Indigenous Cattle Research Articles

Genome-wide signature of positive selection in Ethiopian indigenous and European beef cattle breeds

BackgroundDespite the availability of genome-wide SNPs to uncover the origin and divergence of Ethiopian cattle population, knowledge regarding their genetic adaptability and divergence remain limited. To investigate signature of selection, three Ethiopian cattle breeds were genotyped with 80 K SNP array and three European beef cattle breeds were also used for comparison purposes. ResultsAcross Ethiopian cattle populations, the mean observed and expected heterozygosity were 0.403 and 0.400, respectively, and for European beef cattle breeds observed and expected heterozygosity were 0.25 and 0.26 respectively. PCA and NJ-tree grouped the study cattle according to their breed group with close clustering of Ethiopian cattle breeds. The top 1% values were considered to delimit genomic regions under positive selection. Some of the candidate genes involved in biological processes and pathways linked to meat quality attributes. Furthermore, some of the candidate genes associated with tropical adaptation like heat tolerance and resistance to disease. ConclusionThe genetic divergence of Ethiopian breeds from European beef breeds coincides with existing knowledge that European cattle breeds considered in this study are artificially selected for beef traits, while Ethiopian indigenous breeds are naturally selected.

Serological Prevalence of Crimean-Congo Hemorrhagic Fever Virus Infection in Small Ruminants and Cattle in The Gambia.

Crimean-Congo hemorrhagic fever virus (CCHFV) is a widely distributed tickborne zoonotic agent that infects a variety of host species. There is a lack of information on the true geographic distribution of the prevalence and risk of CCHFV in West Africa. A countrywide cross-sectional study involving 1413 extensively managed indigenous small ruminants and cattle at livestock sales markets and in village herds, respectively, was carried out in The Gambia. In sheep, an overall anti-CCHFV antibody prevalence of 18.9% (95% CI: 15.5-22.8%), goats 9.0% (95% CI: 6.7-11.7%), and cattle 59.9% (95% CI: 54.9-64.7%) was detected. Significant variation (p < 0.05) in the prevalence of anti-CCHFV antibodies at sites in the five administrative regions (sheep: 4.8-25.9%; goats: 1.8-17.1%) and three agroecological zones (sheep: 8.9-32.9%; goats: 4.1-18.0%) was also observed. Comparatively, higher anti-CCHFV antibody prevalence was detected in cattle (33.3-84.0%) compared to small ruminants (1.8-8.1%). This study represents the first countrywide investigation of the seroprevalence of CCHFV in The Gambia, and the results suggest potential circulation and endemicity of the virus in the country. These data provide critical information vital to the development of informed policies for the surveillance, diagnosis, and control of CCFHV infection in The Gambia and the region.

Management practices, reproductive performances, and production constraints of indigenous cattle in north-western parts of Ethiopia

Management practices, reproductive performances, and production constraints of indigenous cattle in north-western parts of Ethiopia

Prevailing Calf Mortality and its Pattern in Indigenous Cattle in Operational Area of Dairy Vigyan Kendra, Vejalpur, Gujarat

The objective of this study was to assess the prevailing indigenous calf mortality &amp; its pattern in the operational area of Dairy Vigyan Kendra (DVK), Vejalpur, Gujarat, India. Using the simple random sampling method, 300 indigenous cattle owners from 6 villages each from 10 talukas were selected for the study from August to December 2021. The data were collected based on the pre-tested structured interview schedule. Overall indigenous calf mortality was observed 27.90 % in the study area. There was a higher mortality rate in male calves (53.43%) than in female indigenous calves (11.97%) due to limited attention towards the male calf. The mortality rate was recorded higher during the first three months of age (76.42%) and during the summer (50.00%). The major reason of calf mortality was diarrhoea or dysentery. Most indigenous cattle owners had not adopted the recommended calf management practices. Farmers urgently need extension services on scientific calf rearing practices such as cutting navel cord with sterilized object, providing colostrum and milk as per body weight, on-time colostrum feeding, provision of milk replacer, calf starter, salt, mineral mixture, deworming, and HS &amp; FMD vaccination.

Comparison of neurological examination/clinical signs of pe-ripheral and central vestibular disorders in Hanwoo (Korean indigenous cattle)

Comparison of neurological examination/clinical signs of pe-ripheral and central vestibular disorders in Hanwoo (Korean indigenous cattle)

Genomic variability and population structure of six Colombian cattle breeds

Analyses of the genetic diversity of indigenous cattle are essential for implementing conservation programs, promoting their sustainable use and maintaining productive advantages offered by these breeds in local conditions. The aim of this study was to investigate the genetic diversity and population structure of six Colombian cattle breeds: Blanco Orejinegro (BON), Costeño con Cuernos (CCC), Romosinuano (ROM), Sanmartinero (SAM), Casanareño (CAS), and Hartón del Valle (HDV). Two additional breed groups were included for comparison: Zebu (CEB) and a crossbreed of Colombian cattle breeds × Zebu. Genetic diversity within breeds was analyzed using expected heterozygosity (He), inbreeding coefficient (f), and runs of homozygosity (ROH). Population structure was assessed using model-based clustering (ADMIXTURE) and principal components analysis (PCA). Zebu cattle showed the lowest genetic diversity (He = 0.240). Breeds with the highest genetic diversity level were HDV and BON (He = 0.350 and 0.340, respectively). Inbreeding was lower for Colombian cattle breeds ranging between 0.005 and 0.045. Overall, the largest average genetic distance was found among the group of Colombian cattle breeds and Zebu, while the smallest was found between ROM and CCC. Model-based clustering revealed some level of admixture among HDV and CAS cattle which is consistent with their recent history. The results of the present study provide a useful insight on the genetic structure of Colombian cattle breeds.

Prevalence and Risk Factors Associated with Theileria annulata Infection in Two Bovine Portuguese Autochthonous Breeds.

Tropical Bovine Theileriosis is an important tick-borne disease. This study aims to assess the occurrence of Theileria annulata infection in two indigenous Portuguese cattle breeds. A total of 843 blood samples collected from animals of Alentejana (n = 420) and Mertolenga (n = 423) breeds were analyzed. The detection of Theileria annulata was determined by amplification of a fragment of the merozoite-pyroplasm surface antigen gene with 319 base pairs (bp). The prevalence found (10.8%) is lower than that reported in previous studies (21.3%). A statistically significant difference was found for positivity between breeds (p < 0.05). There is also a higher probability of older animals being positive compared to younger ones (p < 0.05). The region where Mertolenga animals are located is shown to have a significant impact on positivity (p < 0.05). Thus, the development of sustainable T. annulata control strategies and their implementation, adapted to the epidemiological conditions of higher risk, will be extremely important.

Effect of dental age on physico-chemical properties of meat of indigenous beef cattle

The objective of this study was to know the physico-chemical properties of beef in relation to dental age of the animals. This experiment was conducted with five (5) treatments (T1, T2, T3, T4 and T5) where T1 = 0 Permanent incisor, T2 = 2 Permanent incisors, T3 = 4 Permanent incisors, T4 = 6 Permanent incisors, T5 = 8 Permanent incisors having ten (10) replications. From the experiment it is found that colour band of a* (P=0.0008) was significantly higher in the T5 and T2 than others and colour band of c* (P=0.0116) was significantly higher in the T5 than T1, T3 and T4. Dental age maturity of beef cattle did not show an effect on drip-loss of beef LM having the age group of T1 to T5 from the post mortem day 24 hrs to 1 week with an exception of post mortem day 5 where dental maturity has a significant effect on drip loss. Cooking loss at room temperature (RT) to 100 °C at holding time of 40 minutes was significantly higher in T1 and lower in T5. The similar trend was also observed at 100 °C in holding time of 60 minutes and 90 minutes. In both cases, cooking loss was markedly increased (p&lt;0.001) in T1 and decreased (p&lt;0.001) with the advances of dental maturity. Carcass pH 2h were significantly increased gradually with the advances of age T1, T2, T4 and T5; where pH2h, was highest in T3 and lowest in T1. In conclusion, dental age affects colour, drip loss (post mortem day 5), cooking loss and carcass pH 2h of meat of indigenous beef cattle.

Quality and acceptability evaluation of soy-cheese (Tofu) enhanced with meat

The objective of this study was to know the physico-chemical properties of beef in relation to dental age of the animals. This experiment was conducted with five (5) treatments (T1, T2, T3, T4 and T5) where T1 = 0 Permanent incisor, T2 = 2 Permanent incisors, T3 = 4 Permanent incisors, T4 = 6 Permanent incisors, T5 = 8 Permanent incisors having ten (10) replications. From the experiment it is found that colour band of a* (P=0.0008) was significantly higher in the T5 and T2 than others and colour band of c* (P=0.0116) was significantly higher in the T5 than T1, T3 and T4. Dental age maturity of beef cattle did not show an effect on drip-loss of beef LM having the age group of T1 to T5 from the post mortem day 24 hrs to 1 week with an exception of post mortem day 5 where dental maturity has a significant effect on drip loss. Cooking loss at room temperature (RT) to 100 °C at holding time of 40 minutes was significantly higher in T1 and lower in T5. The similar trend was also observed at 100 °C in holding time of 60 minutes and 90 minutes. In both cases, cooking loss was markedly increased (p&lt;0.001) in T1 and decreased (p&lt;0.001) with the advances of dental maturity. Carcass pH 2h were significantly increased gradually with the advances of age T1, T2, T4 and T5; where pH2h, was highest in T3 and lowest in T1. In conclusion, dental age affects colour, drip loss (post mortem day 5), cooking loss and carcass pH 2h of meat of indigenous beef cattle.

Evaluation of nutritional quality of fat obtained from different breeds of cow and buffalo with respect to fatty acids and in vitro digestibility

A comparative fatty acid analysis of raw milk and during in vitro digestion of milk from different breeds of cow (Gir, Sahiwal, Tharparkar, and Karan fries) and buffalo (Murrah) maintained under similar feeding regimes was conducted. Saturated fatty acids were higher in Murrah and Karan Fries, whereas monounsaturated fatty acids were higher in Gir, Sahiwal and Tharparkar. Based on the fatty acid profile, atherogenic, thrombogenic index and hypercholesterolemic saturated fatty acids were lower, and desired fatty acids were higher in milk from indigenous breeds than in crossbred and Murrah. The size of milk fat globules was also lower in indigenous cattle breeds, which has allowed for faster in vitro digestibility of milk fat.

Potential status of A1 and A2 variants of bovine beta-casein gene in milk samples of Indian cattle breeds

This study aimed to determine the polymorphism in 7th exon of beta-casein gene (CSN2) gene in seven domestic (Kosali, Tharparkar, Gangatiri, Sahiwal, Gir, Khariar, Motu) and two exotic cattle breeds (Jersey and Holstein-Friesian). Genomic DNA was extracted from 1000 milk samples, and the C > A polymorphism in CSN2 was determined using the tetra-primer amplification refractory mutation system-polymerase chain reaction method. In all Indigenous cattle breeds, the mean frequency of A1A2 and A2A2 genotypes was 0.19 and 0.80, respectively. The A1A1 genotype was absent in all seven domestic cattle breeds. The frequency of the A2A2 genotype was highest in the Gir breed (0.93). However, the Sahiwal, Tharparkar, and Motu breeds also had a higher frequency of A2A2 genotype compared to other breeds. In contrast, Gangatiri breed of India showed lowest frequency of A2A2 genotype. The mean A1 and A2 allele frequency was 0.09 and 0.91, respectively. In exotic breeds, the mean frequencies of the A1A1, A1A2, and A2A2 genotypes were 0.42, 0.55, and 0.03, respectively. Similarly, the mean A1 and A2 allele frequency was 0.69 and 0.31, respectively. This study suggests the high potential of Gir, Sahiwal, Tharparkar, and Motu cattle for A2 milk production since they carry a favorable A2 genotype.

Whole Genome Resequencing Reveals Genetic Diversity and Selection Signatures of Ethiopian Indigenous Cattle Adapted to Local Environments

Cattle are among the most important domesticated bovid species in the world, of which Ethiopia possesses large populations adapted to different agro-ecologies and production systems. Though several molecular population genetic studies have been done on Ethiopian indigenous cattle, genomic diversity and selection signatures pertinent to adaptation to the different local environments have yet to be comprehensively characterized. Here, the whole genome sequences of 151 samples from 14 Ethiopian indigenous cattle populations were analyzed to assess genomic diversity and differentiation as well as signatures of positive selection (using Hp, iHS, FST, and XP-CLR) in comparison to Sudanese zebu, Asian zebu, Ankole, and African and European taurine cattle. High genomic differentiation was observed between Ethiopian and non-Ethiopian cattle populations, while low genomic differentiation and inbreeding were present between and within Ethiopian cattle populations. Sixteen genome regions overlapping with 40 candidate genes were commonly identified by at least three genome scan methods. High frequencies of missense SNPs in ITPR2, CHADL, GNAS, STING1, and KIT genes with high haplotype differentiations were observed in Ethiopian cattle compared to non-Ethiopian cattle. The candidate genes were significantly associated with several biological functions and molecular pathways responsible for nutrient metabolism, skeletal development, immune response, reproduction, water balance, coat color pigmentation, and circulatory homeostasis. Our results provide new insights into the adaptation of the Ethiopian indigenous cattle to the country’s diverse environments.

Current Status and Conservation Management of Farm Animal Genetic Resources in Bhutan

Farm Animal Genetic Resources (FAnGR) ensures food security and maintains genetic diversity. The efforts to conserve FAnGR are minimal in Bhutan. In the pursuit of increasing livestock productivity, farmers are rearing livestock that narrows the range of genetic diversity. This review attempts to summarize the status of FAnGR and the efforts to conserve them. Some unique livestock breeds in Bhutan are Nublang (cattle breed), Yak, Saphak (pig breed), Yuta (horse breed), Merak-Saktenpa (horse breed), and Belochem (chicken breed). There was a drop in the yak, buffalo, equine, pig, sheep, and goat populations. Both in situ and ex situ conservation measures are in place for some of the breeds and strains (e.g., Nublang and traditional chicken). Conservation efforts are limited to the government, but other individuals, stakeholders and non-government organizations must play an increasing role in preserving genetic diversity. It is pertinent that Bhutan comes up with a policy framework to conserve its indigenous cattle.

Genomic adaptation to extreme climate conditions in beef cattle as a consequence of cross-breeding program

BackgroundUnderstanding the evolutionary forces related to climate changes that have been shaped genetic variation within species has long been a fundamental pursuit in biology. In this study, we generated whole-genome sequence (WGS) data from 65 cross-bred and 45 Mongolian cattle. Together with 62 whole-genome sequences from world-wide cattle populations, we estimated the genetic diversity and population genetic structure of cattle populations. In addition, we performed comparative population genomics analyses to explore the genetic basis underlying variation in the adaptation to cold climate and immune response in cross-bred cattle located in the cold region of China. To elucidate genomic signatures that underlie adaptation to cold climate, we performed three statistical measurements, fixation index (FST), log2 nucleotide diversity (θπ ratio) and cross population composite likelihood ratio (XP-CLR), and further investigated the results to identify genomic regions under selection for cold adaptation and immune response-related traits.ResultsBy generating WGS data, we investigated the population genetic structure and phylogenetic relationship of studied cattle populations. The results revealed clustering of cattle groups in agreement with their geographic distribution. We detected noticeable genetic diversity between indigenous cattle ecotypes and commercial populations. Analysis of population structure demonstrated evidence of shared genetic ancestry between studied cross-bred population and both Red-Angus and Mongolian breeds. Among all studied cattle populations, the highest and lowest levels of linkage disequilibrium (LD) per Kb were detected in Holstein and Rashoki populations (ranged from ~ 0.54 to 0.73, respectively). Our search for potential genomic regions under selection in cross-bred cattle revealed several candidate genes related with immune response and cold shock protein on multiple chromosomes. We identified some adaptive introgression genes with greater than expected contributions from Mongolian ancestry into Molgolian x Red Angus composites such as TRPM8, NMUR1, PRKAA2, SMTNL2 and OXR1 that are involved in energy metabolism and metabolic homeostasis. In addition, we detected some candidate genes probably associated with immune response-related traits.ConclusionThe study identified candidate genes involved in responses to cold adaptation and immune response in cross-bred cattle, including new genes or gene pathways putatively involved in these adaptations. The identification of these genes may clarify the molecular basis underlying adaptation to extreme environmental climate and as such they might be used in cattle breeding programs to select more efficient breeds for cold climate regions.

Hemorrhagic disease caused by bovine viral diarrhea virus-2a in Korean Indigenous Cattle: case reports

Bovine viral diarrhea virus (BVDV), which is prevalent worldwide, is one of the most important viral pathogens and causes substantial economic loss to the livestock industry. Despite its importance, BVDV is largely unnoticed in the Republic of Korea (ROK). In this study, we report the case of a steer with BVDV that died suddenly due to severe enteritis. Two 1-year-old Korean indigenous cattle in the same herd presented severe hemorrhagic diarrhea. Case 1 had severe dehydration and died after 3 days, whereas case 2 had anorexia, depression, and severe diarrhea with mucus and blood. Only case 2 was necropsied, and BVDV2a was detected in the tissues of its alimentary tract. Gross lesions, including erosion, ulceration, and extensive hemorrhage, were observed in the digestive tract mucosa. Immunohistochemistry revealed marked positive staining for BVDV2a antigen in the large intestine. This report describes the first case of hemorrhagic disease caused by acute BVDV2a infection, which is characterized by high mortality in Korean indigenous cattle. This study will help establish vaccination and control strategies for BVDV in the ROK.

A Retrospective Study on Dairy Cattle Mortality Patterns in Two Farms of South-eastern Botswana

Generally, high mortalities of dairy cattle due to infectious and non-infectious diseases cause huge economic losses, unprofitability, and low productivity in the dairy industry. The present study aimed at determining the mortality rates, their causes, and risk factors among 1779 cattle at two dairy farms belonging to the Department of Agricultural Research, Botswana. An 8-year retrospective study was conducted using farm records during 2005-2012. Monthly and annual records of the farms were examined regarding the total dairy cattle population, sex, breed, age, cattle deaths, and causes of death. Mortality was calculated from the total cattle population and expressed as a percentage, and it was analyzed with respect to farm, breed, age, sex, year, season, and mortality causes. The overall mortality rate was 8.5%. The semi-intensively managed Farm II, as well as young stock (&lt;12 months old), and males recorded significantly higher mortalities than their counterparts. Dairy crosses of pure exotic and indigenous Tswana cattle had higher mortalities than the Friesians and Jerseys, and the wet season accounted for over 70% of the total deaths. Only two years (2010 and 2012) out of the 8-year study period had a mortality rate &lt; 5%. Notably, 28.1 % of mortalities with a known cause were due to heartwater disease (n = 57), but most deaths (62.3%) were due to unknown causes. In conclusion, to improve farm herd health and husbandry practices, more efforts should be devoted to preventing heartwater and mortalities in young stock and male animals, particularly during the hot-wet season.

Exploring haplotype block structure, runs of homozygosity, and effective population size among dairy cattle breeds of India

The present study aimed to explore haplotype structure, runs of homozygosity (ROH), effective population size and persistence of gametic phase among three indigenous dairy cattle breeds, viz., Sahiwal (n = 19), Tharparkar (n = 17), and Gir (n = 16) by using BovineHD single nucleotide polymorphism (SNP) genotyping assay. The filtered SNPs after quality control ranged from 44% in Sahiwal to 53% in Gir. The highest number of haplotype blocks was observed in Tharparkar (15,640) and the lowest in Sahiwal (8027) spanning 17.3% and 7.8% of genome, respectively. The average block length was found close to 26kb which suggests that multiple recombination events fragmented the ancestral haplotypes into smaller sizes. Gir cattle had the largest number of runs of homozygosity (ROH) regions (1762) followed by Tharparkar (1528) and Sahiwal (1138). Without pedigree information, inbreeding coefficients estimated from ROH (FROH) revealed that Gir had the highest FROH (0.099) proposing more inbreeding rate in this population. Effective population size (Ne) decreased slowly over the last 60 generations and at 13 generations ago; Ne was estimated as 70 for all the three dairy breeds. The highest gametic phase correlation (r = 0.78) was observed for Sahiwal and Tharparkar breed pair suggesting formulation of multi-breed reference population for successful implementation of genomic selection among dairy breeds. The decline in effective population size among native Indian cattle breeds may help in formulating strategies for conservation and genetic improvement of native germplasm for future use.

Successful treatment of idiopathic tetanus using metronidazole, magnesium, and acepromazine in Hanwoo (Korean indigenous cattle) yearling bull.

Bovine tetanus is a serious infectious disease of the central nervous system caused by the exotoxin produced by Clostridium tetani and is characterized by persistent tension and spasm of the rhabdomyocytes. Currently, many studies have focused on diagnosing tetanus; however, only a few studies on treatment methods have been conducted. Therefore, cattle with tetanus have been treated using symptomatic therapy. In this case, severe muscle spasticity and spasms were observed in a 9-month-old Hanwoo (Korean indigenous cattle) bull, and aspartate aminotransferase and creatine kinase levels were increased in serum biochemical tests. Clinically, bovine tetanus was strongly suspected, and metronidazole was administered orally for 5 days. To treat the intensifying bloat, a temporary rumenostomy was performed on the third day of onset, and the toxin gene (tetanospasmin) of C. tetani was amplified by polymerase chain reaction analysis from the collected ruminal fluid. Magnesium and sedatives (acepromazine) were administered for 7 days to treat muscle spasticity and spasms. Muscle spasticity and spasm markedly improved, and the bull stood up from the lateral recumbent position. On the 17th day after onset, all tetanus-related symptoms resolved and a normal diet was started. Our findings demonstrated that treatment with metronidazole, magnesium, and acepromazine was effective in the bull with tetanus.

Synergies of feed, management trainings, and genetics on milk production of dairy cows in the tropics: The case of Ethiopian smallholder farmers

Efforts made so far to increase milk production of indigenous cattle by upgrading with exotic genotypes through crossbreeding under smallholder farmer conditions in Ethiopia have resulted in limited improvement. This study was conducted to determine if combining improved feed and management-related trainings with Holstein genetics will synergistically increase the milk production of dairy cows, and to examine the degree to which the increase or improvement is related to the level of exotic genetics involved. A total of 96 smallholder dairy farmers were purposively selected and allocated to one of three treatments reflecting differing degrees of capacity building support: (1) no training and no material support (Control); (2) training provided by another project (PAID) but without material support; (3) enhanced training plus material support (Feed-Mgt). Sixteen extension development agents and eight livestock experts were selected to provide training and weekly on-farm data collection, monitoring, technical support, and messaging reinforcement visits. Enhanced training addressed a broad range of topics related to feed and feeding, improved forage production, milk production and handling, construction and use of improved dairy housing, disease control, and dairy farm record keeping. Material support was in the form of formulated dairy concentrate feed and improved forage planting materials (seeds/seedlings). Data collection included variables describing animal performance, management practice adoption, and milk and butter quality. Tail hair samples for genetic profiling of breed makeup were also collected from all cows in the study. Results showed that there was a very significant effect of treatment (P &amp;lt; 0.001) on milk production, as well as treatment by week interaction (P = 0.034), with the effect of treatment markedly increasing over time (P = 0.032). That is, cows in Feed-Mgt group had up to 26.6% greater milk production compared to those in the Control group. Region and region by treatment interaction have no effects. Cows used in the experiment had varying proportions of Holstein-Friesian genome that ranged between 0 and 100%, suggesting prevalence of indiscriminate crossbreeding. Cows with a high proportion of Holstein genetics in treatment 3 produced more milk as compared to those in control group. Besides, supplemental feed improved body condition, reduced number of services per conception and resulted in higher lactation length of cows on treatment 3 compared with those on treatments 1 and 2 (P &amp;lt; 0.001). Overall, our findings suggest that an integrated approach of improved feed, feeding practices, and management training for smallholder dairy that goes beyond the improvement of genetics will increase milk production, improve milk quality and body condition, and resulted in higher length of lactation.

Hemato-biochemical profile of indigenous Badri cattle of Uttarakhand

Badri cattle is the first registered cattle breed of Uttarakhand and reared in the Kumaon and Garhwal regions. The study was undertaken with the objective of establishing the normal reference values of certain haematological and biochemical parameters in Badri cattle maintained at Instructional Dairy Farm of Govind Ballabh Pant University of Agriculture and Technology (GBPUAT), Pantnagar, Uttarakhand, India. Healthy animals (40) aged between 4 to 8 years, having good body condition score (BCS) and 200-250 kg body weight were selected randomly and blood samples were collected. The haematological parameters such as haemoglobin concentration (Hb), packed cell volume (PCV), total erythrocyte count (TEC), total leucocyte count (TLC), erythrocyte sedimentation rate (ESR), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), differential leukocyte count (DLC), Neutrophil: lymphocyte (N: L), and absolute leucocyte count (ALC) were estimated. The biochemical parameters such as total protein, albumin, globulin, albumin: globulin (A:G), urea, creatinine, glucose, cholesterol, triglycerides, HDL (High density lipoprotein), LDL (Low density lipoprotein), total bilirubin, ALT (Alanine aminotransferase), AST (Aspartate transaminase), ALP (Alkaline phosphatase), GGT (Gamma-glutamyl transferase) and CK (Creatinine kinase) were also estimated. The normal haematological and biochemical values established in the present study could be helpful in the diagnosis of certain ailments in Badri cattle and the values would also be useful for academic purposes.