Indonesian Coffee And Cocoa Research Institute Research Articles

Growth of cocoa seedlings affected by application of zeolite-cocopeat-manure mixture as soil conditioner enriched by Trichoderma sp.

The objective of this research was to study the effect of soil conditioner in different dosage levels enriched by Trichoderma sp. on cocoa seedling growth and biomass. This research was conducted in glass house Kaliwining,Experimental Station of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, East Java, with rainfall type of D (Schmidt & Ferguson) with relatively flat topography at altitude 45 m above sea level. Complete Randomized Design using four treatments, i. e. no application of soil conditioner (control), the application of 200 grams, 400 grams, and 600 grams/polybags of soil conditioner, with 5 replications and 5 subsamples was engaged for this research. Trichoderma sp. as endophytic fungus has been detected highest in the soil conditioner that enriched with Trichoderma sp. The result of the research was the application of soil conditioner alone or combined with Trichoderma sp. could increase plant growth and plant biomass. This research finding was the addition of Trichoderma sp. with soil conditioner increased the plant growth doubled than soil conditioner alone.

Growth of Two Cocoa (Theobroma cacao L.) Planting Materials on Three Growing Media Composition

Seed growth and uniformity are keys in producing high quality and yield of cocoa (Theobroma cacao L.). Plant growth is influenced by internal factors (genetics)and external factors, including planting media. This study aimed to determine the effect of cocoa planting material types and planting media of cocoa seedlings.The research was conducted at Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia, using a split-plot group randomized design. The main plot was the planting media consisting of topsoil, topsoil and rice husk, and topsoil and manure. The subplots consisted of two types of cocoa planting material: hybrid seeds ICCRI 08H and half-sib Sulawesi 1. Each treatment was replicated three times. The results showed that the media influenced on seedling growth uniformity, plant height, stem diameter, and number of leaves. Genetic factors affect plant height and stem diameter. The interaction of media and the type of planting material affect the stem diameter and number of leaves. Cocoa seedling growth with the best plant height and stem diameter was shown in topsoil and manure media, while the response to the number of lleaves showed differences between ages and planting media used. ICCRI 08H hybrid cocoa planting material showed the highest plant height and stem diameter compared to Sulawesi 1 half-sib

Morphological and Molecular Identification of Helopeltis Species on Cocoa from Kaliwining Experimental Station, Jember, Indonesia

Severe crop loss of cocoa in Indonesia has long been reported to be caused by Helopeltis. It has been noted that cocoa and Helopeltis have existed in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia, for about 100 years, and the species have developed during that time. Our study aimed for examining the Helopeltis species that attacked cocoa in this experimental station both morphologically, morphometrically, and molecularly and to compare the species with the one attacking cocoa in Java and Nusa Tenggara, preserved at the GenBank. Helopeltis was sampled from infested cocoa pods collected representatively from cocoa at the Kaliwining ES and then reared in the Crop Protection Laboratory of ICCRI. Adults of Helopeltis laboratory-reared insects were identified, 30 females 30 males were selected. Weexamined the morphology and morphometry of the samples, and their identifica tion was based on an insect identification key. Morphometry measurement of male and female body parts includes body length, antenna segments, head, eyes, collar, wing, tibia, and femur. In addition, the molecular identification of Helopeltissp. was made through DNA extraction, amplification, and sequence analysis of DNA target regions using the COI part of mitochondrial DNA. External morphological identification, supported by morphometry of the Helopeltis specimen from Kaliwning, the white bands on all femora, body length, and the ratio of the firstantennal segment to the posterior width of the pronotum suggested that the insect belongs to Helopeltis bradyi. Similarly, molecular identification using PCR amplification through specific primers from COI gene sequences confirmed that Helopeltis specimens from Kaliwining are classified as Helopeltis bradyi.

Germination and Early Seedling Growth of Three Arabica Coffee Varieties at Four Seed Soaking Water Temperatures

It takes three weeks to analyze the germination percentage of Arabica coffee seeds germinated using paper and a three-day water-soaking method. This 3- week period is considered too long for seed distribution to remote areas. Thus, we need to modify this germination test needs to be modified to have a shorter test time. Temperature treatment is one method to break the seed dormancy period for faster germination. This study aimed to obtain a faster and more precise method of germination test on Arabica coffee seeds. This study employed a 3 x 4 factorial complete randomized block design with three replications in which Arabica coffee varieties of Gayo-1, Kartika-1, and Sigararutang as the first factor and the temperature of the soaking water of the seeds (25 0C, 50 0C, 75 0C, and 90 0C) as the second factor. The research was conducted at the Plant Breeding Laboratory, Indonesian Coffee and Cocoa Research Institute (ICCRI), for four months. Findings showed that the interaction between treatments was only found in seed germination percentage. Soaking in room temperature water (control) for three days was better than using water at 50 0C, 75 0C, and 90 0C on the germination percentage, sprout length, and vigor index. Observations of Arabica seed germination could be shortened to 14 days compared to the standard 21-day observation. During the seedling phase, the shoot growth and root growth of the Arabica coffee seeds soaked in water at 50 0C showed no significant difference from the control. Soaking at 75 0C and 90 0C damaged the embryo and resulted in rotten seeds. Different varieties led to different germination percentages, sprout lengths, and fresh weight of seedling shoots. The method of soaking at 50 0C for 30 minutes could be used as an alternative in germination test of Arabica coffee seeds because it showed small negative effect on germinated seed and seedling quality but it shorten thetesting time for three days.

DNA Barcoding for Identification of Helopeltis sp. from Kaliwining Experimental Station using Mitochondrial COI Gene


 
 
 Helopeltis sp. is one of the major pests of the cocoa plant. Helopeltis can decrease cocoa yield by 50 to 60%. Several species of Helopeltis spp. are still often misidentified due to similarity in morphology such as size and color. This study aimed to identify Helopeltis sp. through DNA barcoding using the mitochondrial cyto- chrome C oxidase subunit 1 (COI) gene. Analysis was carried out on 100 adults Helopeltis sp. from Kaliwining Experimental Station, Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia. Specimens were preserved in 96% ethyl alcohol at 4oC until molecular studies were carried out. PCR amplification was conducted through specific primer from COI gene. Sequences data analysis using MEGA 5.05 software was carried out. The study results using DNA barcoding showed that Helopeltis samples in Kaliwining cocoa plantations are Helopeltis bradyi with a percentage identity above 99% and E value of 0; this sequence as significantly matched. Mean length of the sequences was 679.5 base pairs. Phy- logenetic tree data also shows that the Helopeltis species analysed were also of the same descent, H. bradyi with the accession number KT189562.1. Helopeltis sp. specimens were clustered together. No taxonomic deviation was detected at the species level, indicating that most of the examined species of Helopeltis sp. could be authenticated by the barcode approach using the COI gene. In addi- tion, Helopeltis specimens were also aligned with H. antonii and H. theivora from the genebank to determine their genetic relationship.
 
 

Clonal differences in nitrogen use efficiency and macro-nutrient uptake in young clonal cocoa (Theobroma cacao L.) seedlings from Indonesia

Nitrogen (N) is one of the most required nutrients in cocoa production. Unlike in many other crops, there is limited knowledge on genotypic variation in nitrogen use efficiency (NUE) in clonal cocoa. We therefore evaluated the effect of nitrogen application on cocoa NUE in 10 clonal cocoa scions that were top-grafted on 4-month old rootstock seedlings of cocoa clone MCC 02. Grafted seedlings received either 0 or 2.3 g/plant of N at the beginning of the experiment, which lasted for five months and was arranged in a nursery of the Indonesian Coffee and Cocoa Research Institute (ICCRI) in Jember, Indonesia. A factorial randomized block design was used with four replications. We found that clones significantly affected rootstock stem diameter and dry biomass both in roots and shoots. Nitrogen application decreased rootstock stem diameter and root biomass but increased shoot-root ratio. Following the N application, we observed significantly higher N concentration and uptake both in roots and shoots. This higher N concentration and uptake contributed to significantly lower NUE in both roots and shoots. We did not observe clonal differences in N concentration, uptake, NUE in either roots or shoots and ANR (Apparent Nitrogen Recovery). Nitrogen application influenced P, K, Mg, and Ca uptake in both roots and shoots. There was a clonal effect on P, K and Mg uptake and on P and Mg use efficiency in both roots and shoots. This implies that the studied clones vary in their P and Mg nutrient use efficiency but not in NUE.

Sensory profiles of cocoa genotypes in Indonesia

Abstract. Sari IA, Murti RH, Misnawi, Putra ETS, Susilo AW. 2021. Sensory profiles of cocoa genotypes in Indonesia. Biodiversitas 23: 648-654. The Cocoa flavor is influenced by genetic factors and has an important role in increasing the added value of beans as specialty products. For this reason, superior genotypes with specific and unique flavors should be identified as fine flavor cocoa (FFC) products. This study was conducted to obtain genotypes with sensory quality and specific profiles. Ten clones under three groups were used: Forastero (KW 516 and KEE 2), Trinitario with purple beans (ICCRI 03, ICCRI 07, ICCRI 09, MCC 02, Sulawesi 1, Sulawesi 2, and TSH 858), and Trinitario with white beans (DR 2). Genotypes were assigned using a completely randomized block design with three replications. A sensory test was performed by three trained and certified panelists using the Indonesian Coffee and Cocoa Research Institute (ICCRI) and Guittard Chocolate Company standards. The attributes, acidity, bitterness, astringency, fresh fruit, browned fruit, floral aroma, woody aroma, spicy, nutty taste, and browned/roasted appearance of cocoa were evaluated on a scale of 1-10. Data were examined through ANOVA, flavor character profiling, PCA Biplot, and dendrogram analysis in STAR 2.0.1, Microsoft Excel 2019, and R Studio. Results showed that the genotypes significantly influenced the chemical content of the beans, and each genotype had a specific flavor character. The genotypes were classified into three groups based on flavor characters via PCA biplot and dendrogram analysis. Two groups of aromatic genotypes had the potential to develop FFC products with weak (DR 2) and strong cocoa taste (ICCRI 09, ICCRI 03, ICCRI 07, MCC 02, and TSH 858). Conversely, the nonaromatic cocoa groups with strong taste were KEE 02, KW 516, Sulawesi 1, and Sulawesi 2. Therefore, the intensity of floral and nutty aromas significantly affected the overall quality of cocoa flavor.

Growth Response of Robusta Coffee (Coffea canephora L.) Seedlings to Exogenous Salicylic Acid Application

To accelerate growth performance of Robusta coffee (Coffea canephora) seedling could be conducted through the application of exogenous plant hormone.Salicylic acid is one of plant hormones that widely applied to increase the plant growth for several plants, however, for coffee was still relative limited. This studywas aimed to determine the effect of salicylic acid on the growth of Robusta coffee seedlings which research was conducted at Indonesian Coffee and CocoaResearch Institute (ICCRI), Jember, East Java. This experiment was set using split plot design with three replications. The main plot was clones, which were BP 308and Sintaro clones. The seedlings were propagated from rooted cuttings. The subplot was the concentration of exogenous salicylic acid, that were control,0.6 mM, 1.2 mM, and 1.8 mM. The result demonstrated that BP 308 clone had higher rooting performance than Sintaro clone that shown by the higher rootvolume, root dry mass, and root/shoot ratio. In general, the application of salicylic acid could enhance the growth of Robusta coffee seedling. The application of 0.6 mMsalicylic acid increased plant growth of coffee seedlings. There was different response to concentration of application salicylic acid for both clones. The application of 0.6 mM salicylic acid could increase plant growth of BP 308, includeplant height, leaf area, stem dry mass, leaf dry mass, root/shoot ratio and total dry mass. Meanwhile, on Sintaro clone, application of 1.2 mM salicylic acid couldincrease leaf area, stem, leaf and total dry mass. The application of salicylic acid more than 1.2 mM led to detrimental effect of coffee seedlings.

Diversification strategy in Dealing the Development of the world coffee products (Study On Coffee And Cacao Research Centers Indonesia/ICCRI )

Indonesian Coffee and Cocoa Research Institute (ICCRI) is a institution that Conducting research to obtain technological innovation in the field of cultivation and processing of coffee and cocoa products. ICCRI has an Experimental Garden and Office Area of 380 ha, consisting of arabica coffee experimental garden (KP Andungsari height of 100-1,200 m asl), robusta coffee and cocoa (KP Kaliwining and KP Sumberasin 45-550 m above sea level). The laboratory has an area of 2,365 m2 with equipment of 850 units. Consisting of Plant Breeding Laboratory, Soil Physics Laboratory, Soil Chemistry and Soil Biology, Tissue Culture Laboratory, Agricultural Mechanization Laboratory, Product Processing Laboratory, Quality Control Laboratory, Information Center and Training. The purpose of this research is to provide an overview of diversification by Indonesian Coffee and Cocoa Research Institute (ICCRI) to further develop coffee production business in Indonesia. Analysis data used in this study is discriptive analysis to describe holisticly and also SWOT analysis to discribe deeply strength weakness opportunity and threat to make strategy to improve the performance. The result of the research show that needed a diversification business conducted in the form of internal deverifikation by doing research to get a better quality coffee plant, quality and have high competitiveness at national and international level. The resulting coffee plant is also resistant to plant diseases, more fruiting, better fruit quality. Deverification is also carried out by performing aoutonomous fund with the establishment of Coffe and Cocoa science techno park which is also intended to disseminate the results and activities of ICCRI to the community.

Cocoa Production as Affected by Shade Tree Species and Soil Quality

Cocoa is one of the agricultural commodities that have important role and are reliable in realizing agricultural development program of Indonesia. The cocoaproduction of Indonesia tends to decrease that caused by in appropriate farming. This research aimed to evaluate the shade management to cocoa productivity and evaluating the correlation between soil quality and the cocoa production with the different shade management. This research was conducted from October 2016 until June 2017 in Kaliwining Experimental Station and Soil Laboratory of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia. Clones at the trial plots were Sulawesi 1 and Sulawesi 2, 10 years old, and arranged in 3 m x 3 m spacing. The design of this research was conducted by observing parameters of sample trees on cocoa field. This research used three observation plots, with shade trees of leucaena (Leucaena sp.), teak (Tectona grandis), and teak combined with krete (Cassia surithensis) shade. The results indicated that shade species affected the cocoa production. Teak shade gave higher production of cocoa (i.e 3,01 kg.tree-1) compared to other species of shade (1.83 and 1.39 kg.tree-1 for leucaena and teak and krete combination, respectively). The correlation of soil quality with cocoa production showed that soil calcium content, earthworm biomass and soil bulk density were positively correlated with the production of cocoa. Shade species greatly affected the production in combination with the soil quality.

Morphological variation among fifteen superior robusta coffee clones in Lampung Province, Indonesia

Ramadiana S, Hapsoro D, Yusnita Y. 2018. Morphological variation among fifteen superior robusta coffee clones inLampung Province, Indonesia. Biodiversitas 19: 1475-1481. This study aimed to characterize morphological variation among fifteensuperior robusta coffee clones in Lampung Province. The fifteen clones consisted of four clones released by the Indonesian Coffee andCocoa Research Institute (ICCRI), i.e. ‘BP 409’,‘BP936’, ‘BP939’, ‘SA 237’, together with eleven superior coffee clones selected byfarmers from Tanggamus District (‘Tugino’, ‘Wanto’, ‘Biyadi’, ‘Komari’, ‘Wardi’, ‘Wariso’) and from West Lampung District (‘TuguKuning’, ‘Tugu Hijau', ‘Tugu Biru', ‘Tugu Sari', ‘Lengkong'). Fifteen qualitative and seven quantitative morphological characters wereevaluated in a randomized complete block design with three replicates for each clone,each replication consisted of two plants. Fromeach plant, four samples were taken from four sides of the plant (north, south, west, and east). It was found that while somemorphological characters exhibited negligible variation among clones, there were significant differences between clones for othercharacters. The characters that did not differ significantly between clones were: shapes of leaf base and leaf tip; petiole color; leafvenation pattern; fruit disk shape; ripe-fruit color; and stipule shape. The characters that varied between clones were shape of leaf lamina(elliptical vs. lanceolate); fruit shape (round vs. oval); and shape of leaf margins. Morphological variation was also observed in somequantitative characters: canopy diameter; tree height; stem diameter; leaf length; leaf width; petiole length; stipule length; and number ofprimary branches.

Heterosis Analysis of Leaf Stomatal Characteristics on F 1 Population of Cocoa (Theobroma cacao L.) Related to Vascular-Streak Dieback Resistance

Breeding for cocoa resistance to VSD is the main goal in breeding program in Indonesia. Research for selection criteria on cocoa (Theobroma cacaoL.) resistance to vascular-streak dieback (VSD) has being carried out by exploring leaf stomatal characteristics corresponding to VSD damages. This research had objectives to select best crosses by observing the estimated value of heterosis and heterobeltiosis on F1 hybrid populations based on leaf stomata characteristics related to VSD resistance. Trial was established at Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute (ICCRI) in a VSD endemic area. Treatments consisted of 5 F1 hybrid populations generated by biparental crosses of TSH 858(S) x Sulawesi 1 (R), TSH 858 (S) x KEE 2 (R), TSH 858 (S) x Sulawesi 3 (R), Sca 6 (R) x KW 264 (S) and KW 617 (R) x KW 264 (S). Each cross consisted of 100-300 genotypes planted in high planting density of 0.5 m x 0.5 m. Theresistance was evaluated by scoring the plant damage in the scale of 0-3 at three years after planting. Stomata characteristics were assessed microscopically based on the variables of density, length, width and diameter of stomata and width of opening stomata. Analysis of correlation indicated that most of stomatal charac-teristicssignificantly correlated to VSD damage but the width of opening stomata performedthe highest coefficient of correlation (r = 0.49) and contributed to VSD damage (0.45). Meanwhile, path analysis indicated higher value of the char-acteristics performed less resistance of the tested hybrids, KW 617 x KW 264 showed lower value of heterosis and heterobeltiosis for the characteristics which of the best crosses for generating VSD-resistant genotype.

Stomatal, Proline, and Leaf Water Status Characters of Some Cocoa Clones (Theobroma cacao L.) on Prolonged Dry Season

ENSO (El Nino Southern Oscillation) occurring in 2015 in Indonesia caused drought stress and the decrease in the percentage of production and even death of plants. One effective and relatively inexpensive way of reducing damage and economic losses due to drought is the availibility of tolerant cocoa. The objective of this research was to obtain tolerant clones based on morpho-physiological characters under drought stress. The experiment was conducted in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute (ICCRI) during dry season (El Nino period with 5 consecutive dry months, from May to October 2015). Stomata, proline, leaf water status was measured at the peak of dry season on tested cocoa clones. The cocoa clones that predicted tolerant against to drought stress were KW 641, KW 514, KW 535, KW 619, and KW 516, whereas the cocoa clones that predicted susceptible under drought stress were KW 609, KW 614, KW 635, KW 606, and KW 651. The treatment clones had higher values of RWC, leaf proline content, narrower width and length of stomata openings, but smaller value of WSD compared to susceptible clones. Meanwhile, the character of leaf thickness might not significanly different to the character of drought tolerance.

Development of Pratylenchus coffeae in Biochar Applied Soil, Coffee Roots and Its Effect on Plant Growth

Biochar improve physical, chemical and biological properties of soil. It may also be used as botanical pesticides. The experiment was aimed to determine the effect of biochar on population development of parasitic nematode Pratylenchus coffeae. The experiment was carried out in Nematology Laboratory and in a greenhouse of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia. The experiment was arranged according to completely randomized design with six treatments of biochar concentrations, i.e. 4%; 3%; 2%; 1%; 0.5% and 0% dry weight of soil. Research results revealed that biochar application of 4.0% was effectively killed 74.5% of P. coffeae, while the lowest mortality level of 37.5% at biochar 0.5% treatment. In the greenhouse trial, application of biochar 4.0% was the most effective in suppressing the population of parasitic nematode in coffee seedlings. Application of biochar was also able to increase the growth and biomass of coffee seedlings.

Application of Copper Fungicide and Row Covers to Control Phytophthora Seedling Blight Disease on Cocoa

Phytophthora seedling blight disease is one of the important diseases in cocoa. The disease is caused by the fungus of Phytophthora palmivora belongs to class Oomycetes. The aim of this research was to determine the effectiveness of the use of row covers and copper oxide fungicide applications to control seedling blight Phytophthora. Covering treatment was to place cocoa seedlings inside bamboo frame covered by transparent plastic in order to avoid from rainfall effect. The research was conducted in the Kaliwining Experimental Station at Indonesian Coffee and Cocoa Research Institute (ICCRI). Experiments were carried out four treatments and repeated 6 times. Each treatment consists of 100 seedlings planted in polybags and placed in the nursery area with a protective paranet. The treatment consisted of (1) row covers, (2) spraying of copper oxide 0.2%, (3) a combination of row covers and copper oxide 0.2% and (4) control (no covers and spray). Observations made 7 days after treatment with an interval of 7 days for 10 weeks. The results showed that row covers able to prolong the incubation period of the disease for 14 days, while the application of row covers + copper oxide 0.2% was able to save cocoa seedlings by 96.2%.

Abundance of Pollinator Insect (Forcipomyia spp .) of Cocoa Under Some Shade Trees

Cocoa production is affected by cocoa flowering and fruiting. The factor affects fruit seeting is pollinator agent such as Forcipomyia spp. Until now, information concerning population dynamics of Forcipomyia in some models of cocoa shading trees remains limited. This research was studied to observe the population dynamics of Forcipomyia spp. in some models of cocoa shading trees, namely lamtoro (Leucaena sp.), krete (Cassia surithensis) and areca nut (Areca catechu) in two main season of rainy and dry seasons. The research was conducted in Kaliwining research station of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, by surveying the existing cocoa under different types of shading trees as mentioned above. The insects were observed using modified sticky trap method, whilst micro climate condition was also observed. The highest population was in January (rainy season) under Leucaena spp. shade tress and the lowest population was in October under all type of the shading trees. There was no relationship between microclimate condition under cocoa trees (temperature, RH and light intensity) and Forcipomyia spp. population (r = 0.08 and 0.04).

Relationship Analysis Between Leaf-Stomata Characteristics with Cocoa Resistance to Vascular-Streak Dieback

Characteristics of leaf-stomata indicate having relationship with the resistance of cocoa to vascular-streak dieback (VSD) caused by Ceratobasidum theobromae. This research has objective to identify the relationship between leaf-stomata haracteristicsto VSD resistance in order to develop criteria for selection. Trial was establised in randomized-complete block design with three blocks as replications in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute (ICCRI) using 15 clones which had different background on VSD resistance. Leaf-stomata of the tested clones were characterized using variables of number of stomata, stomata size, width of opened-stomata and size of stomata mouth in three different level of leaf growth, namely flush, young and mature. VSD resistance was assessed by scoring the damage in the scale of 0-6 that the tested clones were classified into five group sof resistance. Analysis of variance indicated a significant effect of cocoa clone to the number of stomata in all categories of leaf sample but the other leaf-stomata haracteristics were significantly affected by cocoa clone just at the young and mature leaf. Correlation analysis performed a significant inter-correlation between variables of leaf-stomata characteristics in young leaf and the score of VSD resistance. However, number of stomata and width of opened-stomata in the mature leaf were significantly correlated to the score of VSD resistance. Genetic variance analysis showed a high category of broad sense heritability for the number of stomata and width of the penedstomata in young leaf that both of the variables can be developed as criteria for selection on VSD resistance.

An Alternative Model of Cocoa Production Institution: A Solution in Facing Asean Economic Community

There is an indication that cocoa production institution is not working well as farmers’ expectations. The lack of coordination among institutions cause cocoa institutions are not institutionalized. The study are expected to: (1) construct the institutional model for enhancing competitiveness of cocoa exports in facing Asean Economic Community, (2) formulate a coordination model among institutions of cocoa production in accelerating Indonesian economic development. ISM analysis results show that: first, there are 11 institutions that should be more pro-active in improving cocoa production, namely: (1) Indonesian Coffee and Cocoa Research Institute (ICCRI), (2) Gardens Management Coordination Forum (FKMK), (3) Indonesian Cocoa Association (ASKINDO), (4) Indonesian Cocoa Farmers Association (APKI), (5) Indonesian Cocoa Industry Association (AIKI), (6) Provincial Office for Plantation, (7) Financial Institutions/Banks, (8) Marketing Agencies, (9) Local Office for Forestry and Plantation, (10) Extension Service Officer in District level, and (11) Joined Farmers Group (Gapoktan). Second, through the formulation of structural model of coordination in the institutional production of cocoa in the acceleration of economic development, it was revealed that the main causal of the coordination function weakness are (1) the weak of institutional training, and (2) the impact of regional autonomy policy, implies that an alternative model of cocoa production should focus on improving institutional training and reducing negative impact of regional autonomy.

Performance of Cooled Cone Grinding Machine in Cocoa Cake Processing

The process of cocoa paste pressing has a function to separate the fatty component of cocoa from its cake. Cocoa paste is further processed into cocoa powder using grinding machine for cocoa cake. The cooled cone type of cocoa grinding machine is used to solve the problem of plug in the maschine caused by melting of fat in cocoa cake due to hot effect as a result of friction in the grinding machine. Grinding machine of cocoa has conical form of cylinder for grinding and stator wall wrapped by source of cold and closed with jacket wool. Research was conducted at Kaliwining Experimental Garden of Indonesian Coffee and Cocoa Research Institute (ICCRI) using cocoa cake containing 26.75% originated from Forastero type of cocoa seed. The capacity and recovery of the machine was influenced by space between rotor cylinder and stator wall. Grinding machine operated at cooling temperature of 25.5oC and space between rotor – stator 0.9 cm and the capacity of 187.5 kg/hour with recovery of 200 mesh cocoa powder as much as 24%. The maximum power of machine required was 2.5 kW with efficiency of energy transfer of 97%. Results of proximate analysis showed that there was no change of protein content, but protein and carbohydrate content increased after processing, i.e. from 5.70% and 59.82% into 5.80% and 61.89% respectively.Key words : cocoa cake, cooling, grinding, cocoa powder

Inoculation of Arbuscular Mycorrhizal Fungi Increase the Growth of Cocoa and Coffee Seedling Applied with Ayamaru Phosphate Rock

Researh was carried out aimed to study the response of cocoa and coffee seedlings inoculated by Arbuscular Mycorrhizal Fungi (AMF) and applied with Ayamaru Phosphate Rock (APR). The research was cunducted at Bogor Agriculture Institute (IPB) and Papua University (Unipa) Manokwari Indonesia. Cocoa seed (Upper Amazone Hybrid F-1) and Arabica coffee seed (Coffea arabica) were collected from the Indonesian Coffee and Cocoa Research Institute (ICCI) Jember Indonesia. Cocoa dan coffee seedling were planted in polybag with acid soil medium, under 60% shading net. Cocoa and coffee seedlings were grown for 16 weeks after planting. AMF inoculum used Mycofer that was collected from IPB consisted of four species i.e Gigaspora margarita (INVAM-105), Glomus etunicatum (NPI-126), (Acaulospora tuberculata (INDO-2), Glomus manihotis (INDO-1) and indigenous AMF Manokwari consisted of three species i.e A. tuberculata Janos & Trappe, A. scrobiculata Trappe, and G. aggregatum Schenck & Smith. Ayamaru Phosphate Rock (APR) was collected from Ayamaru District, West Papua, Indonesia. The result showed that the growth of cocoa seedling applied with APR up to 4 g P2O5.seedling-1 and the growth of coffee seedling applied with APR up to 8 g P2O5.seedling-1 increased linearly. Compared to control (without AMF), inoculation of AMF Mycofer increased the cocoa seedling’s height up to 50.58% and shoot dry-weight 127.55%, whereas height of coffee seedling increased by 27.29% and shoot dry-weight by 121.21%. For all APR dosage, the growth of cocoa and coffee seedling inoculated with AMF Mycofer inoculum was better than inoculated by indigenous AMF and without AMF (control).