Effects of UV radiation on photosynthesis of Sargassum muticum

Abstract

As an invasive species of macroalgae, Sargassum muticum (Yendo) Fensholt has invaded European shores and the Mediterranean, and even drifting individuals have been observed in sea area near the Canary Islands, becoming a potential species responsible for the “Sargassum golden tide”. When drifting on the sea surface, algae receive more ultraviolet radiation (UVR). However, photosynthetic responses and adaptation mechanism of S. muticum to UVR remain unclear. In this study, thalli of S. muticum were respectively exposed under two radiation treatments of 200 W m−2 PAR (photosynthetically active radiation, 400–700 nm), and 200 W m−2 PAR + 38 W m−2 UVR (280–700 nm) for 120 mins, and then recovered at a low light (PAR) of 10 μmol photons m−2 s−1 for 240 mins. During this treatment, the photosynthetic properties of algae were measured, to investigate the responses and protection mechanism of S. muticum to UVR. The results showed that photosynthesis of thalli was significantly inhibited by UVR, reflected by decreased maximum photochemical quantum yield (Fv/Fm) and chlorophyll content, and changes of parameters of rapid light response curve and chlorophyll fluorescence induction curve in PAR and PAR + UVR treatments. Meanwhile, algae actively adopted several strategies to alleviate the inhibition caused by UVR, including photoprotection characterized by increased contents of UV-absorbing compounds (UVACs) and carotenoids (Car), excess light energy dissipation by elevated non-photochemical quenching (NPQ) coupled to the xanthophyll cycle, and photorepair manifested as an accelerated synthesis of D1 protein and enhanced antioxidant for scavenging ROS. Benefiting from the effects of the above several pathways, after 240 mins of low light recovery, photosynthesis was greatly restored in these two radiation treatments, with the restoration of Fv/Fm and parameters of rapid light response curve and chlorophyll fluorescence induction curve. Based on these results, we conservatively speculate that the photosynthesis of drifting S. muticum individuals may be restored in the evening with low light levels after being damaged by UVR on the surface of sea at midday, resulting in maintaining rapid growth and forming a golden tide.