Induced Hind Paw Edema Research Articles

Evaluation of the analgesic and anti-inflammatory activities of Annona senegalensis Pers. (Annonaceae) leaves aqueous extract in rats and mice

Annona Senegalensis Pers. (Annonaceae) is a medicinal plant used to treat many pathologies, including inflammatory diseases and pain. The aim of this study was to investigate the analgesic and anti-inflammatory properties of Annona Senegalensis leaves aqueous extract in mice and rats. The analgesic activity was evaluated using the acetic acid (1%) induced writhing test and formalin (1%) test. The anti-inflammatory activity was performed using the carrageenan and the dextran induced hind paw oedema in rats. The extract induced a significant (p<0.05) and dose-dependent decrease in abdominal contortions compared with control mice. The maximum inhibition was 63.36% at the dose of 200 mg/kg body weight. Only the late phase of formalin induced nociception was significantly inhibited by the extract with a maximum inhibition value of 56.96% at the dose of 200 mg/kg body weight. In the anti-inflammatory investigation, the aqueous extract of the leaves of Annona Senegalensis produced a significant (p<0.05) and dose-dependent decrease in edema induced by carrageenan and dextran. The maximum inhibition was 57.14% obtained at the fifth hour at the dose of 200mg/kg for the carrageenan test. For the dextran test, the maximum inhibition was 59.80% obtained at the second hour at the dose of 200 mg/kg body weight. Our results show that Annona Senegalensis has peripheral analgesic and anti-inflammatory properties. It could therefore be an advantage in alternative medicine. Keywords: Analgesic, Anti-inflammatory, Annona Senegalensis, Rats, Mice

Anti-inflammatory effects of Pikad Tri-phol-sa-mut-than remedy, consisting of dried fruits of Aegle marmelos (L.) Corrêa, Coriandrum sativum L., and Morinda citrifolia L.

Ethnopharmacological relevanceInflammation has been known to possess some essential roles in many diseases, especially those with chronic or severe conditions. Pikad Tri-phol-sa-mut-than, a Thai traditional herbal remedy, has long been used to treat gastrointestinal sicknesses, fever, and severe illness caused by the deformities of Tridosha. In particular, this recipe has also been applied for inflammation-related conditions including gout and rheumatoid arthritis. The Pikad Tri-phol-sa-mut-than recipe consists of dried fruits of three herbs including Aegle marmelos (L.) Corrêa, Morinda citrifolia L., and Coriandrum sativum L. Each of these plant components of Pikad Tri-phol-sa-mut-than exhibited anti-inflammatory activities. However, anti-inflammatory effect of Pikad Tri-phol-sa-mut-than remedy has not been reported. Aim of the studyThe objective of this study was to elucidate the anti-inflammatory activities of Pikad Tri-phol-sa-mut-than extract (TS) against acute and chronic inflammation in rats. Materials and methodsTo study the effects of TS on acute inflammation, ethyl phenylpropiolate (EPP)-induced ear edema, carrageenan- and arachidonic acid (AA)-induced hind paw edema models were carried out. In addition, cotton pellet-induced granuloma formation was performed to specify the inhibitory effects of TS on chronic inflammation. ResultsThe topical application of TS significantly inhibited EPP-induced ear edema in rats. In the carrageenan- and AA-induced paw edema models, the oral administration of TS significantly reduced paw volumes, compared to those of the control groups. In addition, the 7-day oral treatment of TS demonstrated a significant suppressive effect on cotton pellet-induced granuloma formation. ConclusionsThe current study revealed that TS possesses anti-inflammatory activities against acute and chronic inflammation. Our studies support the use of TS in traditional medicine, and the development of TS as a novel natural product for treating diseases associated with inflammation.

Anti-Inflammatory and Analgesic Evaluation of Parinari curatellifolia Methanol Leaf Extract on Albino Rats

Aim: This research was designed to evaluate the anti-inflammatory and analgesic activities of Parinari curatellifolia methanol leaf extract in albino rats.
 Methodology: Phytochemical screening was carried out using standard methods. Anti-inflammatory activity of the extract was done using egg albumin and formalin induced hind paw edema model. Analgesic effect was evaluated using hot plate induced pain and acetic acid induced writhing test. For each model twenty (20) rats were used, divided into five (5) groups of four (4) rats each.
 Results: Parinari curatellifolia revealed the presence of alkaloids, flavonoids, tannins and phenols while steroids, anthraquinone, terpenoids and glycoside were not detected. For the egg albumin induced inflammation, the group treated with the standard drug (indomethacin) and the group that received the highest dose of the extract were significantly lower (P<0.05) than all the other groups with percentage inhibitions at 25.56% and 24.44% respectively there was no significant difference (P>0.05). For the formalin induced anti-inflammatory activity, at the 1st hour, the normal control group had its paw volume significantly different (P<0.05) from the treated groups. This trend was observed at the 2nd, 3rd and 4th hour. The hot plate method results revealed significant increased (P<0.05) in the analgesic activity of PCMLE at 400mg/kg body weight and the drug treated group when the control was compared with the treated groups with percentage inhibition of 34.32% and 52.94% respectively. The acetic acid induced writhing test revealed that the extract at the three doses of 100, 200 and 400 mg/kg body weight, showed a significant (P<0.05) percentage inhibition of 32.31%, 36.92% and 47.69%, respectively compared to negative control.
 Conclusion: This justifies the use of Parinari curatellifolia locally in the management of pain and inflammation.

Anti-inflammatory Activity of Herbal Tablet of Phyllanthus emblica on Carrageenan-induced Paw Edema in Wistar Rats

Aim: In the current investigation, tablet dosage form of hydroalcohol extract of leaves of P. emblica was developed and evaluated foranti-inflammatory activities.
 Methodology: Hydroalcohol extract of leaves of P. Emblica (HPE) was prepared and formulated tablet dosage form. The mechanical properties e.g. hardness, friability, disintegration, and dissolution of tablet were evaluated. Anti-inflammatory activities of HPE (150 mg/kg) and HPE (650 mg) tablets were performed in carrageenan induced hind paw edema in wistar rats.
 Results: The anti-inflammatory activity was found to be significant in carrageenan inducedpaw edema. HPE (150 mg/kg) and HPE tablets (650 mg) were significantly (p < 0.05) reduced the carrageenan induced paw edemaby 66.41% and 69.43% respectively as compared to carragenan control. The % inhibition of standard drug (dichlorofenac sodium) was recorded to be 70.18% after 5 h.
 Conclusion: The study standardised the development of HPE tablet and its anti-inflammatory effect.

Antinociceptive effects of flower extracts and the active fraction from Styrax japonicus

Ethnopharmacological relevanceFlowers from Styrax japonicus sieb. et Zucc. have been used as a Chinese folk medicine to alleviate pain such as toothache and sore throat. Aim of the studyTo testify the analgesic effect of flowers from Styrax japonicus, analyze components of the active fraction, and investigate the mechanism of analgesia. Materials and methodsFlower extracts were obtained by ethanol, petroleum ether and hydrodistillation extraction. Different fractions of ethanol extracts (EE) were isolated by silica gel column chromatography and preparative liquid chromatography. Analgesic effects of EE, petroleum ether extracts (PEE), hydrodistillation extracts (HDE), and fractions of EE were evaluated using hot plate, acetic acid-induced writhing and formalin tests on mice. Components of the active fraction 1 (F1) were determined by the ultrahigh-performance liquid chromatography Q extractive mass spectrometry (UHPLC-QE-MS). Anti-inflammatory and sedative effects involving analgesic mechanisms were evaluated by carrageenan induced hind paw oedema and pentobarbital sodium sleep tests, respectively. In addition, antagonists including naloxone hydrochloride (NXH), flumazenil (FM), SCH23390 (SCH) and WAY100635 (WAY) were used to investigate the possible mechanism of analgesia. Contents of neurotransmitters and relevant metabolites in different brain regions of mice were also quantified by the ultraperformance liquid chromatography with a fluorescence detector (UPLC-FLD). ResultsEE rather than PEE and HDE at medium and high doses (150 mg/kg and 300 mg/kg) significantly prolonged the latency time of the response of mice to the thermal stimulation in the hot plate test. Moreover, EE significantly decreased number of writhes in the acetic acid-induced writhing test, and reduced licking time in both two phases of the formalin test in a dose-dependent manner. The F1 (50 mg/kg) showed effective antinociceptive responses in all mice models. However, fraction 2 (F2) and fraction 3 (F3) at 50 mg/kg performed no analgesic action. Kaempferol-3-O-rutinoside, isorhamnetin-3-O-rutinoside, pinoresinol-4-O-glucoside, forsythin and arctiin were identified from components of the F1. Furthermore, F1 (50 mg/kg) did not significantly affect hind paw oedema of mice induced by carrageenan but significantly shortened sleep latency and increased sleep duration in the pentobarbital sodium sleep test. In addition, the antinociceptive response of F1 was not affected by NXH in two mice models, but significantly blocked by FM and WAY in the hot plate test. In the formalin test, FM avoided the effect of F1 only in the first phase, while the analgesic activity of F1 was totally suppressed by WAY in both two phases. Otherwise, contents of 5-hydroxytryptamine (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) increased significantly in hippocampus and striatum of mice in the F1 group. ConclusionEE from flowers of Styrax japonicus, and F1, the active part isolated from EE, showed significant antinociceptive activities. The analgesic effect of F1 appeared to be related to the sedative effect, partially mediated by the GABAergic system, and highly involved in the serotonergic system. This was the first study confirming the analgesic effect of Styrax japonicus flower, which provided a candidate for the development of non-opioid analgesics.

Delonix Regia Bark Alleviated Inflammation by Stimulating Defense Responses in Rats

ABSTRACT The methanol extract of Delonix regia bark (DMeOH) was investigated for its anti-inflammatory property in kappa-carrageenan (Carr)-induced hind paw edema of rats. DMeOH was orally administered and evaluated as a measure of edema, tissue destruction, and neutrophil migration to the inflammation site. The pro-inflammatory mediators, p-p65 subunit of NFκB, interleukin (IL)-1β, cycloxygenase (COX)-2 and inducible nitric oxide synthase (iNOS) were analyzed. Catalase (CAT) and superoxide dismutase (SOD) enzyme activity assays and malondialdehye (MDA) were quantified. The LC-MS fingerprint of the extract identified 10 polyphenols, of which five were structurally characterized based on their [M-H]− peak, UV-vis absorption compared to published data. Anti-inflammatory studies revealed that pre-treatment with DMeOH (0.5 g kg−1) prior to Carr-injection inhibited paw edema abating tissue destruction with decreased neutrophil infiltration. Carr-injection resulted in increased NO(•-) production, iNOS transcript and p-p65 subunit of NF-κB protein. DMeOH feed inhibited NO(•-) production and iNOS transcript levels. A decreased expression of NF-κB p-p65 subunit, COX-2 and IL-1β at 0.5 g kg−1 b.w oral feed suggest that DMeOH exerted its anti-inflammatory property by inhibiting NFκB activation. An increase in the antioxidant enzyme activities of CAT (78%) and SOD (49%) with reduced MDA levels was recorded.

Evaluation of antioxidant, anti-inflammatory and anticancer activities of diosgenin enriched Paris polyphylla rhizome extract of Indian Himalayan landraces

Ethnopharmacological relevanceTraditional medicinal plants have gained attention as a potential therapeutic agent to combat cancer and inflammation. Diosgenin rich fresh extracts of Paris polyphylla rhizome from Indian Himalaya is traditionally used as wound healing, anti-bleeding, anti-inflammatory and anti-cancer agent by the folk healers. Aim of the studyPresent study was aimed to prepare two types of extracts from Paris polyphylla rhizome of Indian Himalayan landraces – 1. ethanolic extract of Paris polyphylla rhizome (EEPPR) and 2. Diosgenin enriched Paris polyphylla rhizome extract (DPPE), quantification of diosgenin content, and to evaluate their in vitro anti-oxidant, in vivo anti-inflammatory and in vitro cytotoxicity and anti-cancer activities of the DPPE. Materials and methodsDiosgenin content of EEPPR was quantified through GC-MS while diosgenin content of DPPE was quantified through HPTLC, and the diosgenin yield from EEPPR and DPPE were compared. In vitro antioxidant activities of DPPE were performed using DPPH, NOD, RP and SOD assay while in vivo anti-inflammatory activity of DPPE were evaluated in dextran induced hind paw edema in rats. In vitro cytotoxicity and anti-cancer activities of DPPE were evaluated in human breast cancer cell lines (MCF-7, MDA-MB-231), cervical cancer cell lines (HeLa) and Hep-2 cell lines. ResultsEEPPR obtained through cold extraction method using 70% ethanol showed maximum diosgenin content of 17.90% quantified through GC-MS while similar compounds pennogenin (3.29%), 7β-Dehydrodiosgenin (1.90%), 7-Ketodiosgenin acetate (1.14%), and 7 β-hydroxydiosgenin (0.55%) were detected in low concentration, and thus confirmed diosgenin as major and lead phytochemical. However, DPPE obtained through both cold and repeated hot extraction with the same solvent (70% ethanol) showed diosgenin content of 60.29% which is significantly higher (p < 0.001) than the diosgenin content in EEPPR. DPPE demonstrated significant in vitro antioxidant activities by dose-dependently quenched (p < 0.001) SOD free radicals by 76.66%, followed by DPPH (71.43%), NOD (67.35%), and RP (63.74%) at a max concentration of 2 μg/μl of ascorbic acid and test drugs with remarkable IC50 values (p < 0.01). Further, DPPE also showed potent anti-inflammatory activities by dose-dependently suppressed dextran induced paw edema in rats (p < 0.01) from 2 h to 4 h. DPPE suppressed the proliferation of MCF-7, MDA-MB-231, Hep-2 and HeLa cell lines. Maximum activity was observed in MCF-7 cells. The DPPE also induced apoptosis in MCF-7 cell lines as measured by AO/PI and DAPI staining, as well as DNA laddering, cell cycle analysis and phosphatidylserine externalization assay. The growth-inhibitory effect of DPPE on MCF-7 breast cancer cells was further confirmed from the colony-formation assay. DPPE upregulated expression of Bax and downregulated Bcl-2 and survivin mRNA transcripts. ConclusionDPPE obtained through both cold and repeated hot extraction using ethanol showed significantly higher content of diosgenin than the diosgenin content detected in EEPPR. However, diosgenin yield of both the extracts (EEPPR & DPPE) clearly confirmed diosgenin as major and lead phytochemical of Paris polyphylla rhizome of Indian Himalayan landraces. Further, DPPE also demonstrated potent in vitro anti-oxidative and in vivo anti-inflammatory activities and showed in vitro cytotoxicity and significant anti-cancer (apoptosis) effects in MCF-7 breast cancer cells.

Kirenol, darutoside and hesperidin contribute to the anti-inflammatory and analgesic activities of Siegesbeckia pubescens makino by inhibiting COX-2 expression and inflammatory cell infiltration

Ethnopharmacological relevanceThe Chinese traditional medicine of Siegesbeckia pubescens Makino (SM), which has the effect of healing rheumatism and promoting joint health, is often used to treat rheumatoid arthritis and ischemic stroke. Aim of the studyTo clarify the mechanisms underlying the anti-inflammatory and analgesic influence of active components in the ethanol extract of Siegesbeckia pubescens Makino (ESM). Materials and methodsThe active ingredients in the ESM were identified practicing high-performance liquid chromatography-diode array detection (HPLC-DAD). Four models including xylene-induced ear oedema, complete Freund's adjuvant (CFA)-induced hind paw oedema, acetic acid-induced pain writhing and lipopolysaccharide (LPS)-induced RAW264.7 cell migration, were used to clarify the anti-inflammatory and analgesic mechanisms of the active ingredients in the ESM. Results(1) Three active ingredients of kirenol, darutoside and hesperidin were identified in the ESM, with relative proportion of 0.6%, 0.2% and 0.01%, respectively; hesperidin was reported for the first time in the ESM. (2) Both the ESM and its active ingredients could effectively alleviate the degree of swelling of the auricle and toes, increase the threshold of heat pain, decrease the overexpression of inflammatory protein cyclooxygenase-2 (COX-2) in the skin tissue of the tested parts of the toes, and reduce the number of writhes induced by acetic acid in mice. (3) ESM and its active ingredients also dose-dependently inhibited the migration of RAW264.7 cells. ConclusionsESM and its active ingredients can effectively attenuate the expression of inflammatory factors induced by chemical inflammation, prevent the infiltration of inflammatory cells, and exert good anti-inflammatory and antinociceptive activities.

Effects on anti-inflammatory, DNA binding and molecular docking properties of 2-chloroquinolin-3-yl-methylene-pyridine/pyrazole derivatives and their palladium(II) complexes

Two new sets of Schiff bases 2-chloroquinolin-3-yl-methylene-pyridin-2-amine (CMPA) and 2-chloroquinolin-3-yl-methylene-pyrazole-5-amine-3-thole (CMPT) and their respective palladium (II) complexes have been synthesised and characterized with the aid of elemental analysis, IR, 1H &13C NMR, UV–Vis., and electrochemical studies. The surface morphology of palladium complexes were examined by Scanning Electron Microscopic image. The binding affinities of complexes with CT-DNA were carried out by absorption spectra and cyclic voltammetric studies. The observed hypochromic (~20%) and bathochromic (~30%) shifts indicates that the complexes bind with Guanine base pair of CT-DNA via intercalation. The increasing cathodic peak potential from +0.968 eV to +1.104 eV in complexes confirm the presence of intercalation. Anti-inflammatory activities of both ligands and complexes have been studied using carrageenan induced hind paw edema in Wistar rats. The change in paw volume revealed that the maximum percentage of inhibition was observed in metal complex at 5th hour with a dose of 200 mg/kg. In order to evaluate the binding affinity of ligand and metal complex, molecular interaction analysis were performed by maestro implemented in Schrodinger.

A Comparative Evaluation of Ficus Religiosa with Ficus Species for Its Anti-inflammatory Activity: a Review

Herbal medicine is one of the oldest forms in medical treatment. Through various studies it was known that medicinal herbs are used for treating inflammatory disorders in traditional medicine. Inflammation is the body’s process of opposing against the foreign particles that harm it, such as infections, injuries etc. The work aims to compare the anti-inflammatory action of extracts of various Ficus species using Carrageenan induced hind paw oedema method and cotton pellet granuloma method. Literature surveys have been done to check the extent of anti-inflammatory activity on extracts of various plant parts like roots, leaves, barks, fruits etc. of Ficus species with oral administration of different doses. As a result, the leaf extract of plant Ficus religiosa have found to be greater effect compared to other Ficus species. Thus, the review may conclude that the anti-inflammatory effect of Ficus religiosa extract is more potent than other Ficus species

Anti-Inflammatulatory Activity of Hydro-Ethanolic Bark Extracts of Piliostigma reticulatum Hochst (Caesalpiniaceae) and Analysis by HPLC-DAD and HPLC-MS of its Methanol Fraction

Use of plants as remedies dates back to the time of the oldest civilizations. Among these active ingredients, polyphenols play an important role. Piliostigma reticulatum, a plant whose barks are rich in condensed tannin is often used as an anti-inflammatory. The purpose of this work was to correlate the anti-inflammatory activity of extracts with their chemical composition through chromatography fractionation analyses (HPLC-DAD and HPLC-MS). The barks were extracted by moderate hydroethanol decoction followed by silica gel splitting with successively ethyl acetate, methanol and water. Anti-inflammatory activity was evaluated by the method of carrageenan induced hind paw edema in Wistar rats. The chemical study was carried out by HPLC-DAD and HPLC-MS after isolation of the compounds by fractionation on Sephadex LH-20 gel. The bark hydroethanolic extract had shown ability to inhibit significantly the inflammation induced by carraghenan. Among the hydroethanolic bark extract fractions that methanol fraction had the best activity at 10 mg/kg with inhibition percentages similar to those of acetylsalicylic acid with 48.51% ± 2.26; 54.71% ± 5.13; 64.19% ± 6.70 vs. 54.69% ± 7.23; 57.83% ± 9; 65.13% ± 8.44 respectively. The HPLC-DAD analysis showed that the SF5 sub-fraction presented the best chromatogram with several peaks, three of which were high intensity. The latter would correspond to monomer, dimer and trimer of catechin according to the weights [M-H] + m / z: 291.07; 579.18; 867.27 obtained by HPLC-MS. The compounds responsible for anti-inflammatory activity would be condensed tannins. The latter would consist mainly of catechin oligomers.&#x0D; Keys words: Piliostigma reticulatum, bark, anti-inflammatory activity, HPLC-DAD, HPLC-MS

Pharmacological Screening for CNS Depression, Analgesic and Anti-inflammatory Potentials of Sonneratia caseolaris (Linn.) Barks in Different Solvent Fraction

Aims: Bark of different fractions of Sonneratia caseolaris (Linn.) (Sonneratiaceae) were screened for its analgesic, anti-inflammatory and CNS activities.&#x0D; Study Design: For the purpose of these experiments the extracts were subjected to an in-vivo study.&#x0D; Place and Duration of Study: The study was carried out in August 2014 in the Department of Pharmacy, Southeast University, Dhaka, Bangladesh.&#x0D; Methodology: Ethanolic (ETF), ethyl acetate (EAF), chloroform(CLF) and pet ether (PTF) fractions of bark of S. caseolaris were used to evaluate the analgesic activity using Acetic acid induced writhing and Formalin test. The same fractions were evaluated for anti-inflammatory activity using Carrageenan induced hind paw edema model. The CNS depressant activity was evaluated by Hole cross method. Two doses of 150 mg/kg and 300 mg/kg were used.&#x0D; Results: The different fractions produced significant (p&lt;0.05) writhing inhibition at both doses and reduced the number of linking induced by formalin. Among these fractions the most potent activity was found in ETF about 79.40% (300 mg/kg) that was almost similar to standard Diclofenac-Na 82.78% (10 mg/kg), then EAF 74.59% followed by CLF 59.03% and PTF 52.45% at dose 300 mg/kg).&#x0D; In formalin-induced paw licking model, all fractions of S. caseolaris showed superior result in the late phase compare to the early phase .The same fractions of extracts caused significant (p&lt;0.05) inhibition of carrageenan induced paw edema in a dose dependent manner. A statistically significant (p&lt;0.05) locomotor activity was also observed.&#x0D; Conclusion: Our result revealed that all the extractives of S. caseolaris have noticeable analgesic, anti-inflammatory and CNS depressant activities.

Albizia anthelmintica: HPLC-MS/MS profiling and in vivo anti-inflammatory, pain killing and antipyretic activities of its leaf extract

In the current work, the phytochemical composition of a leaf methanol extract from Albizia anthelmintica was thoroughly investigated. The antioxidant, anti-inflammatory, analgesic, and antipyretic activities of the extract were investigated. In the carrageenan induced hind paw edema bioassay; the extract significantly reduced the edema thickness in rats and diminished the leukocyte migration to the peritoneal cavity in mice. The extract exhibited central and peripheral anti-nociceptive effects; it significantly decreased the number of acetic acid induced writhes and prolonged the latency time in the hot plate test. The extract showed a substantial antipyretic activity as it decreased significantly the elevated rectal temperature in mice after intraperitoneal injection of Brewer’s yeast. Molecular docking of some major compounds in the extract to COX-1, COX-2 and 5-LOX, enzymes involved in the inflammation cascade, revealed appreciable interactions with the conserved amino acid residues in these target proteins. These findings were confirmed with in vitro enzyme inhibitory assays in which the extract showed IC50 values of 4.11, 0.054, and 1.74 μg/mL towards COX-1, COX-2 and 5-LOX, respectively. The extract displayed solid antioxidant properties as well with a TAC value of 35.13 U/L and EC50of 5.36 μg/mL in DPPH assay. These findings suggested that Albizia anthelmintica is a good antioxidant with potential therapeutic efficacy for treating inflammation, pain and related oxidative stress disorders.

Evaluation of wound healing and anti-inflammatory activity of the leaves of Calpurnia aurea (Ait.) Benth (fabaceae) in mice

Abstract Ethnopharmacological relevance The leaves of Calpurnia aurea (Ait.) Benth are used for wound healing activities through topical application in different communities in Ethiopia. The leaves had many confirmed in vitro activities that can promote wound healing effects. In spite of many claims and in vitro studies with supportive results in wound healing, no scientific study has been conducted on the wound healing and anti-inflammatory activity of leaves of Calpurnia aurea (Ait.) Benth on animals. Aim of the study was to evaluate the wound healing and anti-inflammatory activity of leaves of Calpurnia aurea (Ait.) Benth scientifically in mice. Methods and materials Air dried leaves of Calpurnia aurea (Ait.) Benth were grounded and macerated three times successively by 80% methanol. Then part of the dried extract was fractionated with chloroform, ethyl acetate and aqueous. Phytochemical screening tests were performed according standard test procedures. The crude extract and solvent fractions were formulated to ointments. The acute dermal toxicity of the crude extract was determined in rats. Wound healing activity of the crude extract was evaluated using excision and incision wound models and the wound healing activities of solvent fractions were evaluated by using excision wound model. The anti-inflammatory activity of the 80% methanol extract of leaves of Calpurnia aurea (Ait.) Benth was evaluated using carrageenan induced hind paw edema model in mice. Results The 2000 mg/kg test dose of the 10% w/w crude extract ointment was safe in rats. The 5% w/w and 10% w/w crude extract ointments revealed significant (p Conclusion The results of this study demonstrated that 80% methanol extract of the leaves of Calpurnia aurea (Ait.) Benth exhibited wound healing and anti-inflammatory effects. The aqueous and ethyl acetate fractions possessed wound healing activities.

Phytochemical analysis, antioxidant, antidiabetic and anti-inflammatory activity of bark of Gardenia latifolia

Gardenia latifolia (Rubiaceae) is commonly known as Indian boxwood or Ceylon boxwood, is a densely foliaceous small tree that occurs throughout the greater parts of Indian common in deciduous forests along the streams. The stem bark and fruits are reported to be used in the treatment of various ailments such as snake bite, skin diseases, stomach pains, caries in humans and ephemeral fever in live stocks. Many beneficial medicinal products are used to treat various serious diseases and disorders like diabetes, cancer and cardiovascular diseases without side effects. Hence our study focused to investigate the phytochemical analysis, quantification of bioactive compounds, in vitro free radical scavenging activity (DPPH radical method), anti-inflammatory activity (Carrageenan induced hind paw oedema ), in vitro anti-diabetic activity by enzyme inhibition activity of bark of Gardenia latifolia (hydroalcoholic extract) which has boundless medicinal properties. The results of this study showed the evidence that the extracts when tested for their phytochemicals and free radical scavenging activity were found to have considerable antioxidant potential. This plant also exhibit better in vitro enzyme inhibitory activity and anti-inflammatory activity. The results of this study indicate that the hydroalcoholic extract of Gardenia latifolia bark has significant pharmacological properties.&#x0D; Keywords: Gardenia latifolia, Phytochemical analysis, DPPH radical method, Anti-inflammatory activity, Anti-diabetic activity

Comparative evaluation of anti-inflammatory activity of Manahshila (Realgar)

Background: Realgar popularly known as Manahshila (in Ayurveda) is an arsenic and sulphur containing mineral drug. The Manahshila is used after Shodhana (purification) for the treatment of various diseases in traditional Indian systems of medicine which also includes pain related disorders. Aim: This study is aimed to evaluate the comparative anti-inflammatory efficacy of Manahshila using in vivo models of inflammation in rats. Materials and Methods: In the present study, Shodhana of Manahshila is performed by the Bhavana (levigation) where trituration of Manahshila is done with ginger (Zingiber officinale Rosc.) juice, sesbania leaves (Sesbania grandiflora Poir.) juice, lemon (Citrus medica Linn.) juice and lime water. The anti-inflammatory activity of the Manahshila at 0.35, 0.7 and 1.4 mg/kg, p.o. was evaluated by egg albumin induced hind paw edema in rats. Results: The Ashodhita Manahshila (crude realgar) showed mortality while Shodhita Manahshila (purified with all 4 media) did not showed mortality in the treated rats up to a dose of 2,000 mg/kg. Ashodhita and Shodhita Manahshila revealed that at the dose level of 1.4 mg/kg it showed inhibition (p < 0.05) of egg albumin-induced hind paw edema. But Shodhita Manahshila showed anti-inflammatory activity at all three dose level in a dose dependent manner. The ginger juice treated Manahshila (56.60%) was found best alleviates rat’s response to egg albumin induced inflammation among all group. Conclusion: The results validate its usage in the healing of inflammation and also give support to its perspective as the source of novel pain relief medicine prototype.

Anti-Inflammatory Activities and Related Mechanism of Polysaccharides Isolated from Sargentodoxa cuneata.

Sargentodoxa cuneata decoction has been used to treat arthritis in China for hundreds of years. Herein, the polysaccharide fraction (PSC) purified from S. cuneata was evaluated for its in vitro and in vivo anti-inflammatory effects. PSC and its sub-fractions PSCA-1 and PSCB-1 significantly suppressed nitric oxide (NO) release in LPS-induced RAW264.7 cells by down regulating the inducible nitric oxide synthase (iNOS) level. Furthermore, PSC markedly inhibited carrageenan induced rat hind paw edema, decreased in hind paw, serum and liver malondialdehyde (MDA) levels and prostaglandin E2 (PGE2 ) levels. In addition, PSC increased superoxide dismutase (SOD) activity in serum and liver of the rats. These results revealed that the polysaccharide obtained from S. cuneata (PSC) possessed potent anti-inflammatory activity and may be one of the important bioactive constituents from the plant responsible for the anti-arthritis effect.

Analgesic and anti-inflammatory activity of ethanolic extracts of leaf and fruit pulp of Aegle marmelos in albino rats

Background: To study the analgesic and anti-inflammatory activity of the ethanolic extracts of leaf (LE) and fruit pulp (FE) of Aegle marmelos in comparison with the standard drugs pentazocine and diclofenac in albino rats. 56 healthy wistar albino rats of either sex were randomly divided into 14 groups of 4 each (n=4), weighing about 150-200grams were selected for the study. All drugs i.e. 1ml of 1% Carboxy methyl cellulose (CMC), pentazocine 10mg/kg (intraperitoneal), diclofenac 10 mg/kg and LE and FE at doses of 100mg/kg and 200mg/kg in 1% CMC (vehicle) were administered per oral one hour before the experiment.Methods: Analgesic activity was assessed by Eddy’s hot plate method (latency time) and acetic acid induced writhing (number of writhings) method. Anti-inflammatory activity was assessed by carrageenan induced hind paw edema method using digital plethysmometer.Results: Test drugs at doses LE 200mg/kg and FE 200mg/kg have shown significant increase in mean latency time in Eddy’s hot plate (P &lt;0.001) and decrease in the average number of writhing movements (P &lt;0.01). LE 200 and FE 200 doses did not show significant anti-inflammatory activity in Carrageenan induced paw edema model (P &gt;0.05).Conclusions: Leaf extract (LE) and fruit extract (FE) at doses 200mg/kg have shown significant analgesic action and no anti-inflammatory action.

Synthesis, characterization and pharmacological evaluation of substituted 4-aryl thiazole-2-amino acetanilides

A series of novel 4-aryl thiazole-2-amino acetanilides (B1-B6) was designed and synthesized via the preparation of 2-amino 4-aryl thiazole (1) by acetophenone and thiourea which on subsequent condensation with α-chloroacetanilides offered title compounds. The newly synthesized compounds were characterized by elemental analysis, IR and 1HNMR and Mass spectral data. The synthesized compounds were tested for their in-vitro antimicrobial activity against the four bacterial strains i.e., Bacillus subtilis, Streptococcus pneumonia, Escherichia coli and Staphylococcus aureus and two fungal strains i.e Aspergilus. Niger and Candida albicans using disk diffusion method. The synthesized derivatives demonstrated moderate to excellent growth inhibition of microbes. The compounds were also tested for their antiinflammatory and analgesic potential using carageenan induced hind paw oedema and acetic acid induced writhing methods respectively. A promising activity was observed in some of the title compounds when compared to standard drug diclofenac sodium.

Pain &amp; Inflammation: Effects of short term daily adminstration of Vitamin B12 &amp; Folic acid in long evans rats

Background : Vitamin B12 &amp; Folic acid (FA) are used with other B vitamins to relieve various painful and inflammatory conditions. But combined effects of vitamin B12 &amp; FA on nociceptive pain, inflammatory pain and inflammation are yet to be clearly demonstrated.Objective: To assess the effectiveness of short term daily administration of B12 &amp; FA on reducing pain and inflammation.Methods: This prospective experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka from 1st January 2011 to 30th June 2012. For this purpose, 12 male Long Evans rats, weighing 200 to 250 grams were collected from the animal house of BIRDEM, Shahabag, Dhaka. All the rats received a daily intraperitoneal injection of either combination of B12 (15mg/kg) &amp; FA (5mg/kg) or equal volume of normal saline for 7 consecutive days. To evaluate the effects on pain, tail immersion test for nociceptive pain and formalin test for nociceptive &amp; inflammatory pain were done. In addition, to evaluate their effects on inflammation formalin induced hind paw oedema was measured.Results: Combination of B12 &amp; FA Supplementation significantly lowered the variables for inflammatory pain &amp; inflammation.Conclusion: This study revealed that, combined short term daily supplementation of B12 &amp; FA is effective in lowering inflammatory pain &amp; inflammation.Bangladesh Crit Care J March 2016; 4 (1): 33-37