Individual Broiler Research Articles

RAPD PCR Type of Campylobacter Isolates from Cecum Contents of Broiles Chickens

In this investigation we typed Campylobacter isolates by RAPD PCR from the cecum contents of broiler chickens obtained at a slaughterhouse in Shizuoka prefecture. Campylobacter was isolated from 91 (56.9%) out of 160 birds from five out of eight flocks . Of the five Campylobacter positive flocks, three flocks possessed multiple RAPD types of Campylobacter, and two flocks possessed a single type. In the flocks where multiple types of Campylobacter were obtained, all the birds were positive and multiple RAPD types of Campylobacter were isolated from individual broiler samples. In these flocks, multiple episodes of Campylobacter contamination may have occurred . On the other hand, the three Campylobacter negative flocks were considered to have had no Campylobacter contaminations . The differences in Campylobacter colonization of the flocks in this study may, at least in part, reflect the differences in the hygiene measures of the broiler houses in which these birds are raised.

Effect of cage vs. floor litter environments on the pulmonary hypertensive response to intravenous endotoxin and on blood-gas values in broilers

Intravenous endotoxin has been shown to trigger a delayed pulmonary hypertensive response that varies widely in magnitude and duration among individual broilers. It was proposed that this individual variability may reflect immunological differences acquired during previous respiratory challenges that might have subsequently altered the endotoxin-initiated biochemical cascade. In Experiment 1, we tested the hypothesis that, when compared with broilers reared in clean stainless steel cages (Cage group), broilers reared on floor litter (Floor group) should experience a greater respiratory challenge and therefore may consistently exhibit a more enhanced pulmonary hypertensive response to intravenous endotoxin. Birds in the Cage group were grown in stainless steel cages at a low density (72 birds/8 m2 chamber), and fecal and dander materials were removed daily. Birds in the Floor group were reared on wood-shavings litter at a higher density (110 birds/8 m2 chamber). Pulmonary and systemic mean arterial pressures and blood-gas values were evaluated prior to and following the intravenous administration of 1 mg Salmonella typhimurium endotoxin. Broilers in the Floor and Cage groups exhibited pulmonary hypertensive responses to endotoxin that were very similar in terms of time of onset, duration, and magnitude, as well as variability in the response among individuals. Systemic hypotension also developed similarly in both groups following endotoxin injection. Blood-gas values indicated that the partial pressure of CO2 and the HCO3- concentration in arterial blood were higher (P < 0.05) in the Floor group than in the Cage group prior to and subsequent to the endotoxin injection. In Experiment 2, we reevaluated the effect of a dirty vs. a clean environment on blood-gas values using a different strain of broilers, and confirmed the negative impact of floor rearing on blood-gas values. We conclude that broilers reared on the floor inhaled litter dust and noxious fumes, which impaired pulmonary gas exchange and increased the arterial partial pressure of CO2 when compared with broilers reared in clean stainless steel cages. Nevertheless, the pulmonary hypertensive response to endotoxin did not differ between broilers reared on the floor and those in cages.

Pulmonary hypertensive response to endotoxin in cellulose-primed and unprimed broiler chickens

Previous studies indicate that individual broilers vary widely in their pulmonary vascular responsiveness to i.v. injections of endotoxin. This individual variability may reflect differences acquired during previous respiratory challenges or genetic variability that may be associated with susceptibility to pulmonary hypertension syndrome (ascites). In the present study, we compared the endotoxin responses of 4- to 5- wk-old control broilers (unprimed) and broilers in which the pulmonary vasculature had been immunologically challenged 48 h previously by an i.v. injection of cellulose micro-particles (primed). The injected cellulose micro-particles are carried in the venous blood to the lungs, where they become trapped in the pulmonary vasculature and initiate acute focal inflammatory responses within the surrounding lung parenchyma. Physiological variables (respiratory rate, heart rate, pulmonary and systemic arterial pressures) were evaluated prior to and following the i.v. administration of 1 mg of Salmonella typhimurium endotoxin. Prior to endotoxin injection, the respiratory rate was higher in primed than in unprimed broilers; however, the heart rate, pulmonary arterial pressure, and systemic arterial pressure did not differ between groups. Broilers in both groups exhibited similar ranges of individual variability in their endotoxin responses. The overall time of onset, magnitude, and duration of the pulmonary hypertensive responses were similar for both groups. Accordingly, the initiation of a preexisting inflammatory response within the lung parenchyma did not alter the timing, amplitude, or variability of the subsequent pulmonary hypertensive response to endotoxin in broilers.

Changes in the carriage of Campylobacter strains by poultry carcasses during processing in abattoirs.

The recent development of simple, rapid genotyping techniques for Campylobacter species has enabled investigation of the determinative epidemiology of these organisms in a variety of situations. In this study we have used the technique of fla typing (PCR-restriction fragment length polymorphism analysis of the flaA and flaB genes) to identify the sources of strains contaminating the carcasses of five campylobacter-positive and two campylobacter-negative broiler flocks during abattoir processing. The results confirmed that, in the United Kingdom, individual broiler flocks are colonized by a limited number of subtypes of Campylobacter jejuni or C. coli. In some but not all cases, the same subtypes, isolated from the ceca, contaminated the end product as observed in carcass washes. However, the culture methodology, i.e, use of direct plating or enrichment, affected this subtype distribution. Moreover, the number of isolates analyzed per sample was limited. fla typing also indicated that some campylobacter subtypes survive poultry processing better than others. The extent of resistance to the environmental stresses during processing varied between strains. The more robust subtypes appeared to contaminate the abattoir environment, surviving through carcass chilling, and even carrying over onto subsequent flocks. From these studies it is confirmed that some campylobacter-negative flocks reach the abattoir but the carcasses from such flocks are rapidly contaminated by various campylobacter subtypes during processing. However, only some of these contaminating subtypes appeared to survive processing. The sources of this contamination are not clear, but in both negative flocks, campylobacters of the same subtypes as those recovered from the carcasses were isolated from the crates used to transport the birds. In one case, this crate contamination was shown to be present before the birds were loaded.

Intravenous Endotoxin Triggers Pulmonary Vasoconstriction and Pulmonary Hypertension in Broiler Chickens

Bacterial endotoxins stimulate endothelin-mediated, thromboxane-dependent increases in pulmonary vascular resistance in mammals, and thromboxane has been shown to cause an immediate but transient pulmonary vasoconstriction in broiler chickens. In the present study, i.v. injections of 1 mg endotoxin into anesthetized male broilers caused a pulmonary vasoconstrictive response that was delayed in onset by 15 min and that elevated the pulmonary arterial pressure by 10 mm Hg within 25 min postinjection. Thereafter, pulmonary hemodynamic variables gradually (≥15 min) returned toward pre-injection levels, and supplemental injections of 4 mg endotoxin during this recovery period failed to reinitiate pulmonary hypertension. In contrast, injecting the thromboxane A2 mimetic U44069 during the endotoxin recovery period triggered pulmonary vasoconstriction and pulmonary hypertension similar in magnitude to the responses triggered by U44069 before endotoxin had been administered. The time course and magnitude of the pulmonary hemodynamic responses to endotoxin were highly variable among individual broilers, whereas the individual responses to U44069 were more consistent. Unanesthetized broilers resembled anesthetized broilers in the time course, magnitude, and variability of their pulmonary hemodynamic responses to endotoxin. Overall, these observations are consistent with the hypothesis that endotoxin initiates a biochemical cascade, culminating in the delayed onset of pulmonary vasoconstriction and pulmonary hypertension within 20 min postinjection. Subsequently, the pulmonary vasculature remains responsive to large bolus injections of exogenous thromboxane mimetic; however depletion of endogenous vasoconstrictive components of the endotoxin-mediated cascade, a compensatory increase in endogenous vasodilators, or the induction of a transient cellular tolerance to endotoxin prevented fourfold higher doses of endotoxin from reversing the return toward a normal pulmonary vascular tone. Individual differences among broilers in their susceptibility to pulmonary hypertension syndrome (ascites) may be related to innate or acquired variability in their pulmonary vascular responsiveness to vasoactive mediators.

Risk factors associated with Salmonella enterica serovar typhimurium infection in Danish broiler flocks

A retrospective longitudinal study was conducted to identify risk factors associated with Salmonella enterica serovar typhimurium (S. typhimurium) infection in Danish broiler flocks. The data included all broiler flocks slaughtered in 1995, and the epidemiological unit was the individual broiler flock. The S. typhimurium status was determined by microbiological examination of 60 fresh fecal samples. This procedure should detect an infected flock with a probability above 95%, if the prevalence is above 5%, and given that the sensitivity of the test is 100%. Nineteen variables were selected for analysis. Five factors and an interaction term were found significant by multivariate logistic regression analysis. An increased risk for S. typhimurium infection was associated with two parent flocks, one confirmed infected and one suspected of being infected with S. typhimurium, with two of the hatcheries, and with five houses on the farm. An interaction between season and the previously mentioned hatcheries, and a random effect at farm level was also found to be statistically significant. Twelve variables were not found to be associated with S. typhimurium infection: medication, growth promoters, breed of the laying flock, animal density, size of the flock, area of the house, age of the house, geographical location of the farm, observation of beetles, number of days between disinfection and replacement, visual appearance of the bedding, and age of the chickens when they were tested for Salmonella. Three variables (feed mill, slaughterhouse, and Salmonella status of the preceding flock) were not evaluated in the multivariate analysis due to collinearity with other included variables.

Evaluation of Salmonella Serotype Distributions from Commercial Broiler Hatcheries and Grower Houses

By conventional trayliner (hatcheries) and drag swab assembly (broiler houses) culture methods, the isolation distribution of Salmonella serotypes from five commercial broiler hatcheries (three sample times) and 13 broiler farms (eight sample times) was evaluated. A total of 11 different Salmonella serotypes were isolated from hatcheries, with Salmonella heidelberg (9/30) and Salmonella kentucky (6/30) accounting for 50% of the total isolations. Of 700 chick paperpad trayliners sampled, regardless of lot (breeder flock source) or hatchery, 12% were positive for Salmonella. When 10 individual trayliners were cultured from individual lots (same breeder flock source), Salmonella was detected in 24/57 lots (42%). Multiple serotypes were simultaneously isolated from the same lot on three occasions (6%). Of the 21 lots that were serially sampled, the Salmonella serotype detected was different within lots eight times (38%) on at least one occasion of two or more sampling times. Of the 196 individual broiler houses sampled, 44 were positive for Salmonella (42%). Twelve different serotypes were isolated from broiler houses during this study. The serotypes isolated most frequently were S. heidelberg (34/94) and S. kentucky (22/94). These two serotypes accounted for 59.6% (56/94) of the total broiler house isolations. Of the 38 houses that were serially sampled, two or more serotypes were detected in the same broiler house on 20 occasions (53%). Of the 38 serially sampled houses (four or more times), a consistent Salmonella serotype was detected in five houses (13%). In only 5 of the 38 (13%) serially sampled houses did we fail to detect Salmonella on four or more samplings. No significant difference in Salmonella isolation frequency was observed between poultry houses using new or used litter. These data support previous findings indicating that paratyphoid Salmonella serotypes are prevalent in some broiler hatcheries and houses. Further, the observation of multiple serotypes simultaneously and serially isolated from the same breeder hatchery lots suggests that breeder flocks may be infected with more than one serotype, possibly providing a source for multiple serotype infections in progeny grower flocks.

Frequency of Isolation of Salmonella from Protective Foot Covers Worn in Broiler Houses as Compared to Drag-Swab Sampling

In this study we tested the hypothesis that the frequency of Salmonella isolation from protective foot covers worn in individual broiler production houses would compare favorably to isolation rates obtained from conventional drag-swab methods. Salmonella was detected with equal frequency from protective foot covers and drag-swab assemblies on nine individual broiler farms over three separate sampling periods. Salmonella was detected in 13 of a total 27 individual samplings by culturing the protective foot covers, whereas positive detections occurred in 16 of a total 27 samplings when using the drag-swab method. Of the total number of houses identified as positive, these frequencies were unaltered when evaluated against the flock status of each farm at the time of sampling. Both methods were equally as likely to detect Salmonella when houses were either vacant (awaiting the placement of the next flock) or occupied (currently housing chickens of any age). In addition to highlighting the development of a potential new Salmonella monitoring technique, this study reinforces our current understanding regarding the importance of stringent biosecurity practices on poultry farms.

Chronic unilateral occlusion of an extrapulmonary primary bronchus induces pulmonary hypertension syndrome (ascites) in male and female broilers

Previously, it was demonstrated that acute (4 min) and chronic (12 d) occlusion of an extrapulmonary primary bronchus triggers pulmonary hypertension but not pulmonary hypertension syndrome (PHS, ascites) in broilers. The present study was conducted to determine whether a more prolonged period of bronchus occlusion causes PHS similar to that induced by clamping one pulmonary artery. Male and female broiler chicks, 14 to 18 d old, were anesthetized, the thoracic inlet was opened, and a silver clip was positioned to fully obstruct the left extrapulmonary primary bronchus (BRONCHUS CLAMP group) or the left pulmonary artery (PA-CLAMP group). Sham-operated chicks were anesthetized and the thoracic inlet was opened; however, neither the pulmonary artery nor the bronchus was clamped (SHAM group). An electrocardiogram (ECG) was obtained whenever clinical ascites became apparent in individual broilers, or prior to the final necropsy for broilers surviving to the end (Day 36) of the experiment. The right:total ventricular weight ratio (RV:TV) was evaluated as an index of pulmonary arterial pressure. Early post-surgical mortality (up to 21 d of age) was higher in the PA-CLAMP group (27% for males and females combined) than in the BRONCHUS CLAMP (10%) and SHAM (2%) groups. Cumulative ascites mortality (Days 22 to 36) also was higher in the PA-CLAMP group (86% for males, 77% for females) than in the BRONCHUS CLAMP (69% for males, 41% for females) and SHAM (23% for males, 0% for females) groups. Ascitic birds in all treatment groups had higher RV:TV ratios and more negative ECG Lead II S-wave amplitudes than nonascitic birds, reflecting the right ventricular hypertrophy and generalized ventricular dilation typically associated with PHS. These results demonstrate that unilateral bronchus occlusion is an effective experimental model for triggering ascites at a lower incidence than that obtained by occluding one pulmonary artery. Following the onset of pulmonary hypertension, the pathophysiological progression leading to ascites appears to be similar for broilers with either unilateral bronchus or pulmonary artery occlusion.

A retrospective study on salmonella infection in Danish broiler flocks

A retrospective longitudinal study was conducted to identify risk factors associated with Salmonella enterica infection in Danish broiler production. The study was based on information in the antemortem database (AM database) where data were available for all broiler flocks slaughtered over the 2-year period from 1992 to 1993 in Denmark. The AM database contains information collected by the ante-mortem veterinarians, from the slaughterhouses, and from the salmonella examinations carried out at the National Veterinary Laboratory. The epidemiological unit was the individual broiler flock. The salmonella status of the flock was determined by examining the caecal tonsils from 16 3-week-old chickens from each flock. This procedure would detect a salmonella-infected flock, with a probability above 95%, if the prevalence is above 20%. Furthermore, the structure and quality of the collected data have been evaluated. Fourteen variables were selected for analysis by multivariable logistic regression. An increased risk of salmonella infection in the broiler flocks was associated with the biggest hatcheries and feedmill, with an increasing number of houses on the farm, if the preceding flock was infected, and if the flock was reared in the autumn. Additionally, the main variables of the model were analysed by including a random effect at the house level. This resulted only in minor changes of the parameter estimates.

Relationship between the weight of hatching eggs and subsequent early performance of broiler chicks

1. The effect of egg weight on the subsequent performance of individual growing chicks was studied. Eggs from commercial broiler breeder hens were collected at 52, 55 and 57 weeks of age and incubated. The chicks were reared for 18 d. 2. Egg weight and hatched chick weight were not affected by the age of the hens during the 52 to 57-week period, but there was a close correlation between egg weight and hatching weight, irrespective of the age of the hens (r = 0.89). Chick:egg weight ratio at hatching was about 0.71 and independent of hen age. One d after hatching, chick weights had decreased by about 1.5 g. 3. The initial high correlation between egg weight and chick weight decreased markedly during post-hatching growth, becoming insignificant 5 d after hatching. At 18 d of age, however, chicks from eggs of older hens tended to be heavier than those from younger birds. The greater 18-d weights appeared to be associated more with higher food intake during that period, than with greater egg weight. 4. It was concluded that, in individual broiler chicks, the advantage of the initially higher weight attributable to a larger egg diminishes rapidly after hatching, and the main factor affecting final body weight is food intake.

Research Note: The Use of Carcass Halves for Reducing the Variability in Salmonellae Numbers with Broiler-Processing Trials

Two similar trials were conducted in order to compare the variability in the number of salmonellae between the right and left sides of individual broiler carcasses with the variability among carcasses. In both trials, the variation between carcass sides was equal and was significantly less than the variation among carcasses. For trials involving bactericidal treatment, the authors would suggest that the utilization of carcass halves (one side for control, the other for treatment) would allow for a more-sensitive evaluation of treatment effects.

Age-Constant Versus Weight-Constant Feed Consumption and Efficiency in Broiler Chickens

Weekly body weights, feed consumptions (FC), and feed efficiencies (FE = gain:feed) of 148 male and 125 female broilers were measured individually between 2 and 7 wk of age. Multiple regression analyses of these traits were performed to examine the influence of body weight, weight gain or feed consumption, age, and individual broiler differences. Four different measures of FC and FE from 4 to 6 wk of age were compared within sex: age-constant FC and FE; weight-constant FC and FE; age-constant-weight-corrected FC and FE; and weight-corrected FC and FE, which estimate weight-constant measures.Much of the variation (85 to 90%) among broilers in FC and FE was due to age and body weight differences. Partial regression coefficients indicated that increased body weight at commencement of testing was associated with increased FC and reduced weight gain and FE. Correlations between age-constant and weight-constant FC were zero. This indicates the two forms have little in common. Correlations of body weights and weight gain with FC were negative (−.5 to −.8) for weight-constant values but positive (.5 to .8) for age-constant values. Hence, correlations of the correct (weight-constant) measure of FC with body weights indicate that faster growth gives rise to lower broiler feed consumption. Age-constant and weight-constant FE values were positively correlated (.8 to .9); however, the latter values had larger correlations with body weights and weight gain. These results emphasize the need to correct age-constant FC and FE for body weight differences in order to evaluate differences in efficiency among broilers more accurately.

A computerised management and disease information retrieval system for profitable broiler production

The effective management of a large poultry organisation necessitates the recording and analysis of all major production parameters which affect the performance of individual broiler flocks. No comprehensive computerised recording system has been established for the monitoring and control of optimal broiler production. This paper describes a computerised information and retrieval system for broiler production data. The problems associated with data collection and integrity from many, widely-distributed locations are highlighted and a solution, utilising a centralised system, detailed. Initial results from the analysis of data from 1557 flocks incorporating 20 036 579 birds are reported. The presence of both poor litter conditions and disease in flocks is shown to have a severe detrimental effect on profitability. The major effect that seasonality has on net income and the incidence of an economically important dermatitis lesion is also recorded. The system is being used by the industry both to monitor and optimise many major broiler production parameters.

Drinking behaviour of broiler chicks

A study of drinking behaviour of individual broiler breeder chicks from hatching to 5 weeks of age was conducted. The action patterns involved in obtaining a drink as well as water consumption were described quantitatively and qualitatively. Over the experimental period, the action patterns of drinking were modified gradually. Marked changes in immersion angle, swallowing angle and distance from the waterer were evident. All birds made progressively fewer trips to the waterer to take a larger number of higher volume drinks. This information is essential for future design of improved watering equipment to better complement the natural drinking behaviour of broiler chickens.

Bird to Bird Variation Within Processing Lots for Mineral Elements, Proximate Constituents, and Part Weight in Broilers

Breast and thigh meat samples from 40 individual broilers from a single commercial processing source were analyzed for calcium, copper, iron, phosphorus, magnesium, sodium, and zinc by atomic absorption spectrophotometry. Phosphorus was determined spectrophotometrically as the antimony-phosphate-molydbate complex. Assays of breast meat ranged from 25% higher than previously reported for potassium (3190 vs. 2550 ppm, wet weight basis) to 25% lower for sodium (485 vs. 650). Breast and thigh meat samples differed in the concentration of all elements checked. The differences were not due exclusively to dilution of sample by a higher fat level in thigh meat, since on a wet weight basis sodium, calcium, zinc, iron, and copper were higher in thigh than in breast meat.

The Influence of Fasting and Transport on Yields of Broilers

Yields of the components of carcasses were measured on broilers fasted different periods of time prior to slaughtering.The total wt losses after 4 hr of fasting are .353%/hr. These losses are mainly caused by the losses of the edible parts, which are .24%/hr.The results of measuring the losses of dry solids, water, and fat of the edible parts without giblets are hard to interpret due to the great variation in the chemical composition of the edible parts of individual broilers.

Effects of temperature treatments on the energy and nitrogen metabolism of fed chickens.

1. Calorimetric measurements were made for 5 d on individual broiler chickens (1 kg) fed ad libitum and acclimated or unacclimated to six temperatures from 2 to 35 °C (constant), or alternated between these temperatures and 22 °G (alternated). For the four treatments, heat production was related to temperatures and the resultant curves were significantly different. 2. Food intake, heat production and maintenance energy requirement all increased linearly with decreasing temperature. Metabolisability of the diet was only 13.8 kJ/g at 35 °C, compared with a mean value for all treatments of 14.1 kJ/g. 3. Energy retention and nitrogen (N) retention (g/d, or % dietary N) were maximal at 22 and 16 °C, however the amount of energy deposited as protein remained relatively constant below 30 °C. 4. Net availability of metabolisable energy was calculated in two ways: by calculating the increase in heat production of fed birds above their starvation values giving a mean value of 0.82; this was similar to the mean regression coefficient which included starvation data, and related ME intake and energy retention; but without these data availabilities ranged from 0.45 at 35 °C to 1.0 in the cold. 5. Acclimation or alternating of temperature had very few significant effects, however there were temperature x acclimation effects on N retention and heat production. Similarly alternating temperature significantly increased food intake and heat production at high temperatures, but decreased metabolisability of the diet to 13.7 kJ/g at 35 °C from an overall mean of 14.1 kJ/g.

Cages and Feed Troughs for Individual Broiler Feed Consumption Experiments

A system for measuring individual broiler feed consumption of chickens is described. The system consisted of modified Chucker quail breeding cages and feeders constructed from milk cartons. The system is inexpensive, ($6.34/bird) has virtually no feed wastage, and requires minimal labor.