For a scientific and reliable design of asphalt mixture subjected to fatigue damage, a comprehensive understanding and realistic prediction of asphalt mixture fatigue life under complex loading conditions with variable loading rates or temperature were a major concern. In this study, direct tensile test, uniaxial compression test, indirect tensile test, and four-point bending test were used to study the fatigue behavior of asphalt mixtures with a particular focus on the action of temperature and frequency. Strength and fatigue were investigated separately under varied loading rates at several temperatures. A new idea for determining the stress ratio based on the strength failure envelope line considering temperature and loading rate was proposed. The connection between strength and fatigue was established by the stress ratio. The test conditions for strength and fatigue tests were set to be the same leading to the fatigue data distributed regularly. Experimental data were analyzed and normalized by studying the evolution of the number of cycles with the stress ratio. A new analytical approach was introduced to simulate the fatigue performance. The fatigue performance under multi-field coupling conditions was uniformly characterized. The dispersion of fatigue equation caused by the test method and conditions is eliminated. Engineering application showed that the design deviation of the pavement fatigue resistance was reduced to less than 6%.