Indica Leaf Extract Research Articles

Effect of Different Process Parameters on the formation of Silver Nanoparticles using Crude and Modified Neem (Azadirachta indica) Leaf Extracts

In recent times, the biosynthesis of nanoparticles, which has led to significant growth in the field of nanotechnology. The use of plant extracts has become an impetus in this field as it is a simple and eco-friendly method. This study was an attempt to study different parameters in biosynthesis of silver nanoparticles using Azadirachta indica (Neem) leaf extracts. Four different process parameters such as concentrations of neem leaf extract, types of neem leaf extract, mixing ratios and the reaction time period were investigated on the formation of silver nanoparticles. Initially, the formation of silver nanoparticles was detected by the visual observation. Then, the synthesized silver nanoparticles were characterized using UV-Visible spectroscopy and scanning electron microscopy (SEM). The change of color from yellow to reddish brown color confirmed the formation of silver nanoparticles. The silver surface plasmon resonance (SPR) band obtained in the expected visible range of UV-Visible spectroscopy confirmed the synthesis of the nanoparticles. SEM images showed that silver nanoparticles are roughly spherical and of uniform particle size, and the average particle size is 100 nm. Further, the maximum absorbance of SPR band was considerably varied with different process parameters used in the present study. The UV-Visible spectra of 2.5 g/100 mL of crude neem leaf extract without any dilution showed maximum absorbance in the expected range with the mixing ratio of (Neem and AgNO3) 1:8. However, the maximum absorbance of modified neem leaf extracts (pH 10) resulted lower in value than the crude extracts in the 20 times diluted sample with the mixing ratio of 1:9. Moreover, modified extract with UV radiation exposure increased the absorbance in the expected visible range. It concludes that fine tuning of the bioprocess parameters would enhance nanoparticle synthesis.

In vitro and in vivo Activities of Psidium guajava and Azadirachta indica Leaf Extracts and solvent Fractions against Salmonella Typhi

Typhoidal salmonella infections remain a challenge in the health care system in sub-Saharan Africa. Carrier status and advent of multi-drug resistant S. Typhi strains have necessitated the search for new drug leads. Hence, this study aims at investigating P. guajava and A. indica leaves for anti-salmonella activities. Guava and neem leaves were extracted by maceration in methanol and fractionated by solvent partitioning. In vitro activities were assessed by agar well diffusion and broth micro-dilution methods. Sixty male rats were randomized to 10 groups of 6 animals each for the in vivo experiments. Groups of rats except, normal control, were induced with 0.5McFarland of S. Typhi suspension orally. Treatment groups received 200 mg/kg body weight of extracts and fractions, and the control groups were treated with 14.29mg/kg body weight of ciprofloxacin and 1%v/v DMSO for 7 days post-infection. Biochemical parameters were determined spectrophotometrically. Hematological parameters were analyzed with automated hematology diagnostic machine. All fractions of P. guajava and three of A. indica inhibited S. Typhi growth with Zone of Inhibition (ZI) ranging from 11-15 mm. Active fractions inhibited 48.60-62.45% of S. Typhi biofilm formation at 25 mg/mL with Minimum Bactericidal Inhibitory Concentration (MBIC) of 0.39-12.5 mg/mL. All fractions improved body weight of treated rats and inhibited bacteremia at 44.75 and 95.94%. Hematological parameters improved in all fractions-treated rats. MDA was not significantly (p<0.05) altered in all groups. One fraction of P. guajava (ePg) lowered the elevated level in concentration of Nitric oxide (NO) while all fractions enhanced the lowered activity of SOD. Elevated (lactate dehydrogenase (LDH), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and bilirubin (BIL) were lowered by all fractions to various extents in treated rats. Fractions of P. guajava, and A. indica could be further considered for identification of active anti-salmonella principle(s).

Effect of Tamarindus indica L. Leaf Extract and Citrus sinensis L. Osbeck Peel Extract on Meloidogyne spp (Root Knot Nematodes) in Jos, Plateau State

Aim: The Aim of this study is to determine the effect of aqueous leaf extract of Tamarindus indica and peel extract Citrus sinensis on Root Knot Nematodes, in vitro.
 Study Design: The experiment was carried out using a completely randomized design.
 Place of Study: This study was carried out in the biology laboratory of the Department of Plant Science and Biotechnology, University of Jos, Nigeria.
 Methods: Extraction of phytochemicals from Tamarindus indica leaf and Citrus sinensis peels was carried out using an aqueous solvent. Tamarindus indica leaves and Citrus sinensis peels were collected within the province of Jos, and were dried at constant room temperature to a constant weight after which pulverized, sieved and soaked in water for 24 hours. The extraction was done using the cold maceration method. Eighty (80) second-stage juveniles of Meloidogyne spp. were exposed to the extracts at different concentrations (100, 80, 60, 40, and 20mg/ml) for 6, 12, 18, 24. 30, 32 and 48 hours and, each treatment was replicated 5 times. The average number of dead nematodes per concentration were determined and recorded as mean ± SEM with statistical value at P < 0.05 considered significant.
 Results: Results of phytochemical analysis showed that Alkaloids, Flavonoids, Tannins, Steroids, Phenols, and Cardioglycosides were present in T. indica leaf extracts while C. sinensis peels contains Flavonoids, Alkaloids, Saponins, Tannins, Phenols, and Resins. These phytochemicals present are suspected to be responsible for the nematicidal activities of the plants. Nematode mortality in the two treatment groups (T. indica and C. sinensis extracts) was highest at 100mg/ml concentration (48 hours of exposure); It was followed by 80mg/ml in decreasing order while the least mortality rate was recorded at 20mg/ml. Hence, as the concentration of the extracts were increased with increase in time, high nematode mortality was observed. Percentage mortality of treated nematodes was statistically significant (P < 0.05) for all concentrations of the extracts at the different time intervals when compared to the control. Statistical analysis showed that T. indica had significantly higher effect on Root-Knot nematodes mortality than C. sinensis at varying concentrations and time of exposure.
 Benefits of Results: Synthetic pesticides are the principal means used to control nematodes, but the natural products may provide a safe alternative. Hence, the active compounds (phytochemicals) in T. indica L. and C. sinensis L. can be harnessed to be used as biopesticides.
 Conclusion: The study suggests that extracts of T. indica and C. sinensis can be utilized in the control of root knot nematodes and may have the potential for the production of biopesticides that can be used for the control of nematodes as it displayed nematicidal activities.

Green synthesis of Ag–ZnO nanocomposite using Azadirachta indica leaf extract exhibiting excellent optical and electrical properties

In recent years, the green synthesis of nanoparticles has been the most explored area of research due to the need for eco-friendly, simple and non-toxic ways for synthesizing the nanoparticles with less waste production. In this paper, the synthesis of Ag–ZnO nanocomposite via hydrothermal method using neem leaf extracts in different solvents i.e., water and ethanol is studied. The characterization of thus prepared Ag–ZnO nanocomposites was done using X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM), Energy Dispersive X-Ray Spectroscopy (EDS), Fourier Transform Infrared spectroscopy (FTIR) and Photoluminescence (PL) to examine the structural, morphological and optical properties of the prepared nanocomposites. The I–V characteristics of the samples are also studied to determine their electrical properties. The decrease in bandgap and linear plot of I–V characterization of Ag–ZnO nanocomposite make it a suitable candidate for applications in various fields like solar cells, gas sensing, biosensors, photoanodes, photocatalysis and anti-bacterial applications etc.

Pharmacoinformatics and UPLC-QTOF/ESI-MS-Based Phytochemical Screening of Combretum indicum against Oxidative Stress and Alloxan-Induced Diabetes in Long-Evans Rats.

This research investigated a UPLC-QTOF/ESI-MS-based phytochemical profiling of Combretum indicum leaf extract (CILEx), and explored its in vitro antioxidant and in vivo antidiabetic effects in a Long–Evans rat model. After a one-week intervention, the animals’ blood glucose, lipid profile, and pancreatic architectures were evaluated. UPLC-QTOF/ESI-MS fragmentation of CILEx and its eight docking-guided compounds were further dissected to evaluate their roles using bioinformatics-based network pharmacological tools. Results showed a very promising antioxidative effect of CILEx. Both doses of CILEx were found to significantly (p < 0.05) reduce blood glucose, low-density lipoprotein (LDL), and total cholesterol (TC), and increase high-density lipoprotein (HDL). Pancreatic tissue architectures were much improved compared to the diabetic control group. A computational approach revealed that schizonepetoside E, melianol, leucodelphinidin, and arbutin were highly suitable for further therapeutic assessment. Arbutin, in a Gene Ontology and PPI network study, evolved as the most prospective constituent for 203 target proteins of 48 KEGG pathways regulating immune modulation and insulin secretion to control diabetes. The fragmentation mechanisms of the compounds are consistent with the obtained effects for CILEx. Results show that the natural compounds from CILEx could exert potential antidiabetic effects through in vivo and computational study.

Ethnobotanical Survey and Evaluation of Anti-Salmonella Potentials of Commonly Used Plants for Typhoid Treatment in Ogbomoso, Oyo State, Nigeria

This study assessed the sensitivity of S. typhi to extracts of commonly used plants in treatment of typhoid in Ogbomoso, Oyo State, Nigeria. Ethnobotanical survey was conducted using a structured questionnaire. Powdered leaves of plants were extracted by maceration in distilled water. In vitro sensitivity of S. typhi to the extracts were assessed using agar well diffusion method. Groups of rats were infected orally with 0.5 McFarland suspension of S. typhi. Bacteremia in the animals was monitored by plating on Salmonella-Shigellar agar. Biochemical parameters were determined spectrophotometrically. Histological analysis was performed using H&amp;E staining method. S. typhi was sensitive only to P. guajava and A. indica leaf extracts. V. amygladina (90.31%) and C. papaya (92.26%) showed highest percentage inhibition of S. typhi comparable with ciprofloxacin (99.86%). Haematological parameters varied significantly (p&lt;0.05) among the groups. Antioxidant status of rats, total protein, albumin and the corresponding globulin level increased significantly (p&lt;0.05) in the groups treated with P. guajava and A. indica. C. papaya and C. citratus ameliorated the histopathological damages observed in the liver and intestine of S. typhi infected rats. The studied plants have direct activities against S. typhi, thus reflecting the reason for their combination in traditional system of medicine.

Antimicrobial activity of Azadirachta indica (Neem) leaf extract on gram positive and gram-negative bacteria

Infectious diseases produced by bacteria are an urgent healthcare concern worldwide. Several infections in humans are caused by bacterial agents such as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Staphylococcus aureus. The current development of resistance to antibiotics and accompanying toxicity issues is causing a rise in studies into the antibacterial role of plants against resistant strains due to relative effectiveness and safety. The current work was done to check the antibacterial activity of Azadirachta indica leaves (Neem) along with the green synthesis of silver nanoparticles. Methanol and Acetone extracts of Neem leaves were tested against Klebsiella oxytoca and Staphylococcus aureus, both of which are resistant to antibiotics. Silver nanoparticle synthesized in methanol was very effective against Klebsiella oxytoca (13.5mm).

Antifungal Activity of Gueira senegalensis L. and Mangifera indica L. on Sorghumloose smut pathogen (Sporisorium cruentum (KUHN) POTTER)

The objective of the research was to determine the antifungal activities of Guiera senegalensis L. and Mangifera indica L. leave extracts against most frequently occurring sorghum pathogen of the loose smut (Sporisorium cruentum) in Nigeria. Methanolic extracts from Guiera senegalensis and Mangifera indica L. were tested at concentrations of 250, 125, 62.5, and 31.2mg/ml as potential sources of antifungal agents for Sporisorium cruentum. All extracts exhibited moderate to good activities on the tested fungi with minimum inhibitory concentrations (MICs). The test organism was sensitive to the G. senegalensis extracts, with 14mm and 10mm in diameter at 250mg/ml and 125mg/ml, respectively. The test organism was sensitive to M. indica leaf extract with 12mm in diameter at 250mg/ml. The results showed that both extracts exhibited inhibitory effects at different concentrations against the test organism. Therefore, it is concluded that extracts of G. senegalensis and M. indica could be used in controlling the fungal pathogen of sorghum loose smut (Sporisorium cruentum).

Techno-Economic Analysis on the Production of Copper Oxide Nanoparticles by Green Synthesis Method using Abultion indicum Leaf Extract on an Industrial Scale

The purpose of this study was to evaluate the economic feasibility of producing copper oxide (CuO) nanoparticles using the green synthesis method on an industrial scale for 10 years by evaluating from an engineering and economic perspective. Various economic parameters are used to analyze economic viability, including Gross Profit Margin (GPM), Cumulative Net Present Value (CNPV), Payback Period (PBP), as well as economic variations in sales, taxes, raw materials, labor wages, and utilities to ensure project feasibility. Technical analysis to produce 100 kg of CuO nanoparticles per day requires a total production cost of 220,322.850.00 IDR and the gross profit margin is 16,721,250.000,00 IDR per year. PBP analysis shows that the investment will be profitable after more than three years. This project can compete with PBP capital market standards because of the short investment return. The profit is relatively economical, so this project can be run for 10 years under ideal conditions. This research is expected to be a reference for technical and economic analysis of industrial-scale production of CuO nanoparticles

Comparative analysis of Qualitative and Quantitative Phytochemical screening, in vitro antioxidant and antibacterial activity of Mangifera indica and Artocarpus heterophyllus

Medicinal plants have known to play a wide role in the development of human culture. Artocarpus heterophyllus and Mangifera indica are the well-known evergreen tropical fruiting tree families abundantly grown in Asia. Different parts of both the trees such as bark, leaves, fruits, and flowers are used in traditional medicine. The present study aims to screen the qualitative and quantitative phytochemical present, in-vitro antioxidant, and anti-microbial activity of Artocarpus heterophyllus and Mangifera indica leaf extracts and combined extract of both the leaves. The qualitative phytochemical results indicated the presence of phytochemicals such as alkaloids, flavonoids, phytosterols, tannins, and saponins. The quantitative phytochemical results revealed that M.indica has high phenolic and flavonoid content. The Combined extract showed significant antioxidant activity when compared to both the extracts. All the extracts showed significant antimicrobial activity.

Azadirachta indica Leaf Extract as Green Corrosion Inhibitor for Reinforced Concrete Structures: Corrosion Effectiveness against Commercial Corrosion Inhibitors and Concrete Integrity.

The construction industry has extensively demanded novel green inhibition strategies for the conservation and protection of carbon steel-reinforced concrete structures. For the first time, the effect of Azadirachta indica leaf extract (Neem) as a potential corrosion inhibitor of carbon steel in reinforced concrete under corrosion in saline simulated media was evaluated. To assess the corrosion inhibition behavior of the Neem natural organic extract, three inorganic commercial inhibitors were tested to compare following the criteria established by Stratful for half-cell potential under a simulated chloride environment. Moreover, the effect of concrete integrity by the Neem treatment was recorded after different temperature conditions, slump, weight alteration, air content, compressive strength, and chloride-ions penetration. The results suggested that the Neem treatments did not alter the concrete integrity and the physicochemical parameters. We reached a promoted long-term corrosion protection of 95% after 182 days of evaluation. Thus far, our current results open up a new promising “green” road to the conservation of carbon steel in reinforced concrete for the construction industry.

Selective N-Alkylation of Amines with DMC over Biogenic Cu-Zr Bimetallic Nanoparticles.

Herein, we report the green synthesis of copper–zirconium bimetallic nanoparticles (Cu–Zr BNPs) from aqueous solutions using Azadirachta indica leaf extract as a reducing and stabilizing agent. The CuO, ZrO2 NP, and Cu–Zr BNP samples were characterized by X-ray diffraction and Fourier transform infrared (FTIR) spectroscopy, and the morphologies of the samples were analyzed by high-resolution transmission electron microscopy (HR-TEM) with selected area electron diffraction analysis (SAED). The synthesized Cu–Zr BNPs have been employed as efficient catalysts for the selective N-methylation of aromatic and aliphatic amines with dimethyl carbonate. The effect of process conditions on the percentage conversion of benzylamine with dimethyl carbonate as a model reaction has been investigated. The Cu–Zr bimetallic nanoparticle catalytic system in a 1:2 molar ratio was able to convert amines into the corresponding N-methylated amines with a selectivity up to 91% at 180 °C in 4 h. The analysis of catalytic reusability confirmed that the reported heterogeneous catalyst can be used for five consecutive cycles without much loss in activity. Thus, the current protocol can be considered as a simpler, reproducible, and environmentally benign approach for N-methylation of amines.

Effect of cashew (Anacardium occidentale) nut shell stored and fresh extracts on cowpea bruchid, Callosobruchus maculatus (Fabricius.) (Coleoptera: Chrysomelidae)

Effect of cashew (Anacardium occidentale) nut shell stored and fresh extracts on cowpea bruchid, Callosobruchus maculatus (Fabricius.) (Coleoptera: Chrysomelidae)

Tamarindus indica Leaf Extract Mediated Au NPs for Antimicrobial Treatment of Infectious Skin Wounds in Postoperative Wound Care Applications

The main cause of delay in the wound healing process is usually the infection due to pathogens such as Pseudomonas aeruginosais. The current study reports the green fabrication of gold nanoparticles (Au NPs) using Tamarindus indica leaf extract. The prepared Au NPs have displayed significant antimicrobial activities. Also, Au NPs activity alone and when combined with tetracycline was studied on the inoculated wounds with P. aeruginosain in mice. A total of 20 mice were anesthetized and deep skin wounds were made on the back of all the mice. Then, a suspension containing bacteria was applied to wounds. The wound infection was studied by measuring the total microbial count as well as monitoring the wound healing macroscopically. Treatments were performed to all the groups on the wound bed topically using Au NPs alone, Au NPs with tetracycline (Au NPs + tetracycline), tetracycline alone and normal saline in the control group. The Au NPs + Tetracycline group has attained 100% closure of the wound on the 9th day. The wound contraction percentage of animals treated with Au NPs (98%) was noticed to be almost similar with the percentages of normal saline (79%) and tetracycline (99%) animal groups. In addition, all the groups treated with Au NPs + Tetracycline, tetracycline alone and Au NPs alone showed decreased bacterial concentration on the wound surface by the 9th day in comparison to the control group. Similarly, a significant decrease (P &lt; 0.001) was observed in the bacterial count of the groups treated with Au NPs+Tetracycline, tetracycline and Au NPs on comparison with the control group at all the time periods. Treatment with Au NPs + Tetracycline is highly effective than tetracycline and Au NPs alone for the reduction of bacterial load with an increase in the wound macroscopic closure. Therefore, the above results support the application of Au NPs combined with an antimicrobial medicine for treating the skin wound infections.

Efficacy of a simply resting box baited with crude fruit and leaf ethanol extracts of Phytolaccadodecandra (L' Herit) in capturing and killing of indoor mosquitoes (Diptera: Culicidae) at Korando, Western Kenya.

Effective capture and elimination of indoor resting mosquito population is important in the fight against mosquito borne diseases. This study aimed at evaluating the efficacy of a simply resting box baited with crude fruit and leaf ethanol extracts of Phytolacca dodecandra in attracting and killing indoor mosquitoes at Korando, Western Kenya. The study was conducted in three phases: pre-intervention, intervention and post intervention. Simple resting boxes made from galvanized wire frame measuring 30 cm × 30 cm × 30 cm, covered in blue and black tunic in and out and lined with carton boards were used. The boxes were baited with socks with strong human odour and 80 ml/100mls (e/w) solution of either crude ethanol fruit or leaf extracts of P. dodecandra, ethanol leaf extracts of Azadiracta indica or Deltamethrin. Deltamethrin and Azadiracta indica were used as positive and water as negative control. The treatments were applied at the intervention phase only. The boxes were left overnight in the houses and mosquitoes collected by 6.30 h. It was observed that more Culicines than Anopheline were captured irrespective of phase or treatment used. Mosquito densities reduced with phase of activity. P. dodecandra leaf extracts killed more mosquitoes than fruit or A. indica leaf extracts though the number were less than that of Deltamethrin or WHO threshold of >80% mortality. In conclusion, the simple resting boxes were effective in collecting and killing indoor mosquitoes though lethality did not matched the WHO threshold. With improved structural set up and use of pure extracts of P. dodecandra, the resting boxes can serve as effective tools for capture, elimination and management of mosquito borne diseases.

Anti-inflammatory and Antioxidant Effects of Pluchea indica Leaf Extract in TNF-α-Induced Human Endothelial Cells

Pluchea indica is a shrub plant found in mangrove forests. The leaves are consumed as food and herbal tea and exhibit various biological effects, such as antioxidant and anti-inflammatory activities, in macrophages and animal models. However, the inhibitory activity of P. indica leaf extract on vascular inflammation remains unknown. Therefore, this study investigated the effect of an ethanol extract from P. indica herbal tea leaves (PIE) on tumor necrosis factor-α (TNF-α)-induced human vascular endothelial EA.hy926 cells. The cytotoxic effect of PIE was determined by thiazolyl blue tetrazolium bromide assays. PIE at concentrations of 12.5 - 50 µg/mL did not show significant cytotoxicity, whereas PIE at concentrations ≥ 100 µg/mL decreased cell viability. PIE inhibited the production of reactive oxygen species (ROS) in TNF-α-stimulated endothelial cells. To evaluate the PIE’s anti-vascular inflammatory activity, the protein expression of cell adhesion molecules, including intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), was determined by western blot. PIE significantly decreased TNF-α-induced ICAM-1 and VCAM-1 expression in a concentration-dependent manner. Furthermore, PIE upregulated heme oxygenase-1 (HO-1) in a concentration- and time-dependent manner. Inhibiting the activity of HO-1 by tin protoporphyrin IX significantly blocked the suppressive effect of PIE on ICAM-1 but not VCAM-1 expression. Therefore, PIE exerts anti-inflammatory activity on vascular endothelial cells, at least in part, by suppressing ROS production and the induction of HO-1. The obtained data suggest that PIE is a promising substance for developing therapeutic agents or as an ingredient of functional food. HIGHLIGHTS Pluchea indica leaf extract (PIE) at non-toxic doses inhibited ICAM-1 and VCAM-1 in TNF-α-induced human vascular endothelial cells PIE suppressed the production of ROS in TNF-α-stimulated endothelial cells PIE exerts anti-inflammatory activity on vascular endothelial cells mediated partly through the upregulation of HO-1

Investigations on Antibacterial Potency of Azadirachta Indica Leaf Extracts against Some Resistant Bacteria

Resistance to antibiotics in pathogens responsible for causing infectious diseases has become a matter of great public health concern internationally and India is no exception to it. Widespread use and availability of practically all the antimicrobials across the counter and incompletion of the dose is supposed to be one of the major reasons for this. It is observed that some plant extracts seem to potentiate the activity of antibiotics against resistant bacterial strains. So the objective of the study was to use some natural products as an alternate to the conventional treatment against antibiotic resistance. The effects of crude extracts of leaves of Azadirachta indica against resistant strains of pathogenic S. aureus, E. coli and K. Pneumoniae were investigated using the agar well diffusion method. It was observed that the growth of all the isolates were inhibited to varying degrees with gram-positive more susceptible than gram-negative bacteria. Hexane extract was more effective, producing larger zones of growth inhibition sizes and smaller MIC, than the aqueous and ethyl acetate extracts. The MIC values ranged from 0.5 - 4 mg/ml. The ability of the crude extracts to inhibit the growth of such resistant pathogenic bacterial strains that were used in this study is an indication that the neem leaves extract has the potential and can be used as a source for new broad spectrum oral antibiotics. The result obtained in this study validates the use of the neem leaves extract in traditional medicine to treat infectious conditions.

Formulation and Evaluation of niosome loaded topical gel using Acalypha indica leaf extract

Formulation and Evaluation of niosome loaded topical gel using Acalypha indica leaf extract

Dietary supplementation effects of the levels of Neem (Azadirachta indica) leaf extracts as phytogenic feed additive on the growth performance in broiler chicks

The effects that Neem leaf extract on overall growth, feed conversion ratio (FCR), dressed weight and organs weight of broiler chickens to justify its inclusion in the diet of growing broiler chickens. 120 day old chicks divided into four treatment groups T1, T2, T3 and T4 of thirty (30) birds each were administered clean drinking water with 3gm, 4gm, 5gm and 0g neem extract respectively. Impacts of neem extract treatments on growth indices suggested that Neem extract favored growth with final body weight range of 1709gm and 1763gm. Organ weights showed no deviation from standard values for healthy broiler birds. Highest growth was observed in group T3 (1763.6±141.37gm) followed by T1, T2 and lowest was in T4 group (1565.4±161.25gm). Birds on 5gm of Neem extract in their drinking water out performed birds in other treatments in assessed growth rate indices. We therefore conclude that 5gm of Neem extract in their drinking water growing broiler birds encourages healthy growth and may serve as supplements for antibiotics, especially when birds are raised in areas with minimal access to veterinary service.&#x0D; Asian J. Med. Biol. Res. March 2021, 7(1): 64-68

Effects of Supplementation with Dried Neem Leaf Extract on Lipid Peroxidation and Antioxidant Enzyme mRNA Expression in the Pectoralis Major Muscle of Broiler Chickens.

This study was conducted to evaluate the effects of dietary supplementation of dried neem (Azadirachta indica) leaf extract (DNE) on lipid peroxidation and the expression of genes encoding mRNAs in antioxidant enzymes in the pectoralis major muscle of chickens. A total of 24 male broiler chickens (ROSS308) were divided into three groups (n=8) at 21 days of age. The control group of chickens was fed a basal diet, and the remaining two groups of chickens were fed a basal diet supplemented with DNE at a concentration of 0.5% or 2.0% until 35 days of age. Growth performance (body weight, weight gain, feed intake, and feed conversion ratio) and tissue weights did not differ among the three groups. The 2.0% DNE-supplemented diet decreased the muscle malondialdehyde content, a marker of lipid peroxidation, and drip loss compared to the control chickens. In addition, the expression of genes encoding mRNAs of antioxidant enzymes (i.e., Cu/Zn-superoxide dismutase, Mn-superoxide dismutase, glutathione peroxidase 7, and catalase) were higher in the pectoralis major muscle of chickens fed the 2.0% DNE-supplemented diet than in the control chickens. Therefore, DNE supplementation increased the expression of genes encoding mRNAs in antioxidant enzymes and reduced lipid peroxidation and drip loss in the pectoralis major muscle of broiler chickens.