Indirect Sampling Method Research Articles

Sea slugs (Gastropoda: Heterobranchia) associated with Autonomous Reef Monitoring Structures (ARMS) in southern Gulf of Mexico and Mexican Caribbean Sea

Abstract Cryptic species in coral reefs, such as sea slugs, represent an important portion of their biodiversity, which is usually underestimated. Autonomous Reef Monitoring Structures (ARMS) have been implemented to estimate cryptic diversity in coral reefs. Therefore, this research aimed to contribute to the southern Gulf of Mexico (GM) and the Caribbean Sea (CAR) coral reefs’ sea slugs’ diversity and distribution using ARMS as a collection method. Fifty-eight ARMS were placed at three coral reefs in the GM and CAR, recovered after 1–2 years and then, disassembled at the laboratory. Plates were individually placed in trays with seawater, where we searched for sea slugs. A total of 242 organisms were found belonging to 31 species; 20 of them were identified to the species level, while 11 were determined up to genus or family. More than half of the species (19) were found in Bajo de 10 (GM), while 15 species were found in the CAR localities. Unlike previous studies, we assessed sea slugs’ diversity exclusively by an indirect sampling method. In this work, we found 9.4% of the sea slug diversity recorded in the Caribbean, and we report four determined species for the first time in the country. New records of species, and even one family for the GM stress the gap of information that we still need to fulfil in the area. We recognize ARMS as a useful tool to find juvenile, cryptic and rare species of sea slugs, as well as to standardize their quantification and record their diversity.

Xây dựng giá trị tham chiếu các chỉ số xét nghiệm Sinh hóa tại Trung tâm Xét nghiệm, Trường Đại học Y tế công cộng

Objective: Establish biochemistry reference intervals suitable for the Laboratory center of Hanoi University of Public Health. Methods: The study was carried out based on guidance of CLSI EP28-A3 using indirect sampling method. 8345 normal sample from January 2018 to April 2021 in Laboratory center of Hanoi University of Public Health is used. Results: The results showed that the biochemistry reference intervals was established as below: GLU (3.94-6.38 mmol/L), men (3.94-6.48 mmol/L), women (3.95-6.26mmol/L); CHOL (3.46- 6.06 mmol/L), male (3.55-6.10 mmol/L), female (3.44-6.02 mmol/L); TRI (0.46-2.20 mmol/L), male (0.56-2.24 mmol/L), female (0.44-2.11 mmol/L); URE (2.62-7.10 mmol/L), men (2.93-7.32mmol/L), women (2.48-6.72mmol/L); CRE (53.62-107.09 µmol/L), men (68.58-111.14 µmol/L ), women (50.35-86.54 mol/L); AST (13.46-32.27 U/L, men (14.05- 32.83U/L), female (13.03-30.99 U/L); ALT (7.21-34.14 U/L), men (7,91-35,06 U/L), female (13,03-30,99 U/L). Conclusions: The reference intervals determined in our research have the significant differences to the reference intervals currently used in our laboratory. Key words: Reference interval, Laboratory Center, Hanoi University of Public Health

Accelerated Sampling Research Under Task Optimized Strategy for Multistate Systems

The complex multi-state systems joint operation simulation and two task strategies are explored. For Monte Carlo sampling, based on the indirect sampling method, the principle of accelerated sampling methods, including forced transition FT and fault biasing FB, is studied. It is found that the direct adoption of the current FT and FB will result in systematic bias for the calculation of task success metrics. For the bias, state-transition chain truncation is studied, and its definition and truncation rules are proposed. In case study, after the simulation of various parameter combinations, it is found that when the sailing time is relatively smaller than the equipment reliability, which means a small probability event for the sampling of equipment faults, the ASM based on indirect sampling is applicable. Otherwise, the conventional sampling method should be used. The proposed state-transition chain truncation rule is also verified in case study.

Reference intervals of automated reticulocyte count and immature reticulocyte fraction in a pediatric population.

Reticulocytes are erythroid precursors that develop into mature erythrocytes, and they are an important tool to assess erythropoietic activity, as their count indicates the balance between the cells released from the bone marrow, their stage of maturity, and their rate of development into mature erythrocytes. Considering the described biological variability of the absolute reticulocyte count (ARC) and the immature reticulocyte fraction (IRF) and the limited information available on these hematological parameters in children, this study determined the reference intervals (RIs) of these parameters in a healthy pediatric population. A retrospective, observational, and analytical study was designed to establish RIs for the ARC and the IRF according to age and sex. An indirect sampling method was applied to a mixed database of complete blood counts from children aged 2months to 18years, using the truncated maximum likelihood indirect method for reference interval estimation. Percentiles were calculated to obtain bimodal RIs. From a total of 190,812samples, 6,814 were selected. Gender stratification was not necessary for the ARC and the IRF but they required partitioning into six and two age groups, respectively. This study determined, by an indirect sampling method, RIs for the ARC and the IRF in a pediatric population according to age and sex.

Hematological and Biochemical Reference Intervals for 5 Adult Hunting Dog Breeds Using a Blood Donor Database.

Simple SummaryHematological and biochemical profiles are essential in the diagnosis and monitoring of disease in veterinary medicine, requiring optimal Reference Intervals (RIs) for accurate interpretation. The aim of this study is to determine hematobiochemical RIs for 5 hunting dog breeds from a blood donor database and to compare them with laboratory established and published RIs to identify possible breed and attitude-related differences. A total of 445 healthy adult hunting dogs (156 Ariégeois, A; 52 Bleu de Gascogne, B; 64 Bracco italiano, C; 123 Segugio italiano, D; 50 Briquet Griffon Vandeen, E) were included in the study. Significant differences in 12 hematologic and serum biochemical analytes, for which a breed-specific variation appears to be the most plausible explanation, were detected, and new RIs for these parameters are provided.Numerous studies have shown the importance of breed-related differences between hematological and biochemical results in veterinary medicine. The aim of this study is to determine hematologic and biochemical Reference Intervals (RIs) for 5 hunting dog breeds from a blood donor database, adopting an indirect sampling method, and to compare them with laboratory established and published RIs to identify possible breed and attitude-related differences. The study analyzed the blood parameters of 445 adults (222 females and 223 male, with age ranging from 2 to 8 years, mean age 5.3 years), client-owned, clinically healthy blood donor dogs of 5 breeds: 156 Ariégeois, 52 Bleu de Gascogne, 64 Bracco italiano, 123 Segugio italiano, and 50 Briquet Griffon Vandeen. Statistical analysis was performed as recommended by the American Society of Veterinary Clinical Pathology (ASVCP) guidelines. RIs for red blood cells (RBC), hematocrit (HCT), hemoglobin (HB), main corpuscular volume (MCV), main corpuscular hemoglobin (MCH), main corpuscular hemoglobin concentration (MCHC), red distribution widht (RDW), white blood cells (WBC), and differential leukocytes count, PLT, Albumin, Total Protein, Urea, Creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) for each of the 5 breeds were performed, and significant differences with the established RIs were detected. We found significant differences in 12 hematologic and serum biochemical analytes for which a breed-specific variation appears to be the most plausible explanation. New RIs for HCT, MCH, MCHC, RDW, PLT, Monocytes, Eosinophils, Albumin, Urea, Creatinine, AST, and ALT are provided for at least 1 breed. Breed-specific RIs for adult hunting dogs will help avoid misinterpretation of laboratory results in these breeds.

Characterizing Solvent Density Fluctuations in Dynamical Observation Volumes.

Hydrophobic effects drive diverse aqueous assemblies, such as micelle formation or protein folding, wherein the solvent plays an important role. Consequently, characterizing the free energetics of solvent density fluctuations can lead to important insights into these processes. Although techniques such as the indirect umbrella sampling (INDUS) method can be used to characterize solvent fluctuations in static observation volumes of various sizes and shapes, characterizing how the solvent mediates inherently dynamic processes, such as self-assembly or conformational change, remains a challenge. In this work, we generalize the INDUS method to facilitate the enhanced sampling of solvent fluctuations in dynamical observation volumes, whose positions and shapes can evolve. We illustrate the usefulness of this generalization by characterizing water density fluctuations in dynamical volumes pertaining to the hydration of flexible solutes, the assembly of small hydrophobes, and conformational transitions in a model peptide. We also use the method to probe the dynamics of hard spheres.

Reference intervals for canine hematologic analytes using Siemens Advia 120

Hematologic investigation is essential for the evaluation of health status of companion animals. Appropriate and accurate reference intervals (RIs) are required for the interpretation of laboratory results. Thus, the primary aim of the present study was to establish canine complete blood count (CBC) RIs using Advia 120, a widely used in veterinary medicine automated hematology analyzer. Additional objectives were to evaluate sex as a partitioning factor of RIs and to investigate the effect that breed size has on CBC RIs. Reference individuals were selected by indirect sampling method from the medical records of a veterinary teaching hospital. The reference population comprised 284 adult dogs of both sexes and various breeds. The reference individuals were allocated into 3 groups based on breed size (small-sized, medium-sized and large-sized breeds). Complete blood count results from the dogs that met the inclusion criteria were used for the nonparametric calculation of RIs. Statistical and nonstatistical criteria were employed in order to decide whether sex-specific RIs are needed. Depending on the data distributions, mean or median comparisons were used to determine the effect of breed size and lifestyle on CBC results. Nine outliers were detected based on CBC results. The estimated RIs were generally comparable to those previously reported in the literature. Sex-dependent partitioning of RIs was indicated by the statistical criteria for a few analytes. From a clinicopathologic point of view though, sex-dependent partitioning of RIs is questioned and seems not to be required. Breed size appears to have an effect on CBC RIs. The RIs determined in the present study can be used as a guide for the interpretation of CBC results in dogs and can potentially be adopted by veterinary laboratories using Advia 120. Finally, based on the results of this study, breed size should probably be considered when interpreting CBC results.

Environmental stressors may cause equine herpesvirus reactivation in captive Grévy's zebras (Equus grevyi).

Equine Herpesviruses (EHV) are common and often latent pathogens of equids which can cause fatalities when transmitted to non-equids. Stress and elevated glucocorticoids have been associated with EHV reactivation in domestic horses, but little is known about the correlation between stress and viral reactivation in wild equids. We investigated the effect of an environmental stressor (social group restructuring following a translocation event) on EHV reactivation in captive Grévy’s zebras (Equus grevyi). A mare was translocated by road transport from Zoo Mulhouse, France, to join a resident group of three mares in Tierpark Berlin, Germany. We used an indirect sampling method to assess the frequency of EHV shedding for 14 days immediately after the translocation event (termed the ‘experimental period’). The results were compared with those from two control periods, one preceding and one subsequent to the experimental period. In addition, we measured fecal glucocorticoid metabolite (fGCM) concentrations daily in all individuals from 6 days before, to 14 days after translocation. We found significantly higher EHV shedding frequencies during the experimental period, compared to each of the two control periods. All animals showed significantly elevated fGCM concentrations, compared to fGCM levels before translocation. Finally, we found that an increase in fGCM concentration was significantly associated with an increased likelihood of EHV shedding. Although the small number of animals in the study limits the conclusions that can be drawn from the study, taken together, our results support the hypothesis that environmental stressors induce viral reactivation in wild equids. Our results suggest that potentials stressors such as group restructuring and translocation should be considered in the management of zoological collections to reduce the risk of fatal EHV infections in novel hosts. Moreover, environmental stressors may play an important role in EHV reactivation and spread in wild equid populations.

Derivation of pediatric within-individual biological variation by indirect sampling method: an LMS approach.

Pediatric within-individual biological variation (CVi) is a challenge to derive by direct sampling due to clinical, logistical, and ethical barriers. Laboratory results of 22 basic biochemistry tests performed on 9,356 children who visited primary care physicians more than once over a year were obtained from a large laboratory network in Australia. The CVi were calculated as (CVT (2) - CVa (2))(0.5), where CVT was the coefficient of variation between repeat measurements and CVa was the analytical imprecision. Smoothed 50th centile (median) CVi charts were derived using the LMS ChartMaker Light software (Medical Research Council, Cambridge, England) with L, M, and S parameters fixed at 3.0, 3.0, and 3.0 equivalent degrees of freedom, respectively. In general, the median CVi trends for this pediatric cohort remained relatively stable with increasing age. Only aspartate aminotransferase, globulin, phosphate, urea, and creatinine had differences between the highest and lowest median CVi of more than 30%. The differences between the child and adult CVi were relatively small. Nearly all the analytes had child to adult CVi ratios of 1.0 ± 0.5. The median CVi derived from patients with only two repeat biochemistry measurements may be considered reasonable estimates of CVi among children seeking treatment at primary care settings. The LMS approach allowed visualization of the continuous trends of CVi with age and extended the pediatric CVi estimation to younger than 4 years.

Use of an Indirect Sampling Method to Produce Reference Intervals for Hematologic and Biochemical Analyses in Psittaciform Species

As with other animal species, comprehensive reference intervals (RI) for psittaciform species are rare and plagued by common issues, including sparse information regarding methods used to analyze specimens, low sample sizes, and improper statistical analyses. The purpose of this study was to examine the use of an indirect sampling method of RI generation from several years of data collected from specimens of multiple psittaciform species submitted to a veterinary diagnostic laboratory. These data were unselected for health status. A previously published method for indirect RI generation was applied to data collected for routine hematologic and biochemical analyses. Seven species groups were examined, and sample size ranged from 346 to 2358. Results showed that RI varied by species and appeared to represent a broader range than expected compared with other RI and traditional clinical expectations for core health assessments, such as total white blood cell count and white blood cell differential results. Some biochemical results reflected more narrow ranges, and a few were consistent with other published ranges. The intervals were likely influenced by changes related to stress and underlying disease. The results of the current study reflect the imprecision of this method related to data obtained from the population served by this laboratory. Overall, this method is not suitable for the production of comprehensive RI, although it may provide rough estimates for some limited analyses until traditional RI can be generated.

Establishment of canine hematology reference intervals for theSysmexXT‐2000iV hematology analyzer using a blood donor database

The Sysmex XT-2000iV is a hematology analyzer that combines impedance and optical techniques and has been previously validated for dogs. Specific reference intervals (RIs) are useful when interpreting results. The aim of this study was to determine hematologic RIs for the Sysmex XT-2000iV using a large reference population of client-owned clinically healthy blood donor dogs, adopting an indirect sampling method. Dogs were screened for breed, size, health, travel history, and previous blood transfusions, and the quality of blood specimens was also reviewed. Results from specimens that met inclusion criteria were used to determine RIs using a nonparametric method. Specimens from Akitas and sighthounds were excluded from the study. Of 992 specimens that had been collected from blood donors and analyzed, 297 were initially included in the RI study. An additional 38 specimens were excluded as outliers, and hematologic RIs for the Sysmex XT-2000iV were based on analysis of specimens from 259 clinically healthy dogs. Measurands evaluated had variable distributions, and intervals obtained were generally comparable to previously reported RIs. Differences observed included higher lower and upper reference limits (LRL and URL, respectively) for MCV and lower URL for WBC count. Reticulocyte count and the LRL of the absolute lymphocyte count were also higher than previously reported, and the RI for platelet count was narrower and lower. Canine RIs for the Sysmex hematology analyzer were established using an indirect sampling method with reference individuals selected from a large database of client-owned clinically healthy blood donor dogs. For specimens included in this study, time from collection to analysis was similar to what veterinary commercial laboratories experience.

Quantifying density fluctuations in volumes of all shapes and sizes using indirect umbrella sampling.

Water density fluctuations are an important statistical mechanical observable that is related to many-body correlations, as well as hydrophobic hydration and interactions. Local water density fluctuations at a solid-water surface have also been proposed as a measure of it's hydrophobicity. These fluctuations can be quantified by calculating the probability, P(v)(N), of observing N waters in a probe volume of interest v. When v is large, calculating P(v)(N) using molecular dynamics simulations is challenging, as the probability of observing very few waters is exponentially small, and the standard procedure for overcoming this problem (umbrella sampling in N) leads to undesirable impulsive forces. Patel et al. [J. Phys. Chem. B, 114, 1632 (2010)] have recently developed an indirect umbrella sampling (INDUS) method, that samples a coarse-grained particle number to obtain P(v)(N) in cuboidal volumes. Here, we present and demonstrate an extension of that approach to volumes of other basic shapes, like spheres and cylinders, as well as to collections of such volumes. We further describe the implementation of INDUS in the NPT ensemble and calculate P(v)(N) distributions over a broad range of pressures. Our method may be of particular interest in characterizing the hydrophobicity of interfaces of proteins, nanotubes and related systems.

Indirect Evidences of Wildlife Activities in Shoals of Western Ghats, a Biodiversity Hotspots

Indirect Evidences of Wildlife Activities in Shoals of Western Ghats, a Biodiversity HotspotsThe presence of wildlife fauna and its activities were ascertained with the density of the scat, dung and other markings or droppings of the wildlife abode therein. Attempt was made to find out spatial differences in the activities of the wildlife populations and to comment on the abundance of different preys and predators within shola forests of Western Ghat hill forests, a Biodiversity hotspot in India. An indirect sampling method, Transect Count Method, was employed to count dung/pellet group/scat and other markings in that area. Pachyderms were found to be mostly dominant in Varagaliar and Punnumala shola patches while scats of all the three important predators, viz., tiger (Panthera tigris tigris), Indian wild dog (Cuon alpinus) and leopard (Panthera pardus) were encountered only in Varagaliar shola. Greater abundance was recorded from Indira Gandhi Wildlife Sanctuary than Silent Valley National Park may be because of the restriction of animal movements in the former due to topographical barriers and its existence as isolated shola patches that led to a greater concentration of wild fauna in a relatively segregated forest cover.

Construction and Field Application of an Indirect Sampling Method (Time-Location Sampling): An Example of Surveys Carried Out on Homeless Persons and Drug Users in France

Since the mid-1990s, Time-Location Sampling methods have been widely used in France in quantitative research about the homeless (Marpsat and Firdion, 2000; Brousse, 2002) and drug users (Emmanuelli et al. 2003; Jauffret-Roustide et al. 2006). Adapted from methods of indirect sampling initially developed in the United States (Burt and Cohen, 1989; Dennis and Iachan, 1993), they enable the design of representative samples of hard-to-reach populations for whom there are no sample frames, by basing it on support facilities for the populations of interest: day centres, soup kitchens, accommodation services, health care, mobile teams etc. However, in transition from the theoretical protocol to its implementation, various difficulties emerge. In particular, the necessary involvement of these support services; the constraints associated with the administering of questionnaires in sometimes very particular conditions (at night, in public places or while following mobile teams) and the singular organisation of each of the services that has to be involved means that challenges occur as regards coverage of the field being investigated and the representativeness of the sample. These challenges mean that the method has to be appropriately and pragmatically adapted to the various configurations that are met with. In this perspective, we will show how the integration of qualitative methods, such as the observation of the services and the realisation of in-depth interviews with the persons in charge of them, have contributed, along the various data collections and with the accumulation of experiences, to improve the methodology of and the scope covered by quantitative surveys.

Comparison of Direct and Indirect Sampling Methods for Tarnished Plant Bug (Hemiptera: Miridae) in Flowering Cotton

A complex of hemipterans, especially the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), has become a major target of insecticides in flowering cotton, Gossypium hirsutum L., in the mid-southern United States. Sampling protocols for this complex during this period of cotton development are poorly established, resulting in uncertainty about when infestations warrant treatment. Nine direct and indirect sampling methods were evaluated for bias, precision, and efficiency in cotton throughout the Mid-South during 2005 and 2006. The tarnished plant bug represented 94% of the bug complex in both years. Sweep-net and black drop-cloth methods were more efficient than other direct sampling methods, but they were biased toward adults and nymphs, respectively. Sampling dirty blooms was the most efficient indirect sampling method. The sweep-net, whole-plant, and dirty-bloom methods were more accurate than the other sampling methods evaluated based on correlations with other sampling methods. Variability attributed to the person collecting the sample was significant for all sampling methods, but least significant for the dirty-square method. Further research is needed to establish thresholds based on sweep-net, drop-cloth, dirty-square, and dirty-bloom sampling methods as these methods provide the best combinations of accuracy and efficiency for sampling tarnished plant bugs in cotton.

Comparison of Direct and Indirect Sampling Methods for Tarnished Plant Bug (Hemiptera: Miridae) in Flowering Cotton

A complex of hemipterans, especially the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), has become a major target of insecticides in flowering cotton, Gossypium hirsutum L., in the mid-southern United States. Sampling protocols for this complex during this period of cotton development are poorly established, resulting in uncertainty about when infestations warrant treatment. Nine direct and indirect sampling methods were evaluated for bias, precision, and efficiency in cotton throughout the Mid-South during 2005 and 2006. The tarnished plant bug represented 94% of the bug complex in both years. Sweep-net and black drop-cloth methods were more efficient than other direct sampling methods, but they were biased toward adults and nymphs, respectively. Sampling dirty blooms was the most efficient indirect sampling method. The sweep-net, whole-plant, and dirty-bloom methods were more accurate than the other sampling methods evaluated based on correlations with other sampling methods. Variability attributed to the person collecting the sample was significant for all sampling methods, but least significant for the dirty-square method. Further research is needed to establish thresholds based on sweep-net, drop-cloth, dirty-square, and dirty-bloom sampling methods as these methods provide the best combinations of accuracy and efficiency for sampling tarnished plant bugs in cotton.

Sevoflurane low‐flow anaesthesia: best strategy to reduce Compound A concentration

To define the best strategy to reduce Compound A production in Sevoflurane low-flow anaesthesia by experiments in vitro and in vivo of different absorbers and different anaesthesia machines. In vitro Compound A has been measured at 45 degrees C in vitro following Sevoflurane interactions with potassium hydroxide, sodium hydroxide, soda lime, Dragersorb 800 Plus and Amsorb, a new absorber that does not contain sodium or potassium hydroxide. In vivo Compound A concentration in the anaesthesia circuit (inspiratory branch) has been measured using an indirect sampling method through absorber vials (SKC) with active coal granules, during low flows (500 ml/min) general anaesthesia using soda lime, Dragersorb 800 Plus or Amsorb as absorber. Compound A was also measured during low flows (500 ml/min) general anaesthesia using as carbon dioxide absorber soda lime with different anaesthesia machines. In vitro at 45 degrees C Compound A concentration with soda lime and Dragersorb 800 Plus was about 10 times higher than with Amsorb. In vivo the Compound A concentrations in the inspiratory branch of the circuit were lower in the group with Amsorb. The Compound A production is minimal with Amsorb as carbon dioxide absorber.

Further Studies of Damage to Safflower Plants by Thrips and Lygus Bugs

Trials with western flower thrips, Frankliniella occidentalis (Pergande), confined on growing safflower buds for 34 days, demonstrated that the buds are able to tolerate fairly large numbers of nymphal thrips before the seed yield is reduced significantly. Adult thrips were introduced into small cages covering selected buds (¼ inch in diameter or less) on plants growing in the field. An indirect sampling method was used to estimate the subsequent buildup of the population of nymphs. The tests were set up with initial thrips densities adjusted to average 5, 10, 20, and 40 adults per head. The density of nymphs averaged as high as 75 per bud when 10 adults per bud were introduced into the cages, and there was no consequent drop in seed production. Approximately twice as many nymphs per bud developed when initial introductions averaged 20 adults per bud. Infestations started with 20 or 40 adults per bud decreased significantly the number of good seed heads produced, the number of seeds per head, and the total yield. Analogous trials with lygus bugs, Lygus hesperus Knight, indicated that the threshold of economic damage to the safflower crop was exceeded when the ratio of bugs to buds exceeded 1:8. Significant decreases in yield criteria were obtained when the bug to bud ratios were adjusted to 1:4 or higher. A new stripe, or thin-hulled, variety of safflower appeared to be more susceptible to injury by Iygus bugs than the variety U. S. 10. Seed yield of the variety U. S. 10 was not materially decreased when the plants were completely disbudded 13 days before the start of the blooming period. This operation reduced the number of seed heads which finally matured, but these bore larger and more numerous seeds than the seed heads of check plants. Complete disbudding or defoliation done at the onset of the blooming period caused very significant reductions in yield values. Attempts to improve seed yield with pesticides applied to control thrips and lygus bugs were unsuccessful, because the insect infestations and the plant damage incurred were not great enough to affect the yield.