING ON INDUSTRIAL ORGANIC WASTE MANAGEMENT BY EMPLOYING Eisenia fetida

Earthworms have the inherent capability of stabilising different organic waste materials of industrial as well as domestic origin. This review paper depicts the potential of epigeic earthworm species Eisenia fetida in the vermistabilisation of various industrial organic wastes such as silk industry waste, herbal pharmaceutical industry sludge, food industry sludge, milk processing industry sludge, tea factory coal ash, sugar mill sludge, cotton industrial waste, paper mill waste, and bio sludge of beverage industry. The analysis of results obtained clearly indicates the suitability of vermicomposting over conventional composting as an alternative technology for management, stabilisation and nutrient recovery from industrial organic waste.

Electrochemical sensing of free radical antioxidant diphenylamine cations (DPAH˙+) with carbon interlaced nanoflake-assembled MgxNi9−xS8 microspheres

The detection and control of free radical antioxidant diphenylamine cations (DPAH˙+), a typical organic industrial waste water byproduct, is important for environmental safety and protection.

Quantification of anaerobic digestion feedstocks for a regional bioeconomy

Anaerobic digestion (AD) for biogas production forms one of the fundamental building blocks of the bioeconomy, and a research programme has been under way in Northern Ireland, which culminated in the publication of a Biogas Research Action Plan 2020 in 2014. One important element of this programme was the identification of the need for an evidence base for the potential bioresource feedstocks. This paper reports the outputs of the quantification of feedstocks for AD research, which has identified the organic feedstocks available for biogas production on a regional basis and categorised these as: organic (biodegradable) fraction of municipal solid waste, sewage sludge, organic industrial and commercial wastes, manure from livestock, food wastes and energy crops. The research study further quantified the biogas and energy potential of these feedstocks and possible reductions in greenhouse gas emissions. The limitations of the research study are acknowledged and opportunities to address these and build on and extend the study are identified, including feedstocks for other bioeconomy processes and the application and further development of the biorefinery concept.

Biotransformation of Ternary Mixture of Organic Industrial Waste into Poultry Feed

Biotransformation of Ternary Mixture of Organic Industrial Waste into Poultry Feed

A Comprehensive Review of the Fate of Pathogens during Vermicomposting of Organic Wastes

Management of both municipal and industrial organic wastes remains a major threat to biota and the environment due to the presence of pathogens in abundance. Vermicomposting employing earthworms is increasingly gaining attention as a sustainable and ecofriendly technique to transform and sanitize a variety of organic wastes into nutrient-rich biofertilizer. Although considerable research has been undertaken to show that vermicomposting can significantly reduce pathogenic contents, there is little effort to summarize the various mechanisms responsible for it. With the aim to assess the fate of pathogens during vermicomposting of various organic wastes, this article provides a comprehensive summary on the occurrence of pathogens in a variety of wastes vis-à-vis pathogens standards, the efficacy of the process for pathogen reduction, and current knowledge of the plausible mechanisms involved. It is evident from the present study that earthworms and endosymbiotic microbes during vermicomposting tend to eliminate pathogens by enhancing enzymatic activities in both gut- and cast-associated processes. Pathogen reduction during vermicomposting can be plausibly attributed to direct actions like microbial inhibition due to intestinal enzymatic action, and secretion of coelomic fluids with antibacterial properties, as well as indirect actions like stimulation of endemic microbes leading to competition and antagonism, and aeration by burrowing activity. Further, the pathogen reduction during vermicomposting is largely selective, and earthworms exert a differential effect according to the earthworm species and whether the pathogen considered is Gram-positive or -negative, owing to its cell wall composition. However, further research is necessary to understand the exact mechanisms involved for pathogen reduction during vermistabilization of municipal and industrial organic wastes.

Improvement of Soil Properties, Growth of Cucumber and Protection against Fusarium Wilt by Piriformospora indica and Two Industrial Organic Wastes

The current work was focused on characterizing bagasse ash (BA) and press mud (PM) as soil amendments and to study their effect in combination with the endophytic fungus Piriformospora indica on Fusarium wilt (FW) of cucumber caused by Fusarium oxysporum f. sp. cucumerinum (Fo). Whereas BA and PM improved almost all physico-chemical properties of the soil evaluated, seed treatment with P. indica had no such effect. In shake culture in potato dextrose broth (PDB) medium amended with aqueous extracts of BA and PM, alone or in combination, production mycelial mass of Fo was significantly decreased by PM extract, while production mycelial mass of P. indica was highly improved. The colonization rate of cucumber roots by P. indica as determined by microscopy was highly increased by increasing amounts of BA, PM and BA+PM added to the soil. Seed treatment of cucumber with P. indica before plant cultivation in non-amended soil significantly decreased the disease severity of FW and improved plant growth. When seed treated with P. indica was sown into soil amended with BA, PM or the combination of both, the disease severity was even more reduced than after seed treatment with P. indica alone. In this respect, amendment with PM was more effective than with BA, and the combinations were more effective than the single treatments. Hence, there is a scope to integrate PM and BA as soil amendments in combination with P. indica for eco-friendly FW management, improving soil properties and growth of cucumber plants.

In-Situ Stability Control of Energy-Producing Anaerobic Biological Reactors through Novel Use of Ion Exchange Fibers

Anaerobic biological treatment of high-strength organic industrial wastes is preferred over aerobic treatment as it produces a methane-rich biogas, has much lower energy requirements, and produces significantly less biosolids. Process stability and reactor failure are of concern, however, for waste streams that exhibit large variations in organic loading, which can cause detrimental pH fluctuations, and that have the potential for accidental input of toxic metals. Here, we demonstrate for the first time that the use of ion exchange fibers (IXFs) can provide passive resilience to these failure modes, without requiring operator oversight or reactive process control via chemical addition. IXFs have the advantage of rapid kinetics due to their small size, and they can be readily inserted and withdrawn as woven mats or porous pillows. This approach is demonstrated here using the weak-acid IXF FIBAN X-1 and the strong-base FIBAN A-1. FIBAN X-1 passively stabilized anaerobic reactors by (i) buffering pH fluctuat...

Utilization of fruit processing industry waste as green activated carbon for the treatment of heavy metals and chlorophenols contaminated water

Plum stones, as a part of industrial and municipal organic waste, were used as a precursor for preparation of a low-cost activated carbon. Engineered, thermochemically-modified adsorbent was used to remove lead (Pb2+), cadmium (Cd2+), nickel (Ni2+) and chlorophenols from an aqueous solution. The characterization of the medium was performed using standard instrumental analysis. Additionally, the assessment included the influence of pH, adsorbent dosage, temperature, contact time and initial metal concentration on the separation efficiency in the batch-operational mode. With optimal working conditions, the process efficiency of over 95% was accomplished. The equilibrium and kinetic studies of adsorption were done. The pseudo-second order model described the adsorption kinetics best. The maximum adsorption capacity of the engineered adsorbent for Pb2+, Cd2+ and Ni2+ ions was calculated from the Langmuir isotherms and found to be 172.43 mg g−1, 112.74 mg g−1 and 63.74 mg g−1, respectively. Preliminary results indicate a strong affinity of the separation medium for chlorophenols. Thermodynamic parameters such as Gibbs energy, enthalpy and entropy were calculated. Regeneration of the saturated adsorbent was conducted, with diluted phosphoric acid produced as a waste stream, during the washing of the adsorbent after activation. Based on the desorption study results, the activated carbon was successfully regenerated in 3 cycles. Mutual influence of ions was analyzed in multicomponent systems. The real system production and operational costs analysis confirmed a possibility for a successful implementation of the highly efficient, eco-friendly engineered adsorbent in the field of cost-effective wastewater treatment.

The influence of decreased hydraulic retention time on the performance and stability of co-digestion of sewage sludge with grease trap sludge and organic fraction of municipal waste

The effect of hydraulic retention time ranging from 12 to 20 d on process performance and stability was investigated in two anaerobic completely stirred tank reactors with a working liquid volume equal to 6 litres. The reactors were fed with mixtures containing (on volatile solids basis): 40% of sewage sludge, 30% of organic fraction of municipal waste and 30% of grease trap sludge. The change of hydraulic retention time did not significantly affect process stability. However, methane yields as well as volatile solids removal decreased from 0.54 to 0.47 l per kg of added volatile solids and 65% to 60% respectively, with the decrease of hydraulic retention time. Despite the fact that the best process performance was achieved for hydraulic retention time of 20 days, the obtained results showed that it is also possible to carry out the co-digestion process at shorter hydraulic retention times with good results. Furthermore, gas production rate as well as biogas production at the shortest hydraulic retention time were approximately 46% higher in comparison to results obtained at the longest hydraulic retention time. In this context, the proposed solution seems to be an interesting option, because it provides an unique opportunity for wastewater treatment plants to improve their profitability by enhancing energy recovery from sludge as well as full utilisation of the existing infrastructure and hence creates a new potential place for alternative treatment of organic industrial waste such as: fat-rich materials or food waste. However, implementation of the solution at wastewater treatment plants is still a big challenge and needs studies including identification of optimal digesting conditions, information about substrate pumping, inhibition thresholds and processing properties. Additionally, due to the characteristics of both co-substrates their introduction to the full-scale digester should be carefully planned due to a potential risk of overloading of the digester. For this reason, a gradual increase of the share of these wastes in the co-digestion mixture is highly recommended, because it will allow for the acclimatization of bacteria as well as prevent overloading. The results of this study show the importance of gradual acclimatization of microorganisms to the changing environmental conditions. It was found that concentration of long chain fatty acids in effluents increased with the reduction of hydraulic retention time, but this phenomenon did not significantly influence the performance and stability of the process probably due to changes hydraulic retention time being gradual. Although for palmitic acid a moderate negative correlation with volatile solids removal was observed.

Influence of Seawater to the River Water Quality in Kalibuntung Estuary Southeastern Coast of Surabaya City Indonesia

This study reports distribution of water quality parameters, i.e. salinity, nitrite, nitrate, and phosphate of the Kalibuntung estuary in the southeastern Surabaya coastal area. The study was conducted over 34 sampling points in the river channel, river mouth, and in the coastal water of the Kalibuntung estuary. The result shows that the minimum salinity of (2-4 ppt) reached 3,000 m far from the coastline to upper strem. The maximum salinity of 28.60 ppt found in the coastal water, about 1,000 m seaward from coastline. Based on the numerical modeling analyses, distribution of salinity followed the water movement of eeb-flood currents cycles due to tidal fluctuation. The result obviously revealed that during flood current, water mass from the sea moves to the upper river, on the other hand, during eeb current more volume of river water flows to the sea. It is indicated by salinity values in the river mouth, which reached 27 ppt during high tide and < 20 ppt during low tide. Concentration of ammonia ranging from 0.42 mg/l in the lower part close to the river mouth, up to 6.13 mg/l in the uppermost of the river. This indicated that the river is heavily polluted by organic waste. Concentration of phosphate range from 0.06 to 0.43 mg/l. Its concentration decrease seaward, it is distributed to a distance of 3,000 m fom the river mouth. The river water of Kalibuntung estuary is not permitted to be used as a drinking water, and also it is not feasible for coastal aquaculture. Only in the lower part of the study area where it close to the rivermouth, its water can be used as a source of the coastal aquaculture. This research also found that the river water of the study area has already heavily polluted by organic waste, which it might come from domestic waste, agriculture or fertilizer for fishpond, or industrial organic waste.

Chromium Adsorption in Different Mineralogical Fractions from Subtropical Soils

Safe application of chromium (Cr)–containing organic industrial wastes to soil requires considering the ability of the soil to adsorb Cr. In this study, the maximum Cr adsorption capacity was assessed for the bulk samples and their clay and iron–free clay fractions of four subtropical soils differing in mineralogy. To this end, the samples were supplied with Cr(III) nitrate solutions at pH 4.5 or 5.5. The results of Cr(III) adsorption fitted to a Freundlich equation and the adsorption capacity was positively correlated with soil organic matter and iron oxide contents. The clay fractions adsorbed more Cr per unit mass than the bulk soils and the iron–free clay fractions. The Cr(III) adsorption capacity increased with increasing soil pH due to more charges on adsorbing surfaces. Our results suggest that the soils rich in organic matter and iron oxides and having a pH above 4.5 are suitable for application of Cr(III)–loaded industrial wastes.

Use of Worm meal as Animal Protein Source in Fish Feed

Aquaculture keeps topics of rapid increase in manufacturing amount, economy of feed additives, alternative feed additives, and sustainable supply of feed additives on the agenda. Discontinuity in supply of fish meal being fundamental animal protein source in fish-feed accelerated pursuit for alternative animal protein sources. Earthworms are preferred for the use of domestic and industrial organic wastes as fertilizer in agricultural areas in recent years. Earthworm is a significant animal proteinsource which emerges as final product in this manufacturing. The use of worm meal, which has a significant value in terms of essential amino acid in aquaculture. Total aquaculture production of Turkey in 2015 reached up to 240.334 ton which reveal the increasing need towards animal protein source being a significant component of fish-feed. The aim is to attract attention to the need of researching the effect of worm meal on aquaculture performance. In addition to the use of worms and worm meal in domestic animal feed, research on the use of them in fishfeed also increases rapidly. In this review, importance of the worm and worm meal, and researches conducted with usage potential in fish-feed were discussed. Some resolution advisories for sustainable growth in the amount of aquaculture were presented via worm meal in fish-feed.

Low-carbon agriculture in South America to mitigate global climate change and advance food security

The worldwide historical carbon (C) losses due to Land Use and Land-Use Change between 1870 and 2014 are estimated at 148 Pg C (1 Pg=1billionton). South America is chosen for this study because its soils contain 10.3% (160 Pg C to 1-m depth) of the soil organic carbon stock of the world soils, it is home to 5.7% (0.419 billion people) of the world population, and accounts for 8.6% of the world food (491milliontons) and 21.0% of meat production (355milliontons of cattle and buffalo). The annual C emissions from fossil fuel combustion and cement production in South America represent only 2.5% (0.25 Pg C) of the total global emissions (9.8 Pg C). However, South America contributes 31.3% (0.34 Pg C) of global annual greenhouse gas emissions (1.1 Pg C) through Land Use and Land Use Change. The potential of South America as a terrestrial C sink for mitigating climate change with adoption of Low-Carbon Agriculture (LCA) strategies based on scenario analysis method is 8.24 Pg C between 2016 and 2050. The annual C offset for 2016 to 2020, 2021 to 2035, and 2036 to 2050 is estimated at 0.08, 0.25, and 0.28 Pg C, respectively, equivalent to offsetting 7.5, 22.2 and 25.2% of the global annual greenhouse gas emissions by Land Use and Land Use Change for each period. Emission offset for LCA activities is estimated at 31.0% by restoration of degraded pasturelands, 25.6% by integrated crop-livestock-forestry-systems, 24.3% by no-till cropping systems, 12.8% by planted commercial forest and forestation, 4.2% by biological N fixation and 2.0% by recycling the industrial organic wastes. The ecosystem carbon payback time for historical C losses from South America through LCA strategies may be 56 to 188years, and the adoption of LCA can also increase food and meat production by 615Mton or 17.6Mtonyear−1 and 56Mton or 1.6Mtonyear−1, respectively, between 2016 and 2050.

Effects of co-substrate on biogas production from cattle manure: a review

This literature review surveys the previous and current researches on the co-digestion of anaerobic processes and examines the synergies effect of co-digestion with cattle manure. Furthermore, this review also pays attention to different operational conditions like operating temperature, organic loading rate (OLR), hydraulic retention time (HRT), chemical oxygen demand (COD) and volatile solid (VS) removal efficiency and biogas or methane production. This review shows that anaerobic mono-digestion of cattle manure usually causing poor performance and stability. Anaerobic studies were generally performed under mesophilic conditions maintained between 35 and 37 °C. Organic waste loading rate generally ranges from 1 to 6 g VS–COD L−1 day−1 stable condition in anaerobic digester. Generally, studies show that HRT for co-digestion of fruit–vegetables waste and industrial organic waste appears to exceed 20 days. However, the anaerobic co-digestion process is generally operated at HRT of between 10 and 20 days. VS and COD removal efficiency usually reaches up to 90 % due to co-digestion with different type organic waste. Methane–biogas production is generally obtained between 0.1 and 0.65 L CH4–biogas g−1 VS.

Anaerobic co-digestion of agro-food waste mixtures in a fed-batch basis

ABSTRACTThe agro-food industry (including livestock) generates millions of tonnes of waste products. A solution to this sector's waste disposal challenges was explored by a joint treatment model of organic waste products from several industries. An inventory of agro-food industry organic waste streams with high potential for biogas production was carried out in a logistically viable area (Cider Region, Asturias, Spain). Three industries were selected as those with the higher potential for this study: livestock, dairy and beverage. The kinetics of anaerobic degradation and methane production of four mixtures of selected waste streams were investigated. The specific methane production at five different substrate-to-inoculum ratios (0.50, 0.75, 1.00, 1.50 and 2.00) showed a slightly decreasing trend at the higher ratios. Some hints of a synergistic effect have been observed in mixtures with higher content in milled apple waste, while antagonistic symptoms were noted in mixtures mainly composed of dairy wastes. The estimation of fluxes of waste and methane potentials in the Cider Region suggests centralised anaerobic digestion as a sustainable solution for the valorisation of livestock and agro-food wastes generated in this area. Sector-specific waste streams (livestock and agro-food industry) could cover up to 12% of regional total energy demand.

Conversion of injected waste plastics in blast furnace

Operation of several blast furnaces proved that waste plastic (WP) injection makes recycling of industrial and municipal organic wastes and coke saving possible. However, the extent and reaction kinetics of plastic conversion are hardly explored yet. Therefore, a comprehensive study on WP characteristics and conversion behaviour under simulated raceway, bird's nest and shaft conditions, as well as on its interaction with coke, has been undertaken. Numerous analytical, laboratory and pilot facilities have been used. It has shown that despite favourable chemistry, it is hardly possible to reach a high conversion degree of plastics under the raceway conditions. Consumption of unburned residues in coke bed and in shaft is possible. The effect of this phenomenon on coke reactivity has to be considered as well.