Indigestible Neutral Detergent Fiber Research Articles

Validating intrinsic markers and optimizing spot sampling frequency to estimate fecal outputs

Indirect methods of spot sampling with intrinsic markers to estimate fecal output and nutrient digestibility often have been used in dairy nutrition research as alternatives to total collection of feces (TC) because of labor and expense. However, fecal output and nutrient digestibility estimated from the indirect method must be accurate regardless of altering dietary conditions. This experiment was designed to validate the accuracy of using indigestible neutral detergent fiber (iNDF) or acid-insoluble ash (AIA) as intrinsic markers to estimate fecal outputs and nutrient digestibility compared with TC and to determine the optimal number of spot sampling events to accurately determine fecal output and then nutrient excretion. The experiment used 12 multiparous lactating Holstein cows in a randomized complete block design. Cows were blocked by days in milk and milk yield and randomly assigned to 1 of 2 diets: a diet containing about 49% corn silage on a dry matter basis and a diet containing about 48% alfalfa silage with high by-product (soyhulls) and supplemental K. During the final 3 d of 21-d periods, TC was performed, and 12 spot samples were collected for the same 3 d to represent every 2 h in a 24-h cycle. Fecal outputs and nutrient digestibility of dry matter, organic matter, or nitrogen estimated with iNDF or AIA as an intrinsic marker were compared with TC. Overall, fecal outputs and digestibility estimated with iNDF were similar to that estimated with TC, whereas AIA overestimated fecal output by 44 to 61% and underestimated nutrient digestibilities by 16 to 32%. However, potential differences in statistical inference of dietary effects between iNDF and TC were found. Data from individual spot samples were aggregated to represent spot sampling frequencies of 12 (SP12), 6 (SP6), 4 (SP4), or 2 (SP2) evenly spaced events starting at feeding time. Compared with TC, SP12 produced similar fecal content of iNDF, organic matter, and nitrogen, but fecal AIA content was greater. Furthermore, compared with SP12, SP6 produced similar fecal content of all nutrients, whereas marker and nutrient concentrations in SP4 and SP2 were different. In this experiment, iNDF was a better fecal marker than AIA, and a spot sampling frequency of at least 6 events was necessary. However, interpretation of dietary effects could be confounded when iNDF was used to estimate fecal outputs.

Effect of variety and stage of maturity at harvest on nutrient and neutral detergent fiber digestibility of forage barley grown in western Canada

This study evaluated the effect of variety (V; CDC Cowboy, CDC Copeland, and Xena) and stage of harvest maturity (M; milk, early-, mid-, and hard-dough) on nutrient and neutral detergent fiber digestibility (NDFD) characteristics of barley forage using a randomized complete block design with 3 × 4 factorial treatment arrangement. Barley varieties had similar crude protein, but CDC Cowboy had greater (P < 0.01) acid detergent fiber (ADF), neutral detergent fiber (NDF), and lignin, and lower (P < 0.01) total digestible nutrient (TDN) content relative to Xena. Starch content of CDC Cowboy was lower (P < 0.01) than Xena at all stages of maturity with CDC Copeland intermediate at early- and mid-dough stages. Crude protein, ADF, NDF, and lignin content decreased (P < 0.01) while starch and TDN content increased (P < 0.01) with advancing maturity. Xena had greater (P < 0.01) NDFD, as determined by 6 h in vitro incubation using DaisyII system (NDFD6h), at milk, mid- and hard-dough stages of maturity than CDC Cowboy with CDC Copeland intermediate at mid-dough. However, CDC Cowboy had greater (P < 0.01) NDFD, as determined by 30 h in vitro incubation using DaisyII system (NDFD30h), at early-dough stage than Xena and greater NDFD30h at hard-dough stage than CDC Copeland. Xena had the lowest (P < 0.01) indigestible NDF based on 288 h ruminal in situ incubation (INDF288h), relative to CDC Copeland. These results indicate that to optimize NDFD30h, variety should be considered when deciding the timing of harvest.

Effects of maturity at harvest on the nutritive value and ruminal digestion of Eragrostis tef (cv. Moxie) when fed to beef cattle.

Teff (Eragrostis tef cv. Moxie), a warm-season annual grass, could be an excellent forage for beef cattle. However, there is limited information on its nutritive value to cattle when harvested at different stages of maturity. Thus, the objective of this research was to determine the effect of feeding teff hay harvested at the boot (BT), early-heading (EH), or late-heading (LH) stages of maturity on nutrient intake, ruminal fermentation characteristics, omasal nutrient flow, and N utilization in beef cattle. Six ruminally cannulated beef heifers (mean initial BW ± SD, 476 ± 32.6) were used in a replicated 3 × 3 Latin square design with 28-d periods (18 d for adaptation and 10 d for measurements). Dry matter intake was measured daily. Indwelling pH loggers were used to measure ruminal pH from days 21 to 28. Ruminal fluid and omasal digesta were collected from days 26 to 28 to determine fermentation characteristics and omasal nutrient flow. Fecal and urine samples to quantify N excretion were also collected (days 26 to 28). Blood samples for plasma urea-N (PUN) determination were collected 3 h post-feeding on day 28. There were no changes (P > 0.28) in the ADF or NDF content of teff with advancing maturity, but indigestible NDF increased (P < 0.01) with increasing maturity. Maturity had no effect (P ≥ 0.14) on DMI, and ruminal total short-chain fatty acid (SCFA) concentration, pH, digestibility, and outflow of DM, OM, NDF, ADF, and CP. However, the CP content of BT hay was greater (P < 0.01) than for EH and LH hay (18.1, 14.1, and 11.5%, respectively, DM basis), and this resulted in the higher CP intake (P < 0.01) for heifers fed the BT than the EH and LH hay. Consequently, ruminal ammonia-N (NH3-N) concentration was greater (P < 0.01) for heifers fed BT than EH and LH hay, thereby possibly explaining the tendency for a decrease (P = 0.08) in PUN concentration, and a decrease (P < 0.01) in the excretion of total N, urine N, and urea-N (UUN) with advancing maturity. However, fecal N excretion (g/d) did not differ (P = 0.76). In conclusion, despite a decrease in CP intake and ruminal NH3-N concentration, feeding beef heifers EH and LH compared to BT teff hay did not compromise ruminal digestion and outflow of DM, OM, NDF, ADF, and CP, and microbial protein synthesis. Advancing maturity in teff hay also resulted in a decrease in the excretion of total N and urine N and UUN when fed to cattle.

Evaluation of internal and external markers to estimate faecal output and feed intake in sheep fed fresh forage

The objectives of the present study were to estimate dry-matter intake and output of faeces using two external and four internal markers in sheep fed Brachiaria brizantha cv. Marandu on an ad libitum basis over 3- and 5-day periods. Six sheep fitted with ruminal cannulas were randomly assigned in a crossover design experiment to receive either of two treatments of external markers, namely titanium dioxide (TiO2) and chromic oxide (Cr2O3). Faecal output was obtained by total faecal collection and faecal grab sampling. Faeces were collected for 3- or 5-day periods, and, for each collection period, two sampling methods were compared; grab samples were collected directly in the rectum once daily, and a second sample was taken at the same time directly in faecal collection bags after having determined the daily total output of faeces. Faecal concentrations of the internal markers, indigestible dry matter, indigestible neutral detergent fibre, indigestible acid detergent fibre and indigestible acid detergent lignin (iADL), were determined. Faecal output was not accurately predicted with indigestible dry matter, indigestible neutral detergent fibre, indigestible acid detergent fibre and iADL. Dry-matter intake was predicted with iADL and TiO2 when faeces were collected for 5 days as grab samples once daily, or as total collection and with Cr2O3 when faecal grab samples were collected for 5 days. The results using external markers indicated that TiO2 is not a marker equivalent to Cr2O3 for estimating intake and faecal output. TiO2 was the only external marker to accurately estimate faecal output, independent of the method (total or grab) and time period (3 or 5 days) used; this suggests that TiO2 is the best marker tested for predicting the faecal output of sheep that are fed a diet of fresh Brachiaria brizantha (cv. Marandu) grass ad libitum.

Effect of concentrate feeding level on methane emissions, production performance and rumen fermentation of Jersey cows grazing ryegrass pasture during spring

Dietary supplementation has been well documented as an effective enteric methane (CH4) mitigation strategy. However, limited studies have demonstrated the effect of concentrate level on enteric CH4 emissions from grazing dairy cows, and to our knowledge none of these studies included a pasture-only diet or reported on rumen fermentation measures. Sixty multiparous (4.0 ± 1.51 SD) Jersey cows, of which six were rumen-cannulated, were used in a randomised complete block design, and the cannulated cows were used in a separate replicated 3 × 3 Latin square design, to investigate the effect of concentrate supplementation (0, 4, and 8 kg/cow per day; as fed) on enteric CH4 emissions, milk production, dry matter intake (DMI), and rumen fermentation of dairy cows grazing perennial ryegrass pasture during spring, following a 14-d adaptation period. The sulphur hexafluoride tracer gas technique was used to measure enteric CH4 emissions from 10 cows of each treatment group over a single 9-d measurement period. Parallel with the CH4 measurement period, pasture DMI was determined using TiO2 and indigestible neutral detergent fibre as external and internal markers, respectively, while milk yield, milk composition, cow condition, and pasture pre- and post-grazing measurements were also recorded. Total DMI (13.4 to 18.0 kg/d), milk yield (12.9 to 19.2 kg/d), energy corrected milk (14.6 to 20.7 kg/d), milk lactose content (46.2 to 48.1 g/kg) and gross energy intake (239 to 316 MJ/d) increased, while milk fat content (50.0 to 44.2 g/kg) decreased with increasing concentrate feeding level. Volatile fatty acid concentrations and ruminal pH were mostly unaffected by treatment, while dry matter disappearance decreased and NH3-N concentration increased with increasing concentrate feeding level. Methane production (258 to 302 g/d) and CH4 yield (20.6 to 16.9 g/kg of DMI) were similar for all cows, while pasture DMI (13.4 to 10.8 kg/d) and CH4 intensity (20.4 to 15.9 g of CH4/kg of milk yield) decreased linearly with increasing concentrate feeding level. Results indicate that concentrate supplementation on high quality pasture-only diets have the potential to effectively reduce CH4 emissions per unit of milk yield from grazing cows during spring.

Evaluating internal and external markers versus fecal sampling procedure interactions when estimating intake in dairy cows consuming a corn silage-based diet

Feed intake assessment is a valuable tool for herd management decisions. The use of markers, either internal or external, is currently the most used technique for estimating feed intake in production animals. The experiment used 10 multiparous Holstein cows fed a corn silage-based diet, with 55:45 forage-to-concentrate ratio, the average fecal recovery (FR) of TiO2 was higher than FR of Cr2O3, and both FR were more than unity. With internal markers, acetyl bromide lignin and cutin FR were lower than unity, and average FR for indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) was 1.5. The FR was unaffected by the fecal sampling procedure and appears to be an intrinsic property of each molecule and how it interacts with digesta. Of the 2 external markers, only Cr2O3 produced accurate fecal output (FO) estimates and the same happened to dry matter digestibility (DMD) when iNDF and iADF were used. Estimates for DMD and FO were affected by sampling procedure; 72-h bulk [sub-sample from total feces collection (TFC)] sampling consistently produced accurate results. The grab (sub-samples taken at specific times during the day) sampling procedures were accurate when using either of the indigestible fibers (iNDF or iADF) to estimate DMD. However, grab sampling procedures can only be recommended when concomitant TFC is performed on at least one animal per treatment to determine FR. Under these conditions, Cr2O3 is a suitable marker for estimating FO, and iNDF and iADF are adequate for estimating DMD. Moreover, the Cr2O3+iADF marker pair produces accurate dry matter intake estimates and deserves further attention in ruminant nutrition studies. The method of dosing the external markers is extremely important and greatly affects and determines results. Whichever the method, it must allow the animals to display normal feeding behavior and not affect performance. The grab sampling procedures can replace TFC (once FR is established), which may open new possibilities for pasture-based or collectively housed animals.

Dietas para ovinos contendo capim elefante maduro e raspa de mandioca: estimativa de digestibilidade aparente por meio de indicadores

Objetivou-se a avaliar a estimativa da digestibilidade aparente (DA) em dietas para ovinos por meio dos indicadores internos MSi (matéria seca indigestível), FDNi (fibra em detergente neutro indigestível) e FDAi (fibra em detergente ácido indigestível) e o indicador externo LIPE®. Utilizou-se oito ovinos machos Santa Inês, castrados, fistulados no rúmen, com peso médio corporal de 50 kg. Os animais receberam dietas a base de raspa de mandioca e do capim elefante maduro (120 dias). Os indicadores internos (MSi, FDNi e FDAi) foram estimados após 288 horas de incubação in situ das amostras de alimentos e fezes. O indicador externo LIPE® foi fornecido na forma de cápsulas na dosagem de 250 mg/animal/dia. Os resultados de DA estimados pelo indicador externo LIPE® diferiram estatisticamente (P0,05) entre o método da coleta total de fezes e os indicadores internos FDNi e FDAi. Os indicadores internos FDNi e FDAi podem ser utilizados em estudos de digestibilidade em ovinos, pois, permitem estimar de forma semelhante ao método da coleta total de fezes.

Dietary protein reduction on microbial protein, amino acids digestibility, and body retention in beef cattle. I. Digestibility sites and ruminal synthesis estimated by purine bases and 15N as markers.

The objectives of this study were to evaluate the effect of reducing dietary CP contents on 1) total and partial nutrient digestion and nitrogen balance and 2) on microbial crude protein (MCP) synthesis and true MCP digestibility in the small intestine obtained with 15N and purine bases (PB) in beef cattle. Eight bulls (4 Nellore and 4 Crossbred Angus × Nellore) cannulated in the rumen and ileum were distributed in duplicated 4 × 4 Latin squares. The diets consisted of increasing CP contents: 100, 120, or 140 g CP/kg DM offered ad libitum, and restricted intake (RI) diet with 120 g CP/kg DM. The experiment lasted four 17-d periods, with 10 d for adaptation to diets and another 7 for data collection. Omasal digesta flow was obtained using Co-EDTA and indigestible NDF (iNDF) as markers, and to estimate ileal digesta flow only iNDF was used. From days 11 to 17 of each experimental period, ruminal infusions of Co-EDTA (5.0 g/d) and 15N (7.03 g of ammonium sulfate enriched with 10% of 15N atoms) were performed. There was no effect of CP contents (linear effect, P = 0.55 and quadratic effect, P = 0.11) on ruminal OM digestibility. Intake of CP linearly increased (P < 0.01) with greater dietary CP. The NH3-N (P < 0.01) and urinary N excretion (P < 0.01) increased in response to dietary CP, whereas retained N increased linearly (P = 0.03). Liquid-associated bacteria (LAB) in the omasum had greater N content (P < 0.05) in relation to the particle-associated bacteria (PAB). There was no difference between LAB and PAB (P = 0.12) for 15N:14N ratio. The 15N:14N ratio was greater (P < 0.01) in RI animals in relation to those fed at voluntary intake. Microbial CP had a quadratic tendency (P = 0.09) in response to CP increase. Microbial efficiency (expressed in relation to apparent ruminally degradable OM and true ruminally degradable OM) had a quadratic tendency (P = 0.07 and P = 0.08, respectively) to CP increasing and was numerically greatest at 120 g CP/kg DM. The adjusted equations for estimating true intestinal digestibility of MCP (Y1) and total CP (Y2) were, respectively, as follows: Y1 =--16.724(SEM = 40.06) + 0.86X(SEM = 0.05) and Y2 = -43.81(SEM = 49.19) + 0.75X(SEM = 0.05). It was concluded that diets with 120 g/kg of CP optimize the microbial synthesis and efficiency and ruminal ash and protein NDF digestibility, resulting in a better use of N compounds in the rumen. The PB technique can be used as an alternative to the 15N to estimate microbial synthesis.

Polyethylene glycol as an indigestible marker to estimate fecal output in dairy cows

The objective of this study was to evaluate the accuracy of fecal output measurements using polyethylene glycol (PEG) as an external marker determined by near-infrared reflectance spectroscopy. In addition, the accuracy of dry matter intake predictions based on fecal output and digestibility estimated using an internal marker [indigestible neutral detergent fiber (iNDF)] was assessed. The experiment was conducted using 6 lactating dairy cows fed 2 different diets. Polyethylene glycol was administered twice daily into the rumen and the diurnal pattern of fecal concentrations and recovery in feces were determined. To evaluate the effects of alternative marker administration and sampling schemes on fecal output estimates, the passage kinetics of PEG in the digestive tract of dairy cows was determined and used for simulation models. The results indicate that PEG was completely recovered in feces and, thus, fecal output was accurately estimated using PEG. Good agreement between measured and predicted dry matter intake (standard error of prediction = 0.86 kg/d, R2 = 0.81) indicates good potential to determine feed intake using PEG in combination with iNDF. The precision of cow-specific digestibility estimates based on iNDF was unsatisfactory, but for a group of cows iNDF provided an accurate estimate of dry matter digestibility. The current study indicated that, to overcome inherent day-to-day variation in feed intake and fecal output, the minimum of 4 fecal spot samples should be collected over 4 d. Preferably, these samples should be distributed evenly over the 12-h marker administration interval to compensate for the circadian variation in fecal PEG concentrations.

Technical note: Near infrared reflectance spectroscopy to predict fecal indigestible neutral detergent fiber for dairy cows

In vitro and in situ procedures performed to estimate indigestible neutral detergent fiber (iNDF) in forage or fecal samples are time consuming, costly, and limited by intrinsic factors. In contrast, near infrared reflectance spectroscopy (NIRS) has become widely recognized as a valuable tool for accurately determining chemical composition and digestibility parameters of forages. The aim of this study was to build NIRS calibrations and equations for fecal iNDF. In total, 1,281 fecal samples were collected to build a calibration data set, but only 301 were used to develop equations. Once dried, samples were ground and chemically analyzed for crude protein, ash, amylase and sodium sulfite-treated NDF corrected for ash residue (aNDFom), acid detergent fiber, acid detergent lignin, and in vitro digestion at 240 h to estimate iNDF (uNDF240). Each fecal sample was scanned using a NIRSystem 6500 instrument (Perstorp Analytical Inc., Silver Spring, MD). Spectra selection was performed, resulting in 301 sample spectra used to develop regression equations with good accuracy and low standard error of prediction. The standard error of calibration (SEC), cross validation (SECV), and coefficients of determination for calibration (R2) and for cross validation (1 - VR, where VR = variance ratio) were used to evaluate calibration and validation results. Moreover, the ratio performance deviation (RPD) and ratio of the range of the original data to SECV (range/SECV; range error ratio, RER) were also used to evaluate calibration and equation performance. Calibration data obtained on fiber fractions aNDFom (R2 = 0.92, 1 - VR = 0.87, SEC = 1.48, SECV = 1.89, RPD = 2.80, and RER = 20.19), uNDF240 (R2 = 0.92, 1 - VR = 0.86, SEC = 1.65, SECV = 2.24, RPD = 2.57, and RER = 14.30), and in vitro rumen aNDFom digestibility at 240 h (R2 = 0.90, 1 - VR = 0.85, SEC = 2.68, SECV = 3.43, RPD = 2.53, and RER = 14.0) indicated the predictive equations had good predictive value.

Potential nitrogen to energy synchronization, rumen degradation kinetics, and intestinal digestibility of blend pelleted products of new co-products from bio-fuel processing, pulse screenings and lignosulfonate compound in dairy cows

Recently, a new co-product (carinata meal) has been emerged from the bio-fuel processing industry, which nutritional value has not been yet fully understood and evaluated nor even registered as a feed for ruminants. So, it becomes an urgent need to explore the potential of this new coproduct in livestock feeds when blend with other feedstuffs. The aim of this project was to develop and test blend pelleted products based on combination of new and conventional co-products from bio-fuel (carinata meal) and bio-oil (canola meal) processing, pea screenings and lignosulfonate compound (feed additive) at different levels in order to efficiently utilize the new co-product and optimize various nutrient supply in dairy cows. The eight blend pelleted products were: BPP1: lignosulfonate 0.0% + carinata meal 50.0% + pea screenings 50.0%; BPP2: lignosulfonate 4.8% + carinata meal 47.6% + pea screenings 47.6%; BPP3: lignosulfonate 0.0% + carinata meal 75.0% + pea screenings 25.0%; BPP4: lignosulfonate 4.8% + carinata meal 71.4% + pea screenings 23.8%; BPP5: lignosulfonate 0.0% + canola meal 50.0% + pea screenings 50.0%; BPP6: lignosulfonate 4.8% + canola meal 47.6% + pea screenings 47.6%; BPP7: lignosulfonate 0.0% + canola meal 75.0% + pea screenings 25.0%; BPP8: lignosulfonate 4.8% + canola meal 71.4% + pea screenings 23.8%. All blended proportions were on dry matter (DM) basis. The results were compared between blend pelleted products based on new co-product carinata meal and conventional co-product canola meal; high and low level of inclusion of co-products (contrarily, low and high level of inclusion of pea screenings); and inclusion and no inclusion of lignosulfonate compound in terms of rumen degradation kinetics, indigestible neutral detergent fiber(NDF) at 288 h (iNDF), hourly effective rumen degradation ratios/potential nitrogen-to energy synchronization and intestinal digestion of rumen undegraded nutrients. The results showed that carinata based pelleted product BPP3 and BPP4 had higher (P < 0.05) level of rumen undegraded protein (BCPDVE: 233 and 239 g/kg DM; RUPNRC: 210 and 216 g/kg DM; respectively) than the other blend pelleted products. The BPP3 also showed higher (P < 0.05) effective degradation of NDF (EDNDF: 64 g/kg DM) than the other blend pelleted products except BPP4 and BPP7 (56 and 61 g/kg DM; respectively). The iNDF was higher in canola based pelleted product BPP7, while the values were ranged from 5.3 to 12.5% DM. Intestinal absorbable feed protein (IADP) was higher in BPP4 (168 g/kg DM) than the other pelleted products except BPP3; however, BPP3 showed higher (P < 0.05) total digestible protein (TDP: 405 g/kg DM) than the other blend pelleted products followed by BPP4 (383 g/kg DM) and BPP7 (370 g/kg DM). In addition, BPP3 showed higher (P < 0.05) total digestible NDF (TDNDF: 139 g/kg DM) than the other blend pelleted products. In summary, the new co-product (carinata meal) based pelleted products (BPP1 to BPP4) contained higher RUP (+56 g/kg DM), lower EDCP (–24 g/kg DM), higher EDNDF (+2.9%), lower iNDF (−4.2% DM), higher IADP (+55.7 g/kg DM) and higher TDP (+32 g/kg DM) than canola based pelleted products (BPP6 to BPP8). Therefore, the result of this study suggest that the Carinata meal based pelleted products BPP3 and BPP4 can be used as high quality protein supplements for dairy cows.

Effects of replacement of late-harvested grass silage and barley with early-harvested silage on ruminal digestion efficiency in lactating dairy cows

The objective of this experiment was to quantify the effects of graded replacement of late-harvested grass silage and barley with early-harvested silage on nutrient digestion and rumen fermentation. Four experimental diets were fed to 4 multiparous rumen-cannulated Nordic Red cows in 4 × 4 Latin square design with 21-d periods. Dietary treatments consisted of late-cut grass silage (LS) and rolled barley, which was gradually replaced with early-cut grass silage [ES; 0, 33, 67, and 100% of the forage component (ES + LS) of the diet]. With increased proportion of ES in the diet, the proportion of barley decreased from 47.2 to 26.6% on a dry matter basis. Early- and late-cut silages were harvested at 2-wk intervals (predicted concentrations of metabolizable energy 11.0 and 9.7 MJ/kg of dry matter). The 4 diets were formulated to support the same milk production. Nutrient flows were quantified using omasal sampling technique applying the triple-marker method (Cr, Yb, and indigestible neutral detergent fiber) and 15N as a microbial marker. Feed intake decreased with graded replacement of LS and barley with ES, but milk production was not influenced by diet. Digestibility of nutrients improved with graded addition of ES in the diet with the greatest difference observed in digestibility of neutral detergent fiber (NDF) and potentially digestible NDF (pdNDF). The results suggested that improved cell wall digestibility with graded level of ES in the diet was partly related to higher intrinsic digestibility of ES than LS, and partly due to negative associative effects with an increased proportion of LS and barley in the diet. Efficiency of microbial N synthesis was not influenced by the diet, but ruminal protein degradability increased with ES in the diet. Rumen fermentation pattern was not affected by the diet despite large difference in the profile of dietary carbohydrates. Rumen pool size of NDF and pdNDF, and ruminal turnover time of NDF decreased with graded addition of ES in the diet, whereas digestion rate of pdNDF improved. The results of this study indicate that increased CH4 yield in a parallel production study with graded addition of ES in the diet were more related to greater ruminal and total digestibility of organic matter than to the changes in rumen fermentation pattern.

The effects of feeding a partial mixed ration plus a top-dress before feeding on milk production and the daily rhythm of feed intake and plasma hormones and metabolites in dairy cows

Total mixed rations (TMR) are commonly fed to dairy cows with a goal of reducing ruminal acidosis, but the daily pattern of feed intake has major implication for rumen fermentation and postabsorptive physiology. Generally there is low feed intake during the overnight period and high intake after feeding and during the afternoon. The objective of this study was to determine if feeding a partial mixed ration (PMR) plus a high starch top-dress before feeding of the PMR would improve production and modify key daily rhythms. The top-dress was fed before feeding as ruminal starch is at a nadir and amylolytic capacity is expected to be low. Ten Holstein cows were used in a crossover design with 14-d periods. Cows were housed in tie stalls with feed tubs and feed weight was recorded every 10 s for observation of feeding behavior. Treatments were a control TMR fed once per day at 0900 h or a partial mixed ration plus a top-dress (PMR+TD). The top-dress was fed at 9.5% of DMI offered at 0800 h and contained steamflaked corn, canola meal, and nonprotein nitrogen. No interaction was observed between treatment and milking time. Milk yield tended to be decreased 1.1 kg and milk fat yield was decreased 70 g/d by PMR+TD. Milk fat preformed fatty acids were decreased and no effect was observed of treatment on trans-10 C18:1 or other indicators of biohydrogenation-induced milk fat depression. No effect was observed of treatment on meal parameters including meal size and number. The PMR+TD increased total-tract neutral detergent fiber (NDF) digestibility by 1.2 percentage points. Treatment changed the daily pattern of fecal NDF and indigestible NDF, indicating changes in rumen function over the day. No effect was observed on plasma glucose, but the amplitude of the daily rhythm of insulin was increased by PMR+TD. The PMR+TD also increased plasma nonesterified fatty acids and decreased blood urea nitrogen across the day. Core body temperature is entrained by the central biological clock and its phase was advanced 42 min and its amplitude slightly increased by PMR+TD. In conclusion, the top-dress appears to have modified the central circadian rhythm and plasma insulin and blood urea nitrogen. This demonstrates that timing of feeding can be used to manage daily rhythms of the dairy cow, although the optimal timing requires further investigation.

Short communication: The effects of morning compared with evening feed delivery in lactating dairy cows during the summer

Delivering fresh feed in the evening is a management strategy sometimes used during periods of heat stress, but previous experiments have observed that night feeding increased feed intake during the 2 h after feeding and did not change intake during the overnight period. The objective of this study was to determine the effect of night feeding on daily rhythms of the dairy cow during the summer season. Twelve Holstein cows were used in a crossover design with 14-d periods. An automated system recorded the timing of feed intake over the last 7 d of each period. Treatments were ad libitum feeding with fresh feed delivery 1×/d at 0830 h or 2030 h. Milk yield and composition were not changed by treatment, but night feeding decreased the concentration of preformed fatty acids in milk fat. Night feeding decreased feed intake 1.7 kg/d, and decreased total-tract dry matter and neutral detergent fiber (NDF) digestibility by 0.7 and 0.8 percentage units, respectively. The amount of feed consumed in the first 2 h after feeding was 64% greater with night feeding, but intake did not differ between treatments during the night or early afternoon. A treatment by time of day interaction was observed for fecal NDF and indigestible NDF concentration and plasma glucose, insulin, and urea concentrations. Night feeding resulted in an increase in plasma insulin after feeding, which decreased plasma glucose. The daily rhythm of core body temperature was entrained by treatment, with the phase shifted and the amplitude decreased by night feeding indicating alteration of the central clock. In conclusion, feeding cows once per day in the evening during the summer caused a similar increase in feed intake and plasma insulin after feeding as previously observed during non-heat stress seasons. Night feeding also decreased intake and total-tract digestibility. The daily pattern of feed intake and other behaviors should be considered before initiation of night feeding.

Sugarcane bagasse as exclusive roughage for dairy cows in smallholder livestock system.

ObjectiveThe study aimed to evaluate sugarcane bagasse as roughage in lactating cow on feed intake, digestibility, ingestive behavior, milk production and composition, and microbial protein synthesis.MethodsTen Girolando cows at initial body weight of 450±25.6 kg and at 143.7±30.7 days in milk were assigned in two 5×5 Latin square designs. Five 21-day experimental periods were adopted (1° to 14-day: diets adaptation period; 15° to 21-day: data collection and sampling period). The diets consisted of four different levels of sugarcane bagasse (45%, 50%, 55%, and 60%) and a control diet, commonly adopted in the region, based on spineless cactus (25% sugarcane bagasse), formulated to meet 12 kg/d milk yield.ResultsThe dry matter (DM), organic matter (OM), and total digestible nutrients intakes and DM and OM digestibilities observed for 45% and 50% bagasse inclusion were similar to control diet, while that 55% and 60% bagasse inclusion were lower. Cows fed control diet, and bagasse diets of 45%, and 50% levels had the nutritional requirements attended, that guaranteed 12 kg/d of milk yield. The crude protein intake and digestibility of cows fed 45%, 50%, and 55% of bagasse inclusion were similar to control diet. The neutral detergent fiber (NDF) intake and digestibility differ for all bagasse diets related to control diet, while the non-fiber carbohydrates intake and digestibility for cows fed 45% of bagasse were similar for control diet. The intakes and digestibilities of nutrients decreased linearly in function of bagasse inclusion; NDF and indigestible NDF intakes did not vary. The ruminating time, feeding and rumination efficiency, microbial protein synthesis and milk yield decreased linearly with sugarcane bagasse inclusion.ConclusionSugarcane bagasse decreases milk production; however, its inclusion level in between 45% to 50% associated to concentrate could replace diets based on spineless cactus for crossbred dairy cow's producing 12 kg/d of milk.

Evaluation of internal markers as determinants of fecal dry matter output and digestibility in feedlot sheep

This study aimed to evaluate the fecal recovery of the internal markers indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF), and indigestible acid detergent fiber (iADF) and to compare them as estimators of the fecal dry matter output (FDMO) and digestibility of the dry matter (DM) with values observed in total feces collection in trials with feedlot sheep. For this, 82 sampling units from two experiments were evaluated. Internal markers were quantified in the feed supplied, orts, and feces. The recovery of the markers was determined by the comparison of the amount of marker consumed and excreted. Fecal dry matter output and digestibility were determined based on the amount of marker consumed and on its concentration in the feces through individual collections directly from the rectum of the animals, with values compared with those observed in the total feces collection from orthogonal contrasts. The mean bias, the concordance correlation coefficient, and the mean squared prediction error were also evaluated. For all statistical procedures, the critical level of probability for type-I error was fixed at 0.05. The marker iDM showed complete fecal recovery (100.03%) and was efficient in estimating FDMO (482.9 g day-1) and DM digestibility (542 g kg-1) as compared with the values observed in the total feces collection (454.7 g day-1 and 561 g kg-1 for FDMO and DM digestibility, respectively). The iNDF and iADF markers did not show complete recovery in the feces and were not effective in the estimate of FDMO and DM digestibility.

Acceptability by Girolando heifers and nutritional value of erect prickly pear stored for different periods

Abstract: The objective of this work was to evaluate the nutritional value of erect prickly pear (EPP) (Opuntia stricta), stored for different periods, and its acceptability by Girolando heifers. Five heifers were distributed in a 5x5 Latin square. The treatments were as follows: EPP without storage time; stored EPP for three post-harvest periods - 7, 14, and 21 days; and cochineal nopal cactus (CNC) (Nopalea cochenillifera) without storage time, as a control treatment. The diets offered to the animals were composed of cacti of the different treatments, as well as sugarcane silage and soybean meal, and were evaluated for their chemical composition, intake, and nutrient digestibility. The ingestive behavior of the heifers was evaluated. The cactus EPP did not differ from the CNC for organic matter, crude protein, neutral detergent fiber corrected for ash and protein, nonfibrous carbohydrates, total carbohydrates, ether extract, and mineral matter; however, EPP stored for different periods differed from CNC for dry matter and indigestible neutral detergent fiber. Heifer weights varied 1.35 kg with the low inclusion of concentrate in the diet. The cactus erect prickly pear maintains constant both its nutritional value and its acceptability to Girolando heifers, after storage for different periods up to 21 days.

Defoliation height and potassium fertilization of Mulato II grass

A pot trial in greenhouse conditions was carried out to identify at which defoliation height Mulato II grass (Urochloa hibrida cv. Mulato II) should be managed and to determine whether potassium fertilization is necessary in soil with high potassium content. The experiment was carried out in a greenhouse in a randomized block design with six treatments and five replications. Treatments consisted of a 3 × 2 factorial arrangement, with three defoliation heights (50, 65, and 80 cm) and with or without potassium maintenance fertilization. The production characteristics and chemical composition of the forage plant were evaluated. There was no interaction effect between defoliation height and fertilization with or without potassium on any of the analyzed variables, except for mineral content in Mulato II grass. The tillers and leaves number, shoot dry matter, leaf+sheath, root system, and residue were influenced by defoliation heights and potassium fertilization, except for the leaf blades and root dry matter, leaf blade/stem+sheath ratio, and leaves number, which were not influenced by potassium fertilization. Higher shoot dry matter was observed at the heights of 65 and 80 cm; however, comparing these two heights, leaf + sheath dry matter was lower at 65 cm. Regarding the grass’s chemical composition, there was an increase in neutral and acid detergent fiber and indigestible neutral detergent fiber contents as the defoliation height increased, which resulted in lower production of potentially digestible dry matter, which can compromise the potential use of the forage by animals. Among evaluated treatments, Mulato II grass defoliation is recommended for a maximum height of 65 cm. Potassium fertilization increases the yield and the potentially digestible dry matter content of Mulato II grass, even when cultivated in soil with high potassium content.

Defoliation height and potassium fertilization of Mulato II grass

A pot trial in greenhouse conditions was carried out to identify at which defoliation height Mulato II grass (Urochloa hibrida cv. Mulato II) should be managed and to determine whether potassium fertilization is necessary in soil with high potassium content. The experiment was carried out in a greenhouse in a randomized block design with six treatments and five replications. Treatments consisted of a 3 × 2 factorial arrangement, with three defoliation heights (50, 65, and 80 cm) and with or without potassium maintenance fertilization. The production characteristics and chemical composition of the forage plant were evaluated. There was no interaction effect between defoliation height and fertilization with or without potassium on any of the analyzed variables, except for mineral content in Mulato II grass. The tillers and leaves number, shoot dry matter, leaf+sheath, root system, and residue were influenced by defoliation heights and potassium fertilization, except for the leaf blades and root dry matter, leaf blade/stem+sheath ratio, and leaves number, which were not influenced by potassium fertilization. Higher shoot dry matter was observed at the heights of 65 and 80 cm; however, comparing these two heights, leaf + sheath dry matter was lower at 65 cm. Regarding the grass’s chemical composition, there was an increase in neutral and acid detergent fiber and indigestible neutral detergent fiber contents as the defoliation height increased, which resulted in lower production of potentially digestible dry matter, which can compromise the potential use of the forage by animals. Among evaluated treatments, Mulato II grass defoliation is recommended for a maximum height of 65 cm. Potassium fertilization increases the yield and the potentially digestible dry matter content of Mulato II grass, even when cultivated in soil with high potassium content.

Effect of dietary supplementation with heat-treated canola meal on ruminal nutrient metabolism in lactating dairy cows

An experiment was conducted to quantify the effects of incremental levels of heat-moisture-treated canola meal (TCM) fed to dairy cows on the relationship between ruminal nutrient digestion and milk production. Experimental diets were fed to 4 multiparous rumen-cannulated Nordic Red cows, averaging (mean ± standard deviation) 681 ± 54.8 kg of body weight, 111 ± 16 d in milk, and 29.1 ± 9.1 kg of milk/d at the start of the study, in a Latin square design with four 21-d periods. The 4 experimental dietary treatments consisted of a basal diet of grass silage and crimped barley, and 3 diets in which the crimped barley was replaced with TCM, giving 3 incremental levels of protein supplementation. Nutrient flow was quantified by the omasal sampling technique using 3 markers (Cr, Yb, and indigestible neutral detergent fiber). Continuous infusion of 15N was used to label bacterial crude protein. Additionally, ruminal sampling and evacuations and measurements of total-tract digestibility were conducted. The experimental diets provided 132, 148, 164, and 180 g of crude protein/kg of dry matter. The increased level of TCM linearly increased dry matter intake from 15.1 to 16.6 kg/d and energy-corrected milk yield from 21.0 to 25.6 kg/d. The increased proportion of TCM when substituting barley with TCM was associated with greater total-tract digestibility of neutral detergent fiber and potentially digestible neutral detergent fiber, which could be explained by increased digestion rate of potentially digestible neutral detergent fiber. Omasal flow of nonammonia N naturally increased with greater dietary TCM inclusion, but the increased intestinal supply of rumen-undegradable protein was partly offset by diminished microbial protein synthesis when feeding more TCM. This was also reflected in a decreased proportion of milk protein from ruminal bacterial protein when TCM supplementation increased.