Individual Stalls Research Articles

Molybdenum Exposure in Drinking Water Vs Feed Impacts Apparent Absorption of Copper Differently in Beef Cattle Consuming a High-Forage Diet.

Twelve Angus steers were utilized to investigate the influence of molybdenum (Mo) in drinking water or feed on apparent absorption and retention of copper (Cu) and Mo. Steers were fed a low-quality grass hay diet for 14days. Steers were then housed in individual metabolism stalls and blocked by body weight and dry matter intake (DMI) and randomly assigned within block to one of three treatments. Treatments consisted of (1) control (no supplemental Mo), (2) 5.0mg Mo/kg DM from sodium molybdate dihydrate (Mo-diet), and (3) 1.5mg Mo/L from sodium molybdate dihydrate delivered in the drinking water (Mo-water). Total fecal and urine output were then collected for 5days. Dry matter, Cu and water intake, and DM digestibility were similar across treatments. Molybdenum intake was greater (P < 0.05) in Mo-water and Mo-diet steers when compared to controls but similar between Mo-water and Mo-diet steers. Apparent absorption and retention of Cu were greater (P < 0.05) in controls when compared to Mo-diet supplemented steers. Apparent absorption and retention of Cu in steers in the Mo-water treatment did not differ from controls or those receiving the Mo-diet. Molybdenum-diet and Mo-water supplemented steers had similar apparent absorption and retention of Cu. Apparent absorption and retention of Mo (% of Mo intake) was greater in controls when compared to Mo-supplemented steers. These data indicate that Mo consumed in water may impact Cu absorption and retention to a lesser extent than Mo supplemented in the diet.

Comparison of the Productivity of Primiparous Sows Housed in Individual Stalls and Group Housing Systems.

Simple SummaryRecently, South Korea amended the respective laws and will be enforcing that sows must be kept in group housing after 6 weeks from insemination by the year 2030. Accordingly, the comparison of productivity of sows in individual stalls and group housing systems was investigated in order to provide information on group housing systems for sows to pig farms. Primiparous sows were divided into four groups and housed in equal number in pen stalls, in short stalls with non-gated feeding stalls, in free access stalls, or with access to electronic sow feeders after 8 weeks from artificial insemination. Sows were transferred to farrowing crates at 110 days of gestation. No differences were found in sow productive performance, reproductive performance, and colostrum composition among housing types. Therefore, it was concluded that group housing systems could be used to replace individual stalls in commercial sow units.This study was conducted to provide commercial pig farms with information about group housing systems for sows in accordance with the amendment of the prohibition law for individual stalls for sows in South Korea. Therefore, this experiment was performed to compare the effects of individual stalls (IS) and group housing systems (GS) on the productivity of sows to investigate the feasibility of replacing individual stalls with group housing systems in commercial sow units. Forty primiparous sows (Landrace × Yorkshire; 210.67 ± 2.22 kg average initial body weight) were randomly assigned to four treatments with restricted feeding after 8 weeks from artificial insemination. The four treatments were (i) individual stalls (IS; housed in pen stalls), (ii) short stalls (SS; sows housed in pens with non-gated feeding stalls), (iii) free access stalls (FAS; a non-competitive housing system), and (iv) electronic sow feeders (ESF; used with radio frequency identification technology to allow individual sow management without individual confinement). All sows were transferred to farrowing crates at 110 days of gestation. There were no differences in sow productive performance, reproductive performance, and colostrum composition between IS and GS and among GS. The considered GS did not negatively affect any productivity parameters of primiparous sows compared with IS; the GS could replace IS in commercial sow units.

The Horse Gut Microbiome Responds in a Highly Individualized Manner to Forage Lignification

Although contributions of the equine gut microbiome to forage utilization are well recognized, the impact of alfalfa (Medicago sativa L.) lignification on the equine gut microbiome remains unknown; thus, we characterized microbial communities in the equine gut when feeding reduced lignin (RL) and conventional (CON) alfalfa hays to adult stock-type horses. Dietary treatments were fed to six horses in a crossover study. Experimental periods consisted of a 9-day dietary adaptation phase followed by a 5-day total fecal collection phase, during which horses were housed in individual box stalls and manure was removed on a continuous 24-hour basis. At 12-hour intervals, manure was mixed, frozen, and processed for V4, 16S rRNA amplicon MiSeq sequencing. Reduced lignin alfalfa did not shift microbiome composition equally across all horses; however, each subject’s microbiome responded to hay lignin content in an individualized manner, mostly, in terms of beta diversity. Amplicon sequence variants affiliated to Akkermansia, Fibrobacter succinogenes, Treponema, and Paludibacter fluctuated significantly when RL alfalfa was fed, with abundance patterns unique to each horse. Horse-specific associations between individual gut microbiome traits and characteristics of the digested CON or RL alfalfa were also observed, mainly in regards to dry matter digestibility and mean fecal particle size. These results indicate that the horse gut microbiome responds in an individualized manner to changes in the amount of acid detergent lignin in alfalfa hay, potentially impacting several feed digestibility characteristics. The implications of these horse-specific responses to hay lignification, for metabolic health and performance, remain to be elucidated.

Evaluation of reproductive and animal welfare parameters of swine females of different genetic lines submitted to different reproductive management and housing systems during pregnancy

ABSTRACT The aim of this study was to evaluate swine females of different genetic lines submitted to different reproductive management and housing systems during pregnancy on reproductive performance and animal welfare parameters. After artificial insemination protocol, 524 females were divided into two gestation housing systems: PEN1=animals housed in individual stalls during the breeding and after group-housed; PEN32=animals housed in individual stalls from breeding until 32 days of pregnancy and after group-housed. The number of piglets born, and the pregnancy and farrowing rates were evaluated. Welfare parameters related to the pregnancy phase were used. Females who weaned more piglets in the previous farrowing had a higher number of piglets born at the next farrowing. The pregnancy rate was affected by the number of semen doses. The farrowing rate was not influenced by the evaluated parameters, with average value of 91.36%. There was no effect of the gestation housing system and the genetic lines on pregnancy and farrowing rates, with values above 90.0%. The animal welfare indicators showed more compromised parameters in PEN1 system. PEN1 system did not impair the reproductive performance although it presented more compromised animal welfare parameters.

Comportamento ingestivo de cordeiros suplementados com níveis de glicerol em substituição ao milho

Objetivou-se com o experimento estudar os intervalos de tempo e o número de dias de observação para avaliação do comportamento ingestivo de cordeiros confinados. Utilizaram-se 25 animais castrados, com peso médio de 23,80 ± 2,0 kg. Os animais foram alojados em baias individuais e distribuídos ao acaso em cinco dietas, constituídas de volumoso (feno de capim tifton) e concentrados a base de milho, soja e níveis de glicerol, fornecidos na proporção de 60:40 (volumoso: concentrado), duas vezes por dia. O registro das atividades foi realizado durante três períodos de 24 horas, registrando o tempo despendido em alimentação, ruminação, ócio e efetuando-se a discretização dessas séries em número de período de alimentação, ruminação e ócio, e as eficiências de alimentação e ruminação da matéria seca e fibra em detergente neutro. Os tratamentos foram constituídos por: 0; 3, 6, 9 e 12% de glicerol em substituição da matéria seca do milho na dieta, e cada tratamento tinha cinco repetições. Os tempos despendidos em alimentação, ruminação e ócio e o número de período de ruminação não diferiram em nenhum dos níveis de glicerol na dieta. Porém, para o número de períodos de alimentação e ócio, apresentaram diferenças significativas. Conclui-se que a adição de glicerol nas dietas de cordeiros confinados em substituição parcial do milho, não afetou o tempo de alimentação, ruminação e ócios, a número de período de ruminação. Entretanto, pode afetar o número de período de alimentação e ócio.

Behavioral and cortisol responses to feeding frequency in pregnant sows under isocaloric intake.

The study focused on behavioral and cortisol responses to feeding frequency in pregnant sows under isocaloric intake. Twenty-four sows [(Landrace × Yorkshire); BW 216.70 ± 3.98 kg; parity 3.04 ± 0.53] were balanced for parity and randomly assigned to 1 of 3 feeding frequency regimes. Sows were fed corn–soybean meal-based diet 1× [0730 (Control), T1], 2× [half ration at 0730 and 1530 hours, T2], or 3× [one-third portion at 0730, 1130, and 1530 hours, T3] from days 30 to 60 of gestation. Sows received 7055 kcal ME/d during gestation from 2.21 kg of diet formulated to contain SID Lys/ME of 1.71 g/Mcal. Saliva samples were collected every 2 hr from 0630 to 1830 hours on day 52 and assayed for cortisol using ELISA procedure. Behavior data were collected for 7 d from day 53 of gestation by affixing a remote insights ear tag to each sow. Each sow had 120,960 data points categorized into: “Active”, “Feed,” or “Dormant”. Because of housing constraint, all sows were housed in individual stalls in the same room presenting a potential limitation of the study. The data were analyzed using PROC MIXED and GLIMMIX procedures of SAS 9.4 for cortisol and behavior count data, respectively. Sow was the experimental unit. The area under the curve (AUC) is quantitative evaluation of response as threshold varies over all possible values. The T2 sows had reduced 12-hr cortisol AUC compared with control sows (P = 0.024) and T3 sows (P = 0.004), respectively. The T2 sows had lower 3 hr (P = 0.039) and 5 hr (P = 0.015) postfeeding cortisol AUC compared with control sows. Feed anticipatory activity (FAA), 24-hr total activity, and feeding activities (eating and/or sham chewing) were reduced for T2 sows relative to the control and T3 sows (P < 0.01). Consequently, T2 sows had lower 24-hr total activity (P < 0.001) and feeding activities (P < 0.001) AUC compared with both the control and T3 sows, respectively. The T3 sows had greater FAA (P < 0.001) and 24-hr total activity AUC (P = 0.010) compared with control sows. Our data although inconclusive due to small sample size, twice daily feeding appears to be the threshold that reduces sows’ total activity AUC, feeding activity AUC, and activation of hypothalamic–pituitary–adrenal axis, reduced hunger, and exhibit potential to improve sow welfare in relation to once and thrice daily feeding regimes under isocaloric intake per kilogram live metabolic weight.

Post-operative pain behaviour associated with surgical castration in donkeys (Equus asinus).

BackgroundRecognising pain in donkeys is challenging because they are stoic.ObjectivesTo identify the responses of donkeys before and after surgical pain.Study designProspective, short‐term longitudinal pre‐ and post‐intervention observations.MethodsForty adult donkeys underwent surgical castration after sedation with intravenous (IV) xylazine, induction with guaiphenesin/thiopental IV and maintenance of anaesthesia with isoflurane and local anaesthetic blockade. Four hours after recovery from anaesthesia, flunixin meglumine 1.1 mg/kg, dipyrone 10 mg/kg and morphine 0.2 mg/kg IV were administered. Behavioural responses exhibited by the animals housed in individual stalls were recorded in four 30‐min videos: before castration (M0), and 3.5‐4.0 hours (M1), 5.5‐6.0 hours (M2) and 23.5‐24.0 hours after recovery from anaesthesia (M3). To exclude the influence of insects, the behaviour of six apparently pain‐free donkeys was compared with and without the presence of faeces and urine in the stall.ResultsWhen compared with presurgical baseline behaviours (M0), after surgery (M1) donkeys raised their pelvic limbs more (P = .003). When compared with M1, after analgesia (M2), the median frequencies of ear movements (44 vs 16; P < .001), head shaking (7 vs 1; P < .001), head turning (5 vs 0; P < .001) and lifting of the both limbs (7 vs 0; P = .008) decreased; feeding (0 vs 29; P < .001) and water intake (0 vs 0, range 0‐1 vs 0‐7; P = .05) increased. The dirty stall increased tail (53 vs 80; P = .03), head (16 vs 30; P = .03) and ear movements (50 vs 78; P = .04).Main limitationsThe dirty stall and presence of insects possibly contributed to the expression of behaviours unrelated to pain.ConclusionLifting the pelvic limbs was the only specific pain behaviour after castration in donkeys. Analgesia restored appetite and water intake and reduced the frequency of head shaking and turning, ear movement and lifting the limbs. Tail, head and ear movements are unspecific responses related both to pain and a dirty stall, and are confounding factors when pain is assessed in donkeys in the presence of insects.

Prevalência de oxiuríase em equinos do Centro Hípico do 3º Regimento de Cavalaria de Guarda do Exército Brasileiro*

The objective of this study was to determine the prevalence tax of Oxyuris equi and concomitant clinical signs in horses of the Equestrian Center of the Third Regiment of Guard Cavalry in Porto Alegre, Rio Grande do Sul. Between August and October of 2017, 93 sport horses were evaluated, of variable races, with individual stalls and exercise regimen with various intensities. The evaluated variables were: general condition of the fur coat and tail, of the perianal region and imprint of the perianal region with adhesive tape throughout Graham’s method. The prevalence tax was 25,81% (24/93) and it related to clinical signs of opaque fur coat and tail hair loss in 10,75% (10/93) and with the presence of egg masses of in 2,15% (2/93).

Some aspects of the acute phase immune response to a lipopolysaccharide (LPS) challenge are mitigated by supplementation with a Saccharomyces cerevisiae fermentation product in weaned beef calves.

This study was conducted to determine if feeding a Saccharomyces cerevisiae fermentation product (SCFP) to calves would alter the acute phase response to a lipopolysaccharide (LPS) challenge. Crossbred steer calves [n = 32; 274 ± 1.9 kg body weight (BW)] were randomly allotted to two treatment diets for 21 d: 1) control, fed RAMP (Cargill, Dalhart, TX) and 2) SCFP, fed the control ration supplemented with NaturSafe at 12 g/hd/d mixed into the TMR (NaturSafe, Diamond V, Cedar Rapids, IA). On day 22, steers were fitted with indwelling jugular catheters and rectal temperature monitoring devices and placed in individual bleeding stalls. On day 23, steers were challenged i.v. with 0.25 µg/kg BW LPS. Blood samples were collected at 0.5-h (serum) or 2-h (complete blood counts) intervals from −2 to 8 h and again at 24 h relative to the LPS challenge at 0 h. Sickness behavior scores (SBS) were recorded after the collection of each blood sample. Rectal temperatures were greater in SCFP steers from 6 to 11 h, at 13 h, from 15 to 20 h, and from 22 to 24 h following the LPS challenge compared to Control steers (treatment × time: P = 0.01). Additionally, SCFP-supplemented steers had reduced (P < 0.01) SBS compared to Control steers. Platelet concentrations remained greater in SCFP-supplemented steers compared to Control steers throughout the study (P = 0.05), while there was a tendency (P = 0.09) for SCFP steers to have greater white blood cells and eosinophils concentrations than Control steers. There was a treatment × time interaction for serum cortisol and glucose (P < 0.01). Specifically, cortisol was greater at 0.5 and 2 h postchallenge but was reduced at 3 h for SCFP steers compared to Control steers. Glucose was greater in SCFP steers at −0.5, 2, and 7.5 h compared to Control steers. Serum amyloid A was reduced in SCFP steers at 0.5 h, yet greater at 1 and 7.5 h postchallenge compared to Control steers (treatment × time: P < 0.01). Fibrinogen concentrations were greater (P < 0.01) in SCFP compared to Control steers. There was a treatment × time interaction (P < 0.01) for tumor necrosis factor-α (TNF-α) such that concentrations were reduced in SCFP steers from 1 to 2 h postchallenge compared to Control steers. Overall, these data suggest that supplementing calves with SCFP may have primed the innate immune response prior to the challenge, particularly platelets, which resulted in an attenuated sickness behavior and TNF-α response to LPS.

English

The experiment was performed in the campus of the State University of Alagoas, in Santana do Ipanema, in the semi-arid region of Northeast Brazil. This work is aimed at studying the transferences of Se from animals&rsquo; food to milk in various levels of offers and its correlations with Ca, P, Mg and soluble Fe. The work also analyzed the somatic cells counts, total bacteria count and chemical composition of milk: Fat, total soluble solids, lactose and proteins. Eight Anglo Nubians animals with a live weight of 60 kg with fifteen days of second lactation were stabled in individual stalls and confined in a completely randomized design, DIC. The animals were fed with 2 kg.day-1 of cactus pear and 0.5 to 1.0 kg.day-1 Tifton hay (Cynodon spp.); concentrated and formed with 333.3 g.day-1 corn and 166.67 g.day-1 soy. The mineral salt was dosed in three treatments using selenium (Se). The treatments were; Treatment 00: at 0.0 mg kg-1 of animal MS.day-1 serving as control group with no supply of mineral salt, and if provided, it came from their own diet; Treatment 01: at 0.1 mg MS.day-1 of animal.kg-1; Treatment 02: at 0.45 mg of animal. kg-1 DM. day-1; and Treatment 03: with 0.90 mg Se animal.kg-1 DM.day-1 (p &lt;0.05) using Tukey test. Key words: Calcium, phosphorus, magnesium, soluble iron. &nbsp

Evaluation of methodologies of urine collection to estimate microbial synthesis in bovines diets containing or not containing lipids

This study investigated the effectiveness of the urine sample collection method in predicting the volume urinary and synthesis of microbial nitrogen. Eight fistulated steers were used with accessible rumens and kept in individual stalls. Their diets consisted of corn silage; corn silage + concentrate; corn silage + concentrate with addition of lipids in the form of soybean oil; and corn silage + concentrate with addition of lipids in the form of soybean grains. Estimates of microbial protein synthesis were obtained based on the urinary excretion of purine derivatives. There was no effect of diets on daily creatinine excretion (P&gt;0.05). There were differences (P&lt;0.05) between the urinary volume and microbial synthesis values determined by the total urine collection and those estimated from the urine spot samples and equations proposed by different authors. The estimation of microbial synthesis based on the urine excretion of purine derivatives should be performed from the total collection of the urine for a period of 24 hours.

Intake, nitrogen balance and microbial protein synthesis of steers fed with or without lipid addition

This study investigated the influence of energy supplementation with or without the addition of lipids on microbial production, microbial synthesis efficiency and nitrogen balance. Eight fistulated steers were used with accessible rumens and kept in individual stalls. Their diets consisted of corn silage; corn silage + concentrate; corn silage + concentrate with addition of lipids in the form of soybean oil; and corn silage + concentrate with addition of lipids in the form of soybean grains. Estimates of microbial protein synthesis were obtained based on the urinary excretion of purine derivatives. The concentrations of ammonia in the rumen were determined immediately at 2, 4, 6 and 8 hours after feeding. The diets with concentrate increased (P&lt;0.05) the microbial protein synthesis and the efficiency of the synthesis and nitrogen balance without a difference between the lipid sources (P&gt;0.05). Concentrated diets presented higher concentrations of urea nitrogen in the serum and urinary urea excretion (P&lt;0.05), but there was no difference between the lipid sources (P&gt;0.05). Energy supplementation, with or without lipid addition, can be used as a strategy to increase the synthesis of the microbial protein in the cattle fed corn silage.

Welfare benefits after the implementation of slow-feeder hay bags for stabled horses

Horse confinement is a common practice worldwide. However, preventing horses from grazing and denying them social interaction can compromise their welfare and lead to undesirable consequences. Considering the hypothesis that increasing the forage consumption time can lead to improved welfare, the objective of this study was to verify the effect of the implementation of a slow-feeder hay bag on the ethogram, motor activity, heart rate, and cortisol circadian rhythm (CCR) of stabled horses. Seven healthy horses were kept exclusively in individual stalls and fed pelleted feed, alfalfa hay, and Tifton hay. Horses were evaluated before the experiment and for 10 days foraging through a slow-feeder hay bag. The horses were monitored for 24 hours for ethogram and forage consumption time evaluations. The CCR was calculated by the percentage variance between the morning and afternoon serum concentration. A variation of 30% or less was indicative of poor welfare. The effect of time over the variables was verified by paired Student's t or Student-Newman-Keuls tests (P < 0.05). The inclusion of hay bags increased the alfalfa ingestion time by 87% (P = 0.006). At the end of the study, coprophagia stopped in three of four horses and the mean (±SD) time spent performing abnormal behaviors decreased from 130.6 (±168.8) to 86 (±140) min (P = 0.02). The mean CCR variation increased from 26% (±24) to 58% (±9) (P = 0.006), with an incidence of four horses with abnormal CCR at the baseline and none at the end of the experiment. The motor activity was reduced from 574 (±126) to 306 (±167) steps per day (P = 0.05) and the heart rate did not vary. In conclusion, the adoption of a slow-feeder bag, an available and low-cost device, was effective in improving the welfare of stabled horses.

Trough half empty: Pregnant sows are fed under half of their ad libitum intake

AbstractPregnant (dry) sows (Sus scrofa) are fed a rationed amount of feed to maintain healthy weight and production but this does not satisfy their hunger. This study measured the extent of feed restriction compared to sows’ desired intake. Forty-seven Large White × Landrace sows were housed in small groups with straw bedding and individual feeding stalls. Following three days on a standard ration of 2.5 kg, they were offered 10 kg a day of commercial dry sow feed for three days, split into four 2.5-kg meals a day which enabled individual intakes to be measured. This quantity was effectively ad libitum (maximum daily intake 9.4 kg). Mean (± SEM) intake per day over the three ad libitum days was 5.67 (± 0.24) kg, compared to the 2.5-kg standard ration. The ration thus provides less than half (44.1%) of sows’ desired intake. Behaviour on their third rationed day was compared with behaviour on the third day of ad libitum. Eating rate and the display of hunger-related behaviours, particularly following the morning feed, was greater under ration feeding; sows spent more time in the food stall and less in the straw bed, and more time active rather than resting. During ration-feeding sows also chewed and nosed more at straw bedding and pen equipment and used the drinker more after their morning meal than when they were fed ad libitum. Eating rate on the last rationed day was positively correlated with feed intake on each of the ad libitum days. Despite an EU requirement for fibre to be added to diets to ameliorate this problem, and the provision of straw bedding, hunger resulting from food restriction remains a welfare concern for dry sows.

Technical note: Method for improving precision of in-parlor milk meters and adjusting milk weights for stall effects

Milk yield is a fundamental observation in most dairy experiments and is commonly determined using integrated milk meters that measure milk weight as the cow is being milked. These meters are heavily used in a harsh environment and often are not regularly calibrated, so calibration errors and mechanical problems may create artificial variation in milk weight data. Additionally, direct calibration by collection of milk in a bucket is difficult and imperfect because the use of the bucket may affect yield recorded by the milk meter. The objective of this work was to define a method to easily check parlor meter precision and adjust milk weight values for variation between individual stalls in a parlor. Because most cows are milked in a different stall at each milking, it has been proposed that stall deviations that represent the fixed effect of stall on milk weight could be statistically determined. Individual milk weights from 14 milkings across 7 d from approximately 200 cows were collected from the Penn State dairy farm, which is equipped with a double-10 herringbone parlor with an Afimilk 2000 milking system (S.A.E. Afikim, Afikim, Israel). Milk yield was measured automatically by in-line flow through milk meters (Afi 200; S.A.E. Afikim). The effect of stall on milk weight was modeled using a mixed model that included the fixed effect of stall and the random effects of day, milking time, and cow. First, stall deviations were calculated as the stall least squares means (LSM) minus the average LSM to identify malfunctioning meters requiring service (e.g., deviation exceeding 1 kg). A correction factor for each stall was then generated by dividing the LSM of each stall by the average LSM. Milk yields were then corrected by multiplying the meter weight value by the correction factor. To determine the effect of the correction, raw and corrected meter values were compared with weight of milk collected in a bucket (n = 3/stall). The corrected values had a 5% greater coefficient of determination than raw meter values (0.89 vs. 0.84) and had a lower average percent difference from the bucket milk weight compared with raw meter values (12.6% vs. 13.5%). The method was then used in 3 experiments with 121, 140, and 683 milk yield observations. In all data sets, correcting milk weights slightly improved model fit and had minimal effect on model term standard errors. However, this validation was completed in a parlor where the method was routinely used to identify stalls requiring service; the effect of stall corrections is expected to be larger in parlors without frequent monitoring. Stall deviations are expected to be due predominantly to calibration of the meter but also could be due to differences in pulsation or other stall-specific factors that result in a change in milk yield. It is important to account for these other sources of milk weight variation that are unrelated to treatment. Modeling the effect of stall is a simple, convenient, and low-cost method to monitor and improve milk meter precision and functionality and can be used to reduce artificial variation and experimental error.

Sugarcane with Elephant grass replacing sugarcane with urea in the diet of crossbred dairy cows1

The objective was to evaluate the partial substitution of sugarcane treated with urea by elephant grass in the feeding of crossbred cows in lactation. Eight crossbred cows (Holstein x Gyr) were used, with 474 kg of body weight (standard deviation = 46.15 kg) and 8.15 kg of milk per day. The animals were housed in individual stalls, distributed in two 4 x 4 Latin squares, and the treatments were: sugar cane; sugar cane corrected with 0.5% of urea and ammonium sulfate (9:1); 75% of sugar cane + 25% of elephant grass and 50% of sugarcane + 50% of elephant grass, based on natural matter. In addition to the treatments, it was provided water and mineral salt ad libitum and 3 kg of concentrate supplement (24% crude protein), based on corn meal and soybean meal, supplied in two portions during the morning and afternoon milking. There was a treatment effect on the intake and digestibility of dry matter and nutrients, except for non-fibrous carbohydrate intake, and there was no effect on milk production and composition. It can be concluded that the substitution of sugarcane by elephantgrass (25 and 50%) increases the intake and decreases the digestibility of DM and OM, without affecting milk production, when the cows are supplemented with 3 kg of concentrate, containing 24% of protein per day.

Plasma fatty acid profile in dairy cows associated with the inclusion of annatto in their diet

The objective of this study was to evaluate the plasma lipid profile and plasma fatty acids of dairy cows receiving diets supplemented with annatto. A total of 32 Holstein cows (550 kg), distributed in a completely randomized design, were allocated to individual stalls and submitted to following treatments: C0 = no annatto; C4 = inclusion of annatto at 4 g kg-1 dry matter (DM) of diet (0.07 g bixin kg-1 diet); C5 = inclusion of annatto at 5 g kg-1 DM of diet (0.09 g bixin kg-1 diet); and C7 = inclusion of annatto at 7 g kg-1 DM of diet (0.12 g bixin kg-1 diet). Blood samples were collected via epigastric vein puncture, centrifuged, and frozen for subsequent analysis. The results indicate that the inclusion (p &gt; 0.05) of annatto does not decrease the total cholesterol or low and high density lipoproteins. However, it impacts the profile of fatty acids, evidenced by the reduction (p &lt; 0.05) in levels of hypercholesterolemic fatty acids viz, myristic acid and palmitic acid. It also causes an increase in the levels of arachidonic acid, rumenic acid, linoleic acid, and total polyunsaturated fatty acids. Therefore, bixin included in the diets of dairy cows induces changes in the plasma fatty acid profile.

Characterization of Boar Studs in Brazil

ABSTRACT: Understanding the flow of processes in swine production systems and how they work is fundamental to improving reproductive performance. We surveyed 32 boar studs in Brazil, representing 61.53% of the total in the country. Commercial lines were the most common breed (59.38%) used in the studs. Individual pens and stalls were the most commonly used as housing system (71.9%), and 81.25% of the studs reported having some form of thermal control system. For most of the studs (62.51%), average weekly sperm concentration was higher than 200 million cells per mL. Also, for most studs (71.88%) average weekly ejaculate volume was more than 250 mL. In 46.88% of the studs each ejaculate yielded 26 to 40 semen doses. In 6.25% of the studs, 3.5 billion sperm cells per dose were used for artificial, intracervical insemination. Sperm concentration in 46.88% of the studs was determined using a computer-assisted system analysis. The assessment of sperm quality was conducted using morphology, concentration, and microbiological testing. Employees working on semen collection had formal education of elementary/middle school (34.38%) and high school (37.5%). Most of the laboratory technicians had frequented high school (75%). The most time-consuming task was semen processing, taking 16 to 25 h a week (46.8%) and 6 to 10 h was allotted for the cleaning of stud facilities (46.8%). The data collected in the present study allow greater knowledge of this important part in the pig production chain in Brazil.

Meat quality and lipid profile of intramuscular fat of Texel crossbred lambs classified by residual intake and gain

Context Many indices of feed efficiency for livestock have been proposed, but most are associated with differences in body composition and consequently affect meat quality. Aims This study aimed to assess meat quality and lipid profile of intramuscular fat of Texel crossbred lambs classified by residual intake and gain (RIG). Methods Seventy-seven non-castrated male Texel crossbred lambs (1/4 Pantaneira and 3/4 Texel) were assessed in a 2-year study. First year: Forty-seven lambs with initial bodyweight of 29.9 ± 5.5 kg. Second year: Thirty lambs with an initial bodyweight of 22.4 ± 3.3 kg. Lambs were confined in individual stalls for 70 days for the assessment of individual dry matter intake and average daily gain, being then slaughtered. The muscle Longissimus lumborum was removed for analyses. The RIG was obtained by: RIG = (−1 × residual feed intake) + residual gain. The data of meat quality and lipid profile from both years were grouped and analysed in a completely randomised design. Animals were classified into three RIG classes based on the s.d. of RIG variable. Efficient (n = 20) and inefficient (n = 22) animals were classified adopting ± 0.5 s.d. from RIG mean, and animals between these two groups were classified as intermediate (n = 35). Key results No significant differences were observed between RIG classes regarding instrumental characteristics and proximate composition of the Longissimus lumborum muscle. We observed no differences between RIG classes for concentrations of saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA), as well as for the total sum of SFA, MUFA, and PUFA of intramuscular fat, with 40.9, 54.1, and 5.1% of total FA, respectively. The MUFA:SFA ratio, PUFA:SFA ratio, atherogenic and thrombogenic index were also not influenced by RIG classes. Conclusions There were no differences in meat quality and lipid profile of intramuscular fat between animals classified based on RIG. Implications Improvements provided by using RIG to identify efficient and faster-growing lambs could assist in reducing feed and labour investments in the production system through an early slaughter without having negative impacts on meat quality.

Short-Term Eating Preference of Beef Cattle Fed High Forage or High Grain Diets Supplemented with 3-Nitrooxypropanol.

Simple Summary3-Nitrooxypropanol is an effective methane-mitigating feed additive. However, a decrease in feed intake is frequently observed in beef cattle fed a diet supplemented with 3-nitrooxypropanol. Thus, a study was conducted to determine the short-term preference of beef cattle offered diets supplemented with 3-nitrooxypropanol. When given a choice, the cattle initially showed less preference for a diet supplemented with 3-nitrooxypropanol, suggesting potential changes in organoleptic properties of the diet. However, the animals acclimatized to the supplemented diet rapidly such that there was no preference between a diet with and without 3-nitrooxypropanol within a week. Therefore, the change in the organoleptic properties of a diet supplemented with 3-nitrooxypropanol does not appear to be the main reason for reduced feed intake of beef cattle.Two experiments were conducted to examine eating preference of beef cattle for diets with or without the investigative enteric methane inhibitor 3-nitrooxypropanol (3-NOP). Nine beef steers were housed in individual stalls, each equipped with two feed bunks. The first experiment (Exp. 1) was conducted with a high forage diet and each animal received a diet without 3-NOP (CON) in one bunk and a diet with 3-NOP (dNOP) in the other bunk. The second study (Exp. 2) was conducted with the same animals about 6 months after Exp. 1 where a high grain diet without (CON) or with 3-NOP (dNOP) was offered. In Exp. 1, animals initially preferred CON compared with dNOP. Feed consumption from 0 to 3, 3 to 6, and 6 to 12 h after feeding was lower for dNOP compared with CON. However, dry matter intake (DMI) and feed consumption of dNOP gradually increased during Exp. 1 such that there was no preference between CON and dNOP on day 7. In Exp. 2, there was no preference for or against dNOP. Average DMI was greater for dNOP vs. CON, but interactions between diet and day for DMI and feed consumption rates indicated that daily preference between CON and dNOP was variable. In conclusion, beef steers initially detected a difference between CON and dNOP and selected in favor of CON rather than dNOP when they had not previously been exposed to 3-NOP. However, the animals rapidly acclimatized to a diet with 3-NOP (Exp. 1) and showed no eating preference between CON and dNOP within 7 days. This lack of preference was maintained throughout Exp. 2 when the same animals were fed a high grain diet.