Indonesian Plants Research Articles

Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model

BackgroundThis study evaluated in vitro antigiardial activity in four Indonesian plants (Archidendron fagifolium, Diospyros sumatrana, Piper betle and Shorea sumatrana) extracted in methanol, methanol-tetrahydrofuran, and water. These plants exhibiting promising anti-parasitic activity were selected on the basis of collected behavioral data and their ability to decrease parasite load in Sumatran orangutans. Extracts of Arabidopsis thaliana, a plant routinely used as a laboratory model in research, were used as a negative control.MethodsPlant extracts of different concentrations (400, 100, 25 and 6.25 µg/ml) and metronidazole (100 µg/ml), a standard giardicidal drug, were incubated with 25,000 trophozoites per milliliter of growth medium in 12-well tissue culture plates under anaerobic conditions for 72 h. Cultures were counted in a hemocytometer using a light microscope and then statistically evaluated. The cytotoxicity of the extracts was determined by the MTT Cell Viability Assay.ResultsWe recorded a statistically-significant decrease in giardia trophozoites in two extracts: the methanolic extract of A. fagifolium (Kruskal-Wallis: p = 0.013) and the aqueous extract of P. betle (Kruskal-Wallis: p = 0.002). Effective concentrations of 400 µg/ml and 100 µg/ml, respectively, were revealed. Cytotoxicity was not demonstrated.ConclusionsThe results of our study show that extracts of A. fagifolium and P. betle have potential as an alternative treatment of G. intestinalis infection. This is the first scientific proof of the biological activity of A. fagifolium, confirming the assumption that orangutans use this plant (as well as P. betle) for self-medication.

Therapeutic potential of Indonesian plant extracts in combating malaria and protozoan neglected tropical disease

BackgroundNeglected tropical diseases (NTDs) afflict nearly 2 billion people worldwide and are caused by various pathogens, such as bacteria, protozoa, and trypanosoma, prevalent in tropical and subtropical regions. Among the 17 NTDs recognized by the World Health Organization (WHO), protozoal infections caused by Plasmodium, Entamoeba, Leishmania, and Trypanosoma are particularly prominent and pose significant public health. Indonesia, endowed with a rich biodiversity owing to its tropical climate, harbors numerous plant species with potent biological activities that hold promise for therapeutic interventions. Hence, efforts have been directed towards exploring Indonesian plant extracts and isolated compounds for their potential in combating protozoal diseases.MethodsThis study evaluated the antiprotozoal properties of 48 plant extracts sourced from the Cratoxylum, Diospyros, and Artocarpus genera. These extracts were screened using cell-based assays against Plasmodium falciparum (Pf), Entamoeba histolytica (Eh), Leishmania donovani (Ld), Trypanosoma brucei rhodesiense (Tbr), and Trypanosoma cruzi (Tc).ResultsExtracts derived from the roots of Cratoxylum arborescens, obtained through dichloromethane extraction, exhibited significant activity against protozoa, with an IC50 value ranging from 0.1 to 8.2 µg/mL. Furthermore, cochinchinone C was identified as an active compound capable of inhibiting the growth of Pf, Eh, Ld, and Tbr, Tc trypomastigote, and Tc epimastigote with IC50 values of 5.8 µM, 6.1 µM, 0.2 µM, 0.1 µM, 0.7 µM, and 0.07 µM, respectively. Cochinchinone C is the first compound reported to exhibit activity against protozoal neglected tropical diseases, showing low cytotoxicity with a selectivity index greater than 10 when tested against carcinoma and normal cell lines. This suggests indicating its potential as a candidate for further drug development. This is the first report of cochinchinone C’s activity against these protozoans.ConclusionThese findings establish cochinchinone C as a strong candidate for antiprotozoal drug development, highlighting the untapped therapeutic potential of Indonesia’s rich plant biodiversity.

Pioneering plant metabolomic library of Indonesian plants for research, conservation, capacity building and economic development

Indonesia, one of the world’s most biodiverse countries, is undergoing mass deforestation, exacerbating climate change and leading to accelerated loss of species. This project addressed the urgent need to conserve endangered Indonesian biodiversity, specifically the potentially life-saving bioactive compounds harboured within its plants. A group of Indonesian researchers from Universitas Nasional (UNAS) in Jakarta received training in RApid Metabolome Extraction and Storage (RAMES) technology, an ethical, low-impact, field-deployable and cost-effective methodology developed by Rutgers University. The team of Indonesian scientists used this technology to create the first metabolomic library of Indonesian plant species and an easily transportable collection containing 501 metabolome samples from 296 species. This pioneering and readily shareable resource aims to foster collaborative research into plant metabolomics and natural products, reaching across Indonesia and the broader Southeast Asia region. The project also facilitated four formal discussion forums, two of which were international conferences, promoting exchange among Indonesian, Southeast Asian and USA scientists, with notable participation from the Indonesian National Research and Innovation Agency (BRIN). These efforts culminated in the formation of a strategic partnership among UNAS, BRIN and Rutgers.

The Effect of a Combination of Ethanol Extract from Leaves and Flowers of Plumeria acuminate L. Against Aedes aegypti Larvae

Aedes aegypti is the primary vector that causes dengue virus (DENV) infection. Ae. aegypti resistance to abate (Temephos) has occurred in Indonesia and several other countries. Innovations are needed to develop alternative ingredients that can be used as natural larvacides. The Plumeria acuminate L. is an Indonesian plant with metabolites that have the potential to act as larvicides. This study aims to determine the potential combination of ethanol extract of P. acuminate L. leaves and flowers as a larvicide for A. aegypti. A laboratory experimental study with a post-test-only control group design was carried out on Ae. aegypti larvae instar III. The larvicide test was carried out according to WHO standards in 2005 with concentrations of 10000, 7500, 5000, and 2500 ppm, as well as control (water and 1% DMSO), with 20 larvae for each concentration. The observation was carried out at the 24th and 48th hours with 3 repetitions. Larval mortality data was analyzed using one-way ANOVA statistical tests to determine significant differences and lethal concentrations (LC50 and LC90) were calculated using probit analysis using SPSS software. The mortality of Ae. aegypti larvae due to the administration of a combined ethanolic extract of P. acuminate L. leaves and flowers was the highest at doses of 10000 and 7500 ppm, which was 100% with lethal concentrations of LC50 and LC90 of 3364.715 and 6293.759 ppm at the 24th hour observation, whereas at the 48th-hour observation, lethal concentrations were detected at 1767.998 and 2941.138 ppm, respectively. One-way ANOVA analysis test showed a significant difference in Ae. aegypti larval mortality due to the administration of a combined ethanol extract of P. acuminate L. leaves and flowers at 24th-hour observation (p = 0.000) and (p = 0.013) at 48th hour observation. The combination of ethanol extract of P. acuminate L. leaves and flowers showed larvicidal activity against Ae. aegypti larvae as evidenced by larvae mortality which were influenced by concentration and observation time.

Variability and pharmacological potential of bajakah (Spatholobus sp.) as an indigenous medicinal plant: a review

Bajakah (Spatholobus sp.) is an indigenous Indonesian plant that has been traditionally used as medicine, especially in Kalimantan Island, Indonesia. The variability and potential of bajakah as a traditional Indonesian medicinal plant have attracted research attention. This review article describes the traditional use, distribution, and pharmacological activity of bajakah. An integrative review method was employed, following procedures such as literature search, data analysis, and result presentation. Understanding the variations is important to understand the therapeutic potential and differences in active compound content among different types of bajakah. Furthermore, the chemical composition of bajakah has been identified, including alkaloids, flavonoids, tannins, saponins, and triterpenoids, which can potentially exhibit pharmacological effects and mechanisms of action associated with its traditional use. This review provided additional insights into the potential of bajakah as an herbal medicinal in the future. Therefore, this review article provides an overview of the traditional use distribution, and pharmacological activity of bajakah as a promising traditional medicine. Further research, including clinical studies, is needed to scientifically validate the therapeutic potential of bajakah and further understand the mechanisms of action and potential side effects associated with it.

Particle Size Optimization of Melinjo (Gnetum gnemon L.) Seed Hardshell: A Potential Antioxidant Alternative

Natural ingredients can have extraordinary potential as alternative medicines due to their accessibility and cost-effectiveness. Application of these ingredients should consider solubility and permeability, which determine the success of pharmaceutical characteristics formulation and biological activity indication. In this context, physical manipulation, specifically particle size reduction, is an effective strategy to address these issues. Previous research has explored active compounds in the stilbenoid group, found in the outer skin, hard shell, and endosperm of melinjo (Gnetum gnemon L.) seeds, functioning as antioxidant. Based on the potential as antioxidant, stilbenoid compounds, including resveratrol, contained in melinjo seed hardshell have shown significant pharmacological effects. Therefore, this research aimed to investigate the potential of melinjo seed hardshell extract as a natural antioxidant alternative by modifying the particle size through a grinding process to obtain nanoparticles. The analysis was carried out using ball milling to enhance the solubility of melinjo seed hardshell extract by increasing the saturated solubility and surface area of the particles. The results showed that the total phenol content and the antioxidant power increased significantly (p < 0.05) after ball milling. Melinjo seed hardshell nanoextract is reported herein as a promising source of natural antioxidant from local Indonesian plants.

Antidiabetic Activity and Pancreatic Histopathology Analysis of Averrhoa bilimbi L. Leaves Extract on Alloxan-Induced Diabetes on Wistar Mice

Background: Diabetes mellitus is a multi-etiological metabolic disorder characterized by chronic hyperglycemia, total or relative insulin deficiency, and further complications by metabolic disorders. Drug therapy for diabetes mellitus is costly and fraught with potential side effects. The aim of this research is to investigate antidiabetic and histopathological analysis of ethanolic A. bilimbi leaves extract in alloxan induced diabetic mice.Methods: Wuluh starfruit leaves is one of Indonesia's plants that can be used to treat diabetes mellitus. Thirty male Wistar mice were divided into five groups as follows: Negative control group that didn't receive any treatment, Diabetes control group that received alloxan 150 mg/kg, Positive control group that received glibenclamide 600 μg/kg, and lastly the two test groups that received A. bilimbi leaves extract 150 and 300 mg/kg orally for 14 days. After extract administration, blood glucose and histopathological alterations in the pancreas of diabetic mice were observed.Results: Alloxan to the diabetes control group significantly raised blood glucose levels compared to the negative control group. Administration of wuluh starfruit leaves extract and glibenclamide could significantly lower blood sugar levels compared to the diabetic control group. Administration of wuluh starfruit leaves extract treatment and glibenclamide also showed favorable effect on the histopathological changes of the pancreas in alloxan induced diabetes.Conclusion: The study concludes the administration of wuluh starfruit leaves extract (150 and 300 mg/kg) and glibenclamide could reduce blood glucose levels and can help with cell regeneration and protecting pancreatic cells from damage caused by alloxan.Keywords: A. bilimbi leaves extract; Alloxan; Diabetes; Pancreatic histopathology

Molecular docking and toxicity analysis of ribosome-inactivating protein and natural chemical compounds as promising antiretroviral candidates against HIV-1

Background: HIV (human immunodeficiency virus) uses HIV-1 reverse transcriptase as the receptor to convert RNA into viral DNA. In addition, the CD4 receptor is also used by the virus to enter CD4+ T cells and subsequently replicate. Objective: To screen several proteins in Indonesian plants for the potential to bind to the CD4 receptor. Method: Molecular docking was carried out on Cluspro 2.0 specifically for protein to protein and MOE for protein to ligand. Results: The protein ligands were Cinnamomin III, Agglutinin, PAP, PAP-S, Momordin I, MAP30, Beta-luffin, Luffaculin I, Cucurmosin, DAP, Dianthin-30, Bouganin, Maize, Ricin, Abrin, Balsamin, and the bond energy values (Joules/kg.mol) were -602.8, -973.5, -511.3, -439.1, -532.2, -661.9, -487.0, -472.8, -530.9, -413.6, 444.1, -504.5, -617.2, -855.6, -883.9, -558.6, respectively. The best binding energy with CD4 was selected to identify the compound's molecule. These compounds were abrusin, abrusogenin, eicosadienoic acid, heneicosane, precatorine, and trigonelline, and the bond energy values (Joules/kg.mol) were -19.2158, -16.7057, -15.5155, -13.9632, -15.6119, and -9.2620, respectively. The toxicity test of abrusin was carried out against 18 targets, and two targets showed toxic activities. Conclusion: The content of RIP and natural chemical compounds in Abrus precatorius seeds make them the best candidate for antiretroviral therapy against HIV-1.

SYSTEMATIC REVIEW: POTENSI ANTI-INFLAMASI PADA TANAMAN PETAI (Parkia speciosa)

Anti-inflammatory is defined as a compound or class of drugs that has the activity of suppressing or reducing the inflammatory response. Inflammation is a dynamic body defense mechanism and can be acute or chronic. There are still many Indonesian people who self-medicate (self-medication) using modern and herbal medicine. One of the native Indonesian plants used as a herbal medicinal plant is petai (Parkia speciosa). One of the known benefits of petai is as an anti-inflammatory because of the flavonoid content in it. The aim of this research is to examine the potential anti-inflammatory activity of the petai plant (Parkia speciosa). This research method was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The articles used in this research came from databases such as Google Scholar, Pubmed, Semantic Scholar, Researchgate, Ebsco with the keywords used namely (Parkia speciosa) AND (stink bean) AND (anti-inflammatory). There were 8 articles taken for review. The conclusion is that petai (Parkia speciosa) seed, skin and leaf extracts have anti-inflammatory potential based on in vitro and in vivo tests.

Toxicity Evaluation with Brine Shrimp Lethality Test and Phytochemical Analysis of Some Indonesian Plant Extracts As Potential Anti-Colon Cancer Agents

Toxicity Evaluation with Brine Shrimp Lethality Test and Phytochemical Analysis of Some Indonesian Plant Extracts As Potential Anti-Colon Cancer Agents

Pigment Extraction Method for Anthocyanin Natural Resources in Indonesia: A Review

Anthocyanin is the pigment that still attracts attention from food industries because of its abundance in Indonesian plants. Anthocyanins are produced in a variety of colors with unique stability properties. Through a systematic review, this review studied 40 scientific reports conducted in Indonesia over the last 10 years. The research objects included fruits, flowers, seeds, peels, and roots from the islands of Java, Sumatra, Kalimantan, Sulawesi, and Nusa Tenggara. Several methods are used to extract solvent-based anthocyanin compounds, including maceration, microwave, and reflux-soxhlet extraction. Microwave extraction, including ultrasonic extraction, is recommended for anthocyanin extraction. Spectrophotometry with the pH differential method was chosen as the anthocyanin quantification procedure, using 500 and 700 nm wavelenghts.

Biodiversity of Banana Trees (Musa sp.) in Purwodadi Botanical Gardens

Banana is a fruit that is often found in everyday life. This fruit is a daily consumption to meet daily nutritional needs. Indonesia has approximately 200 types of bananas scattered throughout the island. Diversity of banana trees (Musa sp.) in an effort to conserve Indonesian plants that live in lowland forests by carrying out plant exploration and collection activities. This research was conducted at the Purwodadi Botanical Gardens (KRP) with the aim of knowing the collection of banana trees (Musa sp.) in KRP. The method used is the roaming method with observation data collection. The results showed that there were 44 species of banana trees (Musa sp.) found alive, including the wild species with seeds Musa acuminate Colla and Musa balbisiana Colla. The species found consisted of 6 kinds of cultivar genome groups that had different characteristics. Banana tree cultivation was developed because it has many benefits and nutritional content for health.

Karakteristik Kimia dan Organoleptik Nugget Ikan Tuna (Thunnus sp.) dengan Penambahan Pasta Kenari (Canarium indicum L.)

Fish nuggets are one of the diversified fishery products. Groundfish meat is added with binding ingredients and seasonings, then steamed and molded. Tuna (Thunnus sp.) is a fish with high economic value and is relatively abundant in Indonesian seas. Walnuts (Canarium indicum L.) are one of the native Indonesian plants which are widely used as food. This study aimed to determine tuna nuggets' chemical and organoleptic characteristics by adding walnut paste. This study used a one-factor complete randomized design: walnut paste concentration with four treatment levels of 0%, 25%, 50%, and 75% with tri repeats. Observed variables include water, ash, fat, protein, carbohydrate content, hedonic tests, and hedonic quality of color, taste, aroma, and texture. The results showed that giving walnut paste affected fish nuggets' chemical and organoleptic properties. The water content of fish nuggets decreases, while the ash, fat, protein, and carbohydrate content increases with the addition of walnut paste concentration from the beginning of treatment, influencing the hedonic assessment and hedonic quality.

Variation of Phenology of Flacourtia rukam in Two Different Habitats and Their Relation to Rainfall, Dry Days, and the Plant Water Status

Phenological shifts in the emergence of vegetative and generative parts, occur due to the response of plants to water received due to climate change. The rukam tree (Flacourtia rukam (Zoll & Moritzi)) is a local Indonesian plant with potential as an edible fruit and antioxidant agent, but its existence is increasingly rare. F. rukam can be found in Pasuruan, East Java, including Purwodadi Botanical Garden (PBG) as collected plants and Sekarmojo Village as cultivated plants. These populations show a shift in phenology, but this has never been studied. This study aimed to analyze the spatial variation of the phenology of two rukam populations and its relation to plant water status, rainfall quantity (RQ), and drought days (DD). This research was conducted on two individual trees at each location, was conducted from August 2020 to October 2022 using secondary data from BMKG, such as daily rainfall and the number of days without rain. Phenology was directly observed every week with an estimated abundance of numbers 1-4; plant water status was observed by calculating relative water content (RWC); soil physical analysis was carried out in mid-season. Data were analyzed by Mann-Whitney test and SEM WarpPLS. The results showed differences in phenology in the phases of leaf fall, flower buds, young fruit, and ripe fruit on the trees in the different locations. The decreased RQ in the past month significantly reduced the RWC value of F. rukam in both places, while the number of DD contributed to a decrease in the RWC value in PBG only, not in Sekarmojo. In both sites, decreasing the RWC of the rukam increased the abundance of fallen leaves and the chances of flowering. Rainfall and drought days affected plant phenology directly or indirectly through RWC mediation. In both places, RQ had a direct positive and significant effect on blooming flowers and new leaves but had negative effect on fallen leaves. While DD had a positive effect on leaf fall, flower formation, and fruit ripening, but had a negative effect on the appearance of young fruit (in PBG). Indirectly, RQ mainly affected several phenological parameters, while DD did not affect all phenological parameters.

PTP1B Inhibitors from Indonesian Plants and Its Structure-Activity Relationship: A Review

PTP1B Inhibitors from Indonesian Plants and Its Structure-Activity Relationship: A Review

Total Flavonoid Content and In Vitro Anti-Inflammatory Potentials of Kombucha with Enrichment of Butterfly Pea (Clitoria ternatea) Flower Extract

Kombucha is a fermented beverage from tea (Camellia sinensis) that is claimed to be beneficial for several medical ailments. Several studies have conducted nutritional enrichment of kombucha to optimize its nutritional benefits. One of the native Indonesian plants that can be utilized as nutritional enrichment material is the butterfly pea (Clitoria ternatea) flower. Its nutrition is expected to increase the health benefits of kombucha Camellia sinensis. Kombucha has beneficial compounds such as flavonoids that have potential applications in medical chemistry due to its anti-inflammatory properties. This study aims to enrich the valuable nutrition and test the in vitro anti-inflammatory potentials of kombucha, conducted by formulating kombuchaCamellia sinensis with Clitoria ternatea flower extract addition (0; 0.5; 1; 1.5; 2)% (m/v), the chemical characterization including pH value test using digital pH meter and total flavonoid content (TFC) using colorimetric (AlCl3) assay) with quercetin as its standard, and in vitro anti-inflammatory activity test using the red blood cell (RBC) membrane stabilization method. The result showed that the pH value of kombucha had met the consumption quality standard (3-4), starting from 3,30 + 0,00 (0%) to 3,66 + 0,01 (2%). The flavonoid content increased from 38,89 + 0,05 mgQE/g (0%) to 126,73 + 0,13 mgQE/g (2%). Kombucha samples showed anti-inflammatory activity potentials with the best IC50 value of 140,22 ppm (2% addition). The data from this study were analyzed using a one-way ANOVA statistical test. It shows there is an influence from the addition of Clitoria ternatea to the TFC result (p<0,05), and it continued to Duncan's Multiple Range test (DMRT) that showed sample F0 to F4 were significantly different from each other (p<0,05). In conclusion, the enrichment of kombucha (Camellia sinensis) using Clitoria ternatea flower extract has been proven to increase the health-beneficial compound (flavonoids) and have potential anti-inflammatory activity.

Volatile Compounds Content and Sensory Profile of Katuk (Sauropus androgynus) Leaves After Household Scale Heating

Katuk (Sauropus androgynus) is an indigenous Indonesian plant, and the leaves are often consumed as fresh or processed vegetables. Despite its potential, there are no reports on the volatile compounds content and sensory profile of Katuk leaves after being processed using household scale heating. Therefore, this study aimed to determine the volatile compounds content and sensory profiles of Katuk leaves after household scale heating (steaming and boiling). The extraction of constituents was carried out using the headspace-solid phase microextraction method, followed by identification with gas chromatography-mass spectroscopy. The sensory profile analysis was performed using the free choice profiling method with untrained panelists. The analysis results showed the presence of 16 volatile compounds derived from 7 groups, including aldehydes (5 compounds), alcohol (3 compounds), other components (3 compounds), ketones (2 compounds), as well as benzene, terpenoids, and esters (1 compound). The dominant compounds found in fresh Katuk leaves were alcohol [(Z)-3-hexene-1-ol]. Meanwhile, aldehydes (benzaldehyde, nonanal, benzeneacetaldehyde, and (E)-2-pentenal) and alcohol (1-Heptanol and Nerolidol) were dominant in the steamed samples, with benzene (naphthalene) being predominantly found in the boiled samples. The sensory profile analysis results showed that fresh Katuk leaves had a grassy and earthy aroma with a grassy flavor, while the boiled samples had a smooth and juicy texture. The dominant attributes found in steamed Katuk leaves included moist, tender, and tasteless. Based on these findings, household scale heating could modify the composition of volatile compounds, thereby affecting the sensory profile. The results obtained were expected to serve as a foundation for the processing of Katuk leaves at both industry and household levels.

Potential of Indonesian Plants as Polymicrobial Anti-Biofilm

Biofilm infection occurs in 80% of chronic infections caused by 60% of biofilms from plankton cells and polymicrobial biofilms. Due to synergistic interactions between species, infections caused by polymicrobial biofilms are more virulent than monospecies biofilm infections. New anti-biofilm candidates are constantly being developed by tracing the content of active compounds from medicinal plants native to Indonesia. The need to find new plant sources that have the potential as anti-biofilms is increasingly needed along with increasing microbial resistance. Various studies show that active compounds that have anti-biofilm potential are polyphenols, quercetin, curcumin, gallic acid, and ferulic acid. The mechanism of action of anti-biofilms is through the prevention of attachment and formation of biofilms, inhibition of quorum sensing, and inhibition of gene expression in microbes.

ISOLASI ALFA SELULOSA DAN PEMBUATAN SERTA KARAKTERISASI MIKROKRISTALIN SELULOSA DARI KULIT BUAH LIMPASU (Baccaurea lanceolata Muell)

Indonesia is rich in biological natural resources and has diversity spread throughout Indonesia. One of the typical Indonesian plants, especially in Kalimantan, is Limpasu. Limpasu is a typical Kalimantan plant, where this plant has many nutritious contents such as antioxidants and has high potential to be used as a raw material for making microcrystalline cellulose. Microcrystalline Cellulose (MCC) is cellulose that is partially purified, then processed with ɑ-cellulose preparations. The aim of this research is to obtain microcrystalline cellulose from Limpasu fruit peel waste. The research method used in this research is chemical delignification, followed by hydrolysis using dilute acid to produce MCC, then characterized with Avicel PH 102 as a commercial comparison. Based on the research results, the yield of α-cellulose was 14.14% for simplicia powder, the MCC yield was 11.48% for α-cellulose and the characterization of MCCKBL powder showed similar characteristics to Avicel® PH 102 as a comparison.

The Growth Response of Rendeu (<i>Staurogyne elongata</i> (Neese) Kuntze) to Shoot Pruning and Its Propagation by Shoot Cutting

Rendeu (Staurogyne elongata (Neese) Kuntze) is a native Indonesian plant used as food and traditional medicine in the daily life of the people residing around Gunung Halimun-Salak National Park. Due to the potential source of herbal-based medicines and traditional food in the long-run purposes, the proper method of its propagation is required, so that Rendeu can be conserved and utilised sustainably. This study employed two research designs. First, a completely randomized design with pruning and IAA (indole-3 acetic acid) treatment was used for seedling growths. Second, plant propagation applied a factorial randomized block design: planting media types and plant growth regulator (PGR) (rootone F) treatment. Observation included the number of buds, number of leaves, number of flowers, plant biomass, root length, and relative chlorophyll content using the SPAD tool. The data were analysed using ANOVA (SPSS ver. 17.0), followed by Pearson correlation analysis. The results showed that applying IAA and leaf pruning could increase the number of buds, the number of leaves and the fresh weight of S. elongata plants compared to the control plant. The addition of rootone increased the growth of Rendeu shoot cuttings, shown in all growth parameters and chlorophyll content. Humus was the best media for Rendeu’s growth among all planting medium. Planting media affected the increase in the number of leaves and the number of buds of S. elongata significantly. The interaction of planting media and PGR somewhat influenced root length and total leaf chlorophyll. The growth and production of S. elongata increased with the time of planting.