Indiscriminate Use Of Pesticides Research Articles

Assessment of pesticide knowledge, Safety Practices and postharvest handling among cocoa farmers in South Western Nigeria

Cocoa beans are susceptible to fungal contamination during processing and storage. The knowledge of the use of pesticides and post-harvest handling of cocoa beans among farmers is of great importance for safe consumption. The study evaluated common cocoa production and post-harvest practices of farmers in selected study locations in South Western Nigeria. Primary data were collected through the administration of structured questionnaires, and interviews. The collected data were analyzed using inferential descriptive statistics. The results of 394 farmers showed that 52.9 % in Osun and 47.3 % in Oyo were primarily farmers by occupation, the rest had other ventures. The majority of cocoa farmers were men:83.6 % in Oyo State, 88.2 % in Osun state and 87.9 % in Ondo state. 28.6 % and 32.7 % of farmers were aged 51–60 in the Ondo and Oyo communities, respectively. Osun farming communities are dominated by young adults (51 %) of 31–50 years, followed by Ondo 40 % and36 % of farmers in Oyo State. Most cocoa farmers were married with 4–6 children as the most common household size in Osun (51 %), Ondo (60.4 %) and 49.1 % in Oyo State. The literacy level of farmers in cocoa communities was the highest in Oyo state where 47.3 % had tertiary education. Farmers in Oyo State had better knowledge of the dangers of pesticides than Ondo and Osun. However, ignorance of dangers in agrochemicals was higher among Osun farmers than in Ondo State. The highest (18 %) pesticide use during storage was recorded among Oyo farmers, while the least (11.0 %) was recorded among farmers in Ondo State. Pesticide usage was more abundant in Osun (50 %) during cocoa production than in the other study areas. The majority of farmers were positively disposed to make use of nose masks during agrochemical application, meanwhile, 69 %, 62 %, and 61 % of farmers used them already in Oyo, Ondo, and Osun states, respectively. Educational qualification (χ2 = 9.176, p = 0.027) of cocoa farmers was significantly related to knowledge of best practices. Farmers with higher education have a greater ability to receive and process information relating to global best practices in production, postharvest, and pesticide handling in cocoa. In conclusion, cocoa farmers’ knowledge of processing, use of pesticides, and storage practices differed from one location to another. Intensive orientation and more enlightenment by extension workers against indiscriminate use of pesticides in cocoa plantations and stores must be consistently and continuously done.

The Indiscriminate Use of Pesticides could Increase the Prevalence of Alzheimer's Disease? A Systematic Review

Aims: This review aims to perform an extensive literature search about the pesticides problems and to associate with AD in qualitative analysis, mainly. Place and Duration of Study: Biomathematics Laboratory, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Brazil. Entre outubro de 2023 a abril de 2024. Methodology: For the purpose of this systematic review, articles from 2014 onwards with the descriptors Alzheimer's disease and pesticides; neurodegenerative diseases and pesticides, were sought. Among these, articles considered most pertinent to the objective of the present review were utilized, i.e., those whose subject matter was associated with the effects of pesticides, especially glyphosate, on dementias, particularly Alzheimer’s disease (AD). Results: After analyzed 35,590 articles, and applying exclusion criteria to journals with an impact factor equal to or lower than 4 and including topics most relevant to the objectives of this work, 35,526 articles were excluded, resulting in 64 remaining articles, of which 40 were qualitative in scope and 24 were quantitative. The articles considered most suitable for the objective of the present review were utilized, i.e., those whose topic was associated with the effects of pesticides, especially glyphosate, on dementia. The criteria for scrutinizing articles included a journal impact factor equal to or greater than 4 and the removal of duplicate articles using the freely accessible EndNote program from Web of Science. Articles and books on history and those outside the scope of the pesticide/AD relationship did not follow the criterion of having an impact factor equal to or greater than 4. Conclusion: There appears to be a relationship between the increase in pesticide use, particularly Glyphosate, and the rise in Alzheimer's disease prevalence.

Organophosphate pesticide residue impact on water quality and changes in macroinvertebrate community in an Afrotropical stream flowing through farmlands.

River Chanchaga has experienced significant agricultural practices around its catchment, which involved the indiscriminate use of pesticides. However, residents of the study area are not well aware of the negative impact of pesticides on water quality and macroinvertebrates. In this study, the first report on the influence of organophosphate pesticide contamination on the abundance of the macroinvertebrate community was provided. Sampling for the determination of organophosphate pesticide residues was carried out during the peak of the two seasons, while macroinvertebrates and physicochemical variables were observed for 6 months. We examined 11 organophosphate pesticide residues using gas chromatography coupled with mass spectrometry, 12 water quality variables, and 625 macroinvertebrate individuals. The concentration of recorded organophosphate pesticide residues ranged from 0.01 to 0.52 μg/L. From the Canonical Correspondence Analysis plot, malathion, chlorine, and paraffin show a positive correlation with Unima sp., Hydrocanthus sp., Chironomus sp., and Potadoma sp. At station 3, depth shows a positive correlation with Biomphalaria sp. and Zyxomma sp., indicating poor water quality as most of these macroinvertebrates are indicators of water pollution. Diuron and carbofuran show a negative correlation with Lestes sp. and Pseudocloeon sp., and these are pollution-sensitive macroinvertebrates. The total mean concentration of organophosphate pesticide residues was above international drinking water standards set by the World Health Organization except for paraffin, chlorpyrifos, and diuron. In conclusion, the observations recorded from this research are useful in managing pesticide applications around the river catchment.

INDUCED TOXIC EFFECTS OF CHLORPYRIFOS ON HEMATOLOGICAL INDICES IN PIGEONS (COLUMBA LIVIA DOMESTICA)

Agrochemicals are used extensively in agriculture sector across the world; however, they may be eco-toxicological hazards and disrupt the non-target living beings, including birds. Hence, the present study was carried out to evaluate the toxic effects of chlorpyrifos (CPF) insecticide on hematological parameters in pigeons. A total of 120 adult pigeons (Columba livia domestica) were used in the study and kept in wooden cages, and all pigeons received the same quantity of (22%) crude protein and water during trials. Pigeons were acclimatized for 15 days and divided into four equal (A, B, C and D) groups, each consisting of 30 birds. Test groups (B-D) were exposed to CPF with oral doses of “1.3 mg, 1.6 mg, and 2.1 mg/kg body weight/day” respectively for twelve successive weeks, whereas (group A) was assigned as a controlled group. At the end of the trials, blood samples from exposed and controlled group birds were obtained from brachial and collected with anticoagulant “K3-EDTA” vacutainer tubes for hematological assessments. Following the 84-day exposure period, notable statistically significant (p < 0.05) reduced levels of hemoglobin (Hb) (13.85 g/dl), (12.2 g/dl) and (10.50 g/dl) respectively, in birds of groups B, C and D and significant (p < 0.05) reduced level of total erythrocytes count (TEC) and packed cell volume (PCV%) was noted when compared with the control. There was a substantial significant (p < 0.05) increase in total leucocyte count (TLC) and total platelet count observed in comparison to the birds of the control group. The results of the present study revealed that alterations in hematological indices exhibited by chlorpyrifos insecticide. This research demonstrated how the indiscriminate use of pesticides damages health and developmental problems in bird ecosystems, and eventually, humans may be in danger for their health problems

Bioremediation Studies on Pesticides in Soil by Novel Microorganisms Isolated from Agricultural Fields

Pesticides are organic compounds widely used in agriculture for increasing agricultural yield, soil productivity and to control the insect vectors for prevention of various plants, human and animal epidemics. Pesticides are widely distributed in our ecosystem. Organophosphates are found to be one of the most common group of pesticides found across the world. However, excessive and indiscriminate use of pesticides have generated a number of environmental problems such as release of toxic waste in the environment resulting to contamination of air, water and terrestrial ecosystem. In order to control the harmful effects of pesticides, multiple attempts have been made like the use of physical, chemical, biological and enzymatic approaches to reduce pesticides. However, physical and chemical methods have proven to be inefficient. One of the most promising field of biotechnology that offers a novel approach to degrade the pesticides is bioremediation. Bioremediation uses microorganisms, microbial or plants enzymes to detoxify contaminants in the soil and other environments. Three group of microorganisms i.e., bacteria, actinomycetes and fungi have been found to degrade the pesticides. Bacterial group involved in degradation includes Bacillus, Pseudomonas, Flavobacterium, Arthrobacter, Paracoccus, Aerobacter, Alcaligens, and Sphingomonas. Fungi having potential bioremediation capacities includes Fusarium, Aspergilus niger, Penicillium, Oxysporum. Streptomycetes have been found to degrade pesticides among the actinomycetes group. This review highlights various aspects of microbial degradation of pesticides including the types of pesticide degrading microbes and mechanism of degradation.

Farmers Perspectives on Negative Impacts of Chemical Pesticide Usage in Vegetable Cultivations: Case Study from the Periyaneelavanai Area, Ampara District

Indiscriminate use of pesticides causes health hazards to humans and long-lasting negative impacts to the environment. The major objectives of this study are to evaluate the current status of pesticide usage in the Periyaneelavani area, to evaluate farmers‘ perception on health, environmental effects due to usage, of pesticides, to identify the awareness level of farmers about proper usage of chemical pesticides and alternatives. This study was carried out in two GN divisions of Periyaneelavanai area, Ampara district. To represent 20% respondents, 50 farmers from each GN devisions which upto total 100 were randomly surveyed using a structured questionnaire. In order to gather the necessary data on vegetable cultivation, a preliminary study involving interviews with the Agricultural Instructor-Officer in Charge and affiliated farmers in Periyaneelavanai area were conducted before the actual survey. Discriptive analysis, P test, T test, anova were performed using minitab 14.0 version to conduct data analysis. The present study revealed that Chili, Brinjal, Okra, Tomato, Long beans, Cucurbites, Onion and Leafy vegetables were primarily grown in this area. Abamactin, Coragan, Applaud and Virtako were used as insecticides and Abamactin was the most popular (42%) among farmers. Mancozeb, Propineb and Canazole were used as fungicides and the highest usage was Mancozeb (45%). Mortin rat kill was used as a rodenticide by all the respondent farmers, and they have not used herbicides for their vegetable cultivation; instead, they manually remove the weeds. Skin irritation was the most prominent (60%), acute disease, and Asthma was the most common chronic disease (31%), that can be seen among farmers. The most common adverse environmental impact in this area was the empty pesticide containers piling up along roadsides (95%). All the farmers use at least one PPE while applying pesticide though the frequency of wearing PPE is different among each other. The majority of respondents use gloves, eye protection, and foot ware but lack of awareness towards face, head and body protection. The highest number of farmers selected indoor places (51%) for pesticide storage which cause to have a high risk of exposure among farmers. Most of the farmers selected the option of burying or burning pesticide bottles after using it. Majority of respondents preferred to use chemical pesticides (76%), rather than alternative methods due to the reliability of those methods. It is proposed to educate farmers on application, storage, cleaning and carrying the pesticides, the methods of effectiveness of different individual protection facilities, and utilizing the behavior to reduce the exposure to poisons should be developed and be available to farmers. This will help to reduce negative health and environmental consequences. Moreover, it is recommended to introduce reliable and effective alternative methods for chemical pesticides.
 Keywords: Periyaneelavanai area, Vegetable cultivation, Pesticides, Environmental impacts, Disease

Flowering margins support natural enemies between cropping seasons

IntroductionPopulations of natural enemies of insect pests are declining owing to agricultural intensification and indiscriminate use of pesticides, and this may be exacerbated in agricultural systems that clear all margin plants after the cropping season for other uses such as fodder. Retaining a diversity of non-crop flowering vegetation outside the cropping season may support more resilient and effective natural pest regulation.MethodsWe tested the potential for non-crop vegetation to support natural enemies in fields across two locations after harvesting the primary crops of lablab and maize.ResultsA total of 54 plant species were recorded across the sites in Kenya with 59% of them being annuals and 41% perennials. There was a significant seasonal variation in plant species richness (ANOVA: F1, 16 = 33. 45; P< 0.0001) and diversity (ANOVA: F1, 16 = 7.20; P = 0.0511). While time since harvesting was a significant factor influencing the overall abundance of natural enemies (ANOVA: F2, 1,133 = 8.11; P< 0.0001), they were generally higher in abundance in locations with margin plants or where a diversity of margin plants was observed.DiscussionThese findings demonstrate that flowering plants in agricultural systems offer refuge and alternative food for natural enemies and potentially other beneficial insects between cropping seasons. The conservation of natural enemies between crops may lead to more effective natural pest regulation early in the following crop, thus reducing reliance on insecticides application.

Balancing food security and environmental safety: rethinking modern agricultural practices

Agriculture has played an important role in human life, both for sustaining life and livelihood. The population explosion has necessitated huge agricultural production. Consequently, there has been modernisation of agriculture not only in farming practices, but also in introducing improved agricultural implements, irrigation, chemical fertilisers, synthetic pesticides, and high-yielding seeds. Agricultural intensification and monoculture make it possible to increase crop production, to a large extent gaining food security, but paying no or little attention to environmental well-being. Intensive tillage leads to soil erosion, nutrient loss, and soil organic carbon loss, which affects the soil biota. Extraction of underground water for irrigation causes groundwater levels to drop and hinders aquifer recharge. Monoculture and the cultivation of high-yielding crops lead to the loss of many indigenous crop varieties and the prevalence of pests and pathogens. Extensive chemical fertiliser application can cause soil acidification, eutrophication, and nitrate contamination in groundwater through leaching. Indiscriminate use of pesticides is a potential threat for non-target organisms, including humans. The agriculture sector contributes a considerable portion of greenhouse gases to the atmosphere. Therefore, the only way to protect our mother earth and create a healthy environment is through sustainable agriculture to ensure food safety and security.

Is Anopheles gambiae (sensu stricto), the principal malaria vector in Africa prone to resistance development against new insecticides? Outcomes from laboratory exposure of An. gambiae (s.s.) to sub-lethal concentrations of chlorfenapyr and clothianidin

Indiscriminate use of pesticides in the public health and agriculture sectors has contributed to the development of resistance in malaria vectors following exposure to sub-lethal concentrations. To preserve the efficacy of vector control tools and prevent resistance from spreading, early resistance detection is urgently needed to inform management strategies. The introduction of new insecticides for controlling malaria vectors such as clothianidin and chlorfenapyr requires research to identify early markers of resistance which could be used in routine surveillance. This study investigated phenotypic resistance of Anopheles gambiae (sensu stricto) Muleba-Kis strain using both WHO bottle and tube assays following chlorfenapyr, clothianidin, and alpha-cypermethrin selection against larvae and adults under laboratory conditions. High mortality rates were recorded for both chlorfenapyr-selected mosquitoes that were consistently maintained for 10 generations (24-h mortality of 92–100% and 72-h mortality of 98–100% for selected larvae; and 24-h mortality of 95–100% and 72-h mortality of 98–100% for selected adults). Selection with clothianidin at larval and adult stages showed a wide range of mortality (18–91%) compared to unselected progeny where mortality was approximately 99%. On the contrary, mosquitoes selected with alpha-cypermethrin from the adult selection maintained low mortality (28% at Generation 2 and 23% at Generation 4) against discrimination concentration compared to unselected progeny where average mortality was 51%. The observed resistance in the clothianidin-selected mosquitoes needs further investigation to determine the underlying resistance mechanism against this insecticide class. Additionally, further investigation is recommended to develop molecular markers for observed clothianidin phenotypic resistance.

A Comparison of Maximum Permissible Concentrations for Pesticides in Brazilian Water Supply

Abstract Pesticides have been used for a long time in agriculture to prevent the growth of undesired organisms. However, depending on the type of production (i.e., conventional, organic, and regenerative), they might not be applied. Adequate types and uses of pesticides should not cause any damage to any non-target species or environments due to the exposure to it, especially if all farming operations have been done properly. Reversely, when pesticides are applied in inappropriate time, amount or technique, they may cause several impacts, among which is water contamination. Brazil stands out worldwide for its agricultural potential, but it is exposed to risks concerning the water supply security to its population. The current study is a comparison of the Maximum Permissible Concentrations (MPC) for pesticides in potable water supply in Brazil with the values set in the United States, Canada, European Union countries, Japan, and the guidelines issued by the World Health Organization. The regulations of each country were used, as well as the trading, consumption and agricultural production data. The Brazilian regulation is the one accounting for the largest number of pesticides. However, their MPC is 5,000 times more permissible than that from the European Union for the herbicide known as glyphosate, 300 times than that for 2,4-d and 20 times than that for atrazine. Finally, it was possible to observe the relevance of revising the regulation and public policies in place to minimize the indiscriminate use of pesticides in Brazil and adapt these compounds to the standards in a global level.

Infectious Diseases Associated with Exposure to Pollutants in a Local Population from Mexico

Agriculture is one of the primary activities in the municipality of Acatzingo, Puebla, Mexico. Agricultural fields are affected by the indiscriminate use of pesticides and hydrocarbon spills, which expose inhabitants to the development of infectious diseases. In the present study, we assessed the likelihood of developing infectious diseases associated with environmental contamination (pesticides and hydrocarbons) in various areas of the municipality of Acatzingo, Puebla, Mexico. A questionnaire was applied to 425 people in four areas of two locations divided according to exposure to hydrocarbons and pesticides. We conducted a binomial analysis using a binary logistic regression model, and the odds ratio (OR) was calculated at p ≤ 0.05. The development of infectious diseases is significantly associated with the geographical zone (p = 0.001). The population of Acatzingo de Hidalgo had a higher predicted probability (54.8%) of developing infections. Zone 3, which is exposed to hydrocarbons, had twice the probability of contracting infections (OR = 1.833, p = 0.093). Factors such as tobacco or alcohol consumption, gender, and age did not influence the development of infectious diseases. However, minors, businesspeople, and individuals with chronic degenerative diseases were more likely to contract infectious diseases. Therefore, it is crucial to implement control and regulation in managing pesticides and hydrocarbon spills to mitigate environmental contamination and the associated risks to human health.

Inequity In Rural Communities (Sanaguin-Molleturo) On the Proper Management of Pesticides and Its Consequences on Public Health

Inequity in rural areas regarding the proper management of pesticides, especially in communities where their main economic activity is agriculture, it is important to prioritize environmental training issues that contribute to Public Health in the least favored sectors, so in this in the document, the community of Sanagüin, which has approximately 100 inhabitants, is selected. The economic activity is based mainly on the cultivation and production of the land, generally this work is carried out manually, and very little mechanized and technical; Products such as sugar cane are grown mostly in this community. As an alternative activity and that has gained strength in recent years are grass crops for livestock, especially in the highlands where the inhabitants have dedicated themselves to fattening cattle. In the community of Sanagüin, the indiscriminate use of pesticides results in a direct impact on the health of the population, in addition to environmental and soil contamination, so this work is based on the collection of primary and secondary data that shows mismanagement of pesticides in different crops. To support and quantify the inequity of knowledge of reference to the indiscriminate use of chemical products, such as a local one carried out at the level of the different communities at a rural level in the province of Azuay, blood tests were carried out that justify a level of mild long-term intoxication. term by means of Acetylcholinesterase; since the results found show levels that are outside the normal range.

Overexpression of cytochrome P450 and esterase genes involved in permethrin resistance in larvae and adults of Culex quinquefasciatus.

Mosquitoes are important vectors of several arthropod-borne diseases, which remain a priority for epidemiological research. Mosquito vector control strategies have traditionally relied on chemical insecticides such as synthetic pyrethroids. However, the indiscriminate use of pesticides has resulted in the development of resistance in many mosquito species. In insects, resistance evolves primarily through the overexpression of one or more gene products from the cytochrome P450, carboxylesterase, and glutathione superfamilies. The current study examined the expression of cytochrome P450 CYP6M2, CYP6AA7, CYP6Z2, CYP9J34, α-Esterase, Esterase B1, and neuroactin genes in larvae and adults of a permethrin-resistant (PerRes) and susceptible (Sus) Culex quinquefasciatus strains. The results showed that the CYP6AA7 gene was overexpressed (10-fold) in larvae and adults with PerRes (p < 0.01) followed by CYPJ34 (9.0-fold) and CYP6Z2 (5.0-fold) compared to the Sus, whereas fewer changes in CYP6M gene expression were observed in PerRes adults (p < 0.05), and no expression was found in larvae. The esterase gene was overexpressed in PerRes larvae (9.0-fold) followed by adults (2.5-fold) compared to the susceptible strain. Based on data, the present study suggests that cytochrome P450, CYP6AA7, CYP6Z2, CYP9J34, α-Esterase, Esterase B1, and neuroactin genes were involved in permethrin resistance in larval and adult Cx. quinquefasciatus.

Inventory of pesticides and their potential risk of contamination in the semi-arid region of Ceará (Cariri–CE, Brazil)

The present study deals with pesticides, and their potential risk of contamination in the municipalities of the Cariús River micro-watershed (Cariri – CE). In general terms, it aimed to analyze the environmental fragility to the use of pesticides and to identify the active principles used, their respective environmental risks and their process of accumulation, transport and contamination of soil and water, in the area that covers the municipalities of Santana do Cariri, Nova Olinda, Farias Brito and Cariús, located in the south of the state of Ceará. To carry it out, the GUS Index (Groundwater Ubiquity Score) was applied in the active principles of pesticides sold and applied in the region, as in a model suggested by Neves, Gomes and Spadotto (1998), which takes into account the leaching and resulting surface runoff variables, and the natural characteristics (Pedology, hydraulic conductivity, altitude and slope) in the delimitation of areas with natural susceptibility to environmental impacts resulting from the application of pesticides. The results indicated that the entire region is susceptible to contamination caused by the indiscriminate use of pesticides, with great potential for contamination of groundwater and surface water. Due to their high leaching capacity, the following products should be abolished: Connect, Atrazine Nortox 500 SC, Calaris, Nicosulfuron Nortox 750 WG, DMA® 806 BR, Tucson, Quallis, U46, Artys, Aminol, Tordon and Disparo®. Glyphosate, Karate Zeon 50 CS, Klorpan, Pyrinex 480 EC, and Decis 25EC should be avoided or replaced with alternative techniques. It is recommended to diversify the production and promotion of organic agriculture through sustainable agricultural techniques/practices and with lower environmental impacts.

Management of Two-Spotted Spider Mite, Tetranychus Urticae Koch Infesting Gerbera with biopesticides and Predatory Mite

Among various flowers, gerbera holds a prominent place and a good market in India, but it can be attacked by the two-spotted spider mite, Tetranychus urticae Koch. Indiscriminate use of pesticides has led to the development of resistance in this spider mite species, affected human health and also caused environmental pollution. For safer and more cost-effective and environmentally friendly alternative approaches for the management of this pest is desirable. Different combinations of chemical pesticides, plant products, entomopathogenic fungi, and predatory mite, Neoseiulus longispinosus were tested for four years (2017-2020). The results showed that the ctreatment combination of propargite (0.05%) + propargite (0.05%) + propargite (0.05%) was found most effective against spider mite, T. urticae and were followed by the treatment combination of neem oil (0.5%) + neem oil (0.5%) + release of 20 gravid females of predatory mite, N. longispinosus.

Hematological Responses of Tilapia Sarotherodon mossambicus (Peters) Exposed to the Pesticide, Sevin

Since the blood takes part directly or indirectly in various physiological processes of the body, hematological parameters are considered important biomarker of alterations in metabolism or physiology. Indiscriminate use of pesticides has polluted different water bodies with adverse effects on the health of aquatic biota including fishes. Toxicological effects of agrochemicals including pesticides can be observed by monitoring hematological parameters. Present review deals with study of effects of pesticides on the important blood parameters such as erythrocyte count, hemoglobin content, packed cell volume, erythrocyte sedimentation rate, absolute values, leucocyte count, coagulation time and thrombocyte count of freshwater fish species. The review also aims to explain possible mechanism of pesticide induced alterations in the parameters and deleterious consequences on fish health. Tilapia (Sarotherodon mossambicus) were exposed to 5, 10, 20, 40, &amp; 60% concentration of the 96 hours, LC 50 value of Sevin for 7, 14 and 21 days. There was a significant decrease in the levels of hemoglobin and hematocrit of tilapia Sarotherodon mossambicus exposed to varied concentrations of Sevin indicating stress at higher concentrations.

The Use of Multiple Biomarkers to Assess the Health of Anuran Amphibians in the Brazilian Cerrado Savanna: An Ecotoxicological Approach.

Changes in the natural landscape and the indiscriminate use of pesticides can have a major impact on aquatic environments and have contributed to the worldwide decline of amphibian populations. In the present study, we sampled tadpoles of three anuran amphibians (Boana albopunctata, Physalaemus cuvieri, and Dendropsophus minutus) from ponds in six different agricultural landscapes of the Brazilian Cerrado savanna and evaluated whether and to what extent genotoxic and mutagenic damage was related to land use (the amount of forest and agricultural remnants, and related physicochemical factors) and the presence of pesticides in the water of the study ponds. We also evaluated the hepatotoxicity in P. cuvieri, which was the most abundant species at five of the six sampling points. Clomazone and atrazine were the most common pesticides found in the ponds. The B. albopunctata and P. cuvieri tadpoles presented similar patterns of DNA damage among the sampling points. The least DNA damage was found in the D. minutus tadpoles, although this species was present in only one of the study ponds. More binucleated and anucleated cells were observed in B. albopunctata, but there was no significant variation among species in terms of the number of micronuclei or other erythrocytic nuclear abnormalities. Land use and physicochemical factors did not explain the variation in the DNA damage observed in the three anurans. The hepatotoxicity analyses of P. cuvieri revealed the presence of a series of alterations, including the enlargement of the sinusoids, vacuolization of the hepatocytes, the infiltration of inflammatory cells, hepatic steatosis, and dilation of the blood vessels. The interaction between physicochemical factors and the biomarkers analyzed in the present study is complex. In particular, it will be important to better elucidate which factors are contributing, either directly or indirectly, to the decline of anuran amphibian populations, especially in threatened biomes, such as the Brazilian Cerrado. In this case, we would encourage further in situ studies that assess the ecotoxicology of the landscape, together with the systematic monitoring of aquatic environments, to guarantee the long-term integrity of amphibian populations, and those of other organisms that play an essential functional role in the ecosystem. Environ Toxicol Chem 2023;00:1-18. © 2023 SETAC.

Epilithic biofilms as a discriminating matrix for long-term and growing season pesticide contamination in the aquatic environment: Emphasis on glyphosate and metabolite AMPA

The indiscriminate use of pesticides represents high ecological risk in aquatic systems. Recently, the inclusion of epilithic biofilms as a reactive matrix has shown potential in diagnosing the health of water resources. The objective of this study was to use multiple matrices (water, suspended sediments, and biofilms) to discriminate contamination degrees in catchments with long and recent history of intensive pesticide use and to monitor growing season pesticides transfer to watercourses. Two catchments were monitored: one representative of “modern agriculture” in a subtropical environment, and another representative of recent agricultural expansion over the Pampa Biome in subtropical Brazil. Glyphosate and AMPA were accumulated in the biofilms and were detected at all sites and at all monitoring times, in concentrations ranging from 195 to 7673 μg kg−1 and from 225 to 4180 μg kg−1, respectively. Similarly, the fungicide tebuconazole has always been found in biofilms. The biofilms made it possible to discriminate the long-term history of pesticide use in the catchments and even to identify the influx pulses of pesticides immediately after their application to crops, which was not possible with active water sampling and even with suspended sediment monitoring. It is strongly recommended that, in regions with intensive cultivation of soybeans and other genetically modified crops, the presence of glyphosate and its metabolite AMPA be permanently monitored, a practice still very scarce in the literature.

Molecular mechanism of kidney damage caused by abamectin in carp: Oxidative stress, inflammation, mitochondrial damage, and apoptosis

Indiscriminate use of pesticides not only leads to environmental pollution problems, but also causes poisoning of non-target organisms. Abamectin (ABM), a widely used insecticide worldwide, is of wide concern due to its persistence in the environment and its high toxicity to fish. The kidney, as a key organ for detoxification, is more susceptible to the effects of ABM. Unfortunately, few studies investigated the mechanisms behind this connection. In this study, carp was used as an indicator organism for toxicological studies to investigate renal damage caused by ABM residues in carp. In this work, carp were exposed to ABM (0, 3.005, and 12.02 μg/L) for 4 d and the nephrotoxicity was assessed. Histopathological findings revealed that ABM exposure induced kidney damage in carp, as well as an increase Creatinine and BUN levels. Meanwhile, ABM as a reactive oxygen species (ROS) stimulator, boosted ROS bursts and lowered antioxidant enzyme activity while activating the body's antioxidant system, the Nrf2-Keap1 signaling pathway. The accumulation of ROS can also lead to the imbalance of the body's oxidation system, leading to oxidative stress. At the same time, NF-κB signaling pathway associated with inflammation was activated, which regulated expression levels of inflammatory cytokines (TNF-α, IL-6, IL-1β, and iNOS increased, while IL-10 and TGF-β1 decreased). In addition, ABM exposure caused structural damage to kidney mitochondria of carp, resulting in decreased mitochondrial membrane potential and ATP production capacity, and mediated apoptosis through endogenous pathways Bax/Bcl-2/Caspase-9/Caspase-3. In conclusion, ABM caused kidney damage in carp by inducing oxidative stress, inflammation, and apoptosis through mitochondrial pathway. These findings will be useful for future research into molecular mechanisms of ABM-induced nephrotoxicity in aquatic organisms.

Bioatividade de extratos aquosos de folhas de plantas na biologia de Spodoptera frugiperda (J.E.Smith, 1797) (Lepidoptera: Noctuidae)

The armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is a key pest of corn, as it causes defoliation to plants throughout the year, causing significant economic losses. The most common method for its control is chemical, but the indiscriminate use of pesticides, in addition to causing adverse effects to humans and the environment due to the toxicity of the products, favors the development of resistant individuals, thus causing the insect in question is difficult to control. Plants popularly known as myrtle (Murraya paniculata), guaco (Mikania glomerata), são Caetano melon (Momordica charantia) and lemon balm (Melissa officinalis) have demonstrated pharmacological potential, presenting repellent or toxic effects to insects. Thus, the objective of this work was to analyze in the laboratory the insecticidal activity of M. paniculata, M. glomerata, M.charantia and M. officinalis on the biological parameters of S. frugiperda, through the incorporation of extracts in artificial diets. Based on the results obtained, a significant insecticidal activity of the myrtle leaf extract (Murraya paniculata) was observed on S. frugiperda, as it caused a significant reduction in larval and pupal viability.