The slow temperature acclimation of photosynthesis has been confirmed through early field experiments and studies. However, this effect is difficult to characterize and quantify with some simple and easily accessible indicators. As a result, the impact of slow temperature acclimation of photosynthesis on gross primary production (GPP) estimation has often been overlooked or not integrated into most GPP models. In this study, we used a theorical variable-state of acclimation (S), to characterize the slow temperature acclimation. This variable represents the temperature to which the photosynthetic machinery adapts and is defined as a function of air temperature (Ta) and time constant (τ) required for vegetation to respond to temperature, to discuss its impact on GPP simulation. We used FLUXNET2015 dataset to calculate S and established a GPP model using S and shortwave radiation (SW) based on random forest algorithm (S model). As a comparison, we directly used Ta and SW to build the other GPP model (Ta model). Moreover, the divergent temperature acclimation capacities of plants are crucial to predict and make preparations for likely temperature stress in the future. Therefore, the spatial distribution of τ values was also mapped using satellite sun induced chlorophyll fluorescence (SIF) and Ta datasets. The results indicated that: (1) taking into account the slow temperature acclimation of photosynthesis led to a more precise estimation of GPP which mainly reflected in reduction of excessive fluctuations in GPP predictions; (2) considering the slow temperature acclimation of photosynthesis can reduce the sensitivity of vegetation to temperature; (3) the improvement of S model in GPP estimations was different in different vegetation growth stages which was more significant in the springtime recovery stage; (4) τ values had significant spatial distribution which was strongly affected by the determinants of vegetation growth and seasonal variations in temperature.