Individual Sites Research Articles

Physical environment, species choice and spatio-temporal patterns of urban roadside trees in Hong Kong

Trees in the urban environment are affected by a range of place-specific environmental and cultural factors. Nevertheless, many factors interact with each other and produce high variabilities in urban forest structures, spatial configurations, and growing conditions within the urban fabric. The variabilities and relationships among these factors require a modelling framework that incorporates variables related to the spatio-temporal information of individual trees and site characteristics. Using roadside trees in a residential district in Hong Kong, this study examined their species compositions and spatial distributions among zones with various development histories. Structural equation modelling was applied to investigate the interactions among variables concerning the physical environment, temporal changes, species selection, spatial patterns, and tree sizes. A few abundant species dominated the urban forest, with uneven distributions of roadside tree densities and species-specific clusters found within the study area. The empirical results showed (i) the physical environment influenced the existence of roadside trees, the occurrence of specific species and tree growth; (ii) species selection mediated the influences of the physical environment on tree sizes and spatial patterns; (iii) despite the linkage between existing tree patterns and development histories, replacement plantings further modified the compositions and reflected a shift in preferences; (iv) the proximity to nearby urban structures and the spatial properties among planting locations were important factors in spatial planning. Hence, urban forest managers play a crucial role in mediating the interactions among physical drivers, planting histories, and tree performances. It is recommended that species diversity, especially native trees, should be promoted within the study area, while planting strategies matching site dimensions, species and attainable sizes should also be prioritised. By generalising the relative effects that determined the existing patterns of urban forests, the modelling framework can provide a strategic reference for target-oriented tree management in Hong Kong and other cities.

Quantifying aspects of rangeland health at watershed scales in Colorado using remotely sensed data products

• During grazing permit renewals, the Bureau of Land Management assesses land health using indicators typically measured using field-based data collected from individual sites within grazing allotments. However, agency guidance suggests assessments be completed at larger spatial scales. • We explored how the current generation of remotely sensed data products could be used to quantify aspects of land health at watershed scales in Colorado to provide broad spatial and temporal context for the land health assessment process. • We found multiple indicators could be quantified using these data products and were relevant to land health standards. • Within focal watersheds, bare ground cover decreased over the past 30 years, while annual herbaceous cover has increased over the last 10 years. Vegetation productivity was variable over time, but interannual fluctuations were consistent across watersheds. • Remotely sensed data products can help resource managers understand how current conditions relate to broad spatial and temporal trends in the region and could provide another line of evidence for the land health assessment process. They may also identify target areas where management strategies, such as eradication of invasive annual grasses, should be focused, and could help resource managers communicate complex issues to the public.

Factors in the Responsible Management of the Luna Valley Complex Geosite (NW Spain)—A Case Study

The Luna Valley complex geosite (northwestern Spain) is a region of geoheritage significance located in an area with high environmental value. Geological studies began in the mid-20th century and continue to provide scientific data of significant relevance to the knowledge regarding the Palaeozoic stratigraphy of northern Gondwana and the tectonics of the Variscan orogen. This region also has high value for geoeducation, being visited regularly by both students and the general public. Educational use of the area has promoted the creation of several publicly available materials and activities that include trails, guides, displays and brochures, as well as the development of a small museum. However, over time, weathering; the abandonment of rural life; and the intensive, uncontrolled, and careless use of this region as a geosite for scientific and educational purposes has led to significant degradation and the consequent loss of its geoheritage value. This paper describes the geology of five key geosites in the Luna Valley. This is followed by a review of the promotional initiatives carried out in the area. These data, along with our knowledge of the area, allow us to develop a heritage analysis that includes the main geological interests, conservation status and some key management issues for each of these five individual sites. Several recommendations aim to control the physical degradation of the geosites, encourage their regular monitoring and the updating of the outreach materials using virtual tools, and promote the involvement of the local population in the conservation of this unique site.

Does hatchery rearing of lake trout affect their reproductive behavior in the wild?

Stocking of hatchery-reared fishes has been used with variable success as a management action to promote the recovery of populations and species. The practice has been controversial for several reasons, including uncertainty about whether the hatchery rearing experience may affect reproduction after release. Fine-scale acoustic telemetry was used during three spawning seasons to test whether hatchery rearing affects the reproductive behavior of lake trout using a spawning shoal complex in northern Lake Huron. Within sex, wild- and hatchery-reared fish behaved similarly, but significant behavioral differences occurred between sexes. Lake trout of both sexes moved synchronously onto the spawning shoals at the completion of autumn thermal turnover and occupied the same spawning sites (confirmed visually by presence of fertilized eggs) on the shoals. Male lake trout tended to congregate directly on spawning sites, with duration of occupancy varying greatly among years. Female lake trout spent less time on spawning shoals than males and congregated less at spawning sites on shoals. Most fish visited multiple spawning sites among shoals per season, with many making multiple transits among individual spawning sites. We found no evidence to support the hypothesis that hatchery rearing impairs spawning behavior of lake trout and, therefore, conclude that behavior deficiencies on the spawning ground are likely not an impediment to rehabilitation of lake trout in northern Lake Huron. Our study narrows the field of possible impediments to lake trout rehabilitation in the Great Lakes and provides insights that expand the conceptual model of lake trout spawning behavior.

A comprehensive evaluation of the use of Lagrangian particle dispersion models for inverse modeling of greenhouse gas emissions

Abstract. Using the example of sulfur hexafluoride (SF6), we investigate the use of Lagrangian particle dispersion models (LPDMs) for inverse modeling of greenhouse gas (GHG) emissions and explore the limitations of this approach. We put the main focus on the impacts of baseline methods and the LPDM backward simulation period on the a posteriori emissions determined by the inversion. We consider baseline methods that are based on a statistical selection of observations at individual measurement sites and a global-distribution-based (GDB) approach, where global mixing ratio fields are coupled to the LPDM back-trajectories at their termination points. We show that purely statistical baseline methods can cause large systematic errors, which lead to inversion results that are sensitive to the LPDM backward simulation period and can generate unrealistic global total a posteriori emissions. The GDB method produces a posteriori emissions that are far less sensitive to the backward simulation period and that show a better agreement with recognized global total emissions. Our results show that longer backward simulation periods, beyond the often used 5 to 10 d, reduce the mean squared error and increase the correlation between a priori modeled and observed mixing ratios. Also, the inversion becomes less sensitive to biases in the a priori emissions and the global mixing ratio fields for longer backward simulation periods. Further, longer periods might help to better constrain emissions in regions poorly covered by the global SF6 monitoring network. We find that the inclusion of existing flask measurements in the inversion helps to further close these gaps and suggest that a few additional and well-placed flask sampling sites would have great value for improving global a posteriori emission fields.

The Role of Serotonergic Gene Methylation in Regulating Anxiety-Related Personality Traits in Chimpanzees.

While low serotonergic activity is often associated with psychological disorders such as depression, anxiety, mood, and personality disorders, variations in serotonin also contribute to normal personality differences. In this study, we investigated the role of blood DNA methylation levels at individual CpG sites of two key serotonergic genes (serotonin receptor gene 1A, HTR1A; serotonin transporter gene, SLC6A4) in predicting the personalities of captive chimpanzees. We found associations between methylation at 9/48 CpG sites with four personality dimensions: Dominance, Reactivity/Dependability, Agreeableness, and Openness. Directionality of effects were CpG location-dependent and confirmed a role of serotonergic methylation in reducing anxiety (Dominance) and aggression-related personality (Reactivity/Undependability) while simultaneously promoting prosocial (Agreeableness) and exploratory personalities (Openness). Although early-life adversity has been shown to impact serotonergic methylation patterns in other species, here, atypical early social rearing experiences only had a modest impact on CpG methylation levels in this chimpanzee sample. The precise environmental factors impacting serotonergic methylation in chimpanzees remain to be identified. Nevertheless, our study suggests a role in shaping natural variation in animal personalities. The results of this study offer a basis for future hypothesis-driven testing in additional populations and species to better understand the impact of ecology and evolution on complex behavioral traits.

Harmonization of Rapid Evaporative Ionization Mass Spectrometry Workflows across Four Sites and Testing Using Reference Material and Local Food-Grade Meats.

Rapid evaporative ionization mass spectrometry (REIMS) is a direct tissue metabolic profiling technique used to accurately classify tissues using pre-built mass spectral databases. The reproducibility of the analytical equipment, methodology and tissue classification algorithms has yet to be evaluated over multiple sites, which is an essential step for developing this technique for future clinical applications. In this study, we harmonized REIMS methodology using single-source reference material across four sites with identical equipment: Imperial College London (UK); Waters Research Centre (Hungary); Maastricht University (The Netherlands); and Queen's University (Canada). We observed that method harmonization resulted in reduced spectral variability across sites. Each site then analyzed four different types of locally-sourced food-grade animal tissue. Tissue recognition models were created at each site using multivariate statistical analysis based on the different metabolic profiles observed in the m/z range of 600-1000, and these models were tested against data obtained at the other sites. Cross-validation by site resulted in 100% correct classification of two reference tissues and 69-100% correct classification for food-grade meat samples. While we were able to successfully minimize between-site variability in REIMS signals, differences in animal tissue from local sources led to significant variability in the accuracy of an individual site's model. Our results inform future multi-site REIMS studies applied to clinical samples and emphasize the importance of carefully-annotated samples that encompass sufficient population diversity.

Selective retention of virus-specific tissue-resident T cells in healed skin after recovery from herpes zoster

Herpes zoster is a localized skin infection caused by reactivation of latent varicella-zoster virus. Tissue-resident T cells likely control skin infections. Zoster provides a unique opportunity to determine if focal reinfection of human skin boosts local or disseminated antigen-specific tissue-resident T cells. Here, we show virus-specific T cells are retained over one year in serial samples of rash site and contralateral unaffected skin of individuals recovered from zoster. Consistent with zoster resolution, viral DNA is largely undetectable on skin from day 90 and virus-specific B and T cells decline in blood. In skin, there is selective infiltration and long-term persistence of varicella-zoster virus-specific T cells in the rash site relative to the contralateral site. The skin T cell infiltrates express the canonical tissue-resident T cell markers CD69 and CD103. These findings show that zoster promotes spatially-restricted long-term retention of antigen-specific tissue-resident T cells in previously infected skin.

Abstract PR005: Primary results of PanCAN-SR1, a phase 1b study evaluating Gemcitabine, nab-Paclitaxel, Canakinumab, and Spartalizumab to target IL-1β and PD-1 in metastatic pancreatic cancer with correlative tissue and blood biomarker analysis

Abstract In preclinical work, the inflammatory cytokine IL-1β was shown to be upregulated in pancreatic cancer tumors and to contribute to activation of pancreatic stellate cells and immunosuppression (Das et al 2020). We conducted an open-label multicenter Phase Ib study evaluating gemcitabine, nab-paclitaxel, canakinumab (ACZ885), a high-affinity human anti-interleukin-1β (IL-1β) mAb, and spartalizumab (PDR001), a PD-1 mAb. Eligible subjects had previously untreated metastatic PDA and RECIST measurable disease. The primary objective was to confirm recommended phase II dose by evaluating the incidence of dose limiting toxicities (DLTs) in the first 56 days of dosing in at least 6 evaluable subjects utilizing a Bayesian logistic regression model. Subjects underwent baseline and on-study tissue and blood collection for correlative translational research. Results: 10 subjects were enrolled between Nov 2020 and Mar 2021. At the primary data cut off of May 23, 2021, 6 subjects were evaluable for DLT. There were no DLTs, the recommended Phase II dose was established as: gemcitabine (1000mg/m2 IV) day 1,8,15; nab-paclitaxel (125mg/m2 IV) day 1,8,15; canakinumab (250mg SC) day 1, spartalizumab (400mg IV) day 1; of each 28 day cycle. At the time of an updated database extraction on June 1, 2022, 2 subjects remain on study. Adverse events were consistent with those typically seen with chemotherapy. The most common Grade 3/4 AEs were neutropenia (60%) and anemia (50%), with no fatal AEs. One patient discontinued spartalizumab due to grade 3 pneumonitis. In preliminary efficacy analysis (n=10), there are 3 confirmed PRs, 5 subjects with stable disease, 2 subjects with progression as best response. Individual site data estimates that the 12 month OS rate is 60%; updated RR, PFS and OS data will be reported. Activation of CD8 T cells in peripheral blood and increased serum levels of IFN-induced chemokines CXCL9/10 were observed in both responder and non-responder patients. Using an in vitro suppression assay, we showed that baseline serum from responders could induce myeloid derived suppressor cells, an effect that was abrogated with treatment. Single cell transcriptional profiling, multiplex immunofluorescence and spatial transcriptomics also revealed treatment-dependent shifts in T cell activation state and myeloid cells in the tumors of patients experiencing clinical response. Conclusions: PanCAN-SR1 established the Phase II dose of canakinumab and spartalizumab with chemotherapy in first line metastatic PDA, based upon favorable benefit-risk assessment. Based on our comprehensive analysis of 10 patients treated with combination therapy including IL-1b blockade, we hypothesize that the definitive role of anti-IL-1b in human patients with pancreatic cancer is to reduce systemic immune suppression and to reduce immunosuppressive myeloid cell activation in the tumor. The clinical utility of targeting IL-1β in pancreatic cancer is being evaluated in a randomized Phase II/III study through the Precision Promise clinical trial network. NCT04581343. Citation Format: Paul E. Oberstein, Osama Rahma, Nina Beri, Amy Stoll-D'Astice, Emily A. Kawaler, Igor Dolgalev, Gregor Werba, Victoire Cardo-Ruffino, Naïma Böllenrücher, Andressa Dias Costa, Saloney Nazeer, Matthew Squires, Jonathan Nowak, Dafna Bar-Sagi, Brian Wolpin, Diane M Simeone, Stephanie K Dougan. Primary results of PanCAN-SR1, a phase 1b study evaluating Gemcitabine, nab-Paclitaxel, Canakinumab, and Spartalizumab to target IL-1β and PD-1 in metastatic pancreatic cancer with correlative tissue and blood biomarker analysis [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer; 2022 Sep 13-16; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2022;82(22 Suppl):Abstract nr PR005.

Evaluation of grain yield performance and its stability in various spring barley accessions under condition of different agroclimatic zones of Ukraine

Two extremely urgent problems of biological and agronomic research nowadays are ensuring an optimal balance between usage of natural resources to meet rapidly growing needs for food production and preservation of biodiversity. It is also important to extend the genetic diversity of the main crop varieties in agroecosystems. At the same time, modern varieties should be characterized by a combination of high yield and preserving yield stability under variable conditions. Solving the outlined tasks requires comprehensive research and involvement in breeding process of the genetical diversity concentrated in genebanks of the world. Barley (Hordeum vulgare L.) is one of the most important crops that satisfy the various needs of humanity. In respect to this, in 2020–2022, a multi-environment trial was conducted in three agroclimatic zones of Ukraine (Forest-Steppe, Polissia, and Northern Steppe). We studied 44 spring barley collection accessions of different ecological and geographical origin, different subspecies and groups of botanical varieties which were obtained from the National Center for Plant Genetic Resources of Ukraine. Statistical indices (Hom, Sc) and graphical models (GGE biplot, AMMI) were used to interpret the yield performance and its stability. Both individual ecological sites in different years and combinations of different sites and years of trials were characterized for productivity, discriminating power and representativeness. The environments differed quite strongly among themselves in terms of these indicators. It was established that most of the genotypes were characterized by higher adaptability to individual environmental conditions (stability in different years), compared to adaptability for all agroclimatic zones (wide adaptation). A strong cross-over genotype by environment interaction was found for most studied accessions. Nevertheless, both genotypes with very high stability in only one agroclimatic zone (Amil (UKR), Gateway (CAN)) and genotypes with a combination of high adaptability to one or two ecological niches and relatively higher wide adaptability (Stymul (UKR), Ly-1064 (UKR), Rannij (KAZ), Shedevr (UKR), and Arthur (CZE)) were identified. There were also the accessions which did not show maximum performance in the individual sites, but had relatively higher wide adaptability (Ly-1059 (UKR), Ly-1120 (UKR), Diantus (UKR), and Danielle (CZE)). In general, the naked barley genotypes were inferior to the covered ones in terms of yield potential and wide adaptability, but at the same time, some of them (CDC ExPlus (CAN), CDC Gainer (CAN), and Roseland (CAN)), accordingly to the statistical indicators, had increased stability in certain ecological sites. Among naked barley accessions relatively better wide adaptability according to the graphical analysis was found in the accession CDC McGwire (CAN), and by the statistical parameters CDC ExPlus (CAN) was better than standard. The peculiarities of yield manifestation and its variability in different spring barley genotypes in the multi-environment trial revealed in this study will contribute to the complementation and deepening of existing data in terms of the genotype by environment interaction. Our results can be used in further studies for developing spring barley variety models both with specific and wide adaptation under conditions of different agroclimatic zones of Ukraine. The disitnguished accessions of different origin and botanical affiliation are recommended for creating a new breeding material with the aim of simultaneously increasing yield potential and stability, as well as widening the genetic basis of spring barley varieties.

Investigations on a novel biologically active organic molecule of 5-Chloro-1-(4-piperidyl)-2-benzimidazolinone by spectroscopic, molecular docking, and quantum chemical approach

The FT-IR, FT-Raman, UV-Vis, 1H, and 13C NMR spectroscopic, molecular structure, and electronic behavior of 5-Chloro-1-(4-piperidyl)-2-benzimidazolinone (CPBI) molecule was studied in detail. The quantum computational calculations were performed by the DFT/ B3LYP method using the 6-311++G (d,p) basis set. The vibrational wavenumbers and NMR chemical shifts were calculated and compared with the experimental. A PES scan study was carried out to find the stable conformer of the CPBI molecule. The Fukui function was calculated through Mulliken population analysis (N, N+1, and N-1) to predict individual atoms' global softness and reactive sites in the CPBI molecule. MEP map reveals that the reactive sites for electrophilic and nucleophilic attack and the lower HOMO- LUMO energy gap explains that the title molecule was more reactive and least stable. The inter-and intra-molecular interactions were identified through NBO analysis, and the presence of π → π* delocalization interaction within the molecular system stabilizes the CPBI molecule. The nonlinear optical property was also calculated for the title molecule. Lipinski's rule of five and bioavailability score show that the CPBI molecule was a good candidate for protein-ligand interaction and may be suitable for the new drug design process. Finally, molecular docking studies showed that the title molecule exhibits better inhibition activity against oncology, asthma, thrombotic and cardiovascular diseases.

Habitat associations of imperilled fishes after conservation intervention in the Cape Fold Ecoregion, South Africa.

The aim of this study was to examine the habitat associations of a native cyprinid community of the recovering Rondegat River in the Cape Fold Ecoregion of South Africa as part of a long-term native fish abundance monitoring project. Relative abundance data were extracted from underwater video camera footage across the longitudinal gradient of the river in three sampling instances. Using multivariate methods the authors assessed community composition with respect to habitat, its overlap with a protected area and species-specific abiotic predictors of relative abundance. Distance from the uppermost site in the river was the most significant predictor of species abundance, indicating spatial segregation and varying overlap between species. The protected status of sites in the upper reaches, vegetated substrates and the size of individual sites were the most impactful on the relative abundance of the endangered fiery redfin Pseudobarbus phlegethon. The results of this study indicate that underwater video monitoring is an effective and low-cost approach that can inform conservation recommendations. Reducing agricultural runoff and sedimentation in the lower reaches may be useful further interventions to maintain key habitats of submerged vegetation.

Dynamic propagation of mode III cracks in a Lattice Boltzmann method for solids

This work presents concepts and algorithms for the simulation of dynamic fractures with a Lattice Boltzmann method (LBM) for linear elastic solids. This LBM has been presented previously and solves the wave equation, which is interpreted as the governing equation for antiplane shear deformation. Besides the steady growth of a crack at a prescribed crack velocity, a fracture criterion based on stress intensity factors has been implemented. This is the first time that crack propagation with a mechanically relevant criterion is regarded in the context of LBMs. Numerical results are examined to validate the proposed method. The concepts of crack propagation introduced here are not limited to mode III cracks or the simplified deformation assumption of antiplane shear. By introducing a rather simple processing step into the existing LBM at the level of individual lattice sites, the overall performance of the LBM is maintained. Our findings underline the validity of the LBM as a numerical tool to simulate solids in general as well as dynamic fractures in particular.

Mercury in Kansas Fish: Levels, Patterns, and Risk-Based Safe Consumption Limits for Mercury Sensitive Individuals

In wet environments a portion of ambient inorganic mercury (Hg) is transformed to methylmercury (MeHg); a neurotoxin that readily concentrates in fish. Public health entities monitor Hg levels in fish to inform consumers of the types and amounts of fish that are safe to eat. In this study, monitoring data collected from stream and lakes across Kansas were used to summarize fillet tissue total mercury (THg = MeHg + Hg) levels in 36 fish species and hybrids. Data associated with eleven food fish taxa (genus and species) were presented in detail and statewide risk-based monthly safe consumption limits (SCLs) protective of Hg sensitive individuals were calculated, including two size categories (< or ≥ 508 mm) for larger growing taxa. Linear mixed effects models (LMEMs) were used to generate least squares (LS) mean fish THg values for between habitat (lakes and streams) and within habitat (waterbody size, ecoregion, and major river basin) categorical variables, and for individual sample sites. Sample site LS means were used to identify potential fish THg hotspots among lakes (LS means ≥ 90th percentile) and streams (Getis-Ord Gi* statistic). Across all samples (n = 2,106) a more than 200-fold difference existed between the highest (2.1 mg/kg) and lowest THg (0.01 mg/kg) concentrations, but most samples were below the Kansas Department of Health and Environment's (KDHE) human health screening level (HSL) (0.23 mg/kg). Statewide safe consumption limits ranged from 1 meal/month in ≥ 508 mm flathead catfish to 8 meals/mo. in crappie spp. Least squares mean fish THg was significantly higher in streams than lakes, and significantly higher in small lakes (< 10 surface acres) than larger lakes. Stream size did not affect fish THg levels. In both lakes and streams, ecoregion and major river basin significantly affected fish THg levels with higher levels more prevalent in eastern geographic areas. Lakes identified as potential fish THg hotspots were widely distributed throughout the study area, but all stream hotspot sites were clustered in the southeast portion of the state.

New Carnivoran (Mammalia: Carnivora) specimens from the Siwaliks of Pakistan and India and their faunal and evolutionary implications

ABSTRACT Recently collected carnivoran specimens from the Siwalik Group of Pakistan are described and discussed. These specimens add to our knowledge of the previously known taxa while also adding to our biogeographic, stratigraphic, and temporal knowledge of the carnivorans from the Siwaliks. At least eight distinct taxa are identified, and although most specimens are fragmentary, some hyaenid specimens are identified further (e.g. Lycyaena and Ictitherium). We identify and describe the first herpestid fossils from the Chinji Formation, the first hyaenid (Lycyaena cf. L. dubia) from the Tatrot Formation, and the first definitive occurrence of Ictitherium (Ictitherium cf. I. viverrinum) from the Dhok Pathan Formation. We report the first occurrences of several taxa from various sites in the Siwaliks of Pakistan, including the first reports of any carnivorans from Dhok Milan, Kohtehra, and Lawa. Individual sites show a wide range of carnivoran biodiversity, however larger scale trends are more discernible when comparing those between formations in the Siwaliks. Several taxa disappear over time from the carnivoran fauna of the Siwaliks, starting with barbourofelines, and continuing with amphicyonids (and non-carnivoran hyaenodontids). Felids and mustelids are the most diverse carnivorans in the Siwaliks, while caniforms become more prevalent through time.

RASCL: Rapid Assessment of Selection in CLades through molecular sequence analysis.

An important unmet need revealed by the COVID-19 pandemic is the near-real-time identification of potentially fitness-altering mutations within rapidly growing SARS-CoV-2 lineages. Although powerful molecular sequence analysis methods are available to detect and characterize patterns of natural selection within modestly sized gene-sequence datasets, the computational complexity of these methods and their sensitivity to sequencing errors render them effectively inapplicable in large-scale genomic surveillance contexts. Motivated by the need to analyze new lineage evolution in near-real time using large numbers of genomes, we developed the Rapid Assessment of Selection within CLades (RASCL) pipeline. RASCL applies state of the art phylogenetic comparative methods to evaluate selective processes acting at individual codon sites and across whole genes. RASCL is scalable and produces automatically updated regular lineage-specific selection analysis reports: even for lineages that include tens or hundreds of thousands of sampled genome sequences. Key to this performance is (i) generation of automatically subsampled high quality datasets of gene/ORF sequences drawn from a selected "query" viral lineage; (ii) contextualization of these query sequences in codon alignments that include high-quality "background" sequences representative of global SARS-CoV-2 diversity; and (iii) the extensive parallelization of a suite of computationally intensive selection analysis tests. Within hours of being deployed to analyze a novel rapidly growing lineage of interest, RASCL will begin yielding JavaScript Object Notation (JSON)-formatted reports that can be either imported into third-party analysis software or explored in standard web-browsers using the premade RASCL interactive data visualization dashboard. By enabling the rapid detection of genome sites evolving under different selective regimes, RASCL is well-suited for near-real-time monitoring of the population-level selective processes that will likely underlie the emergence of future variants of concern in measurably evolving pathogens with extensive genomic surveillance.

Al27 NMR insight into the phase transition in BaFe2Al9

We have applied $^{27}\mathrm{Al}$ nuclear magnetic resonance (NMR) spectroscopy to investigate the iron-based aluminide of $\mathrm{Ba}{\mathrm{Fe}}_{2}{\mathrm{Al}}_{9}$. This material has been a subject of current interest due to indications of charge density wave behavior below the transition temperature ${T}_{C}\ensuremath{\simeq}100$ K. Two sets of the $^{27}\mathrm{Al}$ NMR resonance lines that are associated with two nonequivalent crystallographic aluminum sites have been well resolved. We have discussed the obtained electric field gradient and anisotropic Knight shift for each individual aluminum site, revealing the strong $ab$ plane bonding configuration for the structural properties of $\mathrm{Ba}{\mathrm{Fe}}_{2}{\mathrm{Al}}_{9}$. Pronounced features in the isotropic Knight shift and nuclear spin-lattice relaxation rate have been observed in the vicinity of ${T}_{C}$. Furthermore, the detailed $^{27}\mathrm{Al}$ NMR analyses have provided evidence for the decrease of the electronic density of states upon lowering temperature below ${T}_{C}$.

Comparison of TROPOMI NO2, CO, HCHO, and SO2 data against ground‐level measurements in close proximity to large anthropogenic emission sources in the example of Ukraine

AbstractThe TROPOspheric Monitoring Instrument (TROPOMI) significantly improved the quality of air pollution monitoring. The comparison against other data sources showed acceptable results and good correlation. However, highly polluted areas remain the reason for large uncertainties. In our study, we compared TROPOMI NO2, CO, HCHO, and SO2 data against ground‐level measurements from Ukrainian monitoring sites, which could be considered rather unusual due to their close location to large anthropogenic emission sources. These sites were established in the former USSR to reflect the maximal air pollution levels. TROPOMI detected all cities with huge anthropogenic emissions in 2019–2020 and qualitatively reflected air pollution in Ukraine. However, direct comparison against individual monitoring sites showed mainly insignificant correlation, which was not much improved with cloudiness filtering, spatial averaging, and considering boundary layer height. We show that on an hour‐to‐daily scale, differences appear when remote sensing at a particular time cannot catch the elevated pollution levels in the boundary layer after air mass transportation. On a seasonal scale, the correlation decreases because of the differences in seasonality between near‐surface and column pollutants' content. We discuss that the relationship between TROPOMI and ground‐level measurements depends on the season, the continuance of industrial emissions, and the features of individual emission sources.

Replacing community clinical hours with an antepartum simulation-based experience.

Community and population health is an essential part of pre-licensure nursing education. Securing community clinical sites for large cohorts of nursing students is challenging. Individual community sites in nursing specialty areas, such as women's health, limit the number of students, making it difficult to provide a consistent clinical experience for all students. The literature shows simulation is an effective alternative to traditional clinical hours. This article describes a pilot simulation-based antepartum experience in the community setting. This simulation-based experience replaced the clinical hours for a previous traditional clinical experience in the community setting, for which students were only able to observe and not interact. The objectives were to allow students to be engaged in communication, patient education, and anticipatory guidance with a live patient. Faculty at a large southeastern university created an antepartum simulation-based clinical experience focused on important elements of obstetrics and maternal health assessment utilizing standardized patients. Student responses from the post-simulation survey showed 96% strongly agreed they were more confident in communicating with clients. Faculty concluded this simulation-based experience is an effective alternative to traditional community clinical experiences in the antepartum setting.

Mapping Middle Stone Age human mobility in the Makgadikgadi Pans (Botswana) through multi-site geochemical provenancing of silcrete artefacts

Analyses of the distance over which lithic raw materials were transported for use in stone tool production provide important insights into early human mobility through prehistoric landscapes. This study combines the use of geochemical provenancing, chaîne opératoire analysis and geochronology to examine patterns of lithic raw material procurement at five single-use open-air Middle Stone Age (MSA) archaeological sites in Ntwetwe Pan, part of the Makgadikgadi Pans complex in north-central Botswana. Maximum ages of the five sites ranged from 106 ± 3 to 69 ± 7 ka, with site formation thought to have occurred before and after a lake high stand dated to c. 72-57 ka. Tool manufacture at all sites was largely confined to the production of MSA points, with silcrete used exclusively as the raw material. Geochemical provenancing investigations aimed to identify the specific silcrete outcrops used as lithic raw material sources. Analysis of contemporary pan floor deposits show that sediment (and hence silcrete) geochemistry in Ntwetwe is determined by the proportional sediment input into the pan from fluvial systems with different catchment geologies. Immobile trace element signatures for 46 waste manufacturing flakes were compared against equivalent data for 321 silcrete samples collected at outcrops within and beyond Ntwetwe Pan. Waste flakes were chosen to be representative of the main silcrete raw material types present within the artefact assemblage at each site. Fifteen waste flakes were shown to match specific outcrops on the basis of their geochemical signatures and petrographic properties; all matching outcrops were within Ntwetwe Pan, at distances ranging from 7 to 55 km from individual sites. Multi-site analysis of procurement patterns identifies common silcrete source areas, revealing a preference for silcrete from particular locations within Ntwetwe Pan. Given that the five archaeological sites were likely occupied at different times, this resource preference may have been a longer-term behavioural feature of MSA populations in the Ntwetwe region. The distances over which silcrete was transported in Ntwetwe Pan are smaller than identified in investigations at similar-aged MSA sites in northwest Botswana. The reasons for the different silcrete procurement ranges in the two regions are likely related to silcrete availability and/or raw material preference, but this requires further investigation.