The abundances and distribution diamondoids were studied with a series of crude oil samples from Block 4 of the Mugald Basin in Sudan, to investigate the validity of various diamondoid parameters on the oil source, thermal maturity and biodegradation information. All crude oils of Block 4 were generated by lacustrine source in lower part of the Abu Gabra Formation as a whole, and can be further divided into two crude oil groups: oil group of the Neem-Azraq Uplift and oil group of K-3 well and B-1 well. They are all mature oils within oil window and four crude oils from the Neem-Azraq Uplift were slightly biodegraded. The concentrations of diamondoids in oils of K-3 well and B-1 well, especially the concentration of diamantanes, are higher than those of oils from the Neem-Azraq Uplift. The concentrations of diamondoids are mainly controlled by source of oil, while the influence of thermal maturity and biodegradation may be limited. The isomerization ratios as indicators of thermal maturity are inapplicable at the mature stage, but DMDI-2, 4,8/3,4-DMD and 1-EA/3-EA can clearly distinguish the two groups of crude oils. The concentration ratios of oils from K-3 and B-1 wells are significantly lower than these of oils from Neem-Azraq Uplift, and these concentration ratios including newly proposed parameters such as MAs/DMAs, DMAs/TMAs, D/MDs, MDs/DMDs and so on, are effective indicators on organofacies and oil group division within oil window.