Mytilus galloprovincialis is a marine mollusk belonging to the Bivalvia class. It has been distributed largely in Turkish shores and worldwide aquatic environments. Besides being known as an environmental pollution indicator, it is highly consumed as a food and has a high economic value. Due to their nutritional mechanisms by filtering water, they are affected by pollution in seawater and mussels can host-microbial diversity of environmental origin as well as pathogenic bacteria. Therefore, in this study, bacterial species found in Mediterranean mussels collected from the coastal stations of Istanbul [Rumeli Kavagi (RK), Kucukcekmece (KC)], and Izmir [(Foca (MF), Urla (MU)] were investigated and compared with microbiological and metagenomic analyses. According to microbiological analysis results, 34 mussel-associated Enterobacteriaceae and Vibrionaceae family members were identified. As a result of the culture-independent metagenomic analysis, taxonomic groups for each station were identified and compared based on Operational Taxonomic Unit data. For all stations, the most abundant bacterial genera were the unclassified bacterial genera. The total number of mussel-related total richness identified in all groups was 4889 (RK = 1605; KC = 1930; MF = 1508; and MU = 1125). According to the metagenomic data obtained in this study, different relative amounts of Lachnospiraceae and Bacteroidetes taxa groups were reported for all stations. The pathogenic bacterial genera identified by metagenomic analyses which may be significant for the public health are Arcobacter, Clostridium, Aeromonas, Vibrio, Escherichia_Shigella, Klebsiella, Campylobacter, Helicobacter, Pseudomonas, Morganella, Serratia, Corynebacterium, Enterococcus, Staphylococcus, Yersinia, Mycoplasma, Brucellaceae_unclassified, Pantoea, and Proteus.