Neurodevelopmental processes of pluripotent cells, such as proliferation and differentiation, are influenced by external natural forces. Despite the presence of biogenic magnetite nanoparticles in the central nervous system and constant exposure to the Earth's magnetic fields and other sources, thereisscant knowledge regarding the role of electromagnetic stimuli in neurogenesis. Moreover, emerging applications of electrical and magnetic stimulation to treat neurological disorders emphasize the relevance of understanding the impact and mechanisms behind these stimuli. Here, the effects of magnetic nanoparticles (MNPs)in polymeric coatings and the static external magnetic field (EMF) were investigated on neural induction of murine embryonic stem cells (mESCs) and human induced pluripotent stem cells (hiPSCs). The resultsshowthat the presence of 0.5% MNPs in collagen-based coatings facilitates the migration andneuronal maturationof mESCs and hiPSCs in vitro. Furthermore, theapplication of 0.4 TeslaEMF perpendicularly to the cell culture plane, discernibly stimulates proliferationand guide fate decisions of thepluripotent stem cells, depending on the origin of stem cells and their developmental stage. Mechanistic analysis revealsthat modulation of ionic homeostasis and theexpression of proteins involved in cytostructural,liposomaland cell cycle checkpoint functions provide a principal underpinning for the impact of electromagnetic stimuli on neural lineage specification andproliferation. These findings not only explore the potential of the magnetic stimuli as neural differentiation and functionmodulator but also highlight the risks that immoderate magnetic stimulation may affect more susceptible neurons, such as dopaminergic neurons.