Induce Skin Sensitization Research Articles

Acute and Subacute Toxicology and Safety Evaluation of Triphenyl Tin Hydroxide (Vancide KS)

The acute oral LD50 in Sprague-Dawley rats was determined to be 171 mg/kg (100-295) for males and 268 mg/kg (205-344) for females. 2. A 1 ppm dietary supplement of Vancide KS for 90 days did not induce any abnormalities in weanling Sprague-Dawley rats. The parameters evaluated were serum glutamic-oxaloacetic transaminase (SGOT), hematocrit, differential white blood cell count, food consumption, and weight gain, along with histologic studies of the myocardium, spleen, liver, kidney, stomach, and small intestine. A 500 ppm diet was lethal. Weanling and older rats subjected to 1000 and 10,000 ppm diets died within 5 days. 3. Vancide KS induced no acute dermal toxicity, nor did it exhibit percutaneous absorption in New Zealand strain albino rabbits. 4. Vancide KS induced no chronic dermal toxicity in New Zealand strain albino rabbits. 5. Vancide KS was not shown to be teratogenic. It exhibited an antifertility action, especially in Sprague-Dawley rats dosed on day 1 through day 7 of timed-pregnancy. 6. Vancide KS was shown to be an eye irritant which induces corneal opacity. 7. Acute oral toxicity studies in Sprague-Dawley rats indicate that Vancide KS should be classified as a toxic substance as defined in the regulations under the Federal Hazardous Labeling Act. 8. The intravenous administration of 25 mg/kg of Vancide KS to New Zealand strain albino rabbits induced death preceded by topic convulsions. 9. Vancide KS did not induce skin sensitization in male adult guinea pigs.

Toxicity and Metabolism of 2-Hydroxy-4-n-octoxybenzophenone

2-Hydroxy-4-n-octoxybenzophenone (UV 531) is a stabilizer for synthetic resins. Toxicity and metabolism studies were conducted to evaluate its safety as a component of plastics used in contact with food. The acute oral LD50 of UV 531 for male albino rats and the LD50 by continuous 24-hr contact with the clipped skin of male albino rabbits are both greater than 10 g/kg. The substance is not irritating to rabbit skin or rabbit eyes, and shows no detectable capacity for inducing skin sensitization in man. Albino rats of both sexes tolerated a dietary level of 1·8% UV 531 for 90 days with no effect other than a significant increase (P<0·05) in mean relative kidney weight in males. Rats fed 0·6% or less of the product did not differ from their controls. A diet containing 1·8% UV 531 was unpalatable to dogs, but dietary levels of 0·6% or less had no adverse effect on the dogs during a 120-day test period. Preliminary metabolic studies with male rats indicated that most of the orally-administered UV 531 could be recovered unchanged from the faeces. The small portion that was absorbed was excreted as a glucuronide conjugate in the urine. Consequently, groups of male rats were fed a diet containing 1·25 or 5·0% of UV 531 for periods of up to 30 days. Daily records were kept of UV 531 intake, and urine and faeces were collected separately each day. The metabolic pattern was the same at each dietary level. The mean daily recovery of UV 531 from the faeces was about 90%, and the mean daily urinary excretion of UV 531, conjugated as a glucuronide, was about 10%. The total amount of ingested UV 531 appears to be accounted for by these two forms of excretion.

Toxicity and metabolism of 2-hydroxy-4- n-octoxybenzophenone

2-Hydroxy-4- n-octoxybenzophenone (UV 531) is a stabilizer for synthetic resins. Toxicity and metabolism studies were conducted to evaluate its safety as a component of plastics used in contact with food. The acute oral LD 50 of UV 531 for male albino rats and the LD 50 by continuous 24-hr contact with the clipped skin of male albino rabbits are both greater than 10 g/kg. The substance is not irritating to rabbit skin or rabbit eyes, and shows no detectable capacity for inducing skin sensitization in man. Albino rats of both sexes tolerated a dietary level of 1·8% UV 531 for 90 days with no effect other than a significant increase ( P<0·05) in mean relative kidney weight in males. Rats fed 0·6% or less of the product did not differ from their controls. A diet containing 1·8% UV 531 was unpalatable to dogs, but dietary levels of 0·6% or less had no adverse effect on the dogs during a 120-day test period. Preliminary metabolic studies with male rats indicated that most of the orally-administered UV 531 could be recovered unchanged from the faeces. The small portion that was absorbed was excreted as a glucuronide conjugate in the urine. Consequently, groups of male rats were fed a diet containing 1·25 or 5·0% of UV 531 for periods of up to 30 days. Daily records were kept of UV 531 intake, and urine and faeces were collected separately each day. The metabolic pattern was the same at each dietary level. The mean daily recovery of UV 531 from the faeces was about 90%, and the mean daily urinary excretion of UV 531, conjugated as a glucuronide, was about 10%. The total amount of ingested UV 531 appears to be accounted for by these two forms of excretion.